pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF

LOCUS       pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1              10869 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1.
ACCESSION   .
VERSION     .
KEYWORDS    pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10869)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 10869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10869
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        12..297
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        299..576
                     /label=chicken beta-actin promoter
     intron          577..804
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      816..825
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3567..3587
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3588..3608
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3627..3653
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3681..6179
                     /codon_start=1
                     /product="C-terminal portion of catalytically inactive 
                     Streptococcus pyogenes dCas9 carrying the N863A mutation 
                     (Zetsche et al., 2015)"
                     /label=dCas9(C)
                     /translation="KPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEI
                     SGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTY
                     AHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIH
                     DDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPE
                     NIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYL
                     QNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQI
                     LDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
                     GTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
                     ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIL
                     PKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
                     RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALP
                     SKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLD
                     KVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATL
                     IHQSITGLYETRIDLSQLGGD"
     CDS             6183..6203
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6222..6371
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             6399..6452
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6468..6854
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             6909..6929
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6945..7487
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             7512..7883
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     primer_bind     complement(7911..7928)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7917..8124
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8133..8273
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   8133..8262
                     /label=AAV2 ITR
     rep_origin      8348..8803
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8435..8454)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8645..8666
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8820..8839)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8939..8961
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8999..9017)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9085..9189
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9190..10050
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9408..9427)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10221..10809
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10710..10729
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
       61 ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg
      121 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      181 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca gtacatgacc
      241 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      301 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      361 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
      421 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      481 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      541 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      601 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      661 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gctgagcaag
      721 aggtaagggt ttaagggatg gttggttggt ggggtattaa tgtttaatta cctggagcac
      781 ctgcctgaaa tcactttttt tcaggttgga ccggtgccac catggtcagc tactgggaca
      841 ccggggtcct gctgtgcgcg ctgctcagct gtctgcttct cacaggatct agttcaggtt
      901 caaaattaaa agatcctgaa ctgagtttaa aaggcaccca gcacatcatg caagcaggcc
      961 agacactgca tctccaatgc aggggggaag cagcccataa atggtctttg cctgaaatgg
     1021 tgagtaagga aagcgaaagg ctgagcataa ctaaatctgc ctgtggaaga aatggcaaac
     1081 aattctgcag tactttaacc ttgaacacag ctcaagcaaa ccacactggc ttctacagct
     1141 gcaaatatct agctgtacct acttcaaaga agaaggaaac agaatctgca atctatatat
     1201 ttattagtga tacaggtaga cctttcgtag agatgtacag tgaaatcccc gaaattatac
     1261 acatgactga aggaagggag ctcgtcattc cctgccgggt tacgtcacct aacatcactg
     1321 ttactttaaa aaagtttcca cttgacactt tgatccctga tggaaaacgc ataatctggg
     1381 acagtagaaa gggcttcatc atatcaaatg caacgtacaa agaaataggg cttctgacct
     1441 gtgaagcaac agtcaatggg catttgtata agacaaacta tctcacacat cgacaaacca
