pmiRFP2-Actin-C-18

LOCUS       pmiRFP2-Actin-C-18               6072 bp DNA     circular SYN 26-MAY-2023
DEFINITION  Vector for fusing photoactivatable mCherry to the N-terminus of a 
            partner protein.
ACCESSION   .
VERSION     .
KEYWORDS    pmiRFP2-Actin-C-18
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Clontech
  TITLE     Direct Submission
  JOURNAL   Exported Jul 1, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6072
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    607..612
                     /label=Kozak
     CDS             613..1551
                     /codon_start=1
                     /label=miRFP2
                     /translation="MVAGHASGSPDFGTASPSDCEHEEIHLAGSIQPHGTLLVVSEPDH
                     RIIQASANAAEFLNLGSVLGIPLAEIDGDLLIKILPHLDLTAEGMPVAVRCRIGNPSTE
                     YDGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDERGHGEVYSEIHVTGLESYFGNRYPSSLVPQMARRLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPTHLQFLKNMGVRATLVVSLVVGGKLWGLV
                     ICHHYLPRFIHFELRAICVLLAEAIATRITAL"
     misc_feature    661..663
                     /label=Feature 3
     unsure          752
                     /label=Val
     unsure          992..993
                     /label=Ser
     unsure          1075..1077
                     /label=Val
     CDS             1213..1215
                     /label=Thr
     unsure          1243
                     /label=Val
     unsure          1277
                     /label=Thr
     CDS             1369..1371
                     /label=Cys
     unsure          1391
                     /label=Asp
     unsure          1414..1416
                     /label=Ala
     CDS             1552..1605
                     /codon_start=1
                     /label=Linker
                     /translation="SGLRSGSGGGSASGGSGS"
     CDS             1606..2727
                     /codon_start=1
                     /label=Actin
                     /translation="DDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG
                     MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLL
                     TEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVP
                     IYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDF
                     EQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSI
                     MKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWI
                     GGSILASLSTFQQMWISKQEYDESGPSIVHRKCF"
     misc_feature    2731..2736
                     /label=MCS
                     /note="multiple cloning site of fluorescent protein 
                     plasmids"
     polyA_signal    2860..2981
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2988..3443)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3470..3574
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3576..3933
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3784..3919
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3968..4762
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    4994..5041
                     /label=HSV TK poly(A) signal
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     rep_origin      5370..5958
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtcgc aggacacgca agtggaagcc ccgactttgg aaccgccagt
      661 ccgagcgact gtgagcacga agaaatccat ctggccggat ccatccagcc acacggaaca
      721 ctgctggtgg tctccgagcc tgaccataga ataattcagg catctgccaa cgccgctgaa
      781 ttcctgaatc tgggaagtgt gctgggaatc cccctggctg agatcgacgg ggatctactg
      841 atcaagattc tgccccacct ggaccttacc gcagaaggaa tgccagtggc cgtcaggtgc
      901 cgcattggaa acccctccac agagtacgac ggactgatgc atcgaccacc tgaaggagga
      961 ctgatcattg agctggaacg ggctgggcca tccattgatc tgagcggaac actggctccc
     1021 gcactggagc gaattcgaac tgcaggatcc ctgagggctc tgtgcgacga tactgtactg
     1081 ctgttccagc agtgtaccgg ctacgaccga gtgatggtct atcggttcga tgaacggggg
     1141 cacggagagg tgtattcaga aattcatgtg actggactgg agagctactt tggcaacagg
     1201 tatcctagct ccctcgtgcc acagatggcc cggcggctgt acgtgagaca gagggtgcgc
     1261 gtcctggtag atgtcaccta tcagcctgtg ccactggagc caagactgag tcccctgact
     1321 ggcagggacc tggatatgtc agggtgtttt ctgcgctcta tgagtcctac tcacctgcag
     1381 ttcctgaaga acatgggcgt gcgggcaaca ctggtggtca gcctggtggt cggaggcaaa
     1441 ctgtgggggc tggtgatctg ccaccattat ctgccacgct tcatccattt tgagctgcga
     1501 gcaatttgtg tgctgctggc cgaagctatc gcaacccgca ttacagccct gtccggactc
     1561 agatctggca gcggtggagg cagcgcatcc ggcggaagcg gaagcgatga tgatatcgcc
     1621 gcgctcgtcg tcgacaacgg ctccggcatg tgcaaggccg gcttcgcggg cgacgatgcc
     1681 ccccgggccg tcttcccctc catcgtgggg cgccccaggc accagggcgt gatggtgggc
     1741 atgggtcaga aggattccta tgtgggcgac gaggcccaga gcaagagagg catcctcacc
     1801 ctgaagtacc ccatcgagca cggcatcgtc accaactggg acgacatgga gaaaatctgg
     1861 caccacacct tctacaatga gctgcgtgtg gctcccgagg agcaccccgt gctgctgacc
     1921 gaggcccccc tgaaccccaa ggccaaccgc gagaagatga cccagatcat gtttgagacc
     1981 ttcaacaccc cagccatgta cgttgctatc caggctgtgc tatccctgta cgcctctggc
     2041 cgtaccactg gcatcgtgat ggactccggt gacggggtca cccacactgt gcccatctac
     2101 gaggggtatg ccctccccca tgccatcctg cgtctggacc tggctggccg ggacctgact
     2161 gactacctca tgaagatcct caccgagcgc ggctacagct tcaccaccac ggccgagcgg
     2221 gaaatcgtgc gtgacattaa ggagaagctg tgctacgtcg ccctggactt cgagcaagag
     2281 atggccacgg ctgcttccag ctcctccctg gagaagagct acgagctgcc tgacggccag
     2341 gtcatcacca ttggcaatga gcggttccgc tgccctgagg cactcttcca gccttccttc
     2401 ctgggcatgg agtcctgtgg catccacgaa actaccttca actccatcat gaagtgtgac
     2461 gtggacatcc gcaaagacct gtacgccaac acagtgctgt ctggcggcac caccatgtac
     2521 cctggcattg ccgacaggat gcagaaggag atcactgccc tggcacccag cacaatgaag
     2581 atcaagatca ttgctcctcc tgagcgcaag tactccgtgt ggatcggcgg ctccatcctg
     2641 gcctcgctgt ccaccttcca gcagatgtgg atcagcaagc aggagtatga cgagtccggc
     2701 ccctccatcg tccaccgcaa atgcttctag ggatccaccg gatctagata actgatcata
     2761 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     2821 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     2881 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     2941 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt
     3001 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     3061 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     3121 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     3181 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     3241 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     3301 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     3361 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     3421 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     3481 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     3541 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
     3601 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3661 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     3721 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     3781 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3841 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     3901 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
     3961 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     4021 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     4081 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     4141 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     4201 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     4261 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     4321 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     4381 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     4441 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     4501 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     4561 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     4621 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     4681 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     4741 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     4801 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     4861 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     4921 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
     4981 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
     5041 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     5101 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     5161 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
     5221 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5281 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5341 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5401 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5461 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     5521 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5581 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5641 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5701 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5761 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5821 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5881 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5941 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6001 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6061 accgccatgc at
//