pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NRXN3-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NRXN3-FLAG              7244 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7244)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 9, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3957
                     /codon_start=1
                     /gene="NRXN3"
                     /gene_synonym="C14orf60"
                     /product="neurexin 3 isoform 5"
                     /label=NRXN3
                     /note="isoform 5 is encoded by transcript variant 
                     7;neurexin III; neurexin-3-alpha"
                     /db_xref="GI:1056849789"
                     /db_xref="CCDS:CCDS81831.1"
                     /db_xref="GeneID:9369"
                     /db_xref="HGNC:HGNC:8010"
                     /db_xref="MIM:600567"
                     /protein_id="NP_001317124.1"
                     /translation="QKLNDNEWHTVRVVRRGKSLKLTVDDDVAEGTMVGDHTRLEFHNI
                     ETGIMTEKRYISVVPSSFIGHLQSLMFNGLLYIDLCKNGDIDYCELKARFGLRNIIADP
                     VTFKTKSSYLSLATLQAYTSMHLFFQFKTTSPDGFILFNSGDGNDFIAVELVKGYIHYV
                     FDLGNGPNVIKGNSDRPLNDNQWHNVVITRDNSNTHSLKVDTKVVTQVINGAKNLDLKG
                     DLYMAGLAQGMYSNLPKLVASRDGFQGCLASVDLNGRLPDLINDALHRSGQIERGCEGP
                     STTCQEDSCANQGVCMQQWEGFTCDCSMTSYSGNQCNDPGATYIFGKSGGLILYTWPAN
                     DRPSTRSDRLAVGFSTTVKDGILVRIDSAPGLGDFLQLHIEQGKIGVVFNIGTVDISIK
                     EERTPVNDGKYHVVRFTRNGGNATLQVDNWPVNEHYPTGNTDNERFQMVKQKIPFKYNR
                     PVEEWLQEKGRQLTIFNTQAQIAIGGKDKGRLFQGQLSGLYYDGLKVLNMAAENNPNIK
                     INGSVRLVGEVPSILGTTQTTSMPPEMSTTVMETTTTMATTTTRKNRSTASIQPTSDDL
                     VSSAECSSDDEDFVECEPSTDKSLSTSIFEGGYKAHAPKWESKDFRPNKVSETSRTTTT
                     SLSPELIRFTASSSSGMVPKLPAGKMNNRDLKPQPDIVLLPLPTAYELDSTKLKSPLIT
                     SPMFRNVPTANPTEPGIRRVPGASEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYR
                     NRDEGSYQVDETRNYISNSAQSNGTLMKEKQQSSKSGHKKQKNKDREYYV"
     misc_feature    3488..3493
                     /label=SDS
     misc_feature    3780..3785
                     /label=SDS
     CDS             3964..4029
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4033..4464
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4177..4182
                     /label=SDS
     repeat_region   4507..4647
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4507..4636
                     /label=AAV2 ITR
     repeat_region   4508..4648
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4722..5177
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5459..5563
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5564..6424
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6595..7183
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaactatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggca gaagctcaat
     1561 gacaacgagt ggcacaccgt tcgggtggtg cggagaggaa aaagccttaa gttaaccgtg
     1621 gatgatgatg tggctgaggg tacaatggtg ggagaccata cccgtttgga gttccacaac
     1681 attgaaacgg gaatcatgac tgagaaacgc tacatctccg ttgtcccctc cagctttatt
     1741 ggccatctgc agagcctcat gtttaatggc cttctctaca ttgacttgtg caaaaatggt
     1801 gacattgatt attgtgagct gaaggctcgt tttggactga ggaacatcat cgctgaccct
     1861 gtcaccttta agaccaagag cagctacctg agccttgcca ctcttcaggc ttacacctcc
     1921 atgcacctct tcttccagtt caagaccacc tcaccagatg gcttcattct cttcaatagt
     1981 ggtgatggca atgacttcat tgcagtcgag cttgtcaagg ggtatataca ctacgttttt
     2041 gacctcggaa acggtcccaa tgtgatcaaa ggcaacagtg accgccccct gaatgacaac
     2101 cagtggcaca atgtcgtcat cactcgggac aatagtaaca ctcatagcct gaaagtggac
     2161 accaaagtgg tcactcaggt tatcaatggt gccaaaaatc tggatttgaa aggtgatctc
     2221 tatatggctg gtctggccca aggcatgtac agcaacctcc caaagctcgt ggcctctcga
     2281 gatggctttc agggctgtct agcatcagtg gacttgaatg gacgcctgcc agacctcatc
     2341 aatgatgctc ttcatcggag cggacagatc gagcgtggct gtgaaggacc cagtaccacc
     2401 tgccaggaag attcatgtgc caaccagggg gtctgcatgc aacaatggga gggcttcacc
     2461 tgtgattgtt ctatgacctc ttattctgga aaccagtgca atgatcctgg cgctacgtac
     2521 atctttggga aaagtggtgg gcttatcctc tacacctggc cagccaatga caggcccagc
     2581 acgcggtctg accgccttgc cgtgggcttc agcaccactg tgaaggatgg catcttggtc
     2641 cgcatcgaca gtgctccagg acttggtgac ttcctccagc ttcacataga acaggggaaa
     2701 attggagttg tcttcaacat tggcacagtt gacatctcca tcaaagagga gagaacccct
     2761 gtaaatgacg gcaaatacca tgtggtacgc ttcaccagga acggcggcaa cgccaccctg
     2821 caggtggaca actggccagt gaatgaacat tatcctacag gcaacactga taatgaacgc
     2881 ttccaaatgg taaaacagaa aatccccttc aaatataatc ggcctgtaga ggagtggctg
