| 质粒名称: |
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL4A5-FLAG
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001897 |
| 产品用途: | 3' AAVLINK plasmid for COL4A5 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | COL4A5 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL4A5-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL4A5-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL4A5-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL4A5-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL4A5-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL4A5-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL4A5-FLAG 6792 bp DNA circular SYN 25-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6792)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6792
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3505
/codon_start=1
/gene="COL4A5"
/gene_synonym="ASLN; ATS; ATS1; CA54"
/product="collagen alpha-5(IV) chain isoform 2 precursor"
/label=COL4A5
/note="isoform 2 precursor is encoded by transcript
variant 2; collagen of basement membrane, alpha-5 chain;
collagen IV, alpha-5 polypeptide; collagen alpha-5(IV)
chain; dA149D17.3; collagen, type IV, alpha 5; dA24A23.1"
/db_xref="CCDS:CCDS35366.1"
/db_xref="GeneID:1287"
/db_xref="HGNC:HGNC:2207"
/db_xref="MIM:303630"
/protein_id="NP_203699.1"
/translation="GVEGPPGPSGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKG
EPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNP
GLPGEPGPVGGGGHPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLS
GQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPP
GRPGPTGFQGLPGPEGPPGLPGNGGIKGEKGNPGQPGLPGLPGLKGDQGPPGLQGNPGR
PGLNGMKGDPGLPGVPGFPGMKGPSGVPGSAGPEGEPGLIGPPGPPGLPGPSGQSIIIK
GDAGPPGIPGQPGLKGLPGPQGPQGLPGPTGPPGDPGRNGLPGFDGAGGRKGDPGLPGQ
PGTRGLDGPPGPDGLQGPPGPPGTSSVAHGFLITRHSQTTDAPQCPQGTLQVYEGFSLL
YVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSM
QPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAE
GSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLK
AGDLRTRISRCQVCMKRT"
misc_feature 1559..1564
/label=SDS
misc_feature 1817..1822
/label=SDS
misc_feature 1844..1849
/label=SDS
misc_feature 2147..2152
/label=SDS
misc_feature 2207..2212
/label=SDS
misc_feature 2522..2527
/label=SDS
misc_feature 2707..2712
/label=SDS
misc_feature 2814..2819
/label=SDS
CDS 3512..3577
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3581..4012
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3725..3730
/label=SDS
repeat_region 4055..4195
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4055..4184
/label=AAV2 ITR
repeat_region 4056..4196
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4270..4725
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5007..5111
/gene="bla"
/label=AmpR promoter
CDS 5112..5972
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6143..6731
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggtg tggaagggcc
1561 tcctggacct tctggagttc ctggacaacc tggctcccca ggattacctg gacagaaagg
1621 cgacaaaggt gatcctggta tttcaagcat tggtcttcca ggtcttcctg gtccaaaggg
1681 tgagcctggt ctgcctggat acccagggaa ccctggtatc aaaggttctg tgggagatcc
1741 tggtttgccc ggattaccag gaacccctgg agcaaaagga caaccaggcc ttcctggatt
1801 cccaggaacc ccaggccctc ctggaccaaa aggtattagt ggccctcctg ggaaccccgg
1861 ccttccagga gaacctggtc ctgtaggtgg tggaggtcat cctgggcaac cagggcctcc
1921 aggcgaaaaa ggcaaacccg gtcaagatgg tattcctgga ccagctggac agaagggtga
1981 accaggtcaa ccaggctttg gaaacccagg accccctgga cttccaggac tttctggcca
2041 aaagggtgat ggaggattac ctgggattcc aggaaatcct ggccttccag gtccaaaggg
2101 cgaaccaggc tttcacggtt tccctggtgt gcagggtccc ccaggccctc ctggttctcc
2161 gggtccagct ctggaaggac ctaaaggcaa ccctgggccc caaggtcctc ctgggagacc
2221 aggtcctaca ggttttcaag gtctaccagg tccagaaggt cctccaggtc tccctggaaa
2281 tggaggtatt aaaggagaga agggaaatcc aggccaacct gggctacctg gcttgcctgg
2341 tttgaaagga gatcaaggac caccaggact ccagggtaat cctggccggc cgggtctcaa
2401 tggaatgaaa ggagatcctg gtctccctgg tgttccagga ttcccaggca tgaaaggacc
2461 cagtggagta cctggatcag ctggccctga gggggaaccg ggacttattg gtcctccagg
2521 tcctcctgga ttacctggtc cttcaggaca gagtatcata attaaaggag atgctggtcc
2581 tccaggaatc cctggccagc ctgggctaaa gggtctacca ggaccccaag gacctcaagg
2641 cttaccaggt ccaactggcc ctccaggaga tcctggacgc aatggactcc ctggctttga
2701 tggtgcagga gggcgcaaag gagacccagg tctgccagga cagccaggta cccgtggttt
2761 ggatggtccc cctggtccag atggattgca aggtccccca ggtccccctg gaacctcctc
2821 tgttgcacat ggatttctta ttacacgcca cagccagaca acggatgcac cacaatgccc
2881 acagggaaca cttcaggtct atgaaggctt ttctctcctg tatgtacaag gaaataaaag
2941 agcccacggt caagacttgg ggacggctgg cagctgcctt cgtcgcttta gtaccatgcc
3001 tttcatgttc tgcaacatca ataatgtttg caactttgct tcaagaaatg actattctta
3061 ctggctctct accccagagc ccatgccaat gagcatgcaa cccctaaagg gccagagcat
3121 ccagccattc attagtcgat gtgcagtatg tgaagctcca gctgtggtga tcgcagttca
3181 cagtcagacg atccagattc cccattgtcc tcagggatgg gattctctgt ggattggtta
3241 ttccttcatg atgcatacaa gtgcaggggc agaaggctca ggtcaagccc tagcctcccc
3301 tggttcctgc ttggaagagt ttcgttcagc tcccttcatc gaatgtcatg ggaggggtac
3361 ctgtaactac tatgccaact cctacagctt ttggctggca actgtagatg tgtcagacat
3421 gttcagtaaa cctcagtcag aaacgctgaa agcaggagac ttgaggacac gaattagccg
3481 atgtcaagtg tgcatgaaga ggacatgtac agactacaaa gaccatgacg gtgattataa
3541 agatcatgac atcgactaca aggatgacga tgacaagtaa ctcgagataa tcaacctctg
3601 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
3661 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
3721 ttctcctcct tgtataaatc ctggttagtt cttgccacgg cggaactcat cgccgcctgc
3781 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgtttatt
3841 tgtgaaattt gtgatgctat tgctttattt gtaaccatct agctttattt gtgaaatttg
3901 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
3961 ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagatctgg
4021 agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc
4081 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
4141 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
4201 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
4261 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
4321 gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct
4381 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
4441 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
4501 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
4561 ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt
4621 ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac
4681 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
4741 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
4801 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
4861 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
4921 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
4981 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
5041 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
5101 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
5161 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
5221 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
5281 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
5341 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
5401 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
5461 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
5521 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
5581 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
5641 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
5701 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
5761 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
5821 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
5881 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
5941 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
6001 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
6061 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
6121 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
6181 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
6241 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
6301 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
6361 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
6421 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
6481 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
6541 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
6601 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
6661 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
6721 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
6781 ttgctcacat gt
//
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pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL4A5-FLAG质粒载体应用举例
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