| 质粒名称: | pADH119 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 90985 |
| 存储实验室: | Aaron Hernday |
|
载体骨架基本信息 |
|
| 载体名称: | pUC19 |
| 载体出品公司: | Clontech |
| 空载体大小: | 2242bp |
| 完整质粒大小: | 3094 |
| 载体类型: | CRISPR |
| 筛选标记: | Nourseothricin (NAT) |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | gRNA conserved, C. albicans LEU2 2 of 2 |
|
文献引用方法 |
|
| 材料和方法部分: | pADH119 was a gift from Aaron Hernday (Addgene plasmid # 90985 ; http://n2t.net/addgene:90985 ; RRID:Addgene_90985) |
| 参考文献部分: | An Efficient, Rapid, and Recyclable System for CRISPR-Mediated Genome Editing in Candida albicans. Nguyen N, Quail MMF, Hernday AD. mSphere. 2017 Apr 26;2(2):e00149-17. doi: 10.1128/mSphereDirect.00149-17. eCollection 2017 Mar-Apr. 10.1128/mSphereDirect.00149-17 PubMed 28497115 |
买家导航
pADH119载体质粒基本信息
pADH119质粒图谱载体图谱和pADH119载体序列质粒序列多克隆位点信息
pADH119质粒载体简介
pADH119质粒序列载体序列
LOCUS pADH119 3094 bp DNA circular SYN 14-APR-2025
DEFINITION NAT-marked ""empty"" gRNA construct for use in gRNA expression
cassette stitching PCR. Use with pADH110 to generate target-specific
part 2 of 2 for C.alb LEUpOUT CRISPR system..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3094)
AUTHORS Nguyen N, Quail MMF, Hernday AD
TITLE An Efficient, Rapid, and Recyclable System for CRISPR-Mediated
Genome Editing in Candida albicans.
JOURNAL mSphere. 2017 Apr 26;2(2):e00149-17. doi:
10.1128/mSphereDirect.00149-17. eCollection 2017 Mar-Apr.
PUBMED 28497115
REFERENCE 2 (bases 1 to 3094)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1128/mSphereDirect.00149-17"; journalName: "mSphere"; date:
"2017-04-26- 26"; volume: "2"; issue: "2"; pages: "e00149-17"
COMMENT Sequence Label: pADH119
FEATURES Location/Qualifiers
source 1..3094
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter complement(1..105)
/gene="bla"
/label=AmpR promoter
primer_bind 173..191
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
misc_RNA 225..300
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter complement(join(1121..1127,1128..1145,1146..1151))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1166..1187
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(1304..1321)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1475..2063)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1555..1574)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(2234..3025,3026..3094))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2857..2876
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
ORIGIN
1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
121 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
181 tatcacgagg ccctttgacg gcacggccac gcgtttaaac cgccgtttta gagctagaaa
241 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
301 tttttttctc gagttttttt atcgagttaa aagctgaagt ggtcaaagga accaatttaa
361 ttattgttcg tgaattagtt ggtggtatat attttggtga acgtcaagaa caagaagaaa
421 gtgaagacaa aaaaacagct tgggatacag aaaagtatac tgttgacgaa gttactcgta
481 tcactcgtat ggcagcattt atggctttgc agcacaatcc accattgcca atatggtctt
541 tggacaaagc taatgttttg gcatcgtcga gattgtggag aaagaccgtt gataaagtga
601 tttctgaaga atttccagct ttgtctgtgc agcatcaatt gattgattcg gctgcaatga
661 ttttgatcca aaacccaacc aaattgaacg gtatcatcat tacatctaac atgtttggtg
721 atatcatctc agatgaggca tccgttatcc caggatcttt gggtttattg ccatctgctt
781 cattggcttc attgccagat accaacactg cttttggtct ttatgagcct tgccatgggt
841 ctgctccaga tttaccagcc aacaaagtga atccaattgc tacaatcttg tctgccgctt
901 ccatgttgag attatccttg gattgtgtta aggaagctga agcattggaa gaagctgtca
961 aacaggtttt ggatagcggt atcagaacag cagatttaag aggtactagc tctactacag
1021 aagtcggtga cgcaatagcc gaaacagtca ctaaaatttt aaaacaaaat acataacggt
1081 gtttaaaccc cagcgcctgg cgggcgctca caattccaca caacatacga gccggaagca
1141 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
1201 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
1261 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
1321 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
1381 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
1441 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
1501 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
1561 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
1621 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
1681 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
1741 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
1801 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
1861 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
1921 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
1981 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
2041 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
2101 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
2161 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
2221 cttggtctga cagttaccaa tgcttgatca gtgaggcacc tatctcagcg atctgtctat
2281 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
2341 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
2401 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
2461 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
2521 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
2581 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
2641 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
2701 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
2761 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
2821 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
2881 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
2941 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
3001 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
3061 gaataagggc gacacggaaa tgttgaatac tcat
//
