| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-POGZ-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001438 |
| 产品用途: | 3' AAVLINK plasmid for POGZ expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | POGZ |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-POGZ-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-POGZ-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-POGZ-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-POGZ-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-POGZ-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-POGZ-FLAG 6168 bp DNA circular SYN 13-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6168)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6168
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..2869
/codon_start=1
/label=POGZ
/translation="GAQSPKNLS*HQVVVVVVELAKRSSCL*RSFE*YCLLYAAIQNRQ
LNTSEIPSDVFAVGFDVSRPPRGRI*RANI*ALRQKRNWLSGC*PSANNSYL*MRRPCS
RRPPK*DVLWKGGLRSPMSGLCVSCCGTT*LPMPGELWPTPYLRM*QRMQDSSLILYNG
RFTTRTYPCL*LWLLMRSLCSWIQRC*AVMIERRMPCRQWAQGNLGVM*S*PFWQMALS
FPPWFSTEGRWISLLTCQTPYC*RQRRVATVMTRSWSCGQLECGRSTQLASAAKACL*W
TVIALTCQKRYWLCLVPLALCLQWSQQAVAPKFSH*MYASKELSRTSCIKNGRNRLGKW
QILHVILMSCFSWCLSGWVKC*VSLGTVQS*FSAPSWWLVFCLAPMATLTHLQEMLTCR
RS*LPP*RSN*S*VGNILSLPLHDPDHLLKRQLSLKVFTSSLRVKVRPSLSMALKKLT*
I*WR"
misc_feature 2676..2681
/label=SDS
CDS 2876..2941
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 2957..3388
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3101..3106
/label=SDS
repeat_region 3431..3571
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3431..3560
/label=AAV2 ITR
rep_origin 3646..4101
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4383..4487
/gene="bla"
/label=AmpR promoter
CDS 4488..5348
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5519..6107
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggagcccag tcacccaaga
1501 acctgagcta gcatcaggtg gtggtggtag tggtggagtt ggcaaaaagg agcagctgtc
1561 tgtgaagaag cttcgagtag tactgtttgc tctatgctgc aatacagaac aggcagctga
1621 acacttccga aatccccagc gacgtattcg ccgttggctt cgacgtttcc aggcctccca
1681 gggggagaat ctagagggca aatatctgag ctttgaggca gaagagaaac tggctgagtg
1741 ggtgctaacc cagcgcgaac aacagctacc tgtaaatgag gagaccttgt tccagaaggc
1801 caccaaaata ggacgttctt tggaaggggg gtttaagatc tcctatgagt gggctgtgcg
1861 tttcatgctg cggcaccacc tgactcccca tgcccggcga gctgtggccc acaccctacc
1921 taaggatgta gcagagaatg caggactctt cattgatttt gtacaacggc agattcacaa
1981 ccaggactta cccttgtcta tgattgtggc tattgatgag atctctttgt tcctggatac
2041 agaggtgctg agcagtgatg atcgaaagga gaatgccctg cagacagtgg gcacagggga
2101 accttggtgt gatgtagtcc tagccattct ggcagatggc actgtccttc ccaccctggt
2161 tttctacaga gggcagatgg atcagcctgc taacatgcca gactccatat tgctagaggc
2221 aaaggagagt ggctacagtg atgacgagat catggagctg tggtcaactc gagtgtggca
2281 gaagcacaca gcttgccagc gcagcaaagg catgcttgtg atggactgtc atcgcactca
2341 cttgtcagaa gaggtactgg ctatgcttag tgcctctagc actttgcctg cagtggtccc
2401 agcaggctgt agctccaaaa ttcagccatt agatgtatgc atcaaaagaa ctgtcaagaa
2461 cttcctgcat aaaaaatgga aggaacaggc tcgggaaatg gcagatactg catgtgattc
2521 tgatgtcctg cttcagctgg tgcttgtctg gctgggtgaa gtgctaggtg tcattgggga
2581 ctgtccagag ctagttcagc gctccttcct ggtggctagt gttctgcctg gccccgatgg
2641 caacattaac tcacctacaa gaaatgctga catgcaggag gagctaattg cctccctaga
2701 ggagcaactg aagctgagtg gggaacattc tgagtcttcc actccacgac ccagatcatc
2761 tcctgaagag acaattgagc ctgaaagtct tcaccagctc tttgagggtg aaagtgagac
2821 cgagtctttc tatggctttg aagaagctga cctagatctg atggagattt gtacagacta
2881 caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa
2941 gtaagaattc tgatgactcg agataatcaa cctctggatt acaaaatttg tgaaagattg
3001 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
3061 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
3121 ttagttcttg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
3181 cggctgttgg gcactgacaa ttccgtggtg tttatttgtg aaatttgtga tgctattgct
3241 ttatttgtaa ccatctagct ttatttgtga aatttgtgat gctattgctt tatttgtaac
3301 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
3361 tcagggggag atgtgggagg ttttttaaag atctggagac cggtgtggca cgtgcggacc
3421 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
3481 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
3541 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
3601 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
3661 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
3721 ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
3781 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
3841 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
3901 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
3961 ctggaacaac actcaactct atctcgggct attcttttga tttataaggg attttgccga
4021 tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
4081 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
4141 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
4201 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
4261 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
4321 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
4381 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
4441 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
4501 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
4561 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
4621 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
4681 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
4741 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
4801 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
4861 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
4921 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
4981 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
5041 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
5101 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
5161 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
5221 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
5281 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
5341 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
5401 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
5461 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
5521 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
5581 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
5641 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
5701 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
5761 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
5821 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
5881 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
5941 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
6001 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
6061 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
6121 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
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