| 质粒名称: | pAAVLINK-EF1alpha-HA-5'-ATRX |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001922 |
| 产品用途: | 5' AAVLINK plasmid for ATRX expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1alpha |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ATRX |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1alpha-HA-5'-ATRX
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1alpha-HA-5'-ATRX载体质粒基本信息
pAAVLINK-EF1alpha-HA-5'-ATRX质粒图谱载体图谱和pAAVLINK-EF1alpha-HA-5'-ATRX载体序列质粒序列多克隆位点信息
pAAVLINK-EF1alpha-HA-5'-ATRX质粒载体简介
pAAVLINK-EF1alpha-HA-5'-ATRX质粒序列载体序列
LOCUS pAAVLINK-EF1alpha-HA-5'-ATRX 7341 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7341)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7341
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..4481
/codon_start=1
/gene="ATRX"
/gene_synonym="JMS; MRX52; RAD54; RAD54L; XH2; XNP;ZNF-HX"
/product="transcriptional regulator ATRX isoform 1"
/EC_number="3.6.4.12"
/label=ATRX
/note="isoform 1 is encoded by transcript variant
1;X-linked nuclear protein; transcriptional regulator ATRX;
X-linked helicase II; ATP-dependent helicase ATRX"
/db_xref="GI:530788276"
/db_xref="CCDS:CCDS14434.1"
/db_xref="GeneID:546"
/db_xref="HGNC:HGNC:886"
/db_xref="MIM:300032"
/protein_id="NP_000480.3"
/translation="TAEPMSESKLNTLVQKLHDFLAHSSEESEETSSPPRLAMNQNTDK
ISGSGSNSDMMENSKEEGTSSSEKSKSSGSSRSKRKPSIVTKYVESDDEKPLDDETVNE
DASNENSENDITMQSLPKGTVIVQPEPVLNEDKDDFKGPEFRSRSKMKTENLKKRGEDG
LHGIVSCTACGQQVNHFQKDSIYRHPSLQVLICKNCFKYYMSDDISRDSDGMDEQCRWC
AEGGNLICCDFCHNAFCKKCILRNLGRKELSTIMDENNQWYCYICHPEPLLDLVTACNS
VFENLEQLLQQNKKKIKVDSEKSNKVYEHTSRFSPKKTSSNCNGEEKKLDDSCSGSVTY
SYSALIVPKEMIKKAKKLIETTANMNSSYVKFLKQATDNSEISSATKLRQLKAFKSVLA
DIKKAHLALEEDLNSEFRAMDAVNKEKNTKEHKVIDAKFETKARKGEKPCALEKKDISK
SEAKLSRKQVDSEHMHQNVPTEEQRTNKSTGGEHKKSDRKEEPQYEPANTSEDLDMDIV
SVPSSVPEDIFENLETAMEVQSSVDHQGDGSSGTEQEVESSSVKLNISSKDNRGGIKSK
TTAKVTKELYVKLTPVSLSNSPIKGADCQEVPQDKDGYKSCGLNPKLEKCGLGQENSDN
EHLVENEVSLLLEESDLRRSPRVKTTPLRRPTETNPVTSNSDEECNETVKEKQKLSVPV
RKKDKRNSSDSAIDNPKPNKLPKSKQSETVDQNSDSDEMLAILKEVSRMSHSSSSDTDI
NEIHTNHKTLYDLKTQAGKDDKGKRKRKSSTSGSDFDTKKGKSAKSSIISKKKRQTQSE
SSNYDSELEKEIKSMSKIGAARTTKKRIPNTKDFDSSEDEKHSKKGMDNQGHKNLKTSQ
EGSSDDAERKQERETFSSAEGTVDKDTTIMELRDRLPKKQQASASTDGVDKLSGKEESF
TSLEVRKVAETKEKSKHLKTKTCKKVQDGLSDIAEKFLKKDQSDETSEDDKKQSKKGTE
EKKKPSDFKKKVIKMEQQYESSSDGTEKLPEREEICHFPKGIKQIKNGTTDGEKKSKKI
RDKTSKKKDELSDYAEKSTGKGDSCDSSEDKKSKNGAYGREKKRCKLLGKSSRKRQDCS
SSDTEKYSMKEDGCNSSDKRLKRIELRERRNLSSKRNTKEIQSGSSSSDAEESSEDNKK
KKQRTSSKKKAVIVKEKKRNSLRTSTKRKQADITSSSSSDIEDDDQNSIGEGSSDEQKI
KPVTENLVLSSHTGFCQSS"
misc_feature 2458..2463
/label=SDS
intron 4482..4522
/label=Splicing Donor
misc_feature 4526..4559
/label=lox2272-JT1571
repeat_region 4604..4744
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4604..4733
/label=AAV2 ITR
repeat_region 4605..4745
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4819..5274
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5556..5660
/gene="bla"
/label=AmpR promoter
CDS 5661..6521
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6692..7280
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctac cgctgagccc atgagtgaaa gcaagttgaa
781 tacattggtg cagaagcttc atgacttcct tgcacactca tcagaagaat ctgaagaaac
841 aagttctcct ccacgacttg caatgaatca aaacacagat aaaatcagtg gttctggaag
901 taactctgat atgatggaaa acagcaagga agagggaact agctcttcag aaaaatccaa
961 gtcttcagga tcgtcacgat caaagaggaa accttcaatt gtaacaaagt atgtagaatc
1021 agatgatgaa aaacctttgg atgatgaaac tgtaaatgaa gatgcgtcta atgaaaattc
