| 质粒名称: | pJRH-1179 U6-reci Gag-Cas9 v2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 201914 |
| 存储实验室: | Jennifer Doudna |
|
载体骨架基本信息 |
|
| 载体名称: | pCAGGS |
| 载体出品公司: | Clontech |
| 空载体大小: | 11206bp |
| 完整质粒大小: | 11206bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Gag-Cas9 v2 |
| 启动子: | CAG |
| 克隆方法: | Gibson Cloning |
| 5’测序引物 | CTCTAGAGCCTCTGCTAACC |
| 3’测序引物 | GCCAGAAGTCAGATGCTCAAGG |
|
文献引用方法 |
|
| 材料和方法部分: | pJRH-1179 U6-reci Gag-Cas9 v2 was a gift from Jennifer Doudna (Addgene plasmid # 201914 ; http://n2t.net/addgene:201914 ; RRID:Addgene_201914) |
| 参考文献部分: | In vivo human T cell engineering with enveloped delivery vehicles. Hamilton JR, Chen E, Perez BS, Sandoval Espinoza CR, Kang MH, Trinidad M, Ngo W, Doudna JA. Nat Biotechnol. 2024 Jan 11. doi: 10.1038/s41587-023-02085-z. 10.1038/s41587-023-02085-z PubMed 38212493 |
买家导航
pJRH-1179 U6-reci Gag-Cas9 v2载体质粒基本信息
pJRH-1179 U6-reci Gag-Cas9 v2质粒图谱载体图谱和pJRH-1179 U6-reci Gag-Cas9 v2载体序列质粒序列多克隆位点信息
pJRH-1179 U6-reci Gag-Cas9 v2质粒载体简介
pJRH-1179 U6-reci Gag-Cas9 v2质粒序列载体序列
LOCUS pJRH-1179 U6-reci Gag-Cas9 v2 11206 bp ds-DNA circular SYN 04-AUG-2023
DEFINITION Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide
(CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for
spacer insertion).
ACCESSION .
VERSION .
KEYWORDS pJRH-1179 U6-reci Gag-Cas9 v2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11206)
TITLE bioRxiv - Programmable enveloped delivery vehicles for human genome
engineering in vivo
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 11206)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 4, 2023 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..11206
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 82..322
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 82..102
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 253..272
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
enhancer 489..868
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
intron 1150..2166
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 2090..2112
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 2174..2193
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
regulatory 2221..2230
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2227..3726
/codon_start=1
/gene="gag"
/product="gag protein from human immunodeficiency virus 1"
/label=HIV-1 gag
/translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
CDS 3727..3753
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
CDS 3766..3792
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
CDS 3805..3831
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
CDS 4003..8106
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 8110..8175
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 8194..8214
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 8221..8241
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind complement(8254..8273)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 8319..8374
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(8373..8392)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(8735..8751)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8735..8751)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8748..8770)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8759..8775
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8783..8813)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8828..8849
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8908..9103
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 8954..9089
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9016..9035
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 9109..9243
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9146..9165)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9200..9219
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(9311..9328)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9482..10070)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9562..9581)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10241..11101)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10864..10883
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11102..11206)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 cacatttccc cgaaaagtgc cacctggtcg acgggtttat tacagggaca gcagagatcc
61 agtttggtta attaaggtac cgagggccta tttcccatga ttccttcata tttgcatata
121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
301 ttatatatct tgtggaaagg acgaaacacc ggagacggag gacgacgaac gtctctgttt
361 aagagctatg ctggaaacag catagcaagt ttaaataagg ctagtccgtt atcaacttga
421 aaaagtggca ccgagtcggt gctttttttg aattcgctag ctaggtcttg aaaggagtgg
481 gaattgtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
541 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
601 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
661 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
721 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
781 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
841 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
901 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
961 tgcagcgatg ggggcggggg gggggggggg gggcgcgcgc srggsggggs ggggsggggs
1021 grggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc
1081 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
1141 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
1201 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
1261 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
1321 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
1381 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
1441 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
1501 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
1561 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
1621 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
1681 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
1741 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg
1801 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
1861 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc
1921 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg
1981 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg
2041 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
2101 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
2161 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc
2221 gccaccatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg atgggaaaaa
2281 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
2341 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
2401 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
2461 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
2521 gaagctttag acaagataga ggaagagcaa aacaaaagta agaaaaaagc acagcaagca
2581 gcagctgaca caggacacag caatcaggtc agccaaaatt accctatagt gcagaacatc
2641 caggggcaaa tggtacatca ggccatatca cctagaactt taaatgcatg ggtaaaagta
2701 gtagaagaga aggctttcag cccagaagtg atacccatgt tttcagcatt atcagaagga
2761 gccaccccac aagatttaaa caccatgcta aacacagtgg ggggacatca agcagccatg
2821 caaatgttaa aagagaccat caatgaggaa gctgcagaat gggatagagt gcatccagtg
2881 catgcagggc ctattgcacc aggccagatg agagaaccaa ggggaagtga catagcagga
2941 actactagta cccttcagga acaaatagga tggatgacac ataatccacc tatcccagta
3001 ggagaaatct ataaaagatg gataatcctg ggattaaata aaatagtaag aatgtatagc
3061 cctaccagca ttctggacat aagacaagga ccaaaggaac cctttagaga ctatgtagac
3121 cgattctata aaactctaag agccgagcaa gcttcacaag aggtaaaaaa ttggatgaca
3181 gaaaccttgt tggtccaaaa tgcgaaccca gattgtaaga ctattttaaa agcattggga
3241 ccaggagcga cactagaaga aatgatgaca gcatgtcagg gagtgggggg acccggccat
3301 aaagcaagag ttttggctga agcaatgagc caagtaacaa atccagctac cataatgata
3361 cagaaaggca attttaggaa ccaaagaaag actgttaagt gtttcaattg tggcaaagaa
3421 gggcacatag ccaaaaattg cagggcccct aggaaaaagg gctgttggaa atgtggaaag
3481 gaaggacacc aaatgaaaga ttgtactgag agacaggcta attttttagg gaagatctgg
