pTCK303-bk1-5Myc-bk2

LOCUS       pTCK303-bk1-5Myc-bk2              15585 bp DNA     circular SYN 25-MAR-2026
DEFINITION  pTCK303.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..15585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(23..368)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     745..761
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        777..2769
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     misc_feature    2686..2703
                     /label=303F
     misc_feature    2767..2787
                     /label=同源臂
     CDS             2818..2847
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2857..2886
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2896..2925
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2935..2964
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2998..3027
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    3034..3054
                     /label=同源臂
     misc_feature    3067..3087
                     /label=同源臂
     CDS             3088..3097
                     /codon_start=1
                     /label=Feature 22
                     /translation="EFP"
     terminator      3098..3350
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3147..3166
                     /label=NOSR
     primer_bind     complement(3367..3383)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3391..3407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3415..3445)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3460..3481
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3672..4348
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4415..5440
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5481..5655
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5733..5757
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6182..6976
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      8262..8850
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    9036..9176
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(11282..11911)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13212..13236
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      13258..13510
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(13545..13562)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(13569..15386,15577..15585,1..6))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          15387..15576
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 taccatggtc aagagtcccc cgtgttctct ccaaatgaaa tgaacttcct tatatagagg
       61 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
      121 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
      181 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttcaacg atggcctttc
      241 ctttatcgca atgatggcat ttgtaggagc caccttcctt ttccactatc ttcacaataa
      301 agtgacagat agctgggcaa tggaatccga ggaggtttcc ggatattacc ctttgttgaa
      361 aagtctcaat tgccctttgg tcttctgaga ctgtatcttt gatatttttg gagtagacaa
      421 gtgtgtcgtg ctccaccatg ttgacgaaga ttttcttctt gtcattgagt cgtaagagac
      481 tctgtatgaa ctgttcgcca gtctttacgg cgagttctgt taggtcctct atttgaatct
      541 ttgactccat gaagctaaac tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc
      601 tctagcattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
      661 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
      721 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc
      781 agtgcagcgt gacccggtcg tgcccctctc tagagataat gagcattgca tgtctaagtt
      841 ataaaaaatt accacatatt ttttttgtca cacttgtttg aagtgcagtt tatctatctt
      901 tatacatata tttaaacttt actctacgaa taatataatc tatagtacta caataatatc
      961 agtgttttag agaatcatat aaatgaacag ttagacatgg tctaaaggac aattgagtat
     1021 tttgacaaca ggactctaca gttttatctt tttagtgtgc atgtgttctc cttttttttt
     1081 gcaaatagct tcacctatat aatacttcat ccattttatt agtacatcca tttagggttt
     1141 agggttaatg gtttttatag actaattttt ttagtacatc tattttattc tattttagcc
     1201 tctaaattaa gaaaactaaa actctatttt agttttttta tttaataatt tagatataaa
     1261 atagaataaa ataaagtgac taaaaattaa acaaataccc tttaagaaat taaaaaaact
     1321 aaggaaacat ttttcttgtt tcgagtagat aatgccagcc tgttaaacgc cgtcgacgag
     1381 tctaacggac accaaccagc gaaccagcag cgtcgcgtcg ggccaagcga agcagacggc
     1441 acggcatctc tgtcgctgcc tctggacccc tctcgagagt tccgctccac cgttggactt
     1501 gctccgctgt cggcatccag aaattgcgtg gcggagcggc agacgtgagc cggcacggca
     1561 ggcggcctcc tcctcctctc acggcaccgg cagctacggg ggattccttt cccaccgctc
     1621 cttcgctttc ccttcctcgc ccgccgtaat aaatagacac cccctccaca ccctctttcc
     1681 ccaacctcgt gttgttcgga gcgcacacac acacaaccag atctccccca aatccacccg
     1741 tcggcacctc cgcttcaagg tacgccgctc gtcctccccc cccccccctc tctaccttct
     1801 ctagatcggc gttccggtcc atggttaggg cccggtagtt ctacttctgt tcatgtttgt
     1861 gttagatccg tgtttgtgtt agatccgtgc tgctagcgtt cgtacacgga tgcgacctgt
     1921 acgtcagaca cgttctgatt gctaacttgc cagtgtttct ctttggggaa tcctgggatg
     1981 gctctagccg ttccgcagac gggatcgatt tcatgatttt ttttgtttcg ttgcataggg
     2041 tttggtttgc ccttttcctt tatttcaata tatgccgtgc acttgtttgt cgggtcatct
     2101 tttcatgctt ttttttgtct tggttgtgat gatgtggtct ggttgggcgg tcgttctaga
     2161 tcggagtaga attctgtttc aaactacctg gtggatttat taattttgga tctgtatgtg
     2221 tgtgccatac atattcatag ttacgaattg aagatgatgg atggaaatat cgatctagga
