PCDH-TetO-mEGFP-TRIM41-TurboID

LOCUS       PCDH-TetO-mEGFP-TRIM41-TurboID              10067 bp DNA     circular SYN 04-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10067)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1980..2250
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2283..2320
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2444..3160
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      2444..2447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3161..3190
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    3197..5086
                     /label=41
     CDS             5093..5119
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5123..5149
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5153..5179
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5180..6160
                     /codon_start=1
                     /label=TurboID
                     /translation="ASKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKS
                     GDACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEKEFSRAD"
     misc_feature    6164..6751
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6635..6646)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6824..7057
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7129..7250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7269..7404
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7442..7458)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7466..7482
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7490..7520)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7535..7556
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7844..8432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8603..9463)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9464..9568)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10042..10058
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtcgg gtttattaca gggacagcag agatccagtt tggttatcga gtttaccact
     1981 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2041 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     2101 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     2161 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2221 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc
     2281 gagtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat
     2341 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     2401 cctccgcggc ccctctagac cgctagcgct accggtcgcc accatggtga gcaagggcga
     2461 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     2521 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     2581 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     2641 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     2701 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     2761 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     2821 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     2881 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     2941 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3001 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3061 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3121 cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagc
     3181 tcaagcttcg gaattcatgg ctgccgttgc catgacaccc aaccctgtgc agacccttca
     3241 ggaggaggcg gtgtgcgcca tctgcctcga ttacttcacg gaccccgtgt ccatcggctg
     3301 cgggcacaac ttctgccgag tttgtgtaac ccagttgtgg ggtggggagg atgaggagga
     3361 cagagatgag ttagatcggg aggaggagga ggaggacgga gaggaggagg aagtggaggc
     3421 tgtgggggct ggcgcggggt gggacacccc catgcgggat gaagactacg agggtgacat
     3481 ggaggaggag gtcgaggagg aagaagaggg tgtgttctgg accagtggca tgagcaggtc
     3541 cagctgggac aacatggact atgtgtggga ggaggaggac gaggaggaag acctggacta
     3601 ctacttgggg gacatggagg aggaggacct gaggggggag gatgaggagg acgaggagga
     3661 agtgctggag gaggttgagg aagaggatct agaccccgtc accccactgc ccccgcctcc
     3721 agcccctcgg aggtgcttca catgccctca gtgccgaaag agctttcctc ggcggagctt
     3781 ccgccccaac ctgcagctgg ccaatatggt ccaggtgatt cggcagatgc acccaacccc
     3841 tggtcgaggg agccgcgtga ccgatcaggg catctgtccc aaacaccaag aagccctgaa
     3901 gctcttctgc gaggtagacg aagaggccat ctgtgtggtg tgccgagaat ccaggagcca
     3961 caaacagcac agcgtggtgc cattggagga ggtggtgcag gagtacaagg ccaaactgca
     4021 ggggcacgtg gaaccactga ggaagcacct ggaggcagtg cagaagatga aagccaagga
     4081 ggagaggcga gtgacagaac tgaagagcca gatgaagtca gagctggcag cggtggcctc
     4141 ggagtttggg cgactgacac ggtttctggc tgaagagcag gcagggctgg aacggcgtct
     4201 cagagagatg catgaagccc agctggggcg tgcgggagcc gcggctagtc gccttgcaga
     4261 acaggccgcc cagctcagcc gcctgctggc agaggcccag gagcggagcc agcagggggg
     4321 tctccggctg ctccaggaca tcaaggagac tttcaatagg tgtgaagagg tacagctgca
     4381 gcccccagag gtctggtccc ctgacccgtg ccaaccccat agccatgact tcctgacaga
     4441 tgccatcgtg aggaaaatga gccggatgtt ctgtcaggct gcgagagtgg acctgacgct
     4501 ggaccctgac acggctcacc cggccctgat gctgtcccct gaccgccggg gggtccgcct
     4561 ggcagagcgg cggcaggagg ttgctgacca tcccaagcgc ttctcggccg actgctgcgt
     4621 actgggggcc cagggcttcc gctccggccg gcactactgg gaggtagagg tgggcgggcg
     4681 gcggggctgg gcggtgggtg ctgcccgtga atcaacccat cataaggaaa aggtgggccc
     4741 tgggggttcc tccgtgggca gcggggatgc cagctcctcg cgccatcacc atcgccgccg
     4801 ccggctccac ctgccccagc agcccctgct ccagcgggaa gtgtggtgcg tgggcaccaa
     4861 cggcaaacgc tatcaggccc agagctccac agaacagacg ctgctgagcc ccagtgagaa