     2941 caggaaaaag gccggcagtt aaccatcttc aacactcagg cgcaaatagc cattggtgga
     3001 aaggacaaag gacgcctctt ccaaggccaa ctctctgggc tctattatga tggtttgaaa
     3061 gtactgaaca tggcggctga gaacaacccc aatattaaaa tcaatggaag tgttcggctg
     3121 gttggagaag tcccatcaat tttgggaaca acacagacga cctccatgcc accagaaatg
     3181 tctactactg tcatggaaac cactactaca atggcgacta ccacaacccg taagaatcgc
     3241 tctacagcca gcattcagcc aacatcagat gatcttgttt catctgctga atgttcaagt
     3301 gatgatgaag actttgttga atgtgagccg agtacagata agagtctttc cacttcaatc
     3361 ttcgaaggtg gctacaaagc acatgcgccc aagtgggaat ccaaggactt tagacctaac
     3421 aaagtctccg aaactagtag gactactacc acatctttat cccctgagct gatccgcttc
     3481 acagcttcct cctcgtctgg gatggtgccc aaattgccag ctggcaaaat gaataaccgt
     3541 gatctcaaac cccagcctga tatagtcttg cttccgttgc ccactgccta tgagctagac
     3601 agcaccaaac tgaagagccc actaattact tcccccatgt tccgtaatgt gcccacagca
     3661 aaccccacgg agccgggaat cagacgggtt ccgggggcct cagaggtgat ccgggagtcg
     3721 agcagcacaa cagggatggt cgtcggcatt gtggctgctg ccgccctctg catcttgatc
     3781 ctcctgtacg ccatgtacaa gtacaggaac agggacgagg ggtcctatca agtggacgag
     3841 acgcggaact acatcagcaa ctccgcccag agcaacggca cgctcatgaa ggagaagcag
     3901 cagagctcga agagcggcca caagaaacag aaaaacaagg acagggagta ttacgtgtgt
     3961 acagactaca aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac
     4021 gatgacaagt aactcgagat aatcaacctc tggattacaa aatttgtgaa agattgactg
     4081 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4141 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag
     4201 ttcttgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     4261 tgttgggcac tgacaattcc gtggtgttta tttgtgaaat ttgtgatgct attgctttat
     4321 ttgtaaccat ctagctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     4381 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     4441 ggggagatgt gggaggtttt ttaaagatct ggagaccggt gtggcacgtg cggaccgagc
     4501 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     4561 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     4621 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     4681 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     4741 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt
     4801 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4861 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4921 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4981 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5041 aacaacactc aactctatct cgggctattc ttttgattta taagggattt tgccgatttc
     5101 ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     5161 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     5221 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     5281 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     5341 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     5401 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     5461 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5521 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5581 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5641 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5701 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5761 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5821 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5881 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5941 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     6001 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     6061 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     6121 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     6181 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     6241 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     6301 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6361 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6421 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6481 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6541 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6601 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6661 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6721 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6781 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6841 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6901 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6961 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7021 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7081 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7141 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7201 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//