1081 agaaaatgat attactatgc agagcttgcc aaaaggtaca gtgattgtac agccagagcc
1141 agtgctgaat gaagacaaag atgattttaa agggcctgaa tttagaagca gaagtaaaat
1201 gaaaactgaa aatctcaaaa aacgcggaga agatgggctt catgggattg tgagctgcac
1261 tgcttgtgga caacaggtca atcattttca aaaagattcc atttatagac acccttcatt
1321 gcaagttctt atttgtaaga attgctttaa gtattacatg agtgatgata ttagccgtga
1381 ctcagatgga atggatgaac aatgtaggtg gtgtgcggaa ggtggaaact tgatttgttg
1441 tgacttttgc cataatgctt tctgcaagaa atgcattcta cgcaaccttg gtcgaaagga
1501 gttgtccaca ataatggatg aaaacaacca atggtattgc tacatttgtc acccagagcc
1561 tttgttggac ttggtcactg catgtaacag cgtatttgag aatttagaac agttgttgca
1621 gcaaaataag aagaagataa aagttgacag tgaaaagagt aataaagtat atgaacatac
1681 atccagattt tctccaaaga agactagttc aaattgtaat ggagaagaaa agaaattaga
1741 tgattcctgt tctggctctg taacctactc ttattccgca ctaattgtgc ccaaagagat
1801 gattaagaag gcaaaaaaac tgattgagac cacagccaac atgaactcca gttatgttaa
1861 atttttaaag caggcaacag ataattcaga aatcagttct gctacaaaat tacgtcagct
1921 taaggctttt aagtctgtgt tggctgatat taagaaggct catcttgcat tggaagaaga
1981 cttaaattcc gagtttcgag cgatggatgc tgtaaacaaa gagaaaaata ccaaagagca
2041 taaagtcata gatgctaagt ttgaaacaaa agcacgaaaa ggagaaaaac cttgtgcttt
2101 ggaaaagaag gatatttcaa agtcagaagc taaactttca agaaaacagg tagatagtga
2161 gcacatgcat cagaatgttc caacagagga acaaagaaca aataaaagta ccggtggtga
2221 acataagaaa tctgatagaa aagaagaacc tcaatatgaa cctgccaaca cttctgaaga
2281 tttagacatg gatattgtgt ctgttccttc ctcagttcca gaagacattt ttgagaatct
2341 tgagactgct atggaagttc agagttcagt tgatcatcaa ggggatggca gcagtggaac
2401 tgaacaagaa gtggagagtt catctgtaaa attaaatatt tcttcaaaag acaacagagg
2461 aggtattaaa tcaaaaacta cagctaaagt aacaaaagaa ttatatgtta aactcactcc
2521 tgtttccctt tctaattccc caattaaagg tgctgattgt caggaagttc cacaagataa
2581 agatggctat aaaagttgtg gtctgaaccc caagttagag aaatgtggac ttggacagga
2641 aaacagtgat aatgagcatt tggttgaaaa tgaagtttca ttacttttag aggaatctga
2701 tcttcgaaga tccccacgtg taaagactac acccttgagg cgaccgacag aaactaaccc
2761 tgtaacatct aattcagatg aagaatgtaa tgaaacagtt aaggagaaac aaaaactatc
2821 agttccagtg agaaaaaagg ataagcgtaa ttcttctgac agtgctatag ataatcctaa
2881 gcctaataaa ttgccaaaat ctaagcaatc agagactgtg gatcaaaatt cagattctga
2941 tgaaatgcta gcaatcctca aagaggtgag caggatgagt cacagttctt cttcagatac
3001 tgatattaat gaaattcata caaaccataa gactttgtat gatttaaaga ctcaggcggg
3061 gaaagatgat aaaggaaaaa ggaaacgaaa aagttctaca tctggctcag attttgatac
3121 taaaaagggc aaatcagcta agagctctat aatttctaaa aagaaacgac aaacccagtc
3181 tgagtcttct aattatgact cagaattaga aaaagagata aagagcatga gtaaaattgg
3241 tgctgccaga accaccaaaa aaagaattcc aaatacaaaa gattttgact cttctgaaga
3301 tgagaaacac agcaaaaaag gaatggataa tcaagggcac aaaaatttga agacctcaca
3361 agaaggatca tctgatgatg ctgaaagaaa acaagagaga gagactttct cttcagcaga
3421 aggcacagtt gataaagaca cgaccatcat ggaattaaga gatcgacttc ctaagaagca
3481 gcaagcaagt gcttccactg atggtgtcga taagctttct gggaaagagg agagttttac
3541 ttctttggaa gttagaaaag ttgctgaaac taaagaaaag agcaagcatc tcaaaaccaa
3601 aacatgtaaa aaagtacagg atggcttatc tgatattgca gagaaattcc taaagaaaga
3661 ccagagcgat gaaacttctg aagatgataa aaagcagagc aaaaagggaa ctgaagaaaa
3721 aaagaaacct tcagacttta agaaaaaagt aattaaaatg gaacaacagt atgaatcttc
3781 atctgatggc actgaaaagt tacctgagcg agaagaaatt tgtcattttc ctaagggcat
3841 aaaacaaatt aagaatggaa caactgatgg agaaaagaaa agtaaaaaaa taagagataa
3901 aacttctaaa aagaaggatg aattatctga ttatgctgag aagtcaacag ggaaaggaga
3961 tagttgtgac tcttcagagg ataaaaagag taagaatgga gcatatggta gagagaagaa
4021 aaggtgcaag ttgcttggaa agagttcaag gaagagacaa gattgttcat catctgatac