     4921 accaaggcgc tttggtgtgt acctggacta tgaagctggg cgcctgggct tctacaacgc
     4981 agagactcta gcccacgtgc acaccttctc ggctgccttc ctgggcgagc gtgtctttcc
     5041 tttcttccgg gtgctctcca agggcacccg catcaagctc tgccctggat cctacccgta
     5101 tgatgttccg gattacgctg gctatcccta cgacgtgccc gactatgccg ggtaccccta
     5161 tgacgtccca gactacgcag ctagcaaaga caatactgtg cctctgaagc tgatcgctct
     5221 cctggctaat ggcgagttcc atagtggcga acagctggga gaaaccctgg gcatgtccag
     5281 ggccgctatc aacaagcaca ttcagactct gcgcgactgg ggcgtggacg tgttcaccgt
     5341 gcccggaaag ggctactctc tgcccgagcc tatcccgctg ctgaacgcta aacagattct
     5401 gggacagctg gacggcggga gcgtggcagt cctgcctgtg gtcgactcca ccaatcagta
     5461 cctgctggat cgaatcggcg agctgaagag tggggatgct tgcattgcag aatatcagca
     5521 ggcagggaga ggaagcagag ggaggaaatg gttctctcct tttggagcta acctgtacct
     5581 gagtatgttt tggcgcctga agcggggacc agcagcaatc ggcctgggcc cggtcatcgg
     5641 aattgtcatg gcagaagcgc tgcgaaagct gggagcagac aaggtgcgag tcaaatggcc
     5701 caatgacctg tatctgcagg atagaaagct ggcaggcatc ctggtggagc tggccggaat
     5761 aacaggcgat gctgcacaga tcgtcattgg cgccgggatt aacgtggcta tgaggcgcgt
     5821 ggaggaaagc gtggtcaatc agggctggat cacactgcag gaagcaggga ttaacctgga
     5881 caggaatact ctggccgcta cgctgatccg agagctgcgg gcagccctgg aactgttcga
     5941 gcaggaaggc ctggctccat atctgccacg gtgggagaag ctggataact tcatcaatag
     6001 acccgtgaag ctgatcattg gggacaaaga gattttcggg attagccggg ggattgataa
     6061 acagggagcc ctgctgctgg aacaggacgg agttatcaaa ccctggatgg gcggagaaat
     6121 cagtctgcgg tctgccgaaa aggagttcag cagggccgac tgaaatcaac ctctggatta
     6181 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     6241 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     6301 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     6361 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     6421 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     6481 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     6541 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     6601 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     6661 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt ctccgccttc gccctcagac
     6721 gagtcggatc tccctttggc cgcctccccg ccggtacctt taagaccaat gacttacaag
     6781 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     6841 tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     6901 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6961 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7021 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     7081 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     7141 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7201 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     7261 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7321 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     7381 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat
     7441 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7501 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7681 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7741 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7801 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7861 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7921 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7981 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8041 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8101 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8161 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8221 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8281 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8341 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8401 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8461 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8521 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8581 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8641 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8701 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8761 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8821 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8881 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8941 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9001 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9061 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9121 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9181 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9241 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9301 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9361 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9421 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9481 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9541 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     9601 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     9661 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     9721 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     9781 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     9841 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     9901 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     9961 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
    10021 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//