pYK421

LOCUS       pYK421           6978 bp DNA     circular SYN 11-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 27, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    232..819
                     /label=CMV
     primer_bind     863..882
                     /label=T7
     primer_bind     880..946
                     /label=oYF0019
     primer_bind     881..918
                     /label=oYK421_GNCR_F
     primer_bind     881..902
                     /label=oYK31-mCherry-NS3a-F
     primer_bind     881..899
                     /label=oYK06-ANR-mEGFP-F
     primer_bind     910..952
                     /label=oYF005
     CDS             919..1617
                     /codon_start=1
                     /label=GNCR1-N
                     /translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
                     AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
                     RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
                     AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
                     KAEEVKERCKS"
     primer_bind     919..952
                     /label=oYF0011
     primer_bind     919..943
                     /label=oLZ146 GNCR1-N F
     primer_bind     919..943
                     /label=oSCL762
     primer_bind     1585..1597
                     /label=oYK03-02-F
     primer_bind     1585..1596
                     /label=oYK31-mCherry-NS3a-F
     primer_bind     complement(1596..1653)
                     /label=oYF0020
     primer_bind     complement(1596..1647)
                     /label=oYF006
     primer_bind     complement(1596..1618)
                     /label=oYF0012
     primer_bind     complement(1605..1624)
                     /label=oYK465_GNCR1_R
     primer_bind     1614..1634
                     /label=oYK474_mCherry2_F
     primer_bind     1614..1632
                     /label=oYK474_mCherry_F
     primer_bind     complement(1617..1643)
                     /label=oDJ171-4
     primer_bind     1618..1695
                     /label=oDJ171-3
     primer_bind     complement(1619..1667)
                     /label=oLZ145 DNCR2-GNCR1 R
     primer_bind     1632..1652
                     /label=oLZ146 GNCR1-N F
     misc_feature    1634..1652
                     /label=linker
     primer_bind     1638..1690
                     /label=oYK03-02-F
     primer_bind     complement(1645..1668)
                     /label=oYK421_GNCR3_R
     primer_bind     1649..1686
                     /label=oYK421_mCherry_F
     misc_feature    1653
                     /label=linker
     misc_feature    1654..1668
                     /label=NSP3
     primer_bind     1669..1692
                     /label=oXQ135
     primer_bind     1669..1692
                     /label=oXQ174
     primer_bind     1669..1692
                     /label=omz_mCherry_F
     primer_bind     1669..1689
                     /label=oYK31-mCherry-NS3a-F
     CDS             1672..2376
                     /codon_start=1
                     /label=mCherry
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     1672..1698
                     /label=omz_linker_mCherry_F
     primer_bind     1713..1731
                     
     primer_bind     1713..1731
                     /label=DDL7AeF
                     /note="KpnI"
     primer_bind     1713..1731
                     /label=L7Ae FLP
     primer_bind     complement(1984..1995)
                     /label=oSCL011
     primer_bind     complement(1984..1995)
                     /label=omz_Coh2_R
     primer_bind     complement(1984..1995)
                     /label=omz_Coh2_His_R
     primer_bind     2117..2133
                     /label=mCherry_F测序
     primer_bind     2339..2393
                     /label=oYF005
     primer_bind     2348..2412
                     /label=oYK505_CAAX_F
     primer_bind     complement(2356..2475)
                     /label=oYK316_mCherry_CAAX_R
     primer_bind     complement(2356..2396)
                     /label=oDJ171-4
     primer_bind     complement(2356..2379)
                     /label=oXQ136
     primer_bind     complement(2356..2376)
                     /label=oXQ161
     primer_bind     complement(2356..2376)
                     /label=omz_mCherry_His_R
     primer_bind     complement(2356..2376)
                     /label=oYK56-mCherry-R
     primer_bind     complement(2356..2376)
                     /label=oYK58-mCherry-R
     primer_bind     complement(2358..2387)
                     /label=omz_mCherry_linker_R
     primer_bind     complement(2362..2382)
                     /label=oYK491_mCherry_R
     primer_bind     complement(2364..2382)
                     /label=oYK431_NES2_R
     primer_bind     complement(2369..2388)
                     /label=pYK472_mCherry_R
     primer_bind     complement(2373..2392)
                     /label=oYK275_mCherry_R
     primer_bind     2385..2401
                     /label=oLZ146 GNCR1-N F
     CDS             2395..2400
                     /codon_start=1
                     /label=4A/4B
                     /translation="DV"
     misc_feature    2413..2472
                     /label=CAAX
     primer_bind     2416..2451
                     /label=oYK491_CAAX_F
     primer_bind     complement(2432..2498)
                     /label=oYK421_CAAX_R
     primer_bind     complement(2473..2496)
                     /label=oYK431_NES2_R
     primer_bind     complement(2499..2516)
                     /label=oXQ161
     terminator      2572..2799
                     /label=bGH PA
     misc_feature    2845..3273
                     /label=f1 origin
     misc_feature    3278..3621
                     /label=SV40 early promoter and origin
     misc_feature    3467..3544
                     /label=SV40 ORI
     CDS             3686..4477
                     /label=Kan/neoR
     misc_feature    4651..4781
                     /label=SV40 early polyadenylation signal
     primer_bind     complement(4817..4837)
                     /label=M13-rev
     misc_binding    complement(4843..4865)
                     /label=LacO
     promoter        complement(4870..4899)
                     /label=lac
     misc_feature    complement(5164..5837)
                     /label=pUC origin
     CDS             complement(5985..6644)
                     /label=AmpR
     promoter        complement(6884..6912)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gctggctagg ccaccatgga catcgagaag ctgtgcaaga aggcggaaga ggaggccaag
