pTCK303-bk1-3Flag-bk2

LOCUS       pTCK303-bk1-3Flag-bk2            15446 bp DNA     circular SYN 25-MAR-2026
DEFINITION  pTCK303.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..15446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(23..368)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     745..761
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        777..2768
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     misc_feature    2685..2702
                     /label=303F
     CDS             2817..2888
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDKM"
     misc_feature    2895..2915
                     /label=同源臂
     misc_feature    2928..2948
                     /label=同源臂
     CDS             2949..2958
                     /codon_start=1
                     /label=Feature 22
                     /translation="EFP"
     terminator      2959..3211
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3008..3027
                     /label=NOSR
     primer_bind     complement(3228..3244)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3252..3268
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3276..3306)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3321..3342
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3533..4209
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4276..5301
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5342..5516
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5594..5618
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6043..6837
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      8123..8711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    8897..9037
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(11143..11772)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13073..13097
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      13119..13371
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(13406..13423)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(13430..15247,15438..15446,1..6))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          15248..15437
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 taccatggtc aagagtcccc cgtgttctct ccaaatgaaa tgaacttcct tatatagagg
       61 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
      121 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
      181 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttcaacg atggcctttc
      241 ctttatcgca atgatggcat ttgtaggagc caccttcctt ttccactatc ttcacaataa
      301 agtgacagat agctgggcaa tggaatccga ggaggtttcc ggatattacc ctttgttgaa
      361 aagtctcaat tgccctttgg tcttctgaga ctgtatcttt gatatttttg gagtagacaa
      421 gtgtgtcgtg ctccaccatg ttgacgaaga ttttcttctt gtcattgagt cgtaagagac
      481 tctgtatgaa ctgttcgcca gtctttacgg cgagttctgt taggtcctct atttgaatct
      541 ttgactccat gaagctaaac tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc
      601 tctagcattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
      661 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
      721 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc
      781 agtgcagcgt gacccggtcg tgcccctctc tagagataat gagcattgca tgtctaagtt
      841 ataaaaaatt accacatatt ttttttgtca cacttgtttg aagtgcagtt tatctatctt
      901 tatacatata tttaaacttt actctacgaa taatataatc tatagtacta caataatatc
      961 agtgttttag agaatcatat aaatgaacag ttagacatgg tctaaaggac aattgagtat
     1021 tttgacaaca ggactctaca gttttatctt tttagtgtgc atgtgttctc cttttttttt
     1081 gcaaatagct tcacctatat aatacttcat ccattttatt agtacatcca tttagggttt
     1141 agggttaatg gtttttatag actaattttt ttagtacatc tattttattc tattttagcc
     1201 tctaaattaa gaaaactaaa actctatttt agttttttta tttaataatt tagatataaa
     1261 atagaataaa ataaagtgac taaaaattaa acaaataccc tttaagaaat taaaaaaact
     1321 aaggaaacat ttttcttgtt tcgagtagat aatgccagcc tgttaaacgc cgtcgacgag
     1381 tctaacggac accaaccagc gaaccagcag cgtcgcgtcg ggccaagcga agcagacggc
     1441 acggcatctc tgtcgctgcc tctggacccc tctcgagagt tccgctccac cgttggactt
     1501 gctccgctgt cggcatccag aaattgcgtg gcggagcggc agacgtgagc cggcacggca
     1561 ggcggcctcc tcctcctctc acggcaccgg cagctacggg ggattccttt cccaccgctc
     1621 cttcgctttc ccttcctcgc ccgccgtaat aaatagacac cccctccaca ccctctttcc
     1681 ccaacctcgt gttgttcgga gcgcacacac acacaaccag atctccccca aatccacccg
     1741 tcggcacctc cgcttcaagg tacgccgctc gtcctccccc ccccccctct ctaccttctc
     1801 tagatcggcg ttccggtcca tggttagggc ccggtagttc tacttctgtt catgtttgtg
     1861 ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat gcgacctgta
     1921 cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat cctgggatgg
     1981 ctctagccgt tccgcagacg ggatcgattt catgattttt tttgtttcgt tgcatagggt
     2041 ttggtttgcc cttttccttt atttcaatat atgccgtgca cttgtttgtc gggtcatctt
     2101 ttcatgcttt tttttgtctt ggttgtgatg atgtggtctg gttgggcggt cgttctagat
     2161 cggagtagaa ttctgtttca aactacctgg tggatttatt aattttggat ctgtatgtgt
     2221 gtgccataca tattcatagt tacgaattga agatgatgga tggaaatatc gatctaggat
     2281 aggtatacat gttgatgcgg gttttactga tgcatataca gagatgcttt ttgttcgctt