     1501 atacaatcat agatgtccaa ataagcacac cacgcccagt caaattactt agaggccata
     1561 ctcttgttct caattgtact gctaccactc ccttgaacac gagagttcaa atgacctgga
     1621 gttaccctga tgaaaaaaat aagagagctt ccgtaaggcg acgaattgac caaagcaatt
     1681 cccatgccaa catattctac agtgttctta ctattgacaa aatgcagaac aaagacaaag
     1741 gactttatac ttgtcgtgta aggagtggac catcattcaa atctgttaac acctcagtgc
     1801 atatatatga taaagcattc atcactgtga aacatcgaaa acagcaggtg cttgaaaccg
     1861 tagctggcaa gcggtcttac cggctctcta tgaaagtgaa ggcatttccc tcgccggaag
     1921 ttgtatggtt aaaagatggg ttacctgcga ctgagaaatc tgctcgctat ttgactcgtg
     1981 gctactcgtt aattatcaag gacgtaactg aagaggatgc agggaattat acaatcttgc
     2041 tgagcataaa acagtcaaat gtgtttaaaa acctcactgc cactctaatt gtcaatgtga
     2101 aaccccagat ttacgaaaag gctgtgtcat cgtttccaga cccggctctc tacccactgg
     2161 gcagcagaca aatcctgact tgtaccgcat atggtatccc tcaacctaca atcaagtggt
     2221 tctggcaccc ctgtaaccat aatcattccg aagcaaggtg tgacttttgt tccaataatg
     2281 aagagtcctt tatcctggat gctgacagca acatgggaaa cagaattgag agcatcactc
     2341 agcgcatggc aataatagaa ggaaagaata agatggctag caccttggtt gtggctgact
     2401 ctagaatttc tggaatctac atttgcatag cttccaataa agttgggact gtgggaagaa
     2461 acataagctt ttatatcaca gatgtgccaa atgggtttca tgttaacttg gaaaaaatgc
     2521 cgacggaagg agaggacctg aaactgtctt gcacagttaa caagttctta tacagagacg
     2581 ttacttggat tttactgcgg acagttaata acagaacaat gcactacagt attagcaagc
     2641 aaaaaatggc catcactaag gagcactcca tcactcttaa tcttaccatc atgaatgttt
     2701 ccctgcaaga ttcaggcacc tatgcctgca gagccaggaa tgtatacaca ggggaagaaa
     2761 tcctccagaa gaaagaaatt acaatcagag atcaggaagc accatacctc ctgcgaaacc
     2821 tcagtgatca cacagtggcc atcagcagtt ccaccacttt agactgtcat gctaatggtg
     2881 tccccgagcc tcagatcact tggtttaaaa acaaccacaa aatacaacaa gagcctggaa
     2941 ttattttagg accaggaagc agcacgctgt ttattgaaag agtcacagaa gaggatgaag
     3001 gtgtctatca ctgcaaagcc accaaccaga agggctctgt ggaaagttca gcatacctca
     3061 ctgttcaagg aacctcggac aagcctaatc tggagctgat cactctaaca tgcacctgtg
     3121 tggctgcgac tctcttctgg ctcctattaa ccctctttat cggcggtggc tcgggtggtg
     3181 gctcgaagag catgtctagc atggtgtcag acactagttg cacattccct tcatctgatg
     3241 gcatattctg gaagcattgg attcaaacca aggatgggca gtgtggcagt ccattagtat
     3301 caactagaga tgggttcatt gttggtatac actcagcatc gaatttcacc aacacaaaca
     3361 attatttcac aagcgtgccg aaaaacttca tggaattgtt gacaaatcag gaggcgcagc
     3421 agtgggttag tggttggcga ttaaatgctg actcagtatt gtgggggggc cataaagttt
     3481 tcatggtgaa acctgaagag ccttttcagc cagttaagga agcgactcaa ctcatgaatc
     3541 gtcgtcgccg tcccggcggt ggctcggaaa acctgtattt tcaggggccc aagaagaaga
     3601 ggaaggtggg gggaggcagc actagttacc catacgacgt accagattac gctggaggtt
     3661 ctggatccgg cggtggctcg aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg
     3721 tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact
     3781 tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg
     3841 cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca
     3901 atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca
     3961 gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga
     4021 agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg
     4081 gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg
     4141 ccaacagaaa cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc
     4201 agaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg
     4261 gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga
     4321 aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga
     4381 ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca
     4441 aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg
     4501 agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg
     4561 acatcaaccg gctgtccgac tacgatgtgg accatatcgt gcctcagagc tttctgaagg
     4621 acgactccat cgacaacaag gtgctgacca gaagcgacaa ggcccggggc aagagcgaca
     4681 acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg
     4741 ccaagctgat tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga
     4801 gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa
     4861 agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc
     4921 tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg
     4981 atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc
     5041 tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg
     5101 tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa
     5161 tcggcaaggc taccgccaag tacttcttct acagcaacat catgaacttt ttcaagaccg
     5221 agattaccct ggccaacggc gagatccgga agcggcctct gatcgagaca aacggcgaaa
     5281 ccggggagat cgtgtgggat aagggccggg attttgccac cgtgcggaaa gtgctgagca
     5341 tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc agcaaagagt
     5401 ctatcctgcc caagaggaac agcgataagc tgatcgccag aaagaaggac tgggacccta
     5461 agaagtacgg cggcttcgac agccccaccg tggcctattc tgtgctggtg gtggccaaag
     5521 tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctgctgggg atcaccatca
     5581 tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag ggctacaaag
     5641 aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag ctggaaaacg
     5701 gccggaagag aatgctggcc tctgccggcg aactgcagaa gggaaacgaa ctggccctgc
     5761 cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg aagggctccc
     5821 ccgaggataa tgagcagaaa cagctgtttg tggaacagca caagcactac ctggacgaga
     5881 tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct aatctggaca
     5941 aagtgctgtc cgcctacaac aagcaccggg ataagcccat cagagagcag gccgagaata
