| 质粒名称: | pAAVLINK-EF1a-HA-5'-ARHGEF10 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001590 |
| 产品用途: | 5' AAVLINK plasmid for ARHGEF10 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ARHGEF10 |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-ARHGEF10
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-ARHGEF10载体质粒基本信息
pAAVLINK-EF1a-HA-5'-ARHGEF10质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-ARHGEF10载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-ARHGEF10质粒载体简介
pAAVLINK-EF1a-HA-5'-ARHGEF10质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-ARHGEF10 5867 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5867)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5867
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..3029
/codon_start=1
/label=ARHGEF10
/translation="DQREPLPPAPAENEMKYDTNNNEEEEGEQFDFDSGDEIPEADRQA
PSAPETGGAGASEAPAPTGGEDGAGAETTPVAEPTKLVLPMKVNPYSVIDITPFQEDQP
PTPVPSAEEENVGLHVPCGYLVPVPCGYAVPSNLPLLLPAYSSPVIICATSLDEEETPE
VTEDRQPNSLSSEEPPTSEDQVGREDSALARWAADPANTAWMENPEEAIYDDVPRENSD
SEPDEMIYDDVENGDEGGNSSLEYGWSSSEFESYEEQSDSECKNGIPRSFLRSNHKKQL
SHDLTRLKEHYEKKMRDLMASTVGVVEIQQLRQKHELKMQKLVKAAKDGTKDGLERTRA
AVKRGRSFIRTKSLIAQDHRSSLEEEQNLFIDVDCKHPEAILTPMPEGLSQQQVVRRYI
LGSVVDSEKNYVDALKRILEQYEKPLSEMEPKVLSERKLKTVFYRVKEILQCHSLFQIA
LASRVSEWDSVEMIGDVFVASFSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFL
KQEQEASPDRTTLYSLMMKPIQRFPQFILLLQDMLKNTSKGHPDRLPLQMALTELETLA
EKLNERKRDADQRCEVKQIAKAINERYLNKLLSSGSRYLIRSDDMIETVYNDRGEIVKT
KERRVFMLNDVLMCATVSSRPSHDSRVMSSQRYLLKWSVPLGHVDAIEYGSSAGTGEHS
RHLAVHPPESLAVVANAKPNKVYMGPGQLYQDLQNLLHDLNVIGQITQLIGNLKGNYQN
LNQSVAHDWTS"
misc_feature 889..894
/label=SDS
misc_feature 925..930
/label=SDS
misc_feature 1035..1040
/label=SDS
misc_feature 1071..1076
/label=SDS
misc_feature 1153..1158
/label=SDS
misc_feature 2298..2303
/label=SDS
misc_feature 2371..2376
/label=SDS
intron 3030..3070
/label=Splicing Donor
misc_feature 3074..3107
/label=lox2272-JT1571
repeat_region 3152..3270
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3152..3259
/label=AAV2 ITR
rep_origin 3345..3800
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4082..4186
/gene="bla"
/label=AmpR promoter
CDS 4187..5047
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5218..5806
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctgacc agcgagagcc cctgcctccc gctcctgcag aaaatgaaat
781 gaaatatgat accaataata atgaagagga agagggagaa cagttcgatt ttgacagtgg
841 agatgaaatc ccagaagcgg acagacaggc cccctccgcc cctgagacag gaggtgctgg
901 agccagtgaa gcccctgcac ccacaggagg tgaggatgga gctggagcag aaaccacccc
961 agtggcagag cctactaagc tggtgctccc gatgaaagtc aacccatatt ctgtcatcga
1021 catcacgcca ttccaggagg accagccgcc cacccccgtg cccagcgctg aggaggagaa
1081 tgtgggtctc catgtgccct gcgggtactt ggtgcctgta ccctgcggct atgcggtgcc
1141 ctccaacctg cccctcctgc tgcccgccta ctccagcccg gtcatcatct gcgccacgtc
1201 cctggacgaa gaagaaacac cagaagtcac agaagatcgc cagcccaatt ctctgagttc
1261 cgaggagcct ccaaccagtg aagatcaagt cggtcgagag gacagcgcac ttgcccgctg
1321 ggccgcagac ccggccaaca cagcctggat ggagaatcca gaggaagcaa tttacgatga
1381 cgttccaagg gaaaactcag actctgaacc agatgaaatg atttatgatg atgttgagaa
1441 tggggatgaa ggtggaaaca gctccttgga atacggatgg agttcgagtg aatttgaaag
1501 ttacgaagag cagagtgact cggagtgcaa gaatgggatt cccaggtcct tcctgcgcag
1561 caaccacaaa aagcaacttt ctcatgacct aacccgttta aaggagcact atgagaaaaa
1621 gatgagagat ttgatggcaa gcacggtggg cgtggtggag attcagcagc tcaggcagaa
1681 gcatgaactg aagatgcaga agctcgtgaa ggccgcgaag gacggcacca aggacgggct
1741 ggagaggacc agggcagccg tgaagagggg ccgctccttc atcaggacca agtctctcat
1801 cgcacaggat cacagatctt ctcttgagga agaacagaat ttgttcattg atgttgactg
1861 caagcacccg gaagccatct tgaccccgat gcccgagggt ttatctcagc agcaggttgt
1921 aagaagatat atactgggtt cagttgtcga cagtgaaaag aactacgtag atgctcttaa
1981 gaggattttg gagcaatatg agaagccgct gtctgagatg gagccaaagg ttctgagtga
2041 gaggaagctg aagacggtgt tctaccgagt caaagagatc ctgcagtgcc actcgctatt
2101 tcagatcgcg ctggccagcc gcgtttccga gtgggactcc gtggaaatga taggcgatgt
