| 质粒名称: | pcDNA3/HA-RBP2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 14799 |
| 存储实验室: | William Kaelin |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3 |
| 载体出品公司: | -- |
| 空载体大小: | 5446 bp |
| 完整质粒大小: | 11734 bp |
| 载体修饰: | |
| 载体类型: | 哺乳动物表达 |
| 筛选标记: | 新霉素（G418筛选） |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Retinoblastoma Binding Protein 2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 4900 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | HA（主干上的N终端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | pCAAT |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3/HA-RBP2 was a gift from William Kaelin (Addgene plasmid # 14799 ; http://n2t.net/addgene:14799 ; RRID:Addgene_14799) |
| 参考文献部分: | The retinoblastoma binding protein RBP2 is an H3K4 demethylase. Klose RJ, Yan Q, Tothova Z, Yamane K, Erdjument-Bromage H, Tempst P, Gilliland DG, Zhang Y, Kaelin WG. Cell. 2007 Mar 9. 128(5):889-900. 10.1016/j.cell.2007.02.013 PubMed 17320163 |
买家导航
pcDNA3/HA-RBP2载体质粒基本信息
pcDNA3/HA-RBP2质粒图谱载体图谱和pcDNA3/HA-RBP2载体序列质粒序列多克隆位点信息
pcDNA3/HA-RBP2质粒载体简介
pcDNA3/HA-RBP2质粒序列载体序列
LOCUS pcDNA3/HA-RBP2 11734 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pcDNA3/HA-RBP2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11734)
AUTHORS Klose RJ, Yan Q, Tothova Z, Yamane K, Erdjument-Bromage H, Tempst P,
Gilliland DG, Zhang Y, Kaelin WG
TITLE The retinoblastoma binding protein RBP2 is an H3K4 demethylase.
JOURNAL Cell. 2007 Mar 9. 128(5):889-900.
PUBMED 17320163
REFERENCE 2 (bases 1 to 11734)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..11734
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 907..933
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 1273..1284
/codon_start=1
/product="WELQut protease recognition and cleavage site"
/label=WELQut site
/translation="WELQ"
promoter complement(7286..7304)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(7287..7304)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(7324..7341)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 7330..7554
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 7600..8028
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7687..7706)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7897..7918
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(8037..8057)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 8042..8372
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 8223..8358
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8285..8304
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 8439..9233
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(8493..8512)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 9103..9122
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 9407..9528
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9444..9463)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9498..9517
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(9577..9593)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9577..9593)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(9590..9612)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 9601..9617
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9625..9655)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9670..9691
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(9808..9825)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9979..10567)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(10059..10078)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10738..11598)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 11361..11380
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11599..11703)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
901 atggcgtacc catacgatgt tccagattac gccaggatcc atcgatgtcg acgaattctg
961 cagatgggga tccgtcgacg gatggcgggc gtggggccgg ggggctacgc ggcggagttc
1021 gtgccaccgc cagagtgccc cgtctttgag ccgagttggg aggagttcac agatccgctc