     6001 tcatccacct gtttaccctg accaatctgg gagcccctgc cgccttcaag tactttgaca
     6061 ccaccatcga ccggaagagg tacaccagca ccaaagaggt gctggacgcc accctgatcc
     6121 accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg ggaggcgaca
     6181 gccccaagaa gaagagaaag gtggaggcca gcggacgggc tgacgcattg gacgattttg
     6241 atctggatat gctgggaagt gacgccctcg atgattttga ccttgacatg cttggttcgg
     6301 atgcccttga tgactttgac ctcgacatgc tcggcagtga cgcccttgat gatttcgacc
     6361 tggacatgct gattaacggt accgctagcg gcagtggaga gggcagagga agtctgctaa
     6421 catgcggtga cgtcgaggag aatcctggcc cagtgagcaa gcttatggct tcaaacttta
     6481 ctcagttcgt gctcgtggac aatggtggga caggggatgt gacagtggct ccttctaatt
     6541 tcgctaatgg ggtggcagag tggatcagct ccaactcacg gagccaggcc tacaaggtga
     6601 catgcagcgt caggcagtct agtgcccaga agagaaagta taccatcaag gtggaggtcc
     6661 ccaaagtggc tacccagaca gtgggcggag tcgaactgcc tgtcgccgct tggaggtcct
     6721 acctgaacat ggagctcact atcccaattt tcgctaccaa ttctgactgt gaactcatcg
     6781 tgaaggcaat gcaggggctc ctcaaagacg gtaatcctat cccttccgcc atcgccgcta
     6841 actcaggtat ctacagcgct ggaggaggtg gaagcggagg aggaggaagc ggaggaggag
     6901 gtagcggacc taagaaaaag aggaaggtgg cggccgctgg atccccttca gggcagatca
     6961 gcaaccaggc cctggctctg gcccctagct ccgctccagt gctggcccag actatggtgc
     7021 cctctagtgc tatggtgcct ctggcccagc cacctgctcc agcccctgtg ctgaccccag
     7081 gaccacccca gtcactgagc gctccagtgc ccaagtctac acaggccggc gaggggactc
     7141 tgagtgaagc tctgctgcac ctgcagttcg acgctgatga ggacctggga gctctgctgg
     7201 ggaacagcac cgatcccgga gtgttcacag atctggcctc cgtggacaac tctgagtttc
     7261 agcagctgct gaatcagggc gtgtccatgt ctcatagtac agccgaacca atgctgatgg
     7321 agtaccccga agccattacc cggctggtga ccggcagcca gcggcccccc gaccccgctc
     7381 caactcccct gggaaccagc ggcctgccta atgggctgtc cggagatgaa gacttctcaa
     7441 gcatcgctga tatggacttt agtgccctgc tgtcacagat ttcctctagt gggcagggag
     7501 gaggtggaag cggcttcagc gtggacacca gtgccctgct ggacctgttc agcccctcgg
     7561 tgaccgtgcc cgacatgagc ctgcctgacc ttgacagcag cctggccagt atccaagagc
     7621 tcctgtctcc ccaggagccc cccaggcctc ccgaggcaga gaacagcagc ccggattcag
     7681 ggaagcagct ggtgcactac acagcgcagc cgctgttcct gctggacccc ggctccgtgg
     7741 acaccgggag caacgacctg ccggtgctgt ttgagctggg agagggctcc tacttctccg
     7801 aaggggacgg cttcgccgag gaccccacca tctccctgct gacaggctcg gagcctccca
     7861 aagccaagga ccccactgtc tcctaagaat tcctagagct cgctgatcag cctcgactgt
     7921 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     7981 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     8041 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     8101 agagaatagc aggcatgctg gggagcggcc gcaggaaccc ctagtgatgg agttggccac
     8161 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     8221 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct
     8281 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
     8341 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     8401 gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt
     8461 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8521 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     8581 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     8641 aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt
     8701 gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa
     8761 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
     8821 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     8881 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     8941 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     9001 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     9061 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     9121 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     9181 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     9241 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     9301 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     9361 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     9421 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     9481 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     9541 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     9601 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     9661 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     9721 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     9781 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     9841 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     9901 cgtggaagcc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     9961 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
    10021 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
    10081 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
    10141 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    10201 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    10261 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    10321 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
    10381 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    10441 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    10501 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    10561 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    10621 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    10681 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    10741 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    10801 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    10861 gctcacatg
//