pBsVchCAST

LOCUS       pBsVchCAST             16191 bp ds-DNA     circular SYN 11-AUG-2023
DEFINITION  Expression of CRISPR-associated transposases, shuttle vector for 
            Bacillus subtilis / E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    pBsVchCAST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16191)
  TITLE     Sheng Yang Plasmids 2023
REFERENCE   2  (bases 1 to 16191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 11, 2023 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..16191
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1221..1243
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1235..1252
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1236..1252
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1295..2377)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKTAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     2339..2358
                     /label=LacI-R
                     /note="LacI, reverse primer"
     protein_bind    2761..2785
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             9694..9705
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        14268..14372
                     /gene="bla"
                     /label=AmpR promoter
     CDS             14373..15233
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14591..14610)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15404..15992
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15893..15912
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16146..16163
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg tacctattaa
       61 tgtatcgtta gaaaaccgac tgtaaaaagt acagtcggca ttatctcata ttataaaagc
      121 cagtcattag gcctatctga caattcctga atagagttca taaacaatcc tgcatgataa
      181 ccatcacaaa cagaatgatg tacctgtaaa gatagcggta aatatattga attaccttta
      241 ttaatgaatt ttcctgctgt aataatgggt agaaggtaat tactattatt attgatattt
      301 aagttaaacc cagtaaatga agtccatgga ataatagaaa gagaaaaagc attttcaggt
      361 ataggtgttt tgggaaacaa tttccccgaa ccattatatt tctctacatc agaaaggtat
      421 aaatcataaa actctttgaa gtcattcttt acaggagtcc aaataccaga gaatgtttta
      481 gatacaccat caaaaattgt ataaagtggc tctaacttat cccaataacc taactctccg
      541 tcgctattgt aaccagttct aaaagctgta tttgagttta tcacccttgt cactaagaaa
      601 ataaatgcag ggtaaaattt atatccttct tgttttatgt ttcggtataa aacactaata
      661 tcaatttctg tggttatact aaaagtcgtt tgttggttca aataatgatt aaatatctct
      721 tttctcttcc aattgtctaa atcaatttta ttaaagttca tttgatatgc ctcctaaatt
      781 tttatctaaa gtgaatttag gaggcttact tgtctgcttt cttcattaga atcaatcctt
      841 ttttaaaagt caatattact gtaacataaa tatatatttt aaaaatatcc cactttatcc
      901 aattttcgtt tgttgaacta atgggtgctt tagttgaaga ataaaagacc acattaaaaa
      961 atgtggtctt ttgtgttttt ttaaaggatt tgagcgtagc gaaaaatcct tttctttctt
     1021 atcttgataa taagggtaac tattgccgtc gtccattccg acagcatcgc cagtcactat
     1081 ggcgtgctgc tagcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     1141 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     1201 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattcga
     1261 gctcaggcct taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     1321 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     1381 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     1441 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     1501 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     1561 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     1621 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     1681 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     1741 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     1801 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     1861 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     1921 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     1981 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     2041 tgtgcaccgc cgttttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     2101 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     2161 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     2221 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     2281 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     2341 catactctgc gacatcgtat aacgttactg gtttcatcaa aatcgtctcc ctccgtttga
