| 质粒名称: | mNeonGreen-lifeactin |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000301 |
| 产品用途: | Expresses mNeonGreen-LifeAct in mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a, TOP12 |
|
插入基因信息 |
|
| 插入基因名称: | mNeonGreen, LifeAct |
| 物种来源: | Synthetic, synthetic |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Bright and stable monomeric green fluorescent protein derived from StayGold
Zhang H., Lesnov G.D., Subach O.M., Zhang W., Kuzmicheva T.P., Vlaskina A.V., Samygina V.R., Chen L., Ye X., Nikolaeva A.Y., Gabdulkhakov A., Papadaki S., Qin W., Borshchevskiy V., Perfilov M.M., Gavrikov A.S., Drobizhev M., Mishin A.S., Piatkevich K.D., Subach F.V.
Nat. Methods 2024 214 21, 657–665 (2024)
|
mNeonGreen-lifeactin
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
mNeonGreen-lifeactin载体质粒基本信息
mNeonGreen-lifeactin质粒图谱载体图谱和mNeonGreen-lifeactin载体序列质粒序列多克隆位点信息
mNeonGreen-lifeactin质粒载体简介
mNeonGreen-lifeactin质粒序列载体序列
LOCUS mNeonGreen-lifeactin 4726 bp DNA circular SYN 01-NOV-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4726)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4726
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 1..33
/label=Mutation
/label=nonstandard type: YSP
misc_feature 1..33
/label=Extracted region from Y54-C1 pDR2x-mVenus(L46F;
T63S)-P2A-PAmCherry-T2A-mtagBFP1
/label=nonstandard type: extracted
misc_feature 1..33
/label=Bsp1407I
/label=nonstandard type: restriction
misc_feature 1..17
/label=纯去除mito后
primer_bind 17..53
/label=mNG-F
primer_bind 17..38
/label=42-F
primer_bind 17..37
/label=49-F
primer_bind complement(17..33)
/label=mgold载体反向
primer_bind 28..53
/label=0719-PM948-F
regulatory 28..33
/label=vertebrate consensus sequence for strong initia
/label=vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)
CDS 34..741
/codon_start=1
/product="bright monomeric yellow-green fluorescent protein
derived from LanYFP (Shaner et al., 2013)"
/label=mNeonGreen
/translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
KAFTDVMGMDELYK"
regulatory 34..37
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
regulatory 289..298
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
primer_bind complement(721..756)
/label=mNG-R
primer_bind complement(721..741)
/label=0719-PM948-R
primer_bind complement(725..756)
/label=42-R
primer_bind 742..763
/label=mgold载体正向
primer_bind complement(742..756)
/label=OXstaygold-R
CDS 742..744
/label=mGold CDS
CDS 742..744
/label=mVenus
CDS 742..744
/label=Translation 1435-1437
polyA_signal 867..988
/label=SV40 poly(A) signal
primer_bind complement(904..923)
/label=SV40pA-R
primer_bind 958..977
/label=EBV-rev
primer_bind complement(965..988)
/label=SV40-POLYA-R
rep_origin complement(995..1450)
/direction=LEFT
/label=f1 ori
primer_bind complement(1132..1153)
/label=F1ori-F
primer_bind 1344..1363
/label=F1ori-R
promoter 1477..1581
/label=AmpR promoter
promoter 1583..1940
/label=SV40 promoter
primer_bind complement(1606..1626)
/label=pBABE 3'
rep_origin 1791..1926
/label=SV40 ori
primer_bind 1853..1872
/label=SV40pro-F
CDS 1975..2769
/label=NeoR/KanR
primer_bind complement(2029..2048)
/label=Neo-R
primer_bind 2639..2658
/label=Neo-F
primer_bind 2750..2771
/label=KanR-F
primer_bind complement(2750..2771)
/label=KanR-R
primer_bind complement(2957..2976)
/label=TK-pA-R
polyA_signal 3001..3048
/label=HSV TK poly(A) signal
rep_origin 3377..3965
/label=ori
primer_bind 3866..3885
/label=pBR322ori-F
misc_feature 4067..4675
/label=Mutation
/label=nonstandard type: YSP
misc_feature 4067..4675
/label=YSP021 T63S mutation
/label=nonstandard type: YSP
misc_feature 4067..4675
/label=YSP10,11
/label=nonstandard type: YSP
enhancer 4140..4443
/label=CMV enhancer
promoter 4444..4647
/label=CMV promoter
primer_bind 4598..4618
/label=CMV-F
primer_bind 4598..4618
/label=CMV-F
primer_bind complement(4658..4675)
/label=载体.反向
misc_feature 4659..4675
/label=mito前
primer_bind 4661..4690
/label=片段.正向
CDS 4676..4726
/codon_start=1
/product="peptide that binds filamentous actin (Riedl et
al., 2008)"
/label=Lifeact
/note="first 17 amino acids of budding yeast Abp140"
/translation="MGVADLIKKFESISKEE"
ORIGIN
1 atccattcgt tgggggatcc accggtcgcc accatggtga gcaagggcga ggaggataac
61 atggcctctc tcccagcgac acatgagtta cacatctttg gctccatcaa cggtgtggac
121 tttgacatgg tgggtcaggg caccggcaat ccaaatgatg gttatgagga gttaaacctg
181 aagtccacca agggtgacct ccagttctcc ccctggattc tggtccctca tatcgggtat
241 ggcttccatc agtacctgcc ctaccctgac gggatgtcgc ctttccaggc cgccatggta
301 gatggctccg gctaccaagt ccatcgcaca atgcagtttg aagatggtgc ctcccttact
361 gttaactacc gctacaccta cgagggaagc cacatcaaag gagaggccca ggtgaagggg
421 actggtttcc ctgctgacgg tcctgtgatg accaactcgc tgaccgctgc ggactggtgc
481 aggtcgaaga agacttaccc caacgacaaa accatcatca gtacctttaa gtggagttac
541 accacgggaa atggcaagcg ctaccggagc actgcgcgga ccacctacac ctttgccaag
601 ccaatggcgg ctaactatct gaagaaccag ccgatgtacg tgttccgtaa gacggagctc
661 aagcactcca agaccgagct caacttcaag gagtggcaaa aggcctttac cgatgtgatg
721 ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca
781 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
841 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
901 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
961 gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta
1021 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
1081 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
1141 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
1201 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
1261 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
1321 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
1381 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
1441 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
1501 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
1561 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
1621 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
1681 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
1741 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
1801 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
1861 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
1921 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
1981 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
2041 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
2101 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
2161 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
2221 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
2281 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
2341 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
2401 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
2461 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
2521 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
2581 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
2641 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
2701 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
2761 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
2821 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
2881 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
2941 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
3001 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
3061 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
3121 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
3181 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
3241 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
3301 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
3361 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
3421 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
3481 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
3541 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
3601 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
3661 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
3721 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
3781 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
3841 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
3901 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
3961 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
4021 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt
4081 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
4141 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
4201 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
4261 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
4321 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
4381 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
4441 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
4501 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
4561 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
4621 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcatggg
4681 cgtggccgac ctgatcaaga agttcgagag catcagcaag gaggag
//
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