| 质粒名称: | Tet-O-FUW-Myt1l |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 27152 |
| 存储实验室: | Marius Wernig |
|
载体骨架基本信息 |
|
| 载体名称: | TetO-FUW |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 8373 bp |
| 完整质粒大小: | 11950 bp |
| 载体类型: | 哺乳细胞表达，Lentiviral |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Myt1l |
| 物种来源: | M. musculus (老鼠) |
| 插入大小: | 3577 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoR1(未破坏) |
| 3' 克隆位点: | EcoR1(未破坏) |
| 5' 测序引物 | LNCX |
| 3' 测序引物 | WPRE-R |
|
文献引用方法 |
|
| 材料和方法部分: | Tet-O-FUW-Myt1l was a gift from Marius Wernig (爱迪基因 plasmid # 27152) |
| 参考文献部分: | Direct conversion of fibroblasts to functional neurons by defined factors. Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Sudhof TC, Wernig M. Nature. 2010 Feb 25. 463(7284):1035-41. 10.1038/nature08797 PubMed 20107439 |
买家导航
Tet-O-FUW-Myt1l载体质粒基本信息
Tet-O-FUW-Myt1l质粒图谱载体图谱和Tet-O-FUW-Myt1l载体序列质粒序列多克隆位点信息
Tet-O-FUW-Myt1l质粒载体简介
Tet-O-FUW-Myt1l质粒序列载体序列
LOCUS Tet-O-FUW-Myt1l 11956 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Tet-O-FUW-Myt1l
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11956)
AUTHORS Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Sudhof TC, Wernig M
TITLE Direct conversion of fibroblasts to functional neurons by defined
factors.
JOURNAL Nature. 2010 Feb 25. 463(7284):1035-41.
PUBMED 20107439
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|Tet-O-FUW-Myt1l|
FEATURES Location/Qualifiers
source 1..11956
/organism="synthetic DNA construct"
/mol_type="other DNA"
regulatory 9..18
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
misc_feature 3603..4191
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3656..3676)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4074..4085)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(4194..4210)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4195..4211)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 4716..4896
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(4922..4939)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4928..5152
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5198..5626
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5285..5304)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5495..5516
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5635..5655)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5640..5969
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5820..5955
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5882..5901
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 6017..6064
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 6083..6457
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 6587..6708
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6624..6643)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6678..6697
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(6757..6773)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6757..6773)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6770..6792)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6781..6797
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6805..6835)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6850..6871
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6988..7005)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7159..7747)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7239..7258)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7918..8778)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8541..8560
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8779..8883)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8958..8977)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 9149..9528
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 9530..9728
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 9683..9703
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 9746..9926
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 9973..10098
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 10591..10824
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 11351..11468
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 11522..11792
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
promoter 11825..11862
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 11859..11883
/label=LNCX
/note="Human CMV promoter, forward primer"
ORIGIN
1 aattgctagc caccatggac gtggactctg aggagaagcg ccatcgcaca cggtccaaag
61 gggttcgagt tcctgtggag ccagccatac aagagctgtt cagctgtccc actccaggct
121 gcgacggcag tggtcacgtc agtggcaaat atgcacgaca cagaagtgta tatggttgtc
181 ccttggctaa aaaaagaaaa acgcaagata aacagcccca agaacctgct cccaagcgaa
241 aaccatttgc agtaaaagca gatagttcct cagtagacga atgttatgag agtgatggta
301 ctgaagacat ggatgataag gaggaagatg atgatgagga gttctctgaa gacaatgatg
361 agcaagggga tgatgacgac gaagatgagg tggatcggga agacgaggag gagatcgagg
421 aggaagatga tgaagaagat gatgatgatg aagatggtga cgatgtagaa gaggaagaag
481 aggatgatga tgaagaggag gaagaagagg aagaggaaga agaaaatgaa gaccatcaaa
541 tgagttgtac tcgaataatg caggacacag acaaggatga taacaacaat gatgagtatg
601 ataactatga tgaactggta gctaagtcgc tattaaatct tggcaaaatt gctgaggatg
661 cagcataccg agccaggact gaatcagaga tgaacagcaa tacctccaat agtctggagg
721 acgatagtga caaaaacgaa aacctcggtc ggaaaagcga actgagtcta gacttagaca
781 gtgatgttgt tagagaaaca gtggactccc ttaagctgtt agcacaagga catggtgttg
841 tgctatcaga gaatatcagt gacagaagtt atgctgaggg gatgtcacag caggacagta
901 gaaatatgaa ctatgtcatg ctagggaagc ccatgaacaa tggactcatg gagaagatgg
961 tggaggagag tgatgaggaa gtgtgtctaa gtagtctaga gtgcctgagg aaccagtgct
1021 ttgacctggc caggaaactc agcgagacca acccacagga caggagtcag ccacccaaca
1081 tgagtgtgcg ccaacatgtc cggcaagagg acgacttccc tgggaggacg ccagacagga
1141 gctactcgga tatgatgaac cttatgcggc tggaggagca gctcagtccc aggtctagaa
1201 cgttctccag ctgtgccaag gaggatgggt gtcatgagag ggatgatgac accacctcag
1261 tgaactcaga caggtctgag gaagtgtttg acatgaccaa gggcaacctg actctgctag
1321 agaaagccat tgccttggag acagagagag ccaaggccat gcgggagaag atggccatgg
1381 atgctgggag aagggataac ctgagatcct atgaggacca gtctccaaga cagctggctg
1441 gggaagacag aaaatccaaa tccagtgaca gccatgtcaa aaagccatac tatggtaaag
1501 atccctcaag aacagaaaag agagagagca agtgtccaac ccccgggtgt gatggaaccg
1561 gccacgtaac tgggctttac ccgcatcacc gcagtctgtc tggatgcccg cacaaagata
1621 gggtccctcc agaaattctt gccatgcatg aaaatgttct caagtgtccc actccaggct
1681 gcacagggcg agggcatgtg aatagcaaca ggaactcgca cagaagcctc tctggatgcc
1741 ccattgctgc tgcagaaaaa ctggcaaagg cccaagagaa acaccagagc tgtgatgtgt
1801 ccaaatccaa ccaggcctca gaccgagtcc tcaggccaat gtgctttgtc aaacagcttg
1861 agattcctca gtatggctac agaaacaatg ttcccacaac cacaccacgc tccaacctgg
1921 ccaaggagct tgagaaatac tccaagactt cgtttgagta caacagttac gacaaccata
1981 cttatggcaa aagagccata gctcccaagg tgcaaaccag ggacatatcc cccaaaggat
2041 atgacgatgc caagcggtac tgcaagaatg ccagccccag cagcagcacc accagcagct
2101 atgcacctag cagcagcagc aacctcagct gtggtggtgg cagcagcgcc agtagcacgt
2161 gtagcaagag cagctttgac tacacacatg acatggaggc cgcacacatg gcagccacag
2221 ccattctcaa cctgtccaca cgttgtcgtg aaatgccaca gaacctgtcc accaagccac
2281 aggacctgtg tactgcccgg aacccagaca tggaggtgga tgagaatggc accctggacc
2341 tgagcatgaa caagcagagg cctcgagaca gctgctgccc agtcctgaca cccctggaac
2401 ccatgtctcc gcagcagcag gccgtgatga gcagccgatg cttccagctg agcgaggggg
2461 attgctggga cttgcctgta gactacacca aaatgaagcc tcggagggta gatgaggatg
2521 agcccaaaga gattacccca gaagacttgg acccattcca ggaggctctg gaagaaagac
2581 ggtatccagg ggaggtgacc atcccaagcc ccaaacccaa gtaccctcag tgcaaggaaa
2641 gcaaaaagga cttaataact ctgtctggct gccccctggc ggacaaaagc attcgaagta
2701 tgctggccac cagttcccaa gagctcaagt gccccacccc tggctgtgac ggttctggac
2761 acatcactgg caattacgct tctcatcgaa gcctttctgg gtgcccgaga gcaaagaaga
2821 gtggcatccg gatagcacag agcaaagagg acaaggaaga ccaggagcca atcaggtgtc
2881 cggtacctgg ctgtgacggt cagggacaca tcactgggaa gtatgcatcc caccgcagcg
2941 cctccgggtg tcccttggca gccaagaggc agaaagatgg gtaccttaat ggctcccagt
3001 tctcctggaa gtcggtcaag acggagggca tgtcctgccc tacccccggg tgtgatgggt
3061 caggacacgt cagtggcagc ttcctcacac accgcagctt gtcaggatgt ccaagagcca
3121 catcagcaat gaagaaagca aagctgtctg gagaacagat gttgactatc aagcagcgag
3181 ccagcaacgg tatagaaaat gatgaagaaa tcaagcagtt agatgaagag atcaaggagc
3241 ttaatgagtc caattcccag atggaggctg acatgatcaa actcagaact cagatcacca
3301 caatggagag caacctgaag acgattgagg aggagaacaa agtcattgaa cagcagaatg
3361 agtcgctctt gcacgagttg gccaacctga gccagtccct gatccacagc ctcgccaaca
3421 tccagctgcc tcacatggat ccaatcaatg aacaaaattt tgatgcttac gtgactactt
