PCDH-TetO-mEGFP-TRIM38-TurboID

LOCUS       PCDH-TetO-mEGFP-TRIM38-TurboID             9572 bp DNA     circular SYN 04-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9572)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1980..2250
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2283..2320
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2444..3160
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      2444..2447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3161..3190
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    3197..4591
                     /label=TRIM38
     CDS             4598..4624
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4628..4654
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4658..4684
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4685..5665
                     /codon_start=1
                     /label=TurboID
                     /translation="ASKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKS
                     GDACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEKEFSRAD"
     misc_feature    5669..6256
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6140..6151)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6329..6562
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6634..6755
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6774..6909
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6947..6963)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6971..6987
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6995..7025)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7040..7061
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7349..7937)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8108..8968)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8969..9073)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9547..9563
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtcgg gtttattaca gggacagcag agatccagtt tggttatcga gtttaccact
     1981 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2041 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     2101 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     2161 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2221 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc
     2281 gagtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat
     2341 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     2401 cctccgcggc ccctctagac cgctagcgct accggtcgcc accatggtga gcaagggcga
     2461 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     2521 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     2581 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     2641 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     2701 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     2761 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     2821 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     2881 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     2941 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3001 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3061 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3121 cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagc
     3181 tcaagcttcg gaattcatgg cctcaaccac cagcaccaag aagatgatgg aggaagccac
     3241 ctgctccatc tgcctgagcc tgatgacgaa cccagtaagc atcaactgtg gacacagcta
     3301 ctgccacttg tgtataacag acttctttaa aaacccaagc caaaagcaac tgaggcagga
     3361 gacattctgc tgtccccagt gtcgggctcc atttcatatg gatagcctcc gacccaacaa
     3421 gcagctggga agcctcattg aagccctcaa agagacggat caagaaatgt catgtgagga
     3481 acacggagag cagttccacc tgttctgcga agacgagggg cagctcatct gctggcgctg
     3541 tgagcgggca ccacagcaca aagggcacac cacagctctt gttgaagacg tatgccaggg
     3601 ctacaaggaa aagctccaga aagctgtgac aaaactgaag caacttgaag acagatgtac
     3661 ggagcagaag ctgtccacag caatgcgaat aactaaatgg aaagagaagg tacagattca
     3721 gagacaaaaa atccggtctg actttaagaa tctccagtgt ttcctacatg aggaagagaa
     3781 gtcttatctc tggaggctgg agaaagaaga acaacagact ctgagtagac tgagggacta
     3841 tgaggctggt ctggggctga agagcaatga actcaagagc cacatcctgg aactggagga
     3901 aaaatgtcag ggctcagccc agaaattgct gcagaatgtg aatgacactt tgagcaggag
     3961 ttgggctgtg aagctggaaa catcagaggc tgtctccttg gaacttcata ctatgtgcaa
     4021 tgtttccaag ctttacttcg atgtgaagaa aatgttaagg agtcatcaag ttagtgtgac
     4081 tctggatcca gatacagctc atcacgaact aattctctct gaggatcgga gacaagtgac
     4141 tcgtggatac acccaggaga atcaggacac atcttccagg agatttactg ccttcccctg
     4201 tgtcttgggt tgtgaaggct tcacctcagg aagacgttac tttgaagtgg atgttggcga
     4261 aggaaccgga tgggatttag gagtttgtat ggaaaatgtg cagaggggca ctggcatgaa
     4321 gcaagagcct cagtctggat tctggaccct caggctgtgc aaaaagaaag gctatgtagc
     4381 acttacttct cccccaactt cccttcatct gcatgagcag cccctgcttg tgggaatttt
     4441 tctggactat gaggccggag ttgtatcctt ttataacggg aatactggct gccacatctt
     4501 tactttcccg aaggcttcct tctctgatac tctccggccc tatttccagg tttatcaata
     4561 ttctcctttg tttctgcctc ccccaggtga cggatcctac ccgtatgatg ttccggatta
     4621 cgctggctat ccctacgacg tgcccgacta tgccgggtac ccctatgacg tcccagacta
     4681 cgcagctagc aaagacaata ctgtgcctct gaagctgatc gctctcctgg ctaatggcga
     4741 gttccatagt ggcgaacagc tgggagaaac cctgggcatg tccagggccg ctatcaacaa
     4801 gcacattcag actctgcgcg actggggcgt ggacgtgttc accgtgcccg gaaagggcta
     4861 ctctctgccc gagcctatcc cgctgctgaa cgctaaacag attctgggac agctggacgg
     4921 cgggagcgtg gcagtcctgc ctgtggtcga ctccaccaat cagtacctgc tggatcgaat
     4981 cggcgagctg aagagtgggg atgcttgcat tgcagaatat cagcaggcag ggagaggaag
     5041 cagagggagg aaatggttct ctccttttgg agctaacctg tacctgagta tgttttggcg
     5101 cctgaagcgg ggaccagcag caatcggcct gggcccggtc atcggaattg tcatggcaga
     5161 agcgctgcga aagctgggag cagacaaggt gcgagtcaaa tggcccaatg acctgtatct
     5221 gcaggataga aagctggcag gcatcctggt ggagctggcc ggaataacag gcgatgctgc
     5281 acagatcgtc attggcgccg ggattaacgt ggctatgagg cgcgtggagg aaagcgtggt
     5341 caatcagggc tggatcacac tgcaggaagc agggattaac ctggacagga atactctggc
     5401 cgctacgctg atccgagagc tgcgggcagc cctggaactg ttcgagcagg aaggcctggc
     5461 tccatatctg ccacggtggg agaagctgga taacttcatc aatagacccg tgaagctgat
     5521 cattggggac aaagagattt tcgggattag ccgggggatt gataaacagg gagccctgct
     5581 gctggaacag gacggagtta tcaaaccctg gatgggcgga gaaatcagtc tgcggtctgc
     5641 cgaaaaggag ttcagcaggg ccgactgaaa tcaacctctg gattacaaaa tttgtgaaag
     5701 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     5761 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     5821 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     5881 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     5941 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6001 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6061 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6121 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6181 gccggctctg cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct
     6241 ttggccgcct ccccgccggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     6301 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa
     6361 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6421 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6481 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6541 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     6601 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     6661 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6721 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     6781 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6841 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     6901 ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc
     6961 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7021 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7081 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7141 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7201 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7261 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7321 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7381 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7441 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7501 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7561 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7621 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7681 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7741 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     7801 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7861 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7921 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7981 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8041 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8101 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8161 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8221 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8281 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8341 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8401 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8461 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8521 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8581 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8641 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8701 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8761 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8821 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8881 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8941 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9001 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9061 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     9121 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     9181 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     9241 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     9301 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     9361 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     9421 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     9481 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     9541 gacgttgtaa aacgacggcc agtgccaagc tg
//