1081 agctttatcg gccgcatccg gcctttggcg gagaaaaccg gcatctgcaa aattcggccg
1141 cccaaggact ggcagcctcc atttgcctgt gaagtaaaaa gctttcgttt cactccaaga
1201 gtccagcgcc tgaatgaact tgaggcaatg accagagtga gattggattt cttggatcaa
1261 ctagcaaaat tttgggaact tcaaggatct actctgaaga tccctgtggt agagagaaaa
1321 atcctggatc tgtatgcttt gagcaagatt gttgccagca aaggaggttt tgaaatggtc
1381 accaaagaga agaaatggtc taaagtgggt agtcgcttgg gatatctgcc aggaaaagga
1441 actgggtctc ttttgaagtc acattatgaa agaattctct acccatatga gcttttccag
1501 tctggtgtga gccttatggg tgtgcagatg cctaatttag atcttaaaga aaaagtggag
1561 cctgaggttc tcagcactga tacccaaact tccccagagc caggcacaag gatgaacatt
1621 ctgccgaaga gaacaagacg tgtgaagact cagtcagaat ctggagatgt gagtagaaac
1681 acggaactga agaaacttca gatttttggg gctgggccca aggttgtggg cttggcaatg
1741 ggaacaaaag ataaagaaga tgaggtcacc cgaagacgaa aagttaccaa caggtcagac
1801 gcatttaaca tgcaaatgag acaacggaaa ggcactctct ctgttaactt tgttgatctc
1861 tatgtttgta tgttttgtgg tcggggaaac aatgaagata aattgctttt gtgtgatgga
1921 tgtgatgaca gctatcatac attttgtcta attcctccac tacctgatgt gcccaaagga
1981 gactggaggt gtcctaaatg tgtcgccgag gaatgtagca aacctcgaga agcctttgga
2041 tttgaacaag ctgtacgaga gtatacactt cagagctttg gagagatggc agataatttt
2101 aagtctgatt attttaatat gccagtccat atggttccca cagaactagt agaaaaggaa
2161 ttttggcggc tggtaagcag cattgaagaa gatgttattg tggaatatgg agcagatatc
2221 tcctcaaaag actttggaag tggatttccg gtgaaggatg ggcggagaaa gattctgcca
2281 gaagaagagg aatatgcact ttctggttgg aatttgaata acatgcctgt cctggaacag
2341 tctgttcttg cacatattaa tgtggacatc tctggtatga aagtgccgtg gctctatgtg
2401 ggaatgtgct tctcttcttt ttgctggcac attgaggatc actggagtta ttccatcaac
2461 tacttgcact ggggggagcc aaagacatgg tatggtgtgc catctcatgc tgcagagcaa
2521 ctggaggagg tgatgagaga gctggccccc gagttatttg aatcccagcc tgatcttctg
2581 catcagttag ttaccatcat gaaccccaac gtgctaatgg agcatggtgt gcctgtgtac
2641 aggaccaatc agtgtgctgg cgagtttgtt gtgacatttc ctcgtgccta tcactctgga
2701 tttaaccagg gctacaactt tgctgaagct gtgaacttct gtactgctga ctggttgccc
2761 attggacgtc aatgtgtaaa tcattaccga cgcctaaggc gccactgtgt cttttcacac
2821 gaggaactaa ttttcaagat ggcagcagat ccagaatgct tagatgtggg gctggctgcc
2881 atggtctgca aagaattgac tctcatgact gaagaagaaa cacgattaag agagtctgtt
2941 gtacagatgg gtgtcctgat gtcagaagaa gaagtgtttg aacttgttcc tgatgatgag
3001 cggcagtgtt cagcatgcag aaccacatgt tttctctctg ctctcacatg ttcctgtaat
3061 cctgagcggc ttgtatgtct ctaccatcca actgatctgt gcccctgccc catgcagaag
3121 aaatgtctta gatatcgcta cccattagaa gacctccctt ctctgctata tggtgtaaaa
3181 gtcagggcac agtcctatga cacttgggtc agtcgtgtta cagaagcatt gtctgctaac
3241 ttcaaccaca aaaaagattt gattgaattg cgagtaatgc tggaagatgc tgaggatagg
3301 aaatacccag agaatgatct ctttcgaaaa ctcagggatg ctgtaaaaga agctgagacc
3361 tgtgcttctg tggctcagct gcttctgagc aaaaagcaga aacacagaca gagcccagat
3421 agtgggagga ctcggaccaa actgacagtg gaagaattga aggcctttgt ccaacaactt
3481 tttagtcttc cgtgtgtcat cagccaagct cggcaagtaa agaatctgct agatgatgtg
3541 gaagagtttc atgaacgtgc tcaggaggcc atgatggatg aaaccccaga ttcttccaaa
3601 ctccagatgt tgatagatat gggctctagt ctctatgtgg aactccctga attaccacga
3661 ctgaagcaag agctacaaca ggctcggtgg ttggacgaag taagactgac cttatcagat
3721 ccgcaacaag tcactttgga tgtcatgaag aagctgatag actctggggt agggttggca
3781 ccccaccatg ctgtggagaa agcaatggct gaactacagg agctccttac agtctctgaa
3841 cgatgggaag aaaaggctaa ggtctgccta caggcaagac cgaggcacag tgtggcaagt
3901 ttagaaagca ttgtgaatga agccaagaac attccagcct ttctacccaa tgtgttgtcc
3961 ttgaaagaag ccttacaaaa ggctcgagaa tggaccgcta aagtggaagc tattcagagt
4021 ggcagcaatt acgcttattt ggagcagctt gagagcttgt ctgcgaaagg acgccctatt
4081 cctgtgcgtc ttgaagcact gccgcaagtg gaatcacagg tagcagcagc acgggcatgg
4141 agagaacgga ctgggcggac gtttcttaag aagaattcta gccatacatt gttacaggtg
4201 ctgagccccc ggaccgacat tggtgtatat gggagtggca aaaataggag gaaaaaagta
4261 aaagaactaa tagaaaaaga aaaagaaaag gatctggacc tggagcctct gagtgatctg
4321 gaggaaggat tggaggaaac cagagataca gccatggtgg tggcagtttt caaagaacgg
