| 质粒名称: | lentiGuide-Puro |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 52963 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | Custom |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 9482 bp |
| 载体类型: | 哺乳细胞表达,慢病毒载体,CRISPR |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | S. pyogenes sgRNA cassette |
| 物种来源: | 合成 |
| 插入大小: | 100 bp |
| 启动子 | hU6 |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BsmBI(未破坏) |
| 3' 克隆位点: | BsmBI (未破坏) |
| 5' 测序引物 | hU6-F |
| 3' 测序引物 | hGata4-rev (5'-ATTGTGGATGAATACTGCC-3') |
|
文献引用方法 |
|
| 材料和方法部分: | lentiGuide-Puro was a gift from Feng Zhang (Addgene plasmid # 52963) |
| 参考文献部分: | Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. 10.1038/nmeth.3047 PubMed 25075903 |
买家导航
lentiGuide-Puro载体质粒基本信息
lentiGuide-Puro质粒图谱载体图谱和lentiGuide-Puro载体序列质粒序列多克隆位点信息
lentiGuide-Puro质粒载体简介
lentiGuide-Puro质粒序列载体序列
LOCUS lentiGuide-Puro Sequencing Result 10183 bp DNA SYN
DEFINITION lentiGuide-Puro Sequencing Result
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..10183
/organism="lentiGuide-Puro Sequencing Result"
/mol_type="other DNA"
promoter 171..403
/label="hU6_promoter"
misc_feature 333..352
/label="LKO1_5_primer"
misc_feature 2427..2442
/label="cPPT"
promoter 2686..3857
/label="EF1a_promoter"
CDS complement(2977..3597)
/label="ORF frame 2"
misc_feature 3805..3825
/label="EF1a_fwd_primer"
gene 3870..4469
/label="puro (variant)"
/gene="puro (variant)"
CDS 3870..4469
/label="ORF frame 3"
misc_feature 4487..5062
/label="WPRE"
CDS 4575..5084
/label="ORF frame 3"
CDS 4588..5118
/label="ORF frame 1"
misc_feature 5128..5143
/label="cPPT"
misc_feature 5128..5149
/label="U3PPT"
misc_feature 5145..5197
/label="delta_U3"
misc_feature 5198..5378
/label="truncHIV-1_3_LTR"
misc_feature 5198..5378
/label="HIV-1_5_LTR"
terminator 5459..5578
/label="SV40_PA_terminator"
misc_feature 5547..5566
/label="EBV_rev_primer"
rep_origin 5596..5694
/label="SV40_origin"
misc_feature 5679..5698
/label="SV40pro_F_primer"
promoter complement(5773..5791)
/label="T7_promoter"
promoter complement(5801..5817)
/label="M13_forward20_primer"
misc_feature complement(5810..5832)
/label="M13_pUC_fwd_primer"
misc_feature 5810..5958
/label="lacZ_a"
rep_origin 5976..6282
/label="f1_origin"
promoter 6475..6503
/label="AmpR_promoter"
gene 6545..7405
/label="Ampicillin"
/gene="Ampicillin"
CDS 6545..7405
/label="ORF frame 2"
rep_origin 7560..8179
/label="pBR322_origin"
promoter 8488..8517
/label="lac_promoter"
misc_feature 8531..8553
/label="M13_pUC_rev_primer"
promoter 8552..8570
/label="M13_reverse_primer"
promoter 8587..8606
/label="T3_promoter"
misc_feature 8860..9040
/label="HIV-1_5_LTR"
misc_feature 8860..9040
/label="truncHIV-1_3_LTR"
misc_feature 9151..9195
/label="HIV-1_psi_pack"
CDS 9583..275
/label="ORF frame 1"
misc_feature 9705..9938
/label="RRE"
ORIGIN
1 TAACAAATTG GCTGTGGTAT ATAAAATTAT TCATAATGAT AGTAGGAGGC TTGGTAGGTT
61 TAAGAATAGT TTTTGCTGTA CTTTCTATAG TGAATAGAGT TAGGCAGGGA TATTCACCAT
121 TATCGTTTCA GACCCACCTC CCAACCCCGA GGGGACCCAG AGAGGGCCTA TTTCCCATGA
181 TTCCTTCATA TTTGCATATA CGATACAAGG CTGTTAGAGA GATAATTAGA ATTAATTTGA
241 CTGTAAACAC AAAGATATTA GTACAAAATA CGTGACGTAG AAAGTAATAA TTTCTTGGGT
301 AGTTTGCAGT TTTAAAATTA TGTTTTAAAA TGGACTATCA TATGCTTACC GTAACTTGAA
361 AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAACACC GGAGACGGTT
421 GTAAATGAGC ACACAAAATA CACATGCTAA AATATTATAT TCTATGACCT TTATAAAATC
481 AACCAAAATC TTCTTTTTAA TAACTTTAGT ATCAATAATT AGAATTTTTA TGTTCCTTTT
541 TGCAAACTTT TAATAAAAAT GAGCAAAATA AAAAAACGCT AGTTTTAGTA ACTCGCGTTG
601 TTTTCTTCAC CTTTAATAAT AGCTACTCCA CCACTTGTTC CTAAGCGGTC AGCTCCTGCT
