pYSJ35

LOCUS       pYSJ35            6818 bp DNA     circular SYN 11-DEC-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 5, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6818
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     914..964
                     /label=oYSJ117-M-ETS2-F
     gene            935..2342
                     /gene="Ets2"
                     /gene_synonym="Ets-2"
                     /label=Ets2
                     /note="E26 avian leukemia oncogene 2, 3' domain"
                     /db_xref="GeneID:23872"
                     /db_xref="MGI:MGI:95456"
     CDS             935..2341
                     /codon_start=1
                     /gene="Ets2"
                     /gene_synonym="Ets-2"
                     /product="protein C-ets-2"
                     /label=Ets2
                     /db_xref="CCDS:CCDS37412.1"
                     /db_xref="GeneID:23872"
                     /db_xref="MGI:MGI:95456"
                     /protein_id="NP_035939.3"
                     /translation="MNDFGIKNMDQVAPVANSFRGTLKRQPAFDTFDGSLFAVLPSLSE
                     DQTLQEVPTGLDSVSHDSASCELPLLTPCSKAVMSQALKATFSGFQKEQRRLGIPKNPW
                     LWSEQQVCQWLLWATNEFSLVNVNLHQFGMNGQMLCNLGKERFLELAPDFVGDILWEHL
                     EQMIKENQEKTEDQYEENSHLNAVPHWINSNTLGFSMEQAPYGMQAPNYPKDNLLDSMC
                     PPSATPAALGSELQMLPKSRLNTVNVNYCSISQDFPSSNVNLLNNNSGKPKDHDSPENG
                     GDSFESSDSLLRSWNSQSSLLDVQRVPSFESFEEDCSQSLCLSKLTMSFKDYIQERSDP
                     VEQGKPVIPAAVLAGFTGSGPIQLWQFLLELLSDKSCQSFISWTGDGWEFKLADPDEVA
                     RRWGKRKNKPKMNYEKLSRGLRYYYDKNIIHKTSGKRYVYRFVCDLQNLLGFTPEELHA
                     ILGVQPDTED"
     exon            935..1006
                     /gene="Ets2"
                     /gene_synonym="Ets-2"
                     /inference="alignment:same species:2.1.0"
     exon            1007..1118
                     /gene="Ets2"
                     /gene_synonym="Ets-2"
                     /inference="alignment:same species:2.1.0"
     exon            1119..1238
                     /gene="Ets2"
                     /gene_synonym="Ets-2"
                     /inference="alignment:same species:2.1.0"
     exon            1239..1439
                     /gene="Ets2"
                     /gene_synonym="Ets-2"
                     /inference="alignment:same species:2.1.0"
     exon            1440..1523
                     /gene="Ets2"
                     /gene_synonym="Ets-2"
                     /inference="alignment:same species:2.1.0"
     exon            1524..1742
                     /gene="Ets2"
                     /gene_synonym="Ets-2"
                     /inference="alignment:same species:2.1.0"
     misc_feature    1592..1594
                     /gene="Ets2"
                     /gene_synonym="Ets-2"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P15036; propagated from 
                     UniProtKB/Swiss-Prot (P15037.1); phosphorylation site"
     misc_feature    1607..1609
                     /gene="Ets2"
                     /gene_synonym="Ets-2"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P15036; propagated from 
                     UniProtKB/Swiss-Prot (P15037.1); phosphorylation site"
     misc_feature    1718..1804
                     /gene="Ets2"
                     /gene_synonym="Ets-2"
                     /note="propagated from UniProtKB/Swiss-Prot (P15037.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     exon            1743..2006
                     /gene="Ets2"
                     /gene_synonym="Ets-2"
                     /inference="alignment:same species:2.1.0"
     misc_feature    1814..1816
                     /gene="Ets2"
                     /gene_synonym="Ets-2"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P15036; propagated from 
                     UniProtKB/Swiss-Prot (P15037.1); phosphorylation site"
     misc_feature    1823..1825
                     /gene="Ets2"
                     /gene_synonym="Ets-2"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from 
                     UniProtKB/Swiss-Prot (P15037.1); phosphorylation site"
     misc_feature    1832..1834
                     /gene="Ets2"
                     /gene_synonym="Ets-2"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from 
                     UniProtKB/Swiss-Prot (P15037.1); phosphorylation site"
     exon            2007..2125
                     /gene="Ets2"
                     /gene_synonym="Ets-2"
                     /inference="alignment:same species:2.1.0"
     exon            2126..2342
                     /gene="Ets2"
                     /gene_synonym="Ets-2"
                     /inference="alignment:same species:2.1.0"
     primer_bind     complement(2318..2362)
                     /label=oYSJ118-M-ETS2-R
     polyA_signal    2418..2642
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2688..3116
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3130..3459
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3310..3445
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3526..4320
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4494..4615
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4664..4680)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4688..4704
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4712..4742)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4757..4778
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5066..5651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5822..6682)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6683..6787)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccatgaat gactttggaa tcaagaacat
      961 ggaccaagtg gcccctgtcg ccaacagttt tcgtgggaca ctcaagcgcc agccagcctt
     1021 tgacaccttc gatggctctc tgtttgctgt gctcccttct ctcagtgaag atcagacact
     1081 ccaagaagtg cccacgggcc tggattctgt ctcccatgac tcggccagct gcgagctgcc
     1141 tttgctcact ccctgcagca aggcagtgat gagccaagcc ttaaaagcca ccttcagtgg
     1201 cttccaaaag gagcaacgac gtcttggcat ccccaaaaac ccctggctgt ggagcgagca
