| 质粒名称: | pMH455 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002797 |
| 产品用途: | Mammalian PhCMV-driven constitutive NanoLuc expression vector (PhCMV-NanoLuc-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | NanoLuc |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
pMH455
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pMH455载体质粒基本信息
pMH455质粒图谱载体图谱和pMH455载体序列质粒序列多克隆位点信息
pMH455质粒载体简介
pMH455质粒序列载体序列
LOCUS pMH455 6020 bp DNA circular SYN 15-JUL-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6020)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6020
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 235..818
/label=P_CMV
/label=nonstandard type: promoter_const
misc_feature 235..614
/label=P_CMV_enhancer
/label=nonstandard type: promoter_element
primer_bind 458..488
/label=oMH809
misc_feature 615..818
/label=P_CMV_IE
/label=nonstandard type: promoter_element
primer_bind 769..789
/label=oMHS-CMV-F
misc_feature 829
/label=putative transcriptional start
promoter 863..882
/label=T7 promoter
primer_bind 895..958
/label=oMH1362
primer_bind 915..934
/label=pMH1649
primer_bind 918..956
/label=oMH469
primer_bind 929..958
/label=oMH1594
primer_bind complement(935..987)
/label=oMH1419
sig_peptide 941..1009
/codon_start=1
/label=Secretory (IgK)
/label=nonstandard type: signal
/translation="MTSETDTLLLWVLLLWVPGSTGD"
primer_bind 941..967
/label=oMH1291
primer_bind 943..968
/label=oMH1293
primer_bind 943..967
/label=oMH1354
primer_bind 944..968
/label=oMH1292
primer_bind 966..1014
/label=oMH1420
primer_bind complement(993..1015)
/label=oMH1421
primer_bind complement(1007..1033)
/label=oMH1350
primer_bind complement(1007..1028)
/label=oMH1355
primer_bind complement(1007..1027)
/label=oMH1285
primer_bind complement(1007..1027)
/label=oMH1345
primer_bind complement(1007..1027)
/label=oMH1351
primer_bind 1013..1037
/label=oMH1288
primer_bind 1028..1056
/label=oMH563
primer_bind 1028..1056
/label=pMH1649
CDS 1031..1540
/codon_start=1
/label=NLuc (nano Luciferase)
/label=nonstandard type: reporter
/translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
LCERILA"
primer_bind 1228..1245
/label=oMHS-nLuc
primer_bind complement(1522..1563)
/label=oMH941
primer_bind complement(1522..1540)
/label=oMH1294
primer_bind complement(1522..1540)
/label=oMH1456
primer_bind complement(1526..1550)
/label=oMH1595
primer_bind complement(1526..1540)
/label=oMH564
primer_bind complement(1529..1540)
/label=oMH330
terminator 1617..1844
/label=bGH polyA
primer_bind complement(1667..1688)
/label=oMHS-bGH
rep_origin 1890..2318
/label=f1 bacteriophage origin of replication
/label=f1 bacteriophage origin of replication; arrow _f1
ori
misc_feature 2332..2661
/label=P_SV40
/label=nonstandard type: promoter_const
rep_origin 2512..2647
/label=SV40 ORI
misc_feature 2564..2613
/label=P_SV40 TSS-region
CDS 2728..3522
/label=Translation 2136-2930
misc_feature 2728..3519
/label=Kan/neoR
/label=nonstandard type: resistance
primer_bind complement(3862..3882)
/label=M13-rev
misc_binding complement(3888..3910)
/label=LacO
promoter complement(3914..3944)
/label=lac promoter
rep_origin complement(4268..4853)
/direction=LEFT
/label=high-copy-number ColE1/pMB1/pBR322/pUC origin o
/label=high-copy-number ColE1/pMB1/pBR322/pUC origin of
_ori
CDS complement(5024..5884)
/label=Translation 5292-4432
misc_feature complement(5027..5884)
/label=AmpR
/label=nonstandard type: resistance
promoter complement(5885..5989)
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atgactagtg agacagacac
961 actcctgcta tgggtactgc tgctctgggt tccaggttcc actggtgacg ctagtggtgg
1021 ttctggtatg gtcttcacac tcgaagattt cgttggggac tggcgacaga cagccggcta
1081 caacctggac caagtccttg aacagggagg tgtgtccagt ttgtttcaga atctcggggt
1141 gtccgtaact ccgatccaaa ggattgtcct gagcggtgaa aatgggctga agatcgacat
1201 ccatgtcatc atcccgtatg aaggtctgag cggcgaccaa atgggccaga tcgaaaaaat
1261 ttttaaggtg gtgtaccctg tggatgatca tcactttaag gtgatcctgc actatggcac
1321 actggtaatc gacggggtta cgccgaacat gatcgactat ttcggacggc cgtatgaagg
1381 catcgccgtg ttcgacggca aaaagatcac tgtaacaggg accctgtgga acggcaacaa
1441 aattatcgac gagcgcctga tcaaccccga cggctccctg ctgttccgag taaccatcaa
1501 cggagtgacc ggctggcggc tgtgcgaacg cattctggcg taagaattct gcagatatcc
1561 agcacagtgg cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac
1621 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
1681 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
1741 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
1801 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
1861 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
1921 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
1981 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
2041 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
2101 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
2161 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
2221 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
2281 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
2341 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
2401 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
2461 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
2521 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
2581 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
2641 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
2701 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
2761 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
2821 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
2881 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
2941 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
3001 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
3061 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
3121 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
3181 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
3241 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
3301 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
3361 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
3421 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
3481 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
3541 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
3601 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
3661 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
3721 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
3781 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
3841 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
3901 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
3961 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
4021 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
4081 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
4141 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
4201 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
4261 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
4321 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
4381 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
4441 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
4501 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
4561 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
4621 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
4681 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
4741 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
4801 gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
4861 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
4921 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
4981 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
5041 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
5101 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
5161 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
5221 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
5281 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
5341 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
5401 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
5461 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
5521 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
5581 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
5641 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
5701 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
5761 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
5821 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
5881 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
5941 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
6001 gaaaagtgcc acctgacgtc
//
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