2161 cttcgtggct tcgttttcta agtccatggt gctggatgca tacagtgaat atgtgaacaa
2221 tttcagcaca gccgtggcag tcctcaagaa aacatgtgcc acaaagcccg cttttcttga
2281 atttttaaag caggaacagg aggccagccc cgatcgaacc acgctctaca gcctgatgat
2341 gaagcccatc cagaggttcc cacagttcat cctcctgctc caggacatgc tgaagaacac
2401 ctccaaaggc caccccgaca ggctgcctct tcagatggcc ctgacagagc tcgaaacact
2461 agcagagaag ttaaatgaaa gaaagagaga tgctgatcaa cgctgtgaag tgaagcaaat
2521 agccaaagcc ataaacgaaa gatacctgaa caagcttctc agcagtggaa gccgatacct
2581 cattcgatca gatgatatga tagaaacagt ttacaacgac agaggagaga ttgttaaaac
2641 caaagaacgc cgagtcttca tgttaaatga tgtgttaatg tgtgccaccg tcagctcacg
2701 cccctctcat gacagccgtg tgatgagcag ccagaggtac ttgctgaagt ggagcgttcc
2761 actgggacat gtggacgcca tcgagtatgg cagcagcgca ggcacgggcg agcacagcag
2821 gcaccttgcc gttcacccgc cggagagcct ggccgtggtt gctaacgcga aaccaaacaa
2881 agtttacatg gggccaggac aactgtatca agatttacaa aacttgttgc atgacttaaa
2941 tgtaattggc caaatcactc agctgatagg aaaccttaaa ggaaactatc agaacttaaa
3001 ccagtcagta gcccatgact ggacatcagg taagtatcaa ggttacaaga caggtttaag
3061 gagaccaata agctattatt cgtataggat actttatacg aagttattaa gttatggaga
3121 ccggtgtggc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact
3181 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccggcctcag
3241 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
3301 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
3361 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
3421 cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
3481 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
3541 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
3601 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
3661 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
3721 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
3781 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
3841 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
3901 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
3961 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
4021 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
4081 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
4141 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
4201 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
4261 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
4321 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
4381 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
4441 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
4501 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
4561 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
4621 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
4681 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
4741 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
4801 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
4861 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
4921 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
4981 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
5041 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
5101 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
5161 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
5221 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
5281 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
5341 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
5401 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
5461 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
5521 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
5581 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
5641 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
5701 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
5761 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
5821 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
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