     2401 atatttgatt gatcgtaacc agatgaagca ctctttccac tatccctaca gtgttatggc
     2461 ttgaacaatc acgaaacaat aattggtacg tacgatcttt cagccgactc aaacatcaaa
     2521 tcttacaaat gtagtctttg aaagtattac atatgtaaga tttaaatgca accgtttttt
     2581 cggaaggaaa tgatgacctc gtttccaccg gaattagctt ggtaccagct attgtaacat
     2641 aatcggtacg ggggtgaaaa agctaacgga aaagggagcg gaaaagaatg atgtaagcgt
     2701 gaaaaatttt ttatcttatc acttgaaatt ggaagggaga ttctttatta taagaattgt
     2761 ggaattgtga gcggataaca attcccaatt aaaggaggaa ggatcatagg atccgcgaag
     2821 gaaaaaagcg gccgcaagga gatatacata tgtttttgca aagacctaaa ccttacagcg
     2881 atgaaagttt agaaagtttc tttatccgag tggctaacaa aaatggctac ggtgatgtcc
     2941 atcgcttcct agaagccact aaacgattcc ttcaagacat tgaccataat ggctatcaaa
     3001 cctttccgac tgatataact cggataaacc catactcagc taaaaacagt tccagcgcac
     3061 gaactgcgtc attcctgaaa cttgcacaat tgacatttaa tgaaccgcca gagctacttg
     3121 ggttggcaat taacagaaca aacatgaaat actcgccgtc aactagcgcg gttgttagag
     3181 gtgcagaagt ctttcctcgc agtttactac ggacgcactc catcccctgc tgtcctttgt
     3241 gtctgcgaga aaatggctac gcctcctacc tttggcactt tcaggggtac gaatactgcc
     3301 acagccataa cgtaccttta attaccactt gtagctgtgg taaggagttt gactaccgag
     3361 tatctgggtt aaagggcatt tgctgcaaat gcaaggagcc tatcacctta accagcaggg
     3421 agaacggtca tgaggcagcg tgtactgttt caaactggct tgctggccat gaatctaaac
     3481 ctctgccaaa tcttcctaaa agctaccgat ggggtttagt tcattggtgg atgggtatta
     3541 aagatagcga gttcgatcac ttttcgttcg ttcaattttt ctcaaactgg ccaaggtcat
     3601 tccactcgat aatcgaagat gaagtagagt tcaaccttga gcatgctgtt gtcagcacgt
     3661 ctgaattacg actaaaagat cttcttggtc gattgttttt cggttcaatt cggttacctg
     3721 agcggaatct tcaacacaat atcatccttg gtgagcttct ctgctattta gaaaatcgtt
     3781 tatggcaaga caagggatta atcgccaacc tcaaaatgaa cgcgttagag gcgactgtaa
     3841 tgttaaattg tagcctcgat cagattgcat caatggttga acaacgcatc ttgaagccaa
     3901 atcgaaaaag caagcccaac agccctcttg atgttaccga ttatctattt catttcggcg
     3961 atattttctg tctttggtta gctgagttcc aaagcgatga gtttaaccgt tcgttttatg
     4021 tgtcgaggtg gtaaatgcaa actctgaaag aactaatcgc atccaatcct gacgacttaa
     4081 caactgaact taagagagca tttcgtccac tcacaccgca tattgcaatt gatggtaatg
     4141 aacttgacgc actgacgata ttagtcaatt taaccgataa gactgatgat cagaaagacc
     4201 tgctcgatcg agccaaatgc aagcaaaaac ttcgagatga aaaatggtgg gctagctgca
     4261 taaattgcgt taattacaga caaagccata acccaaaatt cccggatata cgttctgaag
     4321 gcgtgattcg aacccaagcc ctgggtgaat taccgagttt cctactctcg tcctcaaaaa
     4381 tcccaccata ccattggtca tatagccatg attcaaaata cgtcaacaaa agcgcattcc
     4441 tcaccaatga gttttgttgg gatggtgaga tctcatgttt gggtgagctt cttaaagatg
     4501 cagatcaccc actttggaat actttgaaaa agttaggttg ttctcaaaaa acttgcaaag
     4561 caatggcaaa acaactagct gatattactc tcacgactat caatgtcaca ctcgcaccaa
     4621 attacctgac tcaaatatct ctccccgata gtgatacatc ctacatttca ctctctcctg
     4681 ttgcatcgct atcgatgcaa agccactttc atcagaggct ccaagatgaa aatcggcata
     4741 gcgcgataac gcggtttagc cgaactacca acatgggggt cactgcgatg acatgtggcg
     4801 gcgcatttag aatgttaaag tctggcgcta agttttccag cccccctcat caccgattaa
     4861 acagtaaacg aagttggttg acgtcagagc atgttcagtc attaaaacag taccagcgcc
     4921 tcaacaaaag cctcatacct gaaaactctc ggattgcact ccgtagaaaa tacaaaatcg
     4981 agcttcaaaa tatggtcaga tcttggtttg caatgcaaga ccataccctt gattcgaaca
     5041 tacttatcca acatttgaat catgacctat cttacttagg agccacaaaa cgttttgcat
     5101 acgatccagc gatgaccaag ctctttactg agcttttgaa acgagagtta tcaaattcaa
     5161 tcaataatgg tgagcaacac actaatggat cgtttttagt cctaccgaat atcagggttt
     