| 质粒名称: | pD649-HAsp-PTGFRN-Fc(DAPA)-AviTag-6xHis |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 156982 |
| 存储实验室: | Chris Garcia |
|
载体骨架基本信息 |
|
| 载体名称: | pD649 |
| 载体出品公司: | Clontech |
| 空载体大小: | 6106bp |
| 完整质粒大小: | 8527 |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | PTGFRN、FPRP |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 2421bp |
| 基因突变: | Fc region of human IgG contains D265A and P329A mutations (DAPA) |
| 启动子: | CMV/SP6 |
| 标签/融合蛋白: | Fc(DAPA)-AviTag-6xHis (C terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | GCGGCCGC (not destroyed) |
| 3’克隆位点: | GGCGCGCC (not destroyed) |
| 5’测序引物 | ATTTAGGTGACACTATAG |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pD649-HAsp-PTGFRN-Fc(DAPA)-AviTag-6xHis was a gift from Chris Garcia (Addgene plasmid # 156982 ; http://n2t.net/addgene:156982 ; RRID:Addgene_156982) |
| 参考文献部分: | A Human IgSF Cell-Surface Interactome Reveals a Complex Network of Protein-Protein Interactions. Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC. Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 10.1016/j.cell.2020.07.025. |
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pD649-HAsp-PTGFRN-Fc(DAPA)-AviTag-6xHis载体质粒基本信息
pD649-HAsp-PTGFRN-Fc(DAPA)-AviTag-6xHis质粒图谱载体图谱和pD649-HAsp-PTGFRN-Fc(DAPA)-AviTag-6xHis载体序列质粒序列多克隆位点信息
-AviTag-6xHis质粒图谱.png)
pD649-HAsp-PTGFRN-Fc(DAPA)-AviTag-6xHis质粒载体简介
pD649-HAsp-PTGFRN-Fc(DAPA)-AviTag-6xHis质粒序列载体序列
LOCUS pD649-HAsp-PTGFRN-Fc(DAPA)-AviTag-6xHis 8527 bp DNA circular SYN 15-DEC-2025
DEFINITION Mammalian expression of cell-surface protein extracellular domain
fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8527)
AUTHORS Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL,
Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser
SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
TITLE A Human IgSF Cell-Surface Interactome Reveals a Complex Network of
Protein-Protein Interactions.
JOURNAL Cell. 2020 Aug 20;182(4):1027-1043.e17. doi:
10.1016/j.cell.2020.07.025.
PUBMED 32822567
REFERENCE 2 (bases 1 to 8527)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20-
20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT Sequence Label: pD649-HAsp-PTGFRN-Fc(DAPA)-AviTag-6xHis
FEATURES Location/Qualifiers
source 1..8527
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 124..143
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 149..168
/label=pENTR-R
/note="pENTR vectors, reverse primer"
rep_origin 254..842
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 743..762
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 996..1013
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 1111..1128
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 1112..1128
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 1584..1600
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
enhancer 1767..2146
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2147..2350
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2301..2321
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 2347..2371
/label=LNCX
/note="Human CMV promoter, forward primer"
promoter 2581..2599
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 2581..2598
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind 2612..2631
/label=pMT2-F
/note="Synthetic intron, forward primer"
CDS 5869..5913
/codon_start=1
/product="peptide tag that allows for enzymatic
biotinylation"
/label=AviTag(TM)
/translation="GLNDIFEAQKIEWHE"
CDS 5914..5931
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(6071..6088)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6077..6188
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(6306..6326)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6311..6640
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6491..6626
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6553..6572
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 6688..6735
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 6754..7353
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(6754..6773)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 7250..7270
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
polyA_signal 7483..7604
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7520..7539)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7574..7593
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
CDS complement(join(7657..8448,8449..8517))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8280..8299
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(join(8518..8527,1..82))
/gene="bla"
/label=AmpR promoter
ORIGIN
1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccgt tcctacagcc
2761 acacttgtta gagtcgtggg aaccgagctg gtcatcccct gcaacgtgtc cgattacgat
2821 ggccccagcg agcagaactt cgactggtct tttagcagcc tgggcagcag ctttgtggaa
2881 ctggcctcta cctgggaagt gggctttccc gctcagctgt accaagagag actgcagaga
2941 ggcgagatcc tgctgagaag aaccgccaac gatgccgtgg aactgcacat caagaacgtg
3001 cagcctagcg accagggcca ctacaagtgt agcacaccta gcaccgatgc caccgtgcag
3061 ggcaattacg aggacaccgt gcaagtgaag gtgctggccg attctctgca cgtgggacca
3121 tctgctagac ctccacctag cctgtctctg agagagggcg agcctttcga gctgagatgt
3181 acagccgctt ctgcctctcc tctgcacaca catctggctc tgctgtggga agtgcataga
3241 ggcccagcta gaagatccgt gctggccctg acacacgagg gcagatttca ccctggactg
3301 ggctacgagc agagatacca tagcggagat gtgcggctgg ataccgtggg ctctgatgcc
3361 tacagactgt ctgtgtcaag agccctgagc gccgatcagg gcagctacag atgtatcgtg
3421 tctgagtgga tcgccgagca aggcaactgg caagagatcc aagagaaggc cgtggaagtg
3481 gccacagtgg tcattcagcc atctgtgctg agagccgccg tgcctaaaaa tgtgtctgtg
