pSL1968

LOCUS       pSL1968              6541 bp DNA     circular SYN 09-MAR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Aug 31, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    241..260
                     /label=C120
     misc_feature    266..285
                     /label=C120
     misc_feature    291..310
                     /label=C120
     misc_feature    316..335
                     /label=C120
     misc_feature    341..360
                     /label=C120
     promoter        373..404
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    432..436
                     /label=NheI
     primer_bind     433..468
                     /label=Oscl31-3
     misc_feature    447..451
                     /label=New Feature
     primer_bind     454..468
                     /label=oSCL775
     primer_bind     456..468
                     /label=oSCL456
     misc_feature    462..2021
                     /label=SEAP
     primer_bind     complement(650..686)
                     /label=oSCL545
     primer_bind     667..695
                     /label=oSCL544
     primer_bind     complement(1483..1514)
                     /label=oSCL546
     primer_bind     1495..1527
                     /label=oSCL547
     primer_bind     1580..1610
                     /label=Oscl50-1
     primer_bind     complement(1580..1610)
                     /label=Oscl50-2
     primer_bind     complement(1969..2021)
                     /label=oSCL795
     primer_bind     complement(2003..2018)
                     /label=oSCL475
     primer_bind     complement(2005..2061)
                     /label=oSCL548
     primer_bind     complement(2005..2047)
                     /label=oSCL083
     primer_bind     complement(2005..2021)
                     /label=Oscl31-4
     primer_bind     complement(2005..2021)
                     /label=oSCL457
     primer_bind     complement(2005..2021)
                     /label=oSCL776
     misc_feature    2065..2070
                     /label=EcoRI
     misc_feature    2104..2109
                     /label=New Feature
     terminator      2138..2365
                     /label=bGH PA
     rep_origin      2411..2839
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2853..3182
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3249..4043
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4217..4338
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4383..4403)
                     /label=M13-rev
     misc_binding    complement(4409..4431)
                     /label=LacO
     promoter        complement(4435..4465)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4480..4501
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4789..5374)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5545..6405)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6406..6510)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtcctagag
      241 taggtagcct ttagtccatg cgttataggt agcctttagt ccatgcgtta taggtagcct
      301 ttagtccatg cgttataggt agcctttagt ccatgcgtta taggtagcct ttagtccatg
      361 accggtagac actagagggt atataatgga agctcgactt ccagcttggc aatccggtac
      421 tgttggtaaa gctagcgttt aaacttaagc ttagcgccac catgctgctg ctgctgctgc
      481 tgctgggcct gaggctacag ctctccctgg gcatcatccc agttgaggag gagaacccgg
      541 acttctggaa ccgcgaggca gccgaggccc tgggtgccgc caagaagctg cagcctgcac
      601 agacagccgc caagaacctc atcatcttcc tgggcgatgg gatgggggtg tctacggtga
      661 cagctgccag aatcctaaaa gggcagaaga aggacaaact ggggcctgag atacccctgg
      721 ccatggaccg cttcccatat gtggctctgt ccaagacata caatgtagac aaacatgtgc
      781 cagacagtgg agccacagcc acggcctacc tgtgcggggt caagggcaac ttccagacca
      841 ttggcttgag tgcagccgcc cgctttaacc agtgcaacac gacacgcggc aacgaggtca
      901 tctccgtgat gaatcgggcc aagaaagcag ggaagtcagt gggagtggta accaccacac
      961 gagtgcagca cgcctcgcca gccggcacct acgcccacac ggtgaaccgc aactggtact
     1021 cggacgccga cgtgcctgcc tcggcccgcc aggaggggtg ccaggacatc gctacgcagc
     1081 tcatctccaa catggacatt gacgtgatcc taggtggagg ccgaaagtac atgtttcgca
     1141 tgggaacccc agaccctgag tacccagatg actacagcca aggtgggacc aggctggacg
     1201 ggaagaatct ggtgcaggaa tggctggcga agcgccaggg tgcccggtat gtgtggaacc
     1261 gcactgagct catgcaggct tccctggacc cgtctgtgac ccatctcatg ggtctctttg
     1321 agcctggaga catgaaatac gagatccacc gagactccac actggacccc tccctgatgg
     1381 agatgacaga ggctgccctg cgcctgctga gcaggaaccc ccgcggcttc ttcctcttcg
     1441 tggagggtgg tcgcatcgac catggtcatc atgaaagcag ggcttaccgg gcactgactg
     1501 agaccatcat gttcgacgac gccattgaga gggcgggcca gctcaccagc gaggaggaca
     1561 cgctgagcct cgtcactgcc gaccactccc acgtgttctc cttcggaggc taccccctgc
     1621 gagggagctc catcttcggg ctggcccctg gcaaggcccg ggacaggaag gcctacacgg
     1681 tcctcctata cggaaacggt ccaggctatg tgctcaagga cggcgcccgg ccggatgtta
     1741 ccgagagcga gagcgggagc cccgagtatc ggcagcagtc agcagtgccc ctggacgaag
     1801 agacccacgc aggcgaggac gtggcggtgt tcgcgcgcgg cccgcaggcg cacctggttc
     1861 acggcgtgca ggagcagacc ttcatagcgc acgtcatggc cttcgccgcc tgcctggagc
     1921 cctacaccgc ctgcgacctg gcgccccccg ccggcaccac cgacgccgcg cacccgggtt
     1981 acagcagagt cggggcggcc ggccgcttcg agcagacatg agcggccgct caatctcgag
     2041 gggatccact agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgctc
     2101 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     2161 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     2221 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     2281 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     2341 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     2401 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     2461 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     2521 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     2581 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     2641 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     2701 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     2761 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     2821 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     2881 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     2941 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3001 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     3061 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     3121 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     3181 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     3241 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     3301 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     3361 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     3421 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     3481 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     3541 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     3601 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     3661 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     3721 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     3781 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     3841 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     3901 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     3961 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     4021 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     4081 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     4141 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     4201 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     4261 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     4321 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     4381 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     4441 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     4501 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     4561 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     4621 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     4681 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     4741 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4801 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4861 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4921 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4981 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     5041 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     5101 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     5161 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5221 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5281 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa
     5341 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5401 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     5461 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     5521 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     5581 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     5641 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     5701 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     5761 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     5821 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     5881 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     5941 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     6001 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     6061 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     6121 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     6181 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     6241 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     6301 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     6361 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     6421 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     6481 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     6541 c
//