     2281 taggtataca tgttgatgcg ggttttactg atgcatatac agagatgctt tttgttcgct
     2341 tggttgtgat gatgtggtgt ggttgggcgg tcgttcattc gttctagatc ggagtagaat
     2401 actgtttcaa actacctggt gtatttatta attttggaac tgtatgtgtg tgtcatacat
     2461 cttcatagtt acgagtttaa gatggatgga aatatcgatc taggataggt atacatgttg
     2521 atgtgggttt tactgatgca tatacatgat ggcatatgca gcatctattc atatgctcta
     2581 accttgagta cctatctatt ataataaaca agtatgtttt ataattattt tgatcttgat
     2641 atacttggat gatggcatat gcagcagcta tatgtggatt tttttagccc tgccttcata
     2701 cgctatttat ttgcttggta ctgtttcttt tgtcgatgct caccctgttg tttggtgtta
     2761 cttctgcagg tcgactctcg aggatccggt acccggtatc gatttaaaag gcctatggag
     2821 caaaagctca tttctgaaga ggacttgaat gaaatggagc aaaagctcat ttctgaagag
     2881 gacttgaatg aaatggagca aaagctcatt tctgaagagg acttgaatga aatggagcaa
     2941 aagctcattt ctgaagagga cttgaatgaa atggagagct tgggcgacct caccatggag
     3001 caaaagctca tttctgaaga ggacttgagg cctcaggtcg actctcgagg atcaactagt
     3061 gagctcagta cccggtatcg atttaaagaa tttccccgat cgttcaaaca tttggcaata
     3121 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     3181 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     3241 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     3301 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattcgt
     3361 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     3421 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     3481 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     3541 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt ggctagagca gcttgccaac
     3601 atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac
     3661 caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat
     3721 tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa
     3781 tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc
     3841 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
     3901 tcaaagcaag tggattgatg tgaacatggt ggagcacgac actctcgtct actccaagaa
     3961 tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
     4021 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt
     4081 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
     4141 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
     4201 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga
     4261 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
     4321 ttcatttcat ttggagagga cacgctgaaa tcaccagtct ctctctacaa atctatctct
     4381 ctcgagcttt cgcagatccg gggggcaatg agatatgaaa aagcctgaac tcaccgcgac
     4441 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     4501 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     4561 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     4621 ggccgcgctc ccgattccgg aagtgcttga cattggggag tttagcgaga gcctgaccta
     4681 ttgcatctcc cgccgttcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     4741 cgctgttcta caaccggtcg cggaggctat ggatgcgatc gctgcggccg atcttagcca
     4801 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     4861 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     4921 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     4981 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     5041 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     5101 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     5161 cgagcggagg catccggagc ttgcaggatc gccacgactc cgggcgtata tgctccgcat
     5221 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     5281 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     5341 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     5401 aaaccgacgc cccagcactc gtccgagggc aaagaaatag agtagatgcc gaccgggatc
     5461 tgtcgatcga caagctcgag tttctccata ataatgtgtg agtagttccc agataaggga
     5521 attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
     5581 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     5641 actaaaatcc agatcccccg aattaattcg gcgttaattc agtacattaa aaacgtccgc
     5701 aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca
     5761 gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc
     5821 ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg
     5881 ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct
     5941 cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaccgcg
     6001 gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac aactttgaaa
     6061 aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg gagttcgtct
     6121 tgttataatt agcttcttgg ggtatcttta aatactgtag aaaagaggaa ggaaataata
     6181 aatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat accgctgcgt
     6241 aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa
     6301 cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg tggaacggga
     6361 aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga
     6421 acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc tttgctcgga
     6481 agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag
     6541 gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag acagccgctt
     6601 agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg aaaactggga
     6661 agaagacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga cggaaaagcc
     6721 cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct ttgtgaaaga
     6781 tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca agtggtatga