661 TCAATCATTT TTTGAGCATC TTCAAATGTT CTAACTCCAC CAGCTGCTTT AACTAAAGCA
721 TTGTCTTTAA CAACTGACTT CATTAGTTTA ACATCTTCAA ATGTTGCACC TGATTTTGAA
781 AATCCTGTTG ATGTTTTAAC AAATTCTAAT CCAGCTTCAA CAGCTATTTC ACAAGCTTTC
841 ATGATTTCTT CTTTTGTTAA TAAACAATTT TCCATAATAC ATTTAACAAC ATGTGATCCA
901 GCTGCTTTTT TTACAGCTTT CATGTCTTCT AAAACTAATT CATAATTTTT GTCTTTTAAT
961 GCACCAATAT TTAATACCAT ATCAATTTCT GTTGCACCAT CTTTAATTGC TTCAGAAACT
1021 TCGAATGCTT TTGTAGCTGT TGTGCATGCA CCTAGAGGAA AACCTACAAC ATTTGTTATT
1081 CCTACATTTG TGCCTTTTAA TAATTCTTTA CAATAGCTTG TTCAATATGA ATTAACACAA
1141 ACTGTTGCAA AATCAAATTC AATTGCTTCA TCACATAATT GTTTAATTTC AGCTTTCGTA
1201 GCATCTTGTT TTAATAATGT GTGATCTATA TATTTGTTTA GTTTCATTTT TTCTCCTATA
1261 TATTCATTTT TAATTTTAAT TCTTTAATAA TTTCGTCTAC TTTAACTTTA GCGTTTTGAA
1321 CAGATTCACC AACACCTATA AAATAAATTT TTAGTTTAGG TTCAGTTCCA CTTGGGCGAA
1381 CAGCAAATCA TGACTTATCT TCTAAATAAA ATTTTAGTAA GTCTTGTCCT GGCATATTAT
1441 ACATTCCATC GATGTAGTCT TCAACATTAA CAACTTTAAG TCCAGCAATT TGAGTTAAGG
1501 GTGTTGCTCT CAATGATTTC ATTAATGGTT CAATTTTTAA TTTCTTTTCT TCTGGTTTAA
1561 AATTCAAGTT TAAAGTGAAA GTGTAATATG CACCCATTTC TTTAAATAAA TCTTCTAAAT
1621 AGTCTACTAA TGTTTTATTT TGTTTTTTAT AAAATCAAGC AGCCTCTGCT ATTAATATAG
1681 AAGCTTGTAT TCCATCTTTA TCTCTAGCTG AGTCATCAAT TACATATCCA TAACTTTCTT
1741 CATAAGCAAA AACAAAATTT AATCCGTTAT CTTCTTCTTT AGCAATTTCT CTACCCATTC
1801 ATTTAAATCC AGTTAAAGTT TTTACAATAT TAACTCCATA TTTTTCATGA GCGATTCTAT
1861 CACCCAAATC ACTTGTTACA AAACTTGAAT ATAGAGCCGG ATTTTTTGGA ATGCTATTTA
1921 AGCGTTTTAG ATTTGATAAT TTTCAATCAA TTAAAATTGG TCCTGTTTGA TTTCCATCTA
1981 ATCTTACAAA ATGACCATCA TGTTTTATTG CCATTCCAAA TCTGTCAGCA TCTGGGTCAT
2041 TCATAATAAT AATATCTGCA TCATGTTTAA TACCATATTC AAGCGGTATT TTTCATGCAG
2101 GATCAAATTC TGGATTTGGA TTTACAACAT TTTTAAATGT TTCATCTTCA AATGCATGCT
2161 CTTCAACCTC AATAACGTTA TATCCTGATT CACGTAATAT TTTTGGGGTA AATTTAGTTC
2221 CTGTTCCATT AACTGCGCTA AAAATAATTT TTAAATCTTT TTTAGCTTCT TGCTCTTTTT
2281 TGTACGTCTC TGTTTTAGAG CTAGAAATAG CAAGTTAAAA TAAGGCTAGT CCGTTATCAA
2341 CTTGAAAAAG TGGCACCGAG TCGGTGCTTT TTTAAGCTTG GCGTAACTAG ATCTTGAGAC
2401 AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG GATTGGGGGG TACAGTGCAG
2461 GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC TAAAGAATTA CAAAAACAAA
2521 TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG CAGAGATCCA CTTTGGCGCC
2581 GGCTCGAGGG GGCCCGGGTG CAAAGATGGA TAAAGTTTTA AACAGAGAGG AATCTTTGCA
2641 GCTAATGGAC CTTCTAGGTC TTGAAAGGAG TGGGAATTGG CTCCGGTGCC CGTCAGTGGG
2701 CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGATCCG
2761 GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC
2821 TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG AACGTTCTTT
2881 TTCGCAACGG GTTTGCCGCC AGAACACAGG TAAGTGCCGT GTGTGGTTCC CGCGGGCCTG
2941 GCCTCTTTAC GGGTTATGGC CCTTGCGTGC CTTGAATTAC TTCCACCTGG CTGCAGTACG
3001 TGATTCTTGA TCCCGAGCTT CGGGTTGGAA GTGGGTGGGA GAGTTCGAGG CCTTGCGCTT
3061 AAGGAGCCCC TTCGCCTCGT GCTTGAGTTG AGGCCTGGCC TGGGCGCTGG GGCCGCCGCG
3121 TGCGAATCTG GTGGCACCTT CGCGCCTGTC TCGCTGCTTT CGATAAGTCT CTAGCCATTT
3181 AAAATTTTTG ATGACCTGCT GCGACGCTTT TTTTCTGGCA AGATAGTCTT GTAAATGCGG
3241 GCCAAGATCT GCACACTGGT ATTTCGGTTT TTGGGGCCGC GGGCGGCGAC GGGGCCCGTG
3301 CGTCCCAGCG CACATGTTCG GCGAGGCGGG GCCTGCGAGC GCGGCCACCG AGAATCGGAC
3361 GGGGGTAGTC TCAAGCTGGC CGGCCTGCTC TGGTGCCTGG CCTCGCGCCG CCGTGTATCG
3421 CCCCGCCCTG GGCGGCAAGG CTGGCCCGGT CGGCACCAGT TGCGTGAGCG GAAAGATGGC
3481 CGCTTCCCGG CCCTGCTGCA GGGAGCTCAA AATGGAGGAC GCGGCGCTCG GGAGAGCGGG
3541 CGGGTGAGTC ACCCACACAA AGGAAAAGGG CCTTTCCGTC CTCAGCCGTC GCTTCATGTG
3601 ACTCCACGGA GTACCGGGCG CCGTCCAGGC ACCTCGATTA GTTCTCGAGC TTTTGGAGTA
3661 CGTCGTCTTT AGGTTGGGGG GAGGGGTTTT ATGCGATGGA GTTTCCCCAC ACTGAGTGGG
