pUBC-mNeonGreen-1POK

LOCUS       pUBC-mNeonGreen-1POK           6345 bp DNA     circular SYN 05-APR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Ruizhao Wang
  TITLE     Direct Submission
  JOURNAL   Exported Jul 13, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     55..103
                     /label=pUBC-mChilada-f
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             83..790
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     primer_bind     complement(609..649)
                     /label=mNeonGreen_G183T-R
     primer_bind     632..656
                     /label=Vector_mNeonGreen-F
     primer_bind     complement(770..817)
                     /label=HA-mNeonGreen-R
     primer_bind     791..837
                     /label=HA-1POK-F
     CDS             791..817
                     /codon_start=1
                     /label=HA tag
                     /translation="YPYDVPDYA"
     CDS             818..853
                     /codon_start=1
                     /label=Linker12
                     /translation="GGSGGTGGSGGT"
     CDS             854..2020
                     /codon_start=1
                     /label=1P0K(E239Y)
                     /translation="IDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPSDI
                     VPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVGLLG
                     TDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIGVKCA
                     ISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLENCDVPI
                     SKLLPTHVNRNVPLFYQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIPLARVTL
                     SSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTSSVAGFLN
                     LTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     primer_bind     complement(1997..2044)
                     /label=NotI-1POK-R
     polyA_signal    2174..2229
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2590..2606)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2614..2630
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2638..2668)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     2643..2664
                     /label=lac-pro-F
     protein_bind    2683..2704
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2763..2958
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2788..2958
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2809..2944
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2964..3098
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3337..3925)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3341..3360)
                     /label=pUBC-primer-1
     CDS             complement(4096..4956)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4127..4148)
                     /label=Amp-F
     primer_bind     4870..4890
                     /label=Amp-R-seq
     promoter        complement(4957..5060)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5133..6345
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(5260..5282)
                     /label=hUBC-pro-R
     primer_bind     5728..5751
                     /label=pUBC-seq-2
     primer_bind     6182..6201
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 gaagcttggg ctgcaggtcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc
       61 ccgggatcca ccggtcgcca ccatggtgag caagggcgag gaggataaca tggcctctct
      121 cccagcgaca catgagttac acatctttgg ctccatcaac ggtgtggact ttgacatggt
      181 gggtcagggc accggcaatc caaatgatgg ttatgaggag ttaaacctga agtccaccaa
      241 gggtgacctc cagttctccc cctggattct ggtccctcat atcgggtatg gcttccatca
      301 gtacctgccc taccctgacg ggatgtcgcc tttccaggcc gccatggtag atggctccgg
      361 ataccaagtc catcgcacaa tgcagtttga agatggtgcc tcccttactg ttaactaccg
      421 ctacacctac gagggaagcc acatcaaagg agaggcccag gtgaagggga ctggtttccc
      481 tgctgacggt cctgtgatga ccaactcgct gaccgctgcg gactggtgca ggtcgaagaa
      541 gacttacccc aacgacaaaa ccatcatcag tacctttaag tggagttaca ccacgggaaa
      601 tggcaagcgc taccggagca ctgcgcggac cacctacacc tttgccaagc caatggcggc
      661 taactatctg aagaaccagc cgatgtacgt gttccgtaag acggagctca agcactccaa
      721 gaccgagctc aacttcaagg agtggcaaaa ggcctttacc gatgtgatgg gcatggacga
      781 gctgtacaag tatccatacg acgtgcctga ttatgcaggc ggcagcggcg gcaccggcgg
      841 cagcggcggc accatcgact acaccgccgc cggcttcacc ctgctgcagg gcgcccacct
      901 gtacgccccc gaggacaggg gcatctgcga cgtgctggtg gccaacggca agatcatcgc
      961 cgtggccagc aacatcccca gcgacatcgt gcccaactgc accgtggtgg acctgagcgg
     1021 ccagatcctg tgccccggct tcatcgacca gcacgtgcac ctgatcggcg gcggcggcga
     1081 ggccggcccc accaccagga cccccgaggt ggccctgagc aggctgaccg aggccggcgt
     1141 gaccagcgtg gtgggcctgc tgggcaccga cagcatcagc aggcaccccg agagcctgct
     1201 ggccaagacc agggccctga acgaggaggg catcagcgcc tggatgctga ccggcgccta
     1261 ccacgtgccc agcaggacca tcaccggcag cgtggagaag gacgtggcca tcatcgacag
     1321 ggtgatcggc gtgaagtgcg ccatcagcga ccacaggagc gccgcccccg acgtgtacca
     1381 cctggccaac atggccgccg agagcagggt gggcggcctg ctgggcggca agcccggcgt
     1441 gaccgtgttc cacatgggcg acagcaagaa ggccctgcag cccatctacg acctgctgga
     1501 gaactgcgac gtgcccatca gcaagctgct gcccacccac gtgaacagga acgtgcccct
     1561 gttctaccag gccctggagt tcgccaggaa gggcggcacc atcgacatca ccagcagcat
     1621 cgacgagccc gtggcccccg ccgagggcat cgccagggcc gtgcaggccg gcatccccct
     1681 ggccagggtg accctgagca gcgacggcaa cggcagccag cccttcttcg acgacgaggg
     1741 caacctgacc cacatcggcg tggccggctt cgagaccctg ctggagaccg tgcaggtgct
     1801 ggtgaaggac tacgacttca gcatcagcga cgccctgagg cccctgacca gcagcgtggc
     1861 cggcttcctg aacctgaccg gcaagggcga gatcctgccc ggcaacgacg ccgacctgct
     1921 ggtgatgacc cccgagctga ggatcgagca ggtgtacgcc aggggcaagc tgatggtgaa
     1981 ggacggcaag gcctgcgtga agggcacctt cgagacggcc tgagcggccg cactcctcag
     2041 gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac
     2101 cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
     2161 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
     2221 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
     2281 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta
     2341 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
     2401 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
     2461 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
     2521 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc
     2581 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     2641 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     2701 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat
     2761 ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     2821 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     2881 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     2941 ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa
     3001 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3061 tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca
     3121 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     3181 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3241 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3301 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3361 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3421 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3481 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3541 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3601 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3661 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3721 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3781 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3841 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3901 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3961 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4021 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4081 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4141 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4201 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4261 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4321 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4381 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4441 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4501 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4561 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4621 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4681 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     4741 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     4801 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4861 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4921 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc atattattga
     4981 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     5041 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc gacattgatt
     5101 attgactagt aacccgtgtc ggctccagat ctggcctccg cgccgggttt tggcgcctcc
     5161 cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg
     5221 tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt
     5281 agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact
     5341 ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc
     5401 ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc
     5461 acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga
     5521 tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg
     5581 ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca
     5641 aggttgccct gaactggggg ttggggggga gcgcagcaaa atggcggctg ttcccgagtc
     5701 ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg
     5761 gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg
     5821 gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga
     5881 actcggtttg tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc
     5941 cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat
     6001 aatgcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg
     6061 cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg
     6121 gagggataag tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag
     6181 ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt
     6241 taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt
     6301 aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagac
//