     6841 cattgccttc tgcgtccggt cgatcaggga ggatatcggg gaagaacagt atgtcgagct
     6901 attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt atattttact
     6961 ggatgaattg ttttagtacc tagggaaggt gcgaataagc ggggaaattc ttctcggctg
     7021 actcagtcat ttcatttctt catgtttgag ccgatttttt ctcccgtaaa tgccttgaat
     7081 cagcctattt agaccgtttc ttcgccattt aaggcgttat ccccagtttt tagtgagatc
     7141 tctcccactg acgtatcatt tggtccgccc gaaacaggtt ggccagcgtg aataacatcg
     7201 ccagttggtt atcgtttttc agcaacccct tgtatctggc tttcacgaag ccgaactgtc
     7261 gcttgatgat gcgaaatggg tgctccaccc tggcccggat gctggctttc atgtattcga
     7321 tgttgatggc cgttttgttc ttgcgtggat gctgtttcaa ggttcttacc ttgccggggc
     7381 gctcggcgat cagccagtcc acatccacct cggccagctc ctcgcgctgt ggcgcccctt
     7441 ggtagccggc atcggctgag acaaattgct cctctccatg cagcagatta cccagctgat
     7501 tgaggtcatg ctcgttggcc gcggtggtga ccaggctgtg ggtcaggcca ctcttggcat
     7561 cgacaccaat gtgggccttc atgccaaagt gccactgatt gcctttcttg gtctgatgca
     7621 tctccggatc gcgttgctgc tctttgttct tggtcgagct gggtgcctca atgatggtgg
     7681 catcgaccaa ggtgccttga gtcatcatga cgcctgcttc ggccagccag cgattgatgg
     7741 tcttgaacaa ttggcgggcc agttgatgct gctccagcag gtggcggaaa ttcatgatgg
     7801 tggtgcggtc cggcaaggcg ctatccaggg ataaccgggc aaacagacgc atggaggcga
     7861 tttcgtacag agcatcttcc atcgcgccat cgctcaggtt gtaccaatgc tgcatgcagt
     7921 gaatgcgtag catggtttcc agcggataag gtcgccggcc attaccagcc ttggggtaaa
     7981 acggctcgat gacttccacc atgttttgcc atggcagaat ctgctccatg cgggacaaga
     8041 aaatctcttt tctggtctga cggcgcttac tgctgaattc actgtcggcg aaggtaagtt
     8101 gatgactcat gatgaaccct gttctatggc tccagatgac aaacatgatc tcatatcagg
     8161 gacttgttcg caccttccct agaatgcatg accaaaatcc cttaacgtga gttttcgttc
     8221 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     8281 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     8341 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     8401 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     8461 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     8521 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     8581 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     8641 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     8701 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     8761 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8821 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8881 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     8941 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     9001 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     9061 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     9121 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     9181 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     9241 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     9301 cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg
     9361 gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg
     9421 gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc
     9481 gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt
     9541 ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt
     9601 atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac
     9661 gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaag
     9721 agaccttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat
     9781 taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg
     9841 ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc
     9901 ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc
     9961 gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga
    10021 aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg
    10081 ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc
    10141 ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat
    10201 accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg
    10261 tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg
    10321 cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc
    10381 ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg
    10441 taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga
    10501 agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc
    10561 acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa
    10621 tctggttgct cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt
    10681 tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct
    10741 tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca
    10801 tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt
    10861 cctcgggctt gggggttcca gtgccattgc agggccggca ggcaacccag ccgcttacgc
    10921 ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg cctggttgtt
    10981 cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt tattcatttg
    11041 ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat ggtcttgcct
    11101 tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg aaagttgacc
    11161 cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct gctgcgtgcg
    11221 ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt acctcattaa
    11281 ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc
    11341 cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg tagctcacgc