3721 TGGAGACTGA AGTTAGGCCA GCTTGGCACT TGATGTAATT CTCCTTGGAA TTTGCCCTTT
3781 TTGAGTTTGG ATCTTGGTTC ATTCTCAAGC CTCAGACAGT GGTTCAAAGT TTTTTTCTTC
3841 CATTTCAGGT GTCGTGACGT ACGGCCACCA TGACCGAGTA CAAGCCCACG GTGCGCCTCG
3901 CCACCCGCGA CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC
3961 CCGCCACGCG CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG
4021 AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG
4081 CCGCCGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA
4141 TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG
4201 GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGAGTCT
4261 CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG
4321 CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT
4381 ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT
4441 GGTGCATGAC CCGCAAGCCC GGTGCCTGAA CGCGTTAAGT CGACAATCAA CCTCTGGATT
4501 ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG
4561 GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT
4621 CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC
4681 AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA
4741 CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC
4801 TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT
4861 CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT
4921 GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC
4981 CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA
5041 CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCGTCGACT TTAAGACCAA TGACTTACAA
5101 GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA
5161 CTCCCAACGA AGACAAGATC TGCTTTTTGC TTGTACTGGG TCTCTCTGGT TAGACCAGAT
5221 CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT
5281 GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC
5341 CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT ACGTATAGTA GTTCATGTCA
5401 TCTTATTATT CAGTATTTAT AACTTGCAAA GAAATGAATA TCAGAGAGTG AGAGGAACTT
5461 GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA
5521 AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA
5581 TGTCTGGCTC TAGCTATCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT
5641 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC
5701 GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGGACG
5761 TACCCAATTC GCCCTATAGT GAGTCGTATT ACGCGCGCTC ACTGGCCGTC GTTTTACAAC
5821 GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT
5881 TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA
5941 GCCTGAATGG CGAATGGGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG
6001 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT
6061 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC
6121 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG
6181 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT
6241 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG
6301 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC
6361 TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA ATTTAGGTGG
6421 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA
6481 TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA
6541 GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT
6601 TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG
6661 TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG
6721 CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT
6781 ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA
6841 CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA
6901 ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC
6961 GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG
7021 CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC
7081 GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT
7141 AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT
7201 GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG
7261 GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT
7321 CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG
7381 TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT
7441 TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT
7501 CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA
7561 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA
7621 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC
7681 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA
7741 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT
7801 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG
7861 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG
7921 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC
7981 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG
8041 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT
8101 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG
8161 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA
8221 CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG
8281 AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC
8341 GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG
8401 CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG
8461 TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG
8521 TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA
8581 GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGAG CTGCAAGCTT AATGTAGTCT
8641 TATGCAATAC TCTTGTAGTC TTGCAACATG GTAACGATGA GTTAGCAACA TGCCTTACAA
8701 GGAGAGAAAA AGCACCGTGC ATGCCGATTG GTGGAAGTAA GGTGGTACGA TCGTGCCTTA
8761 TTAGGAAGGC AACAGACGGG TCTGACATGG ATTGGACGAA CCACTGAATT GCCGCATTGC
8821 AGAGATATTG TATTTAAGTG CCTAGCTCGA TACATAAACG GGTCTCTCTG GTTAGACCAG
8881 ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC
8941 TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA
9001 TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG AACAGGGACT
9061 TGAAAGCGAA AGGGAAACCA GAGGAGCTCT CTCGACGCAG GACTCGGCTT GCTGAAGCGC
9121 GCACGGCAAG AGGCGAGGGG CGGCGACTGG TGAGTACGCC AAAAATTTTG ACTAGCGGAG
9181 GCTAGAAGGA GAGAGATGGG TGCGAGAGCG TCAGTATTAA GCGGGGGAGA ATTAGATCGC
9241 GATGGGAAAA AATTCGGTTA AGGCCAGGGG GAAAGAAAAA ATATAAATTA AAACATATAG
9301 TATGGGCAAG CAGGGAGCTA GAACGATTCG CAGTTAATCC TGGCCTGTTA GAAACATCAG
9361 AAGGCTGTAG ACAAATACTG GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC
9421 TTAGATCATT ATATAATACA GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA
9481 AAGACACCAA GGAAGCTTTA GACAAGATAG AGGAAGAGCA AAACAAAAGT AAGACCACCG
9541 CACAGCAAGC GGCCGCTGAT CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA
9601 AGTGAATTAT ATAAATATAA AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAG
9661 GCAAAGAGAA GAGTGGTGCA GAGAGAAAAA AGAGCAGTGG GAATAGGAGC TTTGTTCCTT
9721 GGGTTCTTGG GAGCAGCAGG AAGCACTATG GGCGCAGCGT CAATGACGCT GACGGTACAG
9781 GCCAGACAAT TATTGTCTGG TATAGTGCAG CAGCAGAACA ATTTGCTGAG GGCTATTGAG
9841 GCGCAACAGC ATCTGTTGCA ACTCACAGTC TGGGGCATCA AGCAGCTCCA GGCAAGAATC
9901 CTGGCTGTGG AAAGATACCT AAAGGATCAA CAGCTCCTGG GGATTTGGGG TTGCTCTGGA
9961 AAACTCATTT GCACCACTGC TGTGCCTTGG AATGCTAGTT GGAGTAATAA ATCTCTGGAA
10021 CAGATTTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC
10081 TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA
10141 TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACA
//
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lentiGuide-Puro质粒载体应用举例
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