     2341 ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg ttctagatcg gagtagaata
     2401 ctgtttcaaa ctacctggtg tatttattaa ttttggaact gtatgtgtgt gtcatacatc
     2461 ttcatagtta cgagtttaag atggatggaa atatcgatct aggataggta tacatgttga
     2521 tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca tatgctctaa
     2581 ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt gatcttgata
     2641 tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct gccttcatac
     2701 gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt ttggtgttac
     2761 ttctgcaggt cgactctcga ggatccggta cccggtatcg atttaaaagg cctatggact
     2821 acaaggatga cgacgacaag gactacaagg atgacgacga caaggactac aaggacgacg
     2881 acaagatgag gcctcaggtc gactctcgag gatcaactag tgagctcagt acccggtatc
     2941 gatttaaaga atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat
     3001 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta
     3061 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
     3121 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
     3181 tcgcgcgcgg tgtcatctat gttactagat cgggaattcg taatcatgtc atagctgttt
     3241 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     3301 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     3361 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     3421 gggagaggcg gtttgcgtat tggctagagc agcttgccaa catggtggag cacgacactc
     3481 tcgtctactc caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt
     3541 ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact
     3601 tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag
     3661 gaaaggctat cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca
     3721 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
     3781 gtgaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca
     3841 gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga
     3901 ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc
     3961 tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc tgccgacagt
     4021 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
     4081 acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa
     4141 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
     4201 acacgctgaa atcaccagtc tctctctaca aatctatctc tctcgagctt tcgcagatcc
     4261 ggggggcaat gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     4321 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     4381 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     4441 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     4501 gaagtgcttg acattgggga gtttagcgag agcctgacct attgcatctc ccgccgttca
     4561 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct acaaccggtc
     4621 gcggaggcta tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     4681 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     4741 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     4801 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     4861 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     4921 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     4981 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     5041 cttgcaggat cgccacgact ccgggcgtat atgctccgca ttggtcttga ccaactctat
     5101 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     5161 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     5221 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     5281 cgtccgaggg caaagaaata gagtagatgc cgaccgggat ctgtcgatcg acaagctcga
     5341 gtttctccat aataatgtgt gagtagttcc cagataaggg aattagggtt cctatagggt
     5401 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
     5461 tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaaaatc cagatccccc
     5521 gaattaattc ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc
     5581 taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg
     5641 accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg
     5701 tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa
     5761 gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg
     5821 attgtaacga tgacagagcg ttgctgcctg tgatcaccgc ggtttcaaaa tcggctccgt
     5881 cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt
     5941 aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg
     6001 gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata
     6061 tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg
     6121 tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg
     6181 gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg
     6241 ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc
     6301 aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa
     6361 agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac
     6421 atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta
     6481 ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa
     6541 gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt
     6601 tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt
     6661 attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg
     6721 tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg
     6781 atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac
     6841 ctagggaagg tgcgaataag cggggaaatt cttctcggct gactcagtca tttcatttct
     6901 tcatgtttga gccgattttt tctcccgtaa atgccttgaa tcagcctatt tagaccgttt
     6961 cttcgccatt taaggcgtta tccccagttt ttagtgagat ctctcccact gacgtatcat
     7021 ttggtccgcc cgaaacaggt tggccagcgt gaataacatc gccagttggt tatcgttttt
     7081 cagcaacccc ttgtatctgg ctttcacgaa gccgaactgt cgcttgatga tgcgaaatgg
     7141 gtgctccacc ctggcccgga tgctggcttt catgtattcg atgttgatgg ccgttttgtt
     7201 cttgcgtgga tgctgtttca aggttcttac cttgccgggg cgctcggcga tcagccagtc
     7261 cacatccacc tcggccagct cctcgcgctg tggcgcccct tggtagccgg catcggctga
     7321 gacaaattgc tcctctccat gcagcagatt acccagctga ttgaggtcat gctcgttggc
     7381 cgcggtggtg accaggctgt gggtcaggcc actcttggca tcgacaccaa tgtgggcctt
     7441 catgccaaag tgccactgat tgcctttctt ggtctgatgc atctccggat cgcgttgctg
     7501 ctctttgttc ttggtcgagc tgggtgcctc aatgatggtg gcatcgacca aggtgccttg
     7561 agtcatcatg acgcctgctt cggccagcca gcgattgatg gtcttgaaca attggcgggc
     7621 cagttgatgc tgctccagca ggtggcggaa attcatgatg gtggtgcggt ccggcaaggc
     7681 gctatccagg gataaccggg caaacagacg catggaggcg atttcgtaca gagcatcttc
     7741 catcgcgcca tcgctcaggt tgtaccaatg ctgcatgcag tgaatgcgta gcatggtttc
     7801 cagcggataa ggtcgccggc cattaccagc cttggggtaa aacggctcga tgacttccac
     7861 catgttttgc catggcagaa tctgctccat gcgggacaag aaaatctctt ttctggtctg
     7921 acggcgctta ctgctgaatt cactgtcggc gaaggtaagt tgatgactca tgatgaaccc
     7981 tgttctatgg ctccagatga caaacatgat ctcatatcag ggacttgttc gcaccttccc
     8041 tagaatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8101 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8161 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8221 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     8281 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8341 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8401 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8461 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8521 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8581 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8641 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8701 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8761 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8821 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8881 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     8941 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     9001 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     9061 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     9121 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
     9181 tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta
     9241 gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg
     9301 aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg
     9361 ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt
     9421 ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt
     9481 gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt
     9541 acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt
     9601 cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt
     9661 cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct
     9721 ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga
     9781 acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg
     9841 cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat
     9901 cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct
     9961 cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct
    10021 ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt
    10081 tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt
    10141 ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg
    10201 caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca
    10261 tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca
    10321 tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct
    10381 cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac
    10441 tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct
    10501 tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga
    10561 ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc
    10621 gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga
    10681 tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc
    10741 agtgccattg cagggccggc aggcaaccca gccgcttacg cctggccaac cgcccgttcc
    10801 tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc
    10861 tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct
    10921 gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct
    10981 tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt
    11041 ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta
    11101 gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt
    11161 aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag
    11221 aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc
    11281 aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc
    11341 tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg
    11401 ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac
    11461 cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg
    11521 cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt
    11581 cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg
    11641 ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc
    11701 gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc
    11761 gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca
    11821 gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc
    11881 tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg
    11941 gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg
    12001 gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc
    12061 tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg
    12121 cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt
    12181 cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg
    12241 cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg
    12301 gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc
    12361 tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca
    12421 acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc
    12481 ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca
    12541 ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg
    12601 ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc
    12661 cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg
    12721 catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt
    12781 cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca
    12841 gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct
    12901 gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt
    12961 gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt
    13021 ttcacgccct tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc
    13081 gccaatatat cctgtcaaac actgatagtt taattcccga tctagtaaca tagatgacac
    13141 cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat
    13201 gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat
    13261 gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc
    13321 ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat cggggaaatt
    13381 cgagctggtc acctgtaatt cacacgtggt ggtggtggtg gtggctagct tgtttgcctc
    13441 cctgctgcgg tttttcaccg aagttcatgc cagtccagcg tttttgcagc agaaaagccg
    13501 ccgacttcgg tttgcggtcg cgagtgaaga tccctttctt gttaccgcca acgcgcaata
    13561 tgccttgcga ggtcgcaaaa tcggcgaaat tccatacctg ttcaccgacg acggcgctga
    13621 cgcgatcaaa gacgcggtga tacatatcca gccatgcaca ctgatactct tcactccaca
    13681 tgtcggtgta cattgagtgc agcccggcta acgtatccac gccgtattcg gtgatgataa
    13741 tcggctgatg cagtttctcc tgccaggcca gaagttcttt ttccagtacc ttctctgccg
    13801 tttccaaatc gccgctttgg acataccatc cgtaataacg gttcaggcac agcacatcaa
    13861 agagatcgct gatggtatcg gtgtgagcgt cgcagaacat tacattgacg caggtgatcg
    13921 gacgcgtcgg gtcgagttta cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac
    13981 cttgcggacg ggtatccggt tcgttggcaa tactccacat caccacgctt gggtggtttt
    14041 tgtcacgcgc tatcagctct ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga
    14101 ctgcctcttc gctgtacagt tctttcggct tgttgcccgc ttcgaaacca atgcctaaag
    14161 agaggttaaa gccgacagca gcagtttcat caatcaccac gatgccatgt tcatctgccc
    14221 agtcgagcat ctcttcagcg taagggtaat gcgaggtacg gtaggagttg gccccaatcc
    14281 agtccattaa tgcgtggtcg tgcaccatca gcacgttatc gaatcctttg ccacgcaagt
    14341 ccgcatcttc atgacgacca aagccagtaa agtagaacgg tttgtggtta atcaggaact
    14401 gttcgccctt cactgccact gaccggatgc cgacgcgaag cgggtagata tcacactctg
    14461 tctggctttt ggctgtgacg cacagttcat agagataacc ttcacccggt tgccagaggt
    14521 gcggattcac cacttgcaaa gtcccgctag tgccttgtcc agttgcaacc acctgttgat
    14581 ccgcatcacg cagttcaacg ctgacatcac cattggccac cacctgccag tcaacagacg
    14641 cgtggttaca gtcttgcgcg acatgcgtca ccacggtgat atcgtccacc caggtgttcg
    14701 gcgtggtgta gagcattacg ctgcgatgga ttccggcata gttaaagaaa tcatggaagt
    14761 aagactgctt tttcttgccg ttttcgtcgg taatcaccat tcccggcggg atagtctgcc
    14821 agttcagttc gttgttcaca caaacggtga tacgtacact tttcccggca ataacatacg
    14881 gcgtgacatc ggcttcaaat ggcgtatagc cgccctgatg ctccatcact tcctgattat
    14941 tgacccacac tttgccgtaa tgagtgaccg catcgaaacg cagcacgata cgctggcctg
    15001 cccaaccttt cggtataaag acttcgcgct gataccagac gttgcccgca taattacgaa
    15061 tatctgcatc ggcgaactga tcgttaaaac tgcctggcac agcaattgcc cggctttctt
    15121 gtaacgcgct ttcccaccaa cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg
    15181 cccacaggcc gtcgagtttt ttgatttcac gggttggggt ttctacagga cggacgagtc
    15241 gtcggttctg taactatcat catcatcata gacacacgaa ataaagtaat cagattatca
    15301 gttaaagcta tgtaatattt acaccataac caatcaatta aaaaatagat cagtttaaag
    15361 aaagatcaaa gctcaaaaaa ataaaaagag aaaagggtcc taaccaagaa aatgaaggag
    15421 aaaaactaga aatttaccct cagatc
//