3481 tgacggaaat gtatacaaat caagatcgtt atcagagtcc agaaaataaa gccctactgg
3541 aaaatataaa gcaggctgtg agaggaattc aggtctgaca attcgatatc aagcttatcg
3601 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
3661 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
3721 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
3781 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
3841 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
3901 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
3961 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
4021 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
4081 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
4141 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
4201 gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
4261 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
4321 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
4381 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
4441 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
4501 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
4561 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
4621 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
4681 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
4741 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
4801 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
4861 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
4921 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
4981 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
5041 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
5101 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
5161 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
5221 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
5281 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
5341 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
5401 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
5461 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
5521 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
5581 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
5641 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
5701 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
5761 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
5821 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
5881 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
5941 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
6001 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
6061 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
6121 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
6181 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
6241 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
6301 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
6361 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
6421 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
6481 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
6541 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
6601 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
6661 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
6721 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
6781 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
6841 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
6901 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
6961 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
7021 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
7081 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
7141 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
7201 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
7261 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
7321 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
7381 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
7441 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
7501 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
7561 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
7621 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
7681 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
7741 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
7801 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
7861 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
7921 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
7981 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
8041 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
8101 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
8161 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
8221 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
8281 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
8341 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
8401 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
8461 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
8521 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
8581 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
8641 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
8701 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
8761 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
8821 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
8881 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
8941 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
9001 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
9061 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
9121 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
9181 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
9241 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
9301 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
9361 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
9421 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
9481 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
9541 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
9601 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
9661 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
9721 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
9781 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
9841 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
9901 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
9961 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
10021 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
10081 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
10141 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
10201 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
10261 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
10321 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
10381 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
10441 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
10501 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
10561 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
10621 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
10681 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
10741 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
10801 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
10861 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
10921 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
10981 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
11041 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
11101 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
11161 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
11221 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
11281 cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat
11341 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
11401 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
11461 aaaattttcg ggtttattac agggacagca gagatccagt ttggttactc gagtttacca
11521 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
11581 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
11641 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
11701 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
11761 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc ggtacccggg
11821 tcgagtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
11881 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
11941 agcctccgcg gccccg
//
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