    11401 gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc tcggcaacct
    11461 caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct tgtagccttc
    11521 catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg gcccatatgt
    11581 cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc gcggacacag
    11641 ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct
    11701 tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt tgatcttccc
    11761 gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac agaacatcgg
    11821 ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg cctgacccgc
    11881 ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt gtttatttac
    11941 tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa atacacatca
    12001 cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc aggccgccag
    12061 cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggttgaga cgtgcgcggg
    12121 cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa
    12181 cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt cctcttggcg ttcattctcg
    12241 gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg
    12301 gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc
    12361 acgccaagca gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg
    12421 gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc caaacttcac gcctcgggcc
    12481 ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc gaactcggca
    12541 atgccggcga acacggtcaa caccatgcgg ccggccggcg tggtggtgtc ggcccacggc
    12601 tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat gtccagtagg
    12661 tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg tcacaacgtc gccagggcgt
    12721 aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc tggtgccggt gatcttctcg
    12781 gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc gttggttggt caagtcctgg
    12841 tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg cggcgctctt gttcatggcg
    12901 taatgtctcc ggttctagtc gcaagtattc tactttatgc gactaaaaca cgcgacaaga
    12961 aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg
    13021 tcgttttcag aagacggctg cactgaacgt cagaagccga ctgcactata gcagcggagg
    13081 ggttggatca aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat
    13141 ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gattattcta ataaacgctc
    13201 ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt aattcccgat
    13261 ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt
    13321 tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa
    13381 ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata
    13441 tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt
    13501 ttgaacgatc ggggaaattc gagctggtca cctgtaattc acacgtggtg gtggtggtgg
    13561 tggctagctt gtttgcctcc ctgctgcggt ttttcaccga agttcatgcc agtccagcgt
    13621 ttttgcagca gaaaagccgc cgacttcggt ttgcggtcgc gagtgaagat ccctttcttg
    13681 ttaccgccaa cgcgcaatat gccttgcgag gtcgcaaaat cggcgaaatt ccatacctgt
    13741 tcaccgacga cggcgctgac gcgatcaaag acgcggtgat acatatccag ccatgcacac
    13801 tgatactctt cactccacat gtcggtgtac attgagtgca gcccggctaa cgtatccacg
    13861 ccgtattcgg tgatgataat cggctgatgc agtttctcct gccaggccag aagttctttt
    13921 tccagtacct tctctgccgt ttccaaatcg ccgctttgga cataccatcc gtaataacgg
    13981 ttcaggcaca gcacatcaaa gagatcgctg atggtatcgg tgtgagcgtc gcagaacatt
    14041 acattgacgc aggtgatcgg acgcgtcggg tcgagtttac gcgttgcttc cgccagtggc
    14101 gcgaaatatt cccgtgcacc ttgcggacgg gtatccggtt cgttggcaat actccacatc
    14161 accacgcttg ggtggttttt gtcacgcgct atcagctctt taatcgcctg taagtgcgct
    14221 tgctgagttt ccccgttgac tgcctcttcg ctgtacagtt ctttcggctt gttgcccgct
    14281 tcgaaaccaa tgcctaaaga gaggttaaag ccgacagcag cagtttcatc aatcaccacg
    14341 atgccatgtt catctgccca gtcgagcatc tcttcagcgt aagggtaatg cgaggtacgg
    14401 taggagttgg ccccaatcca gtccattaat gcgtggtcgt gcaccatcag cacgttatcg
    14461 aatcctttgc cacgcaagtc cgcatcttca tgacgaccaa agccagtaaa gtagaacggt
    14521 ttgtggttaa tcaggaactg ttcgcccttc actgccactg accggatgcc gacgcgaagc
    14581 gggtagatat cacactctgt ctggcttttg gctgtgacgc acagttcata gagataacct
    14641 tcacccggtt gccagaggtg cggattcacc acttgcaaag tcccgctagt gccttgtcca
    14701 gttgcaacca cctgttgatc cgcatcacgc agttcaacgc tgacatcacc attggccacc
    14761 acctgccagt caacagacgc gtggttacag tcttgcgcga catgcgtcac cacggtgata
    14821 tcgtccaccc aggtgttcgg cgtggtgtag agcattacgc tgcgatggat tccggcatag
    14881 ttaaagaaat catggaagta agactgcttt ttcttgccgt tttcgtcggt aatcaccatt
    14941 cccggcggga tagtctgcca gttcagttcg ttgttcacac aaacggtgat acgtacactt
    15001 ttcccggcaa taacatacgg cgtgacatcg gcttcaaatg gcgtatagcc gccctgatgc
    15061 tccatcactt cctgattatt gacccacact ttgccgtaat gagtgaccgc atcgaaacgc
    15121 agcacgatac gctggcctgc ccaacctttc ggtataaaga cttcgcgctg ataccagacg
    15181 ttgcccgcat aattacgaat atctgcatcg gcgaactgat cgttaaaact gcctggcaca
    15241 gcaattgccc ggctttcttg taacgcgctt tcccaccaac gctgatcaat tccacagttt
    15301 tcgcgatcca gactgaatgc ccacaggccg tcgagttttt tgatttcacg ggttggggtt
    15361 tctacaggac ggacgagtcg tcggttctgt aactatcatc atcatcatag acacacgaaa
    15421 taaagtaatc agattatcag ttaaagctat gtaatattta caccataacc aatcaattaa
    15481 aaaatagatc agtttaaaga aagatcaaag ctcaaaaaaa taaaaagaga aaagggtcct
    15541 aaccaagaaa atgaaggaga aaaactagaa atttaccctc agatc
//