3541 gccgagggca aagagctgga cctgacctgc aacatcacca ccgacagagc cgatgatgtc
3601 cggcctgaag tgacctggtc cttctccaga atgcccgata gcacactgcc tggaagcaga
3661 gtgctggcca gactggatag agatagcctg gtgcactcta gccctcacgt ggccctgtct
3721 catgtggacg ccagatccta tcatctgctt gtgcgggacg tgtccaaaga gaacagcggc
3781 tactactact gccacgtgtc actgtgggcc cctggccaca atagatcttg gcacaaagtg
3841 gccgaagccg tgtcatctcc tgctggcgtt ggagtgacat ggctggaacc cgattaccag
3901 gtgtacctga atgccagcaa ggtgcccggc tttgccgatg atcctacaga gctggcctgc
3961 agagtggtgg atacaaagtc tggcgaggcc aacgtgcggt tcaccgtgtc ctggtactac
4021 agaatgaacc ggcggagcga caacgtggtc acatctgaac tgctggccgt gatggatggc
4081 gactggacac tgaagtacgg cgagagatct aagcagagag cccaggacgg cgacttcatc
4141 ttcagcaaag agcacaccga caccttcaac ttcagaatcc agcggaccac cgaagaggac
4201 cggggcaatt attactgtgt ggtgtccgcc tggaccaagc agagaaacaa cagctgggtc
4261 aagagcaagg acgtgttcag caagcccgtg aacatcttct gggccctcga ggattctgtg
4321 ctggtggtta aggccagaca gcccaagcct ttctttgccg ccggaaacac cttcgagatg
4381 acatgcaagg tgtccagcaa gaacatcaag tccccacggt acagcgtgct gatcatggcc
4441 gagaaacctg tgggcgacct gagcagcccc aacgagacaa agtacatcat ctccctggac
4501 caggacagcg tggtcaagct ggaaaactgg accgacgctt ccagagtgga tggcgtggtg
4561 ctggaaaagg tgcaagagga cgagttccgg tacaggatgt accagacaca ggtgtccgat
4621 gccggcctgt acagatgcat ggttacagct tggagccctg tgcggggaag cctttggaga
4681 gaagctgcca caagcctgag caaccctatc gagatcgact tccagacaag cggccccatc
4741 ttcaatgcca gcgtgcactc tgatacccct agcgtgatcc ggggcgatct gatcaagctg
4801 ttctgcatca tcaccgtgga aggcgccgct ctggaccctg atgatatggc cttcgatgtg
4861 tcttggtttg ccgtgcacag cttcggcctg gataaggctc ctgttctgct gtcctctctg
4921 gacagaaagg gcatcgtgac cacctccaga agagactgga agtctgacct gagcctggaa
4981 agggtgtccg tgctcgagtt tctgctgcaa gtgcacggct ccgaggacca ggatttcggc
5041 aactactatt gcagcgtgac cccttgggtc aagtctccta caggcagctg gcagaaagag
5101 gccgagattc acagcaagcc tgtgtttatc accgtgaaga tggacgtgct gaacgccttc
5161 aagtaccctg gcgcgccggg cggcgacaag acccacacct gccccccctg cccagctcca
5221 gaactgctgg gagggccttc cgtgttcctg ttccccccca agcccaagga caccctgatg
5281 atcagcagga cccccgaggt gacctgcgtg gtggtggccg tgtcccacga ggacccagag
5341 gtgaagttca actggtacgt ggacggcgtg gaggtgcaca acgccaagac caagcccaga
5401 gaggagcagt acaacagcac ctacagggtg gtgtccgtgc tgaccgtgct gcaccaggac
5461 tggctgaacg gcaaagaata caagtgcaaa gtctccaaca aggccctggc tgccccaatc
5521 gaaaagacaa tcagcaaggc caagggccag ccacgggagc cccaggtgta caccctgccc
5581 cccagccggg aggagatgac caagaaccag gtgtccctga cctgtctggt gaagggcttc
5641 taccccagcg atatcgccgt ggagtgggag agcaacggcc agcccgagaa caactacaag
5701 accacccccc cagtgctgga cagcgacggc agcttcttcc tgtacagcaa gctgaccgtg
5761 gacaagtcca ggtggcagca gggcaacgtg ttcagctgca gcgtgatgca cgaggccctg
5821 cacaaccact acacccagaa gtccctgagc ctgagccccg gcggcagcgg tcttaatgat
5881 attttcgagg ctcagaagat tgagtggcat gaacaccacc accatcacca ctaaggccgg
5941 cctgtagtca ctggcacaac gcgttacccg gtaagccaat cgggtataca cggtcgtcat
6001 actgcagaca gggttcttct actttgcaag atagtcttga gtagtaaaat aaatagatag
6061 agaaaatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
6121 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
6181 attgcatcac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
6241 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
6301 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
6361 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
6421 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
6481 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
6541 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
6601 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
6661 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
6721 atagtataat acgacaaggt gaggaactaa accatgaccg agtacaagcc cacggtgcgc
6781 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
6841 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
6901 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
6961 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
7021 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
7081 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
7141 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
7201 gcggccgagc gcgccggggt gcccgccttc ctggagacat ccgcgccccg caacctcccc
7261 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
7321 acctggtgca tgacccgcaa gcccggtgcc tgacacgtgc tacgagattt cgattccacc
7381 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
7441 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
7501 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
7561 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
7621 tcgacctcta gctagagctt ggcgtaatca tggtcattac caatgcttaa tcagtgaggc
7681 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
7741 gataactacg atacgggagg gcttaccatc tggccccagc gctgcgatga taccgcgaga
7801 accacgctca ccggctccgg atttatcagc aataaaccag ccagccggaa gggccgagcg
7861 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
7921 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccatcg ctacaggcat
7981 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
8041 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
8101 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
8161 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
8221 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
8281 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
8341 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
8401 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
8461 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatatt
8521 cttcctt
//
