| 质粒名称: | pAAVLINK-EF1a-5'-NRXN2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001398 |
| 产品用途: | 5' AAVLINK plasmid for NRXN2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | NRXN2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-5'-NRXN2载体质粒基本信息
pAAVLINK-EF1a-5'-NRXN2质粒图谱载体图谱和pAAVLINK-EF1a-5'-NRXN2载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-5'-NRXN2质粒载体简介
pAAVLINK-EF1a-5'-NRXN2质粒序列载体序列
LOCUS pAAVLINK-EF1a-5'-NRXN2 6488 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6488)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6488
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
regulatory 707..716
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 713..3628
/codon_start=1
/label=NRXN2
/translation="MASGSRWRPTPPPLLLLLLLALAARADGLEFGGGPGQWARYARWA
GAASSGELSFSLRTNATRALLLYLDDGGDCDFLELLLVDGRLRLRFTLSCAEPATLQLD
TPVADDRWHMVLLTRDARRTALAVDGEARAAEVRSKRREMQVASDLFVGGIPPDVRLSA
LTLSTVKYEPPFRGLLANLKLGERPPALLGSQGLRGATADPLCAPARNPCANGGLCTVL
APGEVGCDCSHTGFGGKFCSEEEHPMEGPAHLTLNSEVGSLLFSEGGAGRGGAGDVHQP
TKGKEEFVATFKGNEFFCYDLSHNPIQSSTDEITLAFRTLQRNGLMLHTGKSADYVNLS
LKSGAVWLVINLGSGAFEALVEPVNGKFNDNAWHDVRVTRNLRQHAGIGHAMVNKLHYL
VTISVDGILTTTGYTQEDYTMLGSDDFFYIGGSPNTADLPGSPVSNNFMGCLKDVVYKN
NDFKLELSRLAKEGDPKMKLQGDLSFRCEDVAALDPVTFESPEAFVALPRWSAKRTGSI
SLDFRTTEPNGLLLFSQGRRAGGGAGSHSSAQRADYFAMELLDGHLYLLLDMGSGGIKL
RASSRKVNDGEWCHVDFQRDGRKGSISVNSRSTPFLATGDSEILDLESELYLGGLPEGG
RVDLPLPPEVWTAALRAGYVGCVRDLFIDGRSRDLRGLAEAQGAVGVAPFCSRETLKQC
ASAPCRNGGVCREGWNRFICDCIGTGFLGRVCEREATVLSYDGSMYMKIMLPNAMHTEA
EDVSLRFMSQRAYGLMMATTSRESADTLRLELDGGQMKLTVNLDCLRVGCAPSKGPETL
FAGHKLNDNEWHTVRVVRRGKSLQLSVDNVTVEGQMAGAHMRLEFHNIETGIMTERRFI
SVVPSNFIGHLSGLVFNGQPYMDQCKDGDITYCELNARFGLRAIVADPVTFKSRSSYLA
LATLQAYASMHLFFQFKTTAPDGLLLFNSGNGNDFIVIELVK"
misc_feature 1528..1533
/label=SDS
misc_feature 1566..1571
/label=SDS
misc_feature 1956..1961
/label=SDS
intron 3629..3669
/label=Splicing Donor
misc_feature 3673..3706
/label=lox2272-JT1571
repeat_region 3751..3891
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3751..3880
/label=AAV2 ITR
rep_origin 3966..4421
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4703..4807
/gene="bla"
/label=AmpR promoter
CDS 4808..5668
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5839..6427
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatggcgtc
721 cgggagccgg tggcggccga caccgccgcc gctgctgttg ctgctgctgc tggcgctggc
781 ggcgcgcgcg gacggcctgg agttcggcgg cggccccggg cagtgggctc gctacgcgcg
841 ctgggcgggc gcggcgagca gcggcgagct cagcttcagc ctgcgcacca acgccacgcg
901 cgcgctgctg ctctacctgg acgacggcgg cgactgcgac ttcctggagc tgctgctggt
961 ggacggccgc ctgcggctgc gcttcacgct ttcgtgcgcc gagccggcca cgctgcagct
1021 ggacacgccg gtggccgacg accgctggca catggtgctg ctgacccgcg acgcgcgccg
1081 cacggcgctg gcggtggacg gcgaggcccg cgccgccgag gtgcgctcca agcggcgcga
1141 gatgcaggtg gccagcgacc tgttcgtggg cggcatcccg cccgacgtgc gcctctcggc
1201 gcttacgctg agcaccgtca agtacgagcc gcccttccgc ggcctcttgg ccaacctgaa
1261 gctgggcgag cggccccccg cgctgctggg cagccagggc ctgcgcggcg ccaccgccga
1321 cccgctgtgc gcgcccgcgc gcaacccctg cgccaacggc ggcctctgca ccgtgctggc
1381 ccccggcgag gtgggctgcg actgcagcca cacgggcttc ggcggcaagt tctgcagcga
1441 agaggagcac cccatggaag gtccggctca cctgacgtta aacagcgaag tagggtcctt
1501 actgttctcc gagggggggg ccgggagagg aggagccggc gatgtgcacc agccaacaaa
1561 aggcaaggag gagtttgtgg cgaccttcaa aggcaatgag ttcttctgct acgacctgtc
1621 acacaacccc atccagagca gcactgatga gatcacactg gccttccgca ccctgcaacg
1681 caacggcctg atgctgcata caggcaagtc ggccgactac gtcaacctgt ccctcaagtc
1741 tggggctgtc tggctggtca tcaacctagg ctcaggtgcc ttcgaggccc ttgtggaacc
1801 cgtcaatggc aagttcaacg acaacgcctg gcacgacgtc cgggtcaccc gaaacctgcg
1861 ccagcacgca gggattggac acgctatggt aaacaaactg cattatctgg tgaccatctc
1921 ggtggacggg atcctgacca ccacaggcta cacgcaggag gattacacca tgctgggctc
1981 tgatgacttc ttctacattg ggggcagccc caacacagct gacctgccgg gctcgcccgt
2041 cagcaacaac ttcatgggct gcctcaagga cgtggtctat aagaacaatg acttcaaatt
2101 ggaactatcc cgcctggcaa aggaagggga ccccaagatg aagctgcagg gggacttgtc
2161 attccgctgt gaggatgtgg ctgccctgga ccctgtgacc tttgagagtc ccgaggcctt
2221 tgtggcgctg ccccgctgga gcgctaagcg cactggctcc atctccctag acttccgcac
2281 caccgagccc aatgggctgc tgctcttcag ccagggccgg cgggctgggg gtggagctgg
2341 cagccacagc tctgctcagc gggccgacta ctttgccatg gagctattgg acggccacct
2401 ctatcttctg ctggacatgg gatctggggg catcaagctg cgggcatcca gccgcaaggt
