| 质粒名称: | pLexm |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 99844 |
| 存储实验室: | Edith Yvonne Jones |
|
载体骨架基本信息 |
|
| 载体名称: | pCAG.I-SceI |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLexm was a gift from Edith Yvonne Jones (Addgene plasmid # 99844 ; http://n2t.net/addgene:99844 ; RRID:Addgene_99844) |
| 参考文献部分: | A time- and cost-efficient system for high-level protein production in mammalian cells. Aricescu AR, Lu W, Jones EY. Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1243-50. Epub 2006 Sep 19. 10.1107/S0907444906029799 PubMed 17001101 |
买家导航
pLexm载体质粒基本信息
pLexm质粒图谱载体图谱和pLexm载体序列质粒序列多克隆位点信息
pLexm质粒载体简介
pLexm质粒序列载体序列
LOCUS pLexm 4541 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION mammalian expression.
ACCESSION .
VERSION .
KEYWORDS pLexm
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4541)
AUTHORS Aricescu AR, Lu W, Jones EY
TITLE A time- and cost-efficient system for high-level protein production
in mammalian cells.
JOURNAL Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1243-50.
Epub 2006 Sep 19.
PUBMED 17001101
REFERENCE 2 (bases 1 to 4541)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4541
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 5..384
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 386..663
/label=chicken beta-actin promoter
intron 664..1680
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1604..1626
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1688..1707
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
primer_bind complement(1896..1915)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 1961..2016
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(2015..2034)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(2377..2393)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2377..2393)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(2390..2412)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 2401..2417
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2425..2455)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2470..2491
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(2620..2637)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2791..3379)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2871..2890)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3550..4410)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4173..4192
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4411..4515)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
481 gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg
541 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
601 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
661 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
721 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
781 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
841 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
901 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
961 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
1021 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
1081 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
1141 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
1201 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
1261 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc
1321 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
1381 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
1441 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
1501 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
1561 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
1621 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
1681 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgcggccg
1741 tctcaggccg aattcaacgt tggtaccgag ctcatcgatg cggccgcgca tgcctcgaga
1801 ctagtatcgc gataattcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
1861 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
1921 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
1981 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
2041 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
2101 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
2161 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
2221 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
2281 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
2341 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
2401 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
2461 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
2521 gtcgggaaac ctgtcgtgcc agcggatcga tccgctgcat taatgaatcg gccaacgcgc
2581 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
2641 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
2701 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
2761 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
2821 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
2881 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
2941 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
3001 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
3061 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
3121 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
3181 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
3241 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
3301 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
3361 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
3421 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
3481 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
3541 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
3601 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
3661 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
3721 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
3781 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
3841 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
3901 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
3961 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
4021 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
4081 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
4141 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
4201 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
4261 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
4321 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
4381 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
4441 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
4501 aataggggtt ccgcgcacat ttccccgaaa agtgccacct g
//
