| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-CHD7-C-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001734 |
| 产品用途: | 3' AAVLINK plasmid for CHD7 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: |
SCP1
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CHD7 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-CHD7-C-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-CHD7-C-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-CHD7-C-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-CHD7-C-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-CHD7-C-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-CHD7-C-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-CHD7-C-FLAG 7127 bp DNA circular SYN 21-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7127)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7127
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3831
/codon_start=1
/label=CHD7
/translation="VPKRMKRKSWRMTISRKSLPSPKQELSLEGRILMKKAMLP*ALLE
MKPEMDSTWRTEILQ*LSSFMKEHLPSRFGLRIE***TA*TTSVKQC*KANGQ*IGARC
LISKASSQVTHPPQWTAPCRRGALLSSPWSAKPALVGVRTSLRLLSCQRKMPSTSLSLA
SGGGGGEKSKLRPKELPRGEISWRWLPSFESLRWSQKMDKKKL*IYQRPQERQQALPQI
FHLFLQSLSCLMSQHQCLMPLRLKWNCSKQAFRAHPQGISLMAP*WMESLP*RGGGEGG
KMWRDLICFS*ATNGRH*VQRMLR*PKLLKKI*RPHQQETFLLPDSWTQTHGSLLSILK
MGLGWWGKMLLKIRI*LNG*SCTLLTLLICQVMYQRMQMCCFPHFRNRNRNDIDVETLI
NWI*TL*QEKKGCLLSINEMGRRWVELWRLQ*RIYPGGWKKILNLQLLQTGLI*LSSLV
LFLSRCLTAFSLGL*CGERERAEEEEGPKVRSPEQPRPPLLWPPRQGSTLCW*TACLLE
WT*RAFRISRISSRSSWQASWASLQDWQQLPPPEAMRRTLLLCCP*CCQEWRACPTCLA
WAGC*ITLCQLLLETPLLLLVKENRKTALQKERRKEMRMKTRTKTLRKAQMLFRLLTLR
MDLLVLLLPRLDCPQTR*PSTLSSCPQWPRASSTHPCFYLQDWGD*RCLGSQHWQDFRM
PWAPAKKRLLTRLREDPLKMERPLKAAMPRRAWIRLQSPPS*KTK*HRVKS*THLMGGM
K*KTMKMM"
misc_feature 2041..2046
/label=SDS
misc_feature 2044..2049
/label=SDS
misc_feature 2047..2052
/label=SDS
misc_feature 2371..2376
/label=SDS
misc_feature 2383..2388
/label=SDS
misc_feature 3519..3524
/label=SDS
CDS 3835..3900
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3916..4347
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4060..4065
/label=SDS
repeat_region 4390..4530
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4390..4519
/label=AAV2 ITR
rep_origin 4605..5060
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5342..5446
/gene="bla"
/label=AmpR promoter
CDS 5447..6307
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6478..7066
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggttc cgaagaggat
1561 gaagaggaaa agctggagga tgacgataag tcggaagagt cttcccagcc cgaagcagga
1621 gctgtctcta gagggaagaa ttttgatgaa gaaagcaatg cttccatgag cactgctaga
1681 gatgaaaccc gagatggatt ctacatggag gacggagatc cttcagtagc tcagctcctt
1741 catgaaagaa catttgcctt ctcgttttgg cctaaggata gagtaatgat aaaccgctta
1801 gacaacatct gtgaagcagt gttgaaaggc aaatggccag taaataggcg ccagatgttt
1861 gatttccaag gcctcatccc aggttacaca cccaccacag tggacagccc cttgcagaag
1921 aggagctttg ctgagctctc catggtcggc caagccagca ttagtgggag tgaggacatc
1981 actacgtctc ctcagttgtc aaaggaagat gccctcaacc tctctgtccc tcgccagcgg
2041 aggaggagga ggagaaaaat cgaaattgag gccgaaagag ctgccaagag gcgaaatctc
2101 atggagatgg ttgcccagct tcgagagtct caggtggtct cagaaaatgg acaagaaaaa
2161 gttgtagatt tatcaaaggc ctcaagagag gcaacaagct ctacctcaaa tttttcatct
2221 ctttcttcaa agtttatctt gcctaatgtc tcaacaccag tgtctgatgc ctttaagact
2281 caaatggaac tgctccaagc aggcctttcg cgcacaccca caaggcatct ccttaatggc
2341 tccctagtgg atggagagcc tcccatgaag aggaggcggg gaaggaggaa aaatgtggag
2401 ggacttgatc tgcttttcat gagccacaaa cggacgtcat tgagtgcaga ggatgctgag
2461 gtgaccaaag cttttgaaga agatatagag accccaccaa caagaaacat tccttctccc
2521 ggacagctgg acccagacac acggatccct gttatcaatc ttgaagatgg gactaggctg
2581 gtgggggaag atgctcctaa aaataaggat ttagttgaat ggctgaagct gcaccctact
2641 tacactgttg atatgccaag ttatgtacca aagaatgcag atgtgctgtt ttcctcattt
2701 cagaaaccga aacagaaacg acatagatgt cgaaacccta ataaattgga tataaacact
2761 ttgacaggag aagaaagggt gcctgttgtc aataaacgaa atgggaagaa gatgggtgga
2821 gctatggcgc ctccaatgaa ggatctaccc aggtggctgg aagaaaatcc tgaatttgca
2881 gttgctccag actggactga tatagttaag cagtctggtt ttgttcctga gtcgatgttt
2941 gaccgccttc tcactgggcc tgtagtgcgg ggagagggag cgagcagaag aggaagaagg
3001 cccaaaagtg agatcgccag agcagccgcg gccgccgctg ctgtggcctc cacgtcaggg
