| 质粒名称: | pGEX-2T-AT5G43960 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000926 |
| 产品用途: | Express proteins in vitro |
| 存储实验室: | Peiguo YANG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pGEX-2T |
| 表达系统: | 细菌表达 |
| 启动子: | tac, AmpR, Lac, LacIq |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素氯霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha, RosettaDE3, BL21 |
|
插入基因信息 |
|
| 插入基因名称: | AT5G43960 |
| 物种来源: | Arabidopsis thaliana |
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
The divergent effects of G3BP orthologs on human stress granule assembly imply a centric role for the core protein interaction network
Yao Z., Liu Y., Chen Q., Chen X., Zhu Z., Song S., Ma X., Yang P.
Cell Rep. 43, 114617 (2024)
|
买家导航
pGEX-2T-AT5G43960载体质粒基本信息
pGEX-2T-AT5G43960质粒图谱载体图谱和pGEX-2T-AT5G43960载体序列质粒序列多克隆位点信息
pGEX-2T-AT5G43960质粒载体简介
pGEX-2T-AT5G43960质粒序列载体序列
LOCUS pGEX-2T-AT5G43960 6303 bp DNA circular SYN 08-APR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6303)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Apr 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6303
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 183..211
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 219..235
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 258..911
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/label=GST
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
CDS 918..938
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQG"
CDS 942..2294
/codon_start=1
/label=AT5G43960
/translation="MATPYPGATQVGSYFVGQYYQVLQQQPDLIHQFYSEPSRAIRIDG
DSTETANSLLHIHNMVMSLNFTAIEVKTINSVESWEGGVLVVVSGSVKTKEFSNRRSFV
QTFFLAPQEKGYFVLSDVFLFVDEGTVYYHQPSYLSEIKHEAQLNPPTRHPDPQVSDYV
LEEEASDYVNAVQIKDDLVDKYSLQEDQHQPQHEDYEDEVAIEETPREEVAVDVVHEHR
AAPVEEPVGEKSKMSYASILKVAKEAATVPVAATQPSYNKSSQDINEWDQPMRTPSPQL
AAPLAPIQQSNSSTYVSDYGAEAEDGSGFEDFEFKSVYVRNLPSDISASEIEEEFKNFG
TIKPDGVFLRTRKDVMGVCYAFVEFEDMTSVENAIKASPIYLGGRQVYIEERRPNPAGV
RGARRGGGRGRGGYPTEAPRGRFGGRGSGRGNQDGGDYRPRGNGYYRGGGR"
promoter 2606..2710
/gene="bla"
/label=AmpR promoter
CDS 2711..3571
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3742..4330
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 4574..4651
/gene="lacI (mutant)"
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
CDS 4652..5734
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
protein_bind 5747..5768
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5783..5813
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5821..5837
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5845..5861
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 5857..6303
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGI"
primer_bind complement(5877..5893)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
901 atcctccaaa atcggatgaa aacctgtatt ttcagggatc catggcaacc ccgtatccgg
961 gcgcaaccca ggtgggcagc tattttgttg gtcagtatta tcaggtgctg cagcagcagc
1021 cggatctgat tcatcagttt tatagtgaac cgagccgcgc aattcgcatt gatggtgaca
1081 gtaccgaaac cgccaatagt ctgctgcata ttcataatat ggttatgagt ctgaacttca
1141 ccgccattga agttaaaacc attaatagcg ttgagagctg ggaaggcggt gtgctggtgg
1201 ttgtgagcgg tagtgtgaaa accaaagaat tttctaatcg tcgtagtttt gtgcagacct
1261 ttttcctggc accgcaggaa aaaggttatt ttgttctgag tgatgtgttt ctgtttgttg
1321 atgaaggcac cgtgtattat catcagccga gctatctgag tgaaattaag catgaagcac
1381 agctgaatcc gccgacccgt catccggacc ctcaggtgag tgattatgtg ctggaagaag
1441 aagcaagcga ttatgtgaat gccgttcaga ttaaggatga tctggttgat aaatatagcc
1501 tgcaggaaga tcagcatcag ccgcagcatg aagattatga agatgaagtt gcaattgaag
1561 aaaccccgcg tgaagaagtt gccgttgatg ttgtgcatga acatcgcgca gccccggttg
1621 aagaaccggt tggcgaaaaa agcaaaatga gttatgccag cattctgaaa gtggccaaag
1681 aagccgccac cgtgccggtt gcagcaaccc agccgagtta taataagagc agccaggata
1741 ttaatgaatg ggatcagccg atgcgcaccc cgagtccgca gctggcagcc cctctggctc
1801 cgattcagca gagcaatagc agtacctatg ttagtgatta tggtgcagaa gcagaagatg
1861 gcagcggctt tgaagatttt gagtttaaaa gtgtgtacgt tcgcaatctg ccgagtgata
1921 ttagtgcaag cgaaattgaa gaagagttta aaaatttcgg caccattaag ccggatggtg
1981 tgtttctgcg tacccgtaaa gatgttatgg gtgtgtgtta tgcatttgtg gaatttgaag
2041 atatgaccag tgtggaaaat gccattaagg ccagtccgat ctatctgggt ggtcgtcagg
2101 tgtatattga agaacgtcgc ccgaatccgg caggtgttcg tggcgcccgc cgtggtggtg
2161 gtcgtggtag aggtggttat ccgaccgaag ccccgcgcgg tcgttttggc ggccgtggta
2221 gtggtcgtgg caatcaggat ggcggcgatt atcgtccgcg tggtaatggt tattatcgtg
2281 gcggtggtcg ctgagaattc atcgtgactg actgacgatc tgcctcgcgc gtttcggtga
2341 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
2401 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
2461 cgcagccatg acccagtcac gtagcgatag cggagtgtat aattcttgaa gacgaaaggg
2521 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
2581 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
2641 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
2701 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
2761 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
2821 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
2881 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
2941 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
3001 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
3061 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
3121 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
3181 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
3241 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
3301 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
3361 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
3421 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
3481 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
3541 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
3601 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
3661 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
3721 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
3781 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
3841 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
3901 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
3961 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
4021 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
4081 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
4141 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
4201 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
4261 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
4321 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
4381 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
4441 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
4501 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
4561 ccgcataaat tccgacacca tcgaatggtg caaaaccttt cgcggtatgg catgatagcg
4621 cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc
4681 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt
4741 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa
4801 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag
4861 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact
4921 gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc
4981 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga
5041 ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt
5101 ctctgaccag acacccatca acagtattat tttctcccat gaagacggta cgcgactggg
5161 cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag
5221 ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat
5281 tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat
5341 gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc
5401 gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt
5461 agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa
5521 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg
5581 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct
5641 ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
5701 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
5761 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
5821 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac ggattcactg
5881 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
5941 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
6001 tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa
6061 gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc
6121 tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtaac ctatcccatt
6181 acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt
6241 aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttgga
6301 att
//
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