     1261 gcaggtgtgc cagtggcttc tctgggccac caacgagttc agcctggtga atgtgaacct
     1321 gcaccagttt ggcatgaacg gccagatgct gtgtaacctc ggcaaggagc gcttcctgga
     1381 gctggcgcct gactttgtgg gtgacatcct ctgggaacat ctagagcaga tgatcaaaga
     1441 gaaccaagaa aagacagaag accaatatga ggaaaactct cacctcaacg cggttcctca
     1501 ttggatcaac agcaatacat taggcttcag catggaacag gctccatatg gaatgcaggc
     1561 accaaactac cccaaagaca atctcctgga cagcatgtgc ccgccatcgg ccacgcctgc
     1621 agctctgggc tctgagctcc agatgttgcc caagtctcgg ctcaacaccg tcaatgtcaa
     1681 ttactgttcc atcagccagg acttccccag cagcaacgtg aatttgctca acaacaattc
     1741 tggaaaaccc aaggaccacg actctccaga gaacggtggg gacagcttcg agagctccga
     1801 ctcgctgctg aggtcctgga acagccagtc gtccctactg gatgtacagc gggtaccttc
     1861 cttcgagagc tttgaggagg actgtagcca gtctctgtgc ctcagtaagc tgaccatgtc
     1921 cttcaaggac tacatccaag agaggagcga cccagtcgag caaggcaaac cagttattcc
     1981 tgcagcagta ctggctggct tcactggaag cggaccaatc cagttgtggc agtttcttct
     2041 ggagctactc tctgacaagt cctgtcaatc tttcatcagc tggacggggg atggatggga
     2101 gttcaagctt gctgaccccg atgaggttgc ccgccggtgg gggaagagga aaaataaacc
     2161 aaagatgaac tacgagaagc tgagccgggg cttacgttac tactacgaca agaacatcat
     2221 ccacaagact tcgggcaagc gctacgtgta ccgtttcgta tgtgacctgc agaacttgct
     2281 gggcttcact ccggaggaac tgcatgccat cctgggcgtc cagcctgata cagaagactg
     2341 agaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac
     2401 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     2461 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     2521 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     2581 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     2641 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     2701 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     2761 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     2821 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     2881 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     2941 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     3001 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     3061 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     3121 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     3181 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     3241 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     3301 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3361 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     3421 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     3481 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     3541 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     3601 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     3661 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     3721 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     3781 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     3841 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     3901 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     3961 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     4021 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     4081 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     4141 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     4201 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     4261 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     4321 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     4381 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     4441 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     4501 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4561 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     4621 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     4681 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     4741 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     4801 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     4861 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     4921 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     4981 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     5041 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     5101 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     5161 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     5221 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     5281 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     5341 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     5401 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     5461 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     5521 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     5581 aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     5641 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     5701 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     5761 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     5821 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5881 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5941 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6001 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6061 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6121 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6181 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6241 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6301 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6361 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6421 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     6481 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6541 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     6601 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     6661 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     6721 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     6781 ttccgcgcac atttccccga aaagtgccac ctgacgtc
//