PCDH-TetO-mEGFP-TRIM34-TurboID

LOCUS       PCDH-TetO-mEGFP-TRIM34-TurboID               9653 bp DNA     circular SYN 04-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9653)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 12, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1980..2250
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2283..2320
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2444..3160
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      2444..2447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3161..3190
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    3197..4660
                     /label=TRIM34
     CDS             4679..4705
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4709..4735
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4739..4765
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4766..5746
                     /codon_start=1
                     /label=TurboID
                     /translation="ASKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKS
                     GDACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEKEFSRAD"
     misc_feature    5750..6337
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6221..6232)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6410..6643
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6715..6836
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6855..6990
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7028..7044)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7052..7068
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7076..7106)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7121..7142
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7430..8018)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8189..9049)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9050..9154)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9628..9644
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtcgg gtttattaca gggacagcag agatccagtt tggttatcga gtttaccact
     1981 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2041 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     2101 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     2161 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2221 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc
     2281 gagtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat
     2341 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     2401 cctccgcggc ccctctagac cgctagcgct accggtcgcc accatggtga gcaagggcga
     2461 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     2521 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     2581 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     2641 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     2701 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     2761 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     2821 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     2881 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     2941 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3001 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3061 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3121 cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagc
     3181 tcaagcttcg gaattcatgg cttcaaaaat cttgcttaac gtacaagagg aggtgacctg
     3241 tcccatctgc ctggagctgt tgacagaacc cttgagtcta gactgtggcc acagcctctg
     3301 ccgagcctgc atcactgtga gcaacaagga ggcagtgacc agcatgggag gaaaaagcag
     3361 ctgtcctgtg tgtggtatca gttactcatt tgaacatcta caggctaatc agcatctggc
     3421 caacatagtg gagagactca aggaggtcaa gttgagccca gacaatggga agaagagaga
     3481 tctctgtgat catcatggag agaaactcct actcttctgt aaggaggata ggaaagtcat
     3541 ttgctggctt tgtgagcggt ctcaggagca ccgtggtcac cacacagtcc tcacggagga
     3601 agtattcaag gaatgtcagg agaaactcca ggcagtcctc aagaggctga agaaggaaga
     3661 ggaggaagct gagaagctgg aagctgacat cagagaagag aaaacttcct ggaagtatca
     3721 ggtacaaact gagagacaaa ggatacaaac agaatttgat cagcttagaa gcatcctaaa
     3781 taatgaggag cagagagagc tgcaaagatt ggaagaagaa gaaaagaaga cgctggataa
     3841 gtttgcagag gctgaggatg agctagttca gcagaagcag ttggtgagag agctcatctc
     3901 agatgtggag tgtcggagtc agtggtcaac aatggagctg ctgcaggaca tgagtggaat
     3961 catgaaatgg agtgagatct ggaggctgaa aaagccaaaa atggtttcca agaaactgaa
     4021 gactgtattc catgctccag atctgagtag gatgctgcaa atgtttagag aactgacagc
     4081 tgtccggtgc tactgggtgg atgtcacact gaattcagtc aacctaaatt tgaatcttgt
     4141 cctttcagaa gatcagagac aagtgatatc tgtgccaatt tggccttttc agtgttataa
     4201 ttatggtgtc ttgggatccc aatatttctc ctctgggaaa cattactggg aagtggacgt
     4261 gtccaagaaa actgcctgga tcctgggggt atactgtaga acatattccc gccatatgaa
     4321 gtatgttgtt agaagatgtg caaatcgtca aaatctttac accaaataca gacctctatt
     4381 tggctactgg gttatagggt tacagaataa atgtaagtat ggtgtctttg aagagtcttt
     4441 gtcctctgat cccgaggttt tgactctctc catggctgtg cctccctgcc gtgttggggt
     4501 tttcctcgac tatgaagcag gcattgtctc atttttcaat gtcacaagcc atggctccct
     4561 catttacaag ttctctaaat gttgcttttc tcagcctgtt tatccatatt tcaatccttg
     4621 gaactgtcca gctcccatga ctctatgccc accaagctct ggatccggat ctggctccta
     4681 cccgtatgat gttccggatt acgctggcta tccctacgac gtgcccgact atgccgggta
     4741 cccctatgac gtcccagact acgcagctag caaagacaat actgtgcctc tgaagctgat
     4801 cgctctcctg gctaatggcg agttccatag tggcgaacag ctgggagaaa ccctgggcat
     4861 gtccagggcc gctatcaaca agcacattca gactctgcgc gactggggcg tggacgtgtt
     4921 caccgtgccc ggaaagggct actctctgcc cgagcctatc ccgctgctga acgctaaaca
     4981 gattctggga cagctggacg gcgggagcgt ggcagtcctg cctgtggtcg actccaccaa
     5041 tcagtacctg ctggatcgaa tcggcgagct gaagagtggg gatgcttgca ttgcagaata
     5101 tcagcaggca gggagaggaa gcagagggag gaaatggttc tctccttttg gagctaacct
     5161 gtacctgagt atgttttggc gcctgaagcg gggaccagca gcaatcggcc tgggcccggt
     5221 catcggaatt gtcatggcag aagcgctgcg aaagctggga gcagacaagg tgcgagtcaa
     5281 atggcccaat gacctgtatc tgcaggatag aaagctggca ggcatcctgg tggagctggc
     5341 cggaataaca ggcgatgctg cacagatcgt cattggcgcc gggattaacg tggctatgag
     5401 gcgcgtggag gaaagcgtgg tcaatcaggg ctggatcaca ctgcaggaag cagggattaa
     5461 cctggacagg aatactctgg ccgctacgct gatccgagag ctgcgggcag ccctggaact
     5521 gttcgagcag gaaggcctgg ctccatatct gccacggtgg gagaagctgg ataacttcat
     5581 caatagaccc gtgaagctga tcattgggga caaagagatt ttcgggatta gccgggggat
     5641 tgataaacag ggagccctgc tgctggaaca ggacggagtt atcaaaccct ggatgggcgg
     5701 agaaatcagt ctgcggtctg ccgaaaagga gttcagcagg gccgactgaa atcaacctct
     5761 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     5821 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     5881 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     5941 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6001 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6061 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6121 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     6181 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6241 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc gccttcgccc
     6301 tcagacgagt cggatctccc tttggccgcc tccccgccgg tacctttaag accaatgact
     6361 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     6421 attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac
     6481 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     6541 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     6601 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     6661 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     6721 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6781 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6841 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6901 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     6961 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt
     7021 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7081 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7141 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7201 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7261 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7321 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7381 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7441 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7501 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7561 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7621 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7681 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7741 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7801 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7861 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7921 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7981 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8041 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8101 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8161 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8221 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8281 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8341 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8401 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8461 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8521 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8581 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8641 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8701 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8761 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8821 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8881 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8941 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9001 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9061 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9121 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9181 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     9241 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     9301 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     9361 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     9421 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     9481 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     9541 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     9601 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//