3061 atcaaccctt tgctggtgaa cagcctgttt gctggaatgg acctgacgag ccttcagaat
3121 ctccagaatc tccagtcgct ccagctggca ggcctcatgg gcttccctcc aggactggca
3181 acagctgcca ccgccggagg cgatgcgaag aaccctgctg ctgtgctgcc cctgatgctg
3241 ccaggaatgg cgggcctgcc caacgtgttt ggcttgggcg ggctgttgaa taaccctctg
3301 tcagctgcta ctggaaacac cactactgct tctagtcaag gagaaccgga agacagcact
3361 tcaaaaggag aggagaaagg aaatgagaat gaagacgaga acaaagactc tgagaaaagc
3421 acagatgctg tttcggctgc tgactctgcg aatggatctg ttggtgctgc tactgccccg
3481 gctggattgc cctcaaaccc gctagccttc aaccctttcc tcctgtccac aatggccccg
3541 ggcctcttct acccatccat gtttctacct ccaggactgg ggggattgac gctgcctggg
3601 ttcccagcat tggcaggact tcagaatgcc gtgggctcca gcgaagaaaa ggctgctgac
3661 aaggctgagg gaggaccctt taaagatgga gagacccttg aaggcagcga tgccgaggag
3721 agcctggata agactgcaga gtcctccctc ttagaagacg aaatagcaca gggtgaagag
3781 ctagactcac ttgatggggg ggatgaaata gaaaacaatg aaaatgatga aaaggactac
3841 aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag
3901 taagaattct gatgactcga gataatcaac ctctggatta caaaatttgt gaaagattga
3961 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
4021 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
4081 tagttcttgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
4141 ggctgttggg cactgacaat tccgtggtgt ttatttgtga aatttgtgat gctattgctt
4201 tatttgtaac catctagctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
4261 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
4321 cagggggaga tgtgggaggt tttttaaaga tctggagacc ggtgtggcac gtgcggaccg
4381 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
4441 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
4501 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
4561 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
4621 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
4681 cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
4741 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
4801 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
4861 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
4921 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
4981 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
5041 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
5101 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
5161 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
5221 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
5281 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
5341 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
5401 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
5461 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
5521 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
5581 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
5641 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
5701 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
5761 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
5821 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
5881 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
5941 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
6001 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
6061 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
6121 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
6181 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
6241 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
6301 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
6361 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
6421 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
6481 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
6541 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
6601 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
6661 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
6721 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
6781 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
6841 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
6901 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
6961 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
7021 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
7081 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