2461 caatgatggc gagtggtgtc acgtggactt ccagagggat gggcgaaaag gctccatctc
2521 agtgaatagt cgcagcacgc cgttcttggc cactggagac agcgagattc tggacctgga
2581 gagtgagctg tacctgggcg gtctccctga ggggggccgg gtggacctgc ccctgccccc
2641 agaggtgtgg acagcagcac tccgggcagg ctacgtgggc tgtgtgcggg acctcttcat
2701 agatgggcgt agccgagacc tccggggcct ggctgaggct cagggggctg tgggcgttgc
2761 ccccttttgc tcccgggaga cgctgaagca gtgtgcatct gccccctgtc gcaatggggg
2821 cgtctgtcga gaaggctgga accgcttcat ctgtgactgc atcgggaccg gctttcttgg
2881 gcgggtctgt gagagagagg ccacggtcct gagctacgat ggctccatgt acatgaagat
2941 catgctgcct aacgccatgc acacggaggc agaggatgtg tccctgcgtt tcatgtccca
3001 gcgggcctac ggactcatga tggccaccac ttccagggag tctgccgaca ccctacgcct
3061 ggagctggat ggggggcaga tgaagctcac tgtcaacctc gactgcctgc gcgtcggctg
3121 cgcacccagt aaaggccccg aaacgctgtt tgcggggcac aagctcaatg acaatgagtg
3181 gcacacggtg agggtggtcc ggcgtggcaa gagcctgcag ctgtctgtgg acaacgtgac
3241 tgtggaggga cagatggcag gagcccatat gcggctggag ttccacaaca ttgagacggg
3301 catcatgacg gagcggcggt ttatctccgt ggtgccctcc aacttcatcg ggcatctgag
3361 tgggctcgtg ttcaatggcc agccctacat ggaccagtgc aaggatggtg acatcaccta
3421 ctgtgagctc aatgctcgct ttggcctgcg tgccatcgtg gccgatcccg tcaccttcaa
3481 gagtcgcagc agctacctgg cactcgccac gctccaagcc tatgcttcca tgcacctctt
3541 cttccagttc aagaccacgg cccctgatgg gcttcttctg ttcaactcgg gcaacggcaa
3601 tgacttcatt gtcatcgagc tggtcaaggt aagtatcaag gttacaagac aggtttaagg
3661 agaccaataa gctattattc gtataggata ctttatacga agttattaag ttatggagac
3721 cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
3781 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
3841 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
3901 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
3961 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
4021 gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct
4081 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
4141 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
4201 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
4261 tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga
4321 tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa
4381 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
4441 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
4501 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
4561 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
4621 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
4681 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
4741 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
4801 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
4861 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
4921 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
4981 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
5041 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
5101 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
5161 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
5221 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
5281 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
5341 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
5401 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
5461 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
5521 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
5581 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
5641 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
5701 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
5761 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
5821 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
5881 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
5941 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
6001 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
6061 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
6121 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
6181 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
6241 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
6301 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
6361 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
6421 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
6481 tcacatgt
//
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