4081 tgagaaatat tccatgaaag aagatggttg taactcttct gataagagac tgaaaagaat
4141 agaattgagg gaaagaagaa atttaagttc aaagagaaat actaaggaaa tacaaagtgg
4201 ctcatcatca tctgatgctg aggaaagttc tgaagataat aaaaagaaga agcaaagaac
4261 ttcatctaaa aagaaggcag tcattgtcaa ggagaaaaag agaaactccc taagaacaag
4321 cactaaaagg aagcaagctg acattacatc ctcatcttct tctgatatag aagatgatga
4381 tcagaattct ataggtgagg gaagcagcga tgaacagaaa attaagcctg tgactgaaaa
4441 tttagtgctg tcttcacata ctggattttg ccaatcttca ggtaagtatc aaggttacaa
4501 gacaggttta aggagaccaa taagctatta ttcgtatagg atactttata cgaagttatt
4561 aagttatgga gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg
4621 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
4681 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
4741 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
4801 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
4861 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
4921 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
4981 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
5041 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
5101 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
5161 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
5221 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
5281 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
5341 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
5401 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
5461 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
5521 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
5581 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
5641 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
5701 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
5761 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
5821 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
5881 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
5941 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
6001 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
6061 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
6121 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
6181 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
6241 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
6301 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
6361 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
6421 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
6481 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
6541 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
6601 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
6661 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
6721 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
6781 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
6841 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
6901 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
6961 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
7021 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
7081 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
7141 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
7201 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
7261 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
7321 gctggccttt tgctcacatg t
//
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pAAVLINK-EF1alpha-HA-5'-ATRX质粒载体应用举例
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