pYSJ39

LOCUS       pYSJ39               6701 bp DNA     circular SYN 14-DEC-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6701
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     914..953
                     /label=oYSJ125-mELK1-F
     gene            935..2224
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /label=Elk1
                     /note="ELK1, member of ETS oncogene family"
                     /db_xref="GeneID:13712"
                     /db_xref="MGI:MGI:101833"
     CDS             935..2224
                     /codon_start=1
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /product="ETS domain-containing protein Elk-1"
                     /label=Elk1
                     /db_xref="CCDS:CCDS30048.1"
                     /db_xref="GeneID:13712"
                     /db_xref="MGI:MGI:101833"
                     /protein_id="NP_031948.4"
                     /translation="MDPSVTLWQFLLQLLREQGNGHIISWTSRDGGEFKLVDAEEVARL
                     WGLRKNKTNMNYDKLSRALRYYYDKNIIRKVSGQKFVYKFVSYPEVAGCSTEDCPPQPE
                     VSVTSAIAMAPATVHAGPGDTATGKPGTPKGAGMTGQGGLARSSRNEYMRSGLYSTFTI
                     QSLQPQPQPPIPPRPASVLPNTTPAGVPAPASGSRSTSPNPLEACLEAEEAGLPLQVIL
                     TPPEAPNQKSEELSLDPSFGHPQPPEVKVEGPKEELEAARAGGFSSEAVKAEPEVSASE
                     GLLARLPAILTENTAQVCGLSTSTTEITQPQKGRKPRDLELPLSPSLLGGQGPERTPGS
                     GTSSGLQAPGPALTPSLLPTHTLTPVLLTPSSLPPSIHFWSTLSPIAPRSPAKLSFQFP
                     SSGSAQVHIPSISVDGLSTPVVLSPGPQKP"
     exon            935..1144
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /inference="alignment:same species:2.1.0"
     misc_feature    1097..1099
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /note="Region: alternative start codon, AUG sELK-1; start
                     of brain-specific isoform sELK-1; PMID:11050086"
     exon            1145..1591
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /inference="alignment:same species:2.1.0"
     misc_feature    1289..1372
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /note="propagated from UniProtKB/Swiss-Prot (P41969.3);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1427..1546
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /note="propagated from UniProtKB/Swiss-Prot (P41969.3);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     exon            1592..2023
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /inference="alignment:same species:2.1.0"
     misc_feature    1613..1693
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /note="propagated from UniProtKB/Swiss-Prot (P41969.3);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1838..1996
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /note="propagated from UniProtKB/Swiss-Prot (P41969.3);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1907..1909
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /note="Phosphoserine, by MAPK1. 
                     /evidence=ECO:0000250|UniProtKB:P19419; propagated from 
                     UniProtKB/Swiss-Prot (P41969.3); phosphorylation site"
     misc_feature    1943..1945
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /note="Phosphothreonine, by MAPK1. 
                     /evidence=ECO:0000250|UniProtKB:P19419; propagated from 
                     UniProtKB/Swiss-Prot (P41969.3); phosphorylation site"
     misc_feature    1982..2134
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /note="propagated from UniProtKB/Swiss-Prot (P41969.3);
                     Region: Sufficient for interaction with MAD2L2. 
                     /evidence=ECO:0000250|UniProtKB:P19419"
     misc_feature    1994..1996
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /note="Phosphothreonine, by MAPK1. 
                     /evidence=ECO:0000250|UniProtKB:P19419; propagated from 
                     UniProtKB/Swiss-Prot (P41969.3); phosphorylation site"
     exon            2024..2125
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /inference="alignment:same species:2.1.0"
     misc_feature    2024..2026
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /note="Phosphothreonine, by MAPK1. 
                     /evidence=ECO:0000250|UniProtKB:P19419; propagated from 
                     UniProtKB/Swiss-Prot (P41969.3); phosphorylation site"
     misc_feature    2039..2041
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /note="Phosphothreonine, by MAPK1. 
                     /evidence=ECO:0000250|UniProtKB:P19419; propagated from 
                     UniProtKB/Swiss-Prot (P41969.3); phosphorylation site"
     misc_feature    2084..2086
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /note="Phosphoserine, by MAPK1 and MAPK8. 
                     /evidence=ECO:0000250|UniProtKB:P19419; propagated from 
                     UniProtKB/Swiss-Prot (P41969.3); phosphorylation site"
     misc_feature    2102..2104
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /note="Phosphoserine, by MAPK1. 