4381 gagcaaaaag agattgaagc catgcattct ctcagagcag ccaacctagc caagatgaca
4441 atggtggacc gcatagaaga agtaaaattt tgcatttgcc gcaagacagc cagtgggttt
4501 atgctacagt gtgagctctg caaagactgg ttccataaca gctgtgttcc tcttcctaaa
4561 tcaagttccc aaaaaaaagg atccagctgg caagctaaag aagtaaaatt cctttgccct
4621 ctttgtatgc ggtctcgaag gcccaggcta gagactattc tgtcactcct ggtatccctt
4681 cagaagttgc ccgtacggtt gcctgaagga gaggccctgc agtgtttgac agaacgtgct
4741 atgagttggc aagatagagc gcggcaggct ctagccacag atgaactatc ctctgccctg
4801 gccaaactat ctgtgttgag ccagcgtatg gtggaacagg cggctcgaga aaaaactgaa
4861 aagatcatca gtgcagaact ccaaaaagca gctgccaatc cagacttaca gggacactta
4921 cctagtttcc agcagtctgc ttttaaccgg gtggtgagca gtgtgtcatc ttctcctcga
4981 caaacaatgg actatgatga tgaagaaaca gactctgatg aagacattcg agagacatat
5041 ggctacgaca tgaaggacac agccagtgtg aagtcctcta gtagtcttga acccaatctt
5101 ttttgtgatg aagagattcc catcaaatcc gaggaggtgg tgacccacat gtggacagca
5161 ccttcatttt gtgcagagca tgcttattct tctgcttcta agagttgttc tcaaggttct
5221 agcaccccaa ggaaacaacc tcggaagagc cctttggtgc cccgaagttt ggaacctcca
5281 gtgctggagt tgtcacctgg agctaaggca caactggaag aacttatgat ggttggagat
5341 ctcctggaag tatctctgga cgagactcaa cacatatggc ggattttgca ggccacacac
5401 ccaccctctg aagacagatt cttgcatatc atggaggatg acagcatgga agagaaacca
5461 ctaaaagtga aaggaaagga ctcttcagag aagaaacgga aacggaagct agaaaaggta
5521 gagcaacttt ttggagaagg aaaacagaag tccaaggagt taaagaaaat ggacaaacct
5581 agaaagaaga aattaaaatt aggtgcagac aaatcaaagg agctgaataa actggccaag
5641 aaactagcaa aagaagaaga gagaaagaaa aagaaggaga aggctgctgc agccaaagtt
5701 gaacttgtga aagagagcac tgaaaagaaa agagagaaaa aggtgctgga catcccctca
5761 aagtatgact ggtcaggagc agaggagtct gatgatgaga atgctgtgtg cgcagcacag
5821 aactgccaaa ggccctgcaa ggacaaggga gttgtatttg taacggaaga agagaagaat
5881 aaaaaatatt agttttaaaa gtgtcctatg tgactgcttt tctaaaaagg tagactgggt
5941 acaatgtgat ggtggctgtg atgagtggtt tcatcaagtt tgtgtgggtg tatctccaga
6001 aatggctgaa aatgaagatt acatctgtat aaactgtgca aagaagcagg ggccagttag
6061 cccaggtcca gcaccacctc cttccttcat aatgagctac aaactaccaa tggaggatct
6121 taaagagacc agttagcaga tgcttggtta gtttgggaca tggggggaca tggaccacat
6181 tgagacctta gtcatcaagt agagtggttt atatcacttg gaatgttgct tctaaagatg
6241 aatggccttc agagaaagtc cccttagtgc tggcttcctc tttgcatgga ctctgtgggt
6301 tacattgctc tatcaacata tctatgcaga gggtgtcttc tttggtacaa cagccaatat
6361 ctcatgtctc ctttgagtgt ggtttactgc attaaggcca gatgcttaat tgagctctag
6421 ggtggctggt tagtattaat acattggtgt gctaacaggg catataggat gtggcttttg
6481 tccagctgat agtagttaga ggcttacaac ttaggagcag caccaactga aggtgctaat
6541 tgcttggatc tccttcatta ggatagttgg agagggattg gagtaccact ttcttccact
6601 gttaccaggt acttaatgcc ctaaagatac aactaggagt aacagggcca aagttatttc
6661 tgttagacgt caaggaatgg tatcacagtc tattgacctc agcgatttgt gcttgtttgt
6721 gctagaagaa catcccaaat aggagaacct ctcacaagct ggggcaggtc accttatctt
6781 tgtaagatga ggatatcatc tagatcagaa atctgactag attggattct gaggagaaga
6841 acctactaca aggcaaggag ccgttttttg gctttgaaaa gtcttgctgt cttgggtcta
6901 cattttaggg aagagcaggt acatggatcc aggcttctgc caaaaaaaaa aagagaagaa
6961 gatgacgatg atgaccagtc gtactatctt actgagccac agtgatgcat gcttttcggg
7021 gaaaacttca ttcacaagta ttccagacac caggcttcag gcatggccat gagcaagacc
7081 agcaaataac agctttttcc cttgcagccc tgaccccaat gtctgctgtt tccaacactg
7141 gtgatttcta actacggccc acagcagatg ctgttgaata acaccatggc ttcatcagag
7201 gatgtggggt tgtagtacct ctgggtgatg aagttgtttt agcaaatcca tttttaaaaa
7261 aaaaaaaaaa tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat
7321 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
7381 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
7441 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
7501 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
7561 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
7621 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
7681 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
7741 aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc
7801 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
7861 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
7921 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg
7981 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
8041 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa
8101 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
8161 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
8221 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
8281 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
8341 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
8401 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
8461 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
8521 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
8581 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
8641 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
8701 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
8761 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
8821 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
8881 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
8941 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
9001 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
9061 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
9121 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
9181 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
9241 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
9301 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
9361 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
9421 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
9481 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
9541 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
9601 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
9661 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
9721 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
9781 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
9841 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
9901 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
9961 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
10021 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
10081 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
10141 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
10201 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
10261 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
10321 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
10381 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
10441 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
10501 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
10561 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
10621 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
10681 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
10741 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
10801 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
10861 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
10921 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
10981 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
11041 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
11101 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
11161 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
11221 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
11281 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
11341 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
11401 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
11461 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
11521 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
11581 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
11641 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
11701 gcgcacattt ccccgaaaag tgccacctga cgtc
//
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