pYSJ51

LOCUS       pYSJ51               6464 bp DNA     circular SYN 08-APR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     215..274
                     /label=oYSJ167-X7-F
     misc_feature    234..243
                     /label=(CCGGAA)X1
     misc_feature    244..251
                     /label=X2
     misc_feature    252..259
                     /label=X3
     primer_bind     complement(255..310)
                     /label=oYSJ168-X7-R
     misc_feature    260..275
                     /label=X5
     misc_feature    276..291
                     /label=X7
     misc_feature    298..445
                     /label=hcmvmin
     misc_feature    367..381
                     /label=New Feature
     misc_feature    468..491
                     /label=New Feature
     misc_feature    493..2013
                     /label=SEAP
     terminator      2061..2288
                     /label=bGH PA
     misc_feature    2956..3033
                     /label=SV40 ORI
     CDS             3175..3966
                     /label=Kan/neoR
     primer_bind     complement(4306..4326)
                     /label=M13-rev
     misc_binding    complement(4332..4354)
                     /label=LacO
     promoter        complement(4359..4388)
                     /label=lac
     CDS             complement(5471..6130)
                     /label=AmpR
     promoter        complement(6370..6398)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtccccgga
      241 agtccggaag tccggaagtc cggaagtccg gaagtccgga agtccggaag tgctagctcg
      301 agctcggtac ccgggtcgag taggcgtgta cggtgggagg cctatataag cagagctcgt
      361 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      421 caccgggacc gatccagcct ccgcggcccc gaattcgagc tcgcccgggg atcctctaga
      481 gtcagcttct gcatgctgct gctgctgctg ctgctgggcc tgaggctaca gctctccctg
      541 ggcatcatcc cagttgagga ggagaacccg gacttctgga accgcgaggc agccgaggcc
      601 ctgggtgccg ccaagaagct gcagcctgca cagacagccg ccaagaacct catcatcttc
      661 ctgggcgatg ggatgggggt gtctacggtg acagctgcca ggatcctaaa agggcagaag
      721 aaggacaaac tggggcctga gatacccctg gccatggacc gcttcccata tgtggctctg
      781 tccaagacat acaatgtaga caaacatgtg ccagacagtg gagccacagc cacggcctac
      841 ctgtgcgggg tcaagggcaa cttccagacc attggcttga gtgcagccgc ccgctttaac
      901 cagtgcaaca cgacacgcgg caacgaggtc atctccgtga tgaatcgggc caagaaagca
      961 gggaagtcag tgggagtggt aaccaccaca cgagtgcagc acgcctcgcc agccggcacc
     1021 tacgcccaca cggtgaaccg caactggtac tcggacgccg acgtgcctgc ctcggcccgc
     1081 caggaggggt gccaggacat cgctacgcag ctcatctcca acatggacat tgacgtgatc
     1141 ctaggtggag gccgaaagta catgtttccc atgggaaccc cagaccctga gtacccagat
     1201 gactacagcc aaggtgggac caggctggac gggaagaatc tggtgcagga atggctggcg
     1261 aagcgccagg gtgcccggta tgtgtggaac cgcactgagc tcatgcaggc ttccctggac
     1321 ccgtctgtga cccatctcat gggtctcttt gagcctggag acatgaaata cgagatccac
     1381 cgagactcca cactggaccc ctccctgatg gagatgacag aggctgccct gcgcctgctg
     1441 agcaggaacc cccgcggctt cttcctcttc gtggagggtg gtcgcatcga ccatggtcat
     1501 catgaaagca gggcttaccg ggcactgact gagacgatca tgttcgacga cgccattgag
     1561 agggcgggcc agctcaccag cgaggaggac acgctgagcc tcgtcactgc cgaccactcc
     1621 cacgtcttct ccttcggagg ctaccccctg cgagggagct ccatcttcgg gctggcccct
     1681 ggcaaggccc gggacaggaa ggcctacacg gtcctcctat acggaaacgg tccaggctat
     1741 gtgctcaagg acggcgcccg gccggatgtt accgagagcg agagcgggag ccccgagtat
     1801 cggcagcagt cagcagtgcc cctggacgaa gagacccacg caggcgagga cgtggcggtg
     1861 ttcgcgcgcg gcccgcaggc gcacctggtt cacggcgtgc aggagcagac cttcatagcg
     1921 cacgtcatgg ccttcgccgc ctgcctggag ccctacaccg cctgcgacct ggcgcccccc
     1981 gccggcacca ccgacgccgc gcacccgggt taagcggccg ctcgagtcta gagggcccgt
     2041 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     2101 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     2161 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     2221 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     2281 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     2341 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     2401 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     2461 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     2521 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     2581 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     2641 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     2701 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     2761 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     2821 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     2881 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     2941 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3001 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     3061 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     3121 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     3181 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     3241 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     3301 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     3361 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     3421 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     3481 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     3541 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     3601 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     3661 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     3721 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     3781 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     3841 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     3901 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     3961 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     4021 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     4081 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     4141 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4201 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4261 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     4321 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4381 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     4441 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     4501 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     4561 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     4621 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     4681 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4741 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4801 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4861 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4921 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4981 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5041 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5101 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     5161 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5221 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca
     5281 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     5341 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     5401 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     5461 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     5521 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     5581 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     5641 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     5701 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     5761 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     5821 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     5881 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     5941 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6001 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6061 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     6121 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6181 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6241 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6301 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     6361 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     6421 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//