      961 gaggcccagg agaaggccga cgagctgaga caacgacacc ccgattcgca ggcggcggaa
     1021 gacgcggagg atctcgcaaa tctggctgtc gccgccgtgc tgaccgcttg tctgctggcc
     1081 caggagcatc ccaacgccga tatcgctaag ctgtgcatca aggccgcgag cgaagctgcg
     1141 gaggccgcgt caaaagccgc cgagctggcc caacggcacc cagacagtca agcagccaga
     1201 gatgctatca aactcgcctc tcaagccgcc cgcgccgtga tcctggccat catgctcgcc
     1261 gcagagaatc ccaacgctga tattgcaaag ctatgtatta aggcggccag cgaagccgca
     1321 gaggctgcct cgaaagctgc ggaacttgca cagcggcacc ccgactccca ggccgcccga
     1381 gatgcaatta aactggccag tcaggccgct gaggcggtgg agagagccat ctggctggcc
     1441 gccgagaacc ccaatgccga tatcgccaaa aaatgcatca aagcggcctc cgaagcagcc
     1501 gaggaggcct ctaaagctgc cgaagaggct caaagacacc cagacagcca gaaggcacgc
     1561 gacgagatca aggaggcctc tcagaaggcc gaggaggtga aggagagatg caagagcgcc
     1621 agcggaagtg gcggaggagg cgacgtcgct agcatgctca agatggagat ggtgagcaag
     1681 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     1741 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1801 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     1861 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     1921 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     1981 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     2041 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     2101 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     2161 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     2221 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     2281 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     2341 cgccactcca ccggcggcat ggacgagctg tacaagggaa gcggcggagg aggcgacgtc
     2401 ggcagcggca gcaaaatgag taaggatgga aagaaaaaga aaaagaaaag caaaacaaag
     2461 tgtgtaataa tgtaaggatc cactagtcca gtgtggtgga attctgcaga tatccagcac
     2521 agtggcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     2581 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     2641 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     2701 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     2761 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     2821 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     2881 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     2941 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     3001 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     3061 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     3121 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     3181 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     3241 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     3301 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3361 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3421 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     3481 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3541 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3601 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
     3661 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     3721 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     3781 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     3841 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     3901 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     3961 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     4021 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     4081 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     4141 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     4201 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     4261 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     4321 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     4381 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     4441 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     4501 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     4561 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     4621 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     4681 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4741 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     4801 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     4861 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     4921 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     4981 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     5041 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5101 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5161 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5221 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     5281 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5341 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     5401 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     5461 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     5521 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     5581 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     5641 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     5701 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     5761 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     5821 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     5881 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     5941 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6001 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6061 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6121 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6181 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6241 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6301 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6361 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     6421 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     6481 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     6541 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     6601 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     6661 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     6721 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     6781 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     6841 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     6901 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     6961 aaagtgccac ctgacgtc
//