PCDH-CMV-mCherry-TRIM2

LOCUS       PCDH-CMV-mCherry-TRIM2          9384 bp DNA     circular SYN 04-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1904..1953
                     /label=Primer 4
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2444..2491)
                     /label=Primer 1
     primer_bind     2466..2500
                     /label=Primer 2
     primer_bind     2466..2492
                     /label=Primer 5
     CDS             2496..3203
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3025..3046
                     /label=mcherry-F
     misc_feature    3204..3232
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     3221..3255
                     /label=TRIM2-F
     misc_feature    3240..5474
                     /label=TRIM2
     primer_bind     3855..3872
                     /label=2-seqF0
     primer_bind     complement(5454..5492)
                     /label=TRIM2-R
     misc_feature    5481..6068
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5952..5963)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6141..6374
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6446..6567
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6586..6721
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6759..6775)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6783..6799
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6807..6837)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6852..6873
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7161..7749)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7920..8780)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8781..8885)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9254..9267)
                     /label=Primer 5
     primer_bind     9359..9375
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
     2521 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2581 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2641 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2701 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2761 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2821 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2881 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2941 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3001 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3061 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3121 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3181 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcggaattca
     3241 tggccagtga aggcaccaac atcccaagtc ctgtggtgcg ccagattgac aagcagtttc
     3301 tgatttgcag tatatgcctg gaacggtaca agaatcccaa ggttctcccc tgtctgcaca
     3361 ctttctgcga gaggtgcctg cagaactaca ttcctgccca cagtttaacc ctctcctgcc
     3421 cagtgtgccg ccagacctcc atcctgcccg agaaaggggt ggccgcgctc cagaacaatt
     3481 tcttcatcac aaacctgatg gacgtgctgc agcgaactcc aggcagcaac gctgaggagt
     3541 cttccatcct ggagacagtc actgctgtgg ctgcgggaaa gcctctctct tgcccaaacc
     3601 acgatgggaa tgtgatggaa ttttactgcc agtcctgtga gactgccatg tgtcgggagt
     3661 gcacggaggg ggagcacgca gagcacccca cagttccact caaggatgtg gtggaacagc
     3721 acaaggcctc gctccaggtc cagctggatg ctgtcaacaa aaggctccca gaaatagatt
     3781 ctgctcttca gttcatctct gaaatcattc atcagttaac caaccaaaag gccagcatcg
     3841 tggatgacat tcattccacc tttgatgagc tccagaagac tttaaatgtg cgcaagagtg
     3901 tgctgcttat ggaattggag gtcaactatg gcctcaaaca caaagtcctc cagtcgcagc
     3961 tggatactct gctccagggg caggagagca ttaagagctg cagcaacttc acagcgcagg
     4021 ccctcaacca tggcacggag accgaggtcc tactggtgaa gaagcagatg agcgagaagc
     4081 tgaacgagct ggccgaccag gacttcccct tgcacccgcg ggagaacgac cagctggatt
     4141 tcatcgtgga aaccgagggg ctgaagaagt ccatccacaa cctcgggacg atcttaacca
     4201 ccaacgccgt tgcctcagag acagtggcca cgggcgaggg gctgcggcag accatcatcg
     4261 ggcagcccat gtccgtcacc atcaccacca aggacaaaga cggtgagctg tgcaaaaccg
     4321 gcaacgccta cctcaccgcc gaactgagca cccccgacgg gagcgtggca gacggggaga
     4381 tcctggacaa caagaacggc acctatgagt ttttgtacac tgtccagaag gaaggggact
     4441 ttaccctgtc tctgagactc tatgaccagc acatccgagg cagcccgttt aagctgaaag
     4501 tgatccgatc cgctgatgtg tctcccacca cagaaggcgt gaagaggcgc gttaagtccc
     4561 cggggagcgg ccacgtcaag cagaaagctg tgaaaagacc cgcaagcatg tacagcactg
     4621 gaaaacgaaa agagaatccc atcgaagacg atttgatctt tcgagtgggt accaaaggaa
     4681 gaaataaagg agagtttaca aatcttcagg gggtagctgc atctacaaat ggaaagatat
     4741 taattgcaga cagtaacaac caatgtgtgc agatattttc caatgatggc cagttcaaaa
     4801 gtcgttttgg catacgggga cgctctccgg ggcagctgca gcggcccaca ggagtggctg
     4861 tacatcccag tggggacata atcattgccg attatgataa taaatgggtc agcattttct
     4921 cctccgatgg gaaatttaag acaaaaattg gatcaggaaa gctgatggga cccaaaggag
     4981 tttctgtgga ccgcaatggg cacattattg ttgtggacaa caaggcgtgc tgcgtgttta
     5041 tcttccagcc aaacgggaaa atagtcacca ggtttggtag ccgaggaaat ggggacaggc
     5101 agtttgcagg tccccatttt gcagctgtaa atagcaataa tgagattatt attacagatt
     5161 tccataatca ttctgtcaag gtgtttaatc aggaaggaga attcatgttg aagtttggct
     5221 caaatggaga aggaaatggg cagtttaatg ctccaacagg tgtagcagtg gattcaaatg
     5281 gaaacatcat tgtggccgac tggggaaaca gcaggatcca ggtttttgat gggagtggat
     5341 catttttgtc ctacattaac acatctgctg acccactcta tggcccccaa ggcctggccc
     5401 taacttcaga tggtcatgtt gtggttgcag actctggaaa tcactgtttc aaagtctatc
     5461 gatacttaca gtaaggatcc aatcaacctc tggattacaa aatttgtgaa agattgactg
     5521 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5581 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5641 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5701 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5761 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5821 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     5881 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5941 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6001 tgcggcctct tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc ctttggccgc
     6061 ctccccgccg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     6121 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct
     6181 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6241 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6301 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6361 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     6421 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     6481 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6541 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     6601 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     6661 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     6721 ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga
     6781 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6841 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6901 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     6961 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7021 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7081 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7141 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7201 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7261 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7321 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7381 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7441 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7501 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7561 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     7621 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7681 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7741 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7801 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7861 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7921 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7981 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8041 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8101 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8161 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     8221 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     8281 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8341 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8401 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     8461 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8521 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     8581 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8641 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8701 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8761 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8821 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8881 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     8941 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     9001 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     9061 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     9121 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     9181 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
     9241 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     9301 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     9361 aaaacgacgg ccagtgccaa gctg
//