3541 ccttcccaca agggaaggcc agggaatttt cttcagagca gaccagagcc aacagcccca
3601 ccagaagaga gcttcaggtt tggggaagag acaacaactc cctctcagaa gcaggagccg
3661 atagacaagg aactgtatcc tttagcttcc ctcagatcac tctttggcag cgacccctcg
3721 tcacaactcc cccctctgga gcgccttact cttggctccc tgcagttgcc cccactggag
3781 cggctgaccc tggggtcatt gcagttgcct ccactcgaac gactgacgtt gagccagaac
3841 tatccgattg tgcagaccgg tatgaagcga gcactgccca acaacaccag ctcctctccc
3901 cagccaaaga agaaaccagg agggtcaggg ggaaagcgag cactgcccaa caacaccagc
3961 tcctctcccc agccaaagaa gaaaccagga gggtcagggg gaatggacaa gaagtacagc
4021 atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
4081 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
4141 ctgatcggcg ccctgctgtt cgacagcgga gaaacagccg aggccacccg gctgaagaga
4201 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
4261 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
4321 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
4381 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
4441 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
4501 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
4561 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
4621 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
4681 gcccagctgc ccggcgagaa gaagaatggc ctgttcggca acctgattgc cctgagcctg
4741 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
4801 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
4861 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
4921 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
4981 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
5041 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat cgatggcgga
5101 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
5161 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
5221 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
5281 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
5341 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
5401 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgcc
5461 agcgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
5521 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtacaacga gctgaccaaa
5581 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaaa
5641 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
5701 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
5761 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
5821 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
5881 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
5941 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
6001 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
6061 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
6121 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
6181 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
6241 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
6301 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
6361 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
6421 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
6481 gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt
6541 ctgaaggacg actccatcga taacaaagtg ctgactcgga gcgacaagaa ccggggcaag
6601 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcgccagctg
6661 ctgaatgcca agctgattac ccagaggaag ttcgacaatc tgaccaaggc cgagagaggc
6721 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
6781 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaac
6841 gacaaactga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
6901 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
6961 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
7021 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
7081 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
7141 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac
7201 ggcgaaacag gcgagatcgt gtgggataag ggccgggact ttgccaccgt gcggaaagtg
7261 ctgtctatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
7321 aaagagtcta tcctgcccaa gaggaacagc gacaagctga tcgccagaaa gaaggactgg
7381 gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg
7441 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
7501 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
7561 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
7621 gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg
7681 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
7741 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa acactacctg
7801 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
7861 ctggacaagg tgctgagcgc ctacaacaag cacagagaca agcctatcag agagcaggcc
7921 gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac
7981 tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc
8041 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
8101 ggcgacgccg actataagga ccacgacgga gactacaagg atcatgatat tgattacaaa
8161 gacgatgacg ataagagcct gggcagcggc tcccccaaga aaaaacgcaa ggtggaagat
8221 cctaagaaaa agcggaaagt gtaagctagc agatcttttt ccctctgcca aaaattatgg
8281 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
8341 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
8401 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
8461 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
8521 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
8581 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
8641 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc
8701 cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
8761 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
8821 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
8881 cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc
8941 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
9001 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
9061 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt
9121 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
9181 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
9241 atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
9301 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
9361 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
9421 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
9481 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
9541 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
9601 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
9661 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
9721 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
9781 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
9841 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
9901 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
9961 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
10021 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
10081 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
10141 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
10201 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
10261 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
10321 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
10381 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
10441 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
10501 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
10561 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
10621 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
10681 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
10741 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
10801 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
10861 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
10921 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
10981 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
11041 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
11101 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
11161 acatatttga atgtatttag aaaaataaac aaataggggt tccgcg
//
