| 质粒名称: | pLVPT-tTR-KRAB |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 11642 |
| 存储实验室: | Patrick Aebischer, Didier Trono |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 11640 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mPGK, GFP, tTR-KRAB, Tet-on |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | See map |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLVPT-tTR-KRAB was a gift from Patrick Aebischer & Didier Trono (Addgene plasmid # 11642 ; http://n2t.net/addgene:11642 ; RRID:Addgene_11642) |
| 参考文献部分: | A versatile tool for conditional gene expression and knockdown. Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P. Nat Methods. 2006 Feb . 3(2):109-16. 10.1038/nmeth846 PubMed 16432520 |
买家导航
pLVPT-tTR-KRAB载体质粒基本信息
pLVPT-tTR-KRAB质粒图谱载体图谱和pLVPT-tTR-KRAB载体序列质粒序列多克隆位点信息
pLVPT-tTR-KRAB质粒载体简介
pLVPT-tTR-KRAB质粒序列载体序列
LOCUS pLVPT-tTR-KRAB 11634 bp ds-DNA circular SYN 29-AUG-2019
DEFINITION Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK
promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM
(Addgene#12247)) - 2nd generation.
ACCESSION .
VERSION .
KEYWORDS pLVPT-tTR-KRAB
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11634)
AUTHORS Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
TITLE A versatile tool for conditional gene expression and knockdown.
JOURNAL Nat Methods. 2006 Feb . 3(2):109-16.
PUBMED 16432520
REFERENCE 2 (bases 1 to 11634)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 29, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11634
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 294..411
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 450..949
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(485..508)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 882..901
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
regulatory 1000..1009
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1006..1722
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(1051..1072)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1312..1331)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1659..1680
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 1818..2369
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(1962..1979)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 2189..2208
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 2372..2989
/codon_start=1
/gene="tetR from transposon Tn10"
/product="tetracycline repressor TetR"
/label=TetR
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG"
primer_bind complement(2445..2464)
/label=Tet-R
/note="Tetracycline resistance gene, reverse primer"
CDS 2993..3013
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 3116..3310
/codon_start=1
/gene="ZNF10"
/gene_synonym="KOX1"
/product="Kruppel-associated box (KRAB) transcriptional
repression domain from the human zinc finger protein ZNF10
(Margolin et al., 1994)"
/label=KRAB
/translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
QLTKPDVILRLEKGEEPWLV"
primer_bind complement(3449..3465)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3450..3466)
/label=pBluescriptKS
/note="For pBluescript vector"
misc_feature 3480..4068
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3533..3553)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3951..3962)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
protein_bind 4206..4476
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
LTR 4515..4695
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
promoter complement(4720..4738)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(4721..4738)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(5028..5047)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(5261..5280)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 5380..5402
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(5440..5458)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 5526..5630
/gene="bla"
/label=AmpR promoter
CDS 5631..6491
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5849..5868)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6662..7250
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7151..7170
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 7404..7421
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(7491..7511)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7496..7825
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7676..7811
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7738..7757
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 8959..9024
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 9154..9174
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 9599..9733
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9636..9655)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9690..9709
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 9901..10534
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 10581..10706
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 11199..11432
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
ORIGIN
1 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
61 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
121 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
181 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
241 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa
301 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
361 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac
421 gagactagcc tcgacgatgg tcgagtaccg ggtaggggag gcgcttttcc caaggcagtc
481 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
541 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
601 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
661 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
721 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
781 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
841 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa
901 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctctagcgg
961 gatccaggcc taagcttacg cgtcctagcg ctaccggtcg ccaccatggt gagcaagggc
1021 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
1081 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
1141 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
1201 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
1261 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
1321 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
1381 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
1441 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
1501 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
1561 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
1621 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
1681 accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact cagatctcga
1741 ctagctagta gctagctagc tagtcgagct caagcttcga attcccgggc tgcaggaatt
1801 ccgccccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
1861 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
1921 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
1981 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
2041 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
2101 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
2161 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
2221 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
2281 ctttacatgt gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg
2341 gttttccttt gaaaaacacg atgataatac catggctaga ttagataaaa gtaaagtgat
2401 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
2461 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc
2521 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
2581 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt
2641 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta
2701 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc
2761 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga
2821 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt
2881 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct
2941 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt cgccaaaaaa
3001 gaagagaaag gtcgacggcg gtggtgcttt gtctcctcag cactctgctg tcactcaagg
3061 aagtatcatc aagaacaagg agggcatgga tgctaagtca ctaactgcct ggtcccggac
3121 actggtgacc ttcaaggatg tatttgtgga cttcaccagg gaggagtgga agctgctgga
3181 cactgctcag cagatcgtgt acagaaatgt gatgctggag aactataaga acctggtttc
3241 cttgggttat cagcttacta agccagatgt gatcctccgg ttggagaagg gagaagagcc
3301 ctggctggtg gagagagaaa ttcaccaaga gacccatcct gattcagaga ctgcatttga
3361 aatcaaatca tcagtttaag gatctcgact agctagtagc tagctagcta gtcgagctca
3421 agcttcgaat tcgatatcaa gcttatcgcg ataccgtcga cctcgactag tcatatgata
3481 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
3541 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
3601 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
3661 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
3721 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
3781 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
3841 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
3901 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
3961 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
4021 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cggtacgtat
4081 ggccaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
4141 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctcgagttt
4201 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
4261 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
4321 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
4381 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
4441 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcgg atcgatctgc
4501 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
4561 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
4621 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
4681 ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg
4741 tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta
4801 caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac
4861 tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca
4921 cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg
4981 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
5041 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
5101 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
5161 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
5221 ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg
5281 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
5341 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
5401 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
5461 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
5521 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
5581 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
5641 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
5701 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
5761 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
5821 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
5881 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
5941 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
6001 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
6061 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
6121 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
6181 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
6241 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
6301 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
6361 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
6421 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
6481 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
6541 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
6601 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
6661 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
6721 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
6781 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
6841 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
6901 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
6961 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
7021 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
7081 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
7141 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
7201 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
7261 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
7321 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
7381 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
7441 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg
7501 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
7561 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
7621 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
7681 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
7741 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
7801 ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt
7861 tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat
7921 gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct
7981 cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca
8041 tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt
8101 ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact
8161 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
8221 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
8281 tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa
8341 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
8401 tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt
8461 cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt
8521 taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
8581 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
8641 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa
8701 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc
8761 cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg
8821 atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc
8881 cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
8941 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
9001 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
9061 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
9121 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
9181 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
9241 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
9301 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
9361 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
9421 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
9481 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
9541 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
9601 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
9661 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
9721 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
9781 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
9841 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
9901 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
9961 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
10021 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
10081 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
10141 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
10201 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
10261 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
10321 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
10381 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
10441 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
10501 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
10561 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
10621 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
10681 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
10741 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
10801 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
10861 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
10921 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
10981 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
11041 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
11101 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
11161 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
11221 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
11281 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
11341 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
11401 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
11461 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
11521 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
11581 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga atta
//
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pLVPT-tTR-KRAB质粒载体应用举例
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