                     /evidence=ECO:0000250|UniProtKB:P19419; propagated from 
                     UniProtKB/Swiss-Prot (P41969.3); phosphorylation site"
     exon            2126..2224
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /inference="alignment:same species:2.1.0"
     misc_feature    2186..2188
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /note="Phosphothreonine, by MAPK1. 
                     /evidence=ECO:0000250|UniProtKB:P19419; propagated from 
                     UniProtKB/Swiss-Prot (P41969.3); phosphorylation site"
     misc_feature    2201..2203
                     /gene="Elk1"
                     /gene_synonym="Elk-1"
                     /note="Phosphoserine, by MAPK1. 
                     /evidence=ECO:0000250|UniProtKB:P19419; propagated from 
                     UniProtKB/Swiss-Prot (P41969.3); phosphorylation site"
     primer_bind     complement(2207..2245)
                     /label=oYSJ126-mELK1-R
     polyA_signal    2301..2525
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2571..2999
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3013..3342
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3193..3328
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3409..4203
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4377..4498
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4547..4563)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4571..4587
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4595..4625)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4640..4661
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4949..5534)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5705..6565)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6566..6670)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccatggac ccatctgtga cgctgtggca
      961 gtttctgctg cagcttctga gagaacaagg taatggccac atcatctcct ggacctcacg
     1021 ggatggtggt gagttcaagt tggtggatgc agaggaggtg gcccggctat ggggactgcg
     1081 caagaacaag accaacatga attacgacaa gcttagccgg gccttgcggt actactatga
     1141 taagaatatc atccgcaagg tgagcggcca gaagtttgtc tacaagtttg tgtcctaccc
     1201 agaggttgca gggtgctcca ctgaagactg cccaccccag cctgaggtgt ctgtaacctc
     1261 ggccatagcc atggcccctg ctactgtcca tgcaggccca ggggacactg ccactggaaa
     1321 gccaggaaca ccaaagggtg caggaatgac aggccaaggt ggcttagcac gaagcagccg
     1381 gaatgaatac atgcgctcgg gcctctattc taccttcaca atacaatccc tgcagccaca
     1441 gccacagcca cccattcctc ctcggcctgc ctcagtgctt cccaacacta cccctgcagg
     1501 agtaccagca cccgcctcag ggagcaggag caccagtcca aaccccttag aagcctgttt
     1561 ggaagcagaa gaggctggtc tgcccctgca ggttatccta accccaccag aggccccaaa
     1621 ccagaaatcc gaagagttga gtctggaccc aagttttggc catccacagc ccccagaagt
     1681 caaagtggag gggcctaagg aagaattgga agctgcaagg gctggaggct tcagttcaga
     1741 agctgtcaaa gctgaaccag aagtctcagc ctcagaaggc ctgctggctc ggctcccagc
     1801 catcctaaca gagaacacag cccaggtgtg tggcctctcc acttccacca ctgagatcac
     1861 ccaaccgcag aagggccgaa agcctcggga cctggaactt ccacttagcc caagcctgct
     1921 gggtggccag ggacctgaac ggactccagg atcaggaaca agctctggtc ttcaggcacc
     1981 ggggccagcg ctaacgccat ccctgctccc cacacatacc ttgaccccgg tgctgctgac
     2041 acccagctcg ctgcccccta gcatccattt ctggagcact ctgagtccaa ttgcaccccg
     2101 tagtccagcc aagctctcct tccagtttcc gtccagtggc agcgcacagg tgcacatccc
     2161 ttccatcagt gtggatggcc tctcgacccc cgtggtgctc tccccagggc cccagaagcc
     2221 atgagaattc tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta
     2281 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     2341 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     2401 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     2461 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     2521 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     2581 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     2641 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     2701 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     2761 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     2821 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     2881 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     2941 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     3001 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     3061 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     3121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     3181 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3241 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     3301 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     3361 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     3421 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     3481 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     3541 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     3601 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     3661 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     3721 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     3781 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     3841 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     3901 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     3961 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     4021 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     4081 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     4141 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     4201 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     4261 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     4321 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     4381 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4441 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4501 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     4561 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     4621 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     4681 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     4741 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     4801 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     4861 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     4921 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4981 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5041 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5101 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     5161 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5221 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     5281 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5341 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     5401 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     5461 gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     5521 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     5581 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     5641 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     5701 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5761 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5821 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5881 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5941 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     6001 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     6061 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     6121 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     6181 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     6241 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     6301 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     6361 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     6421 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     6481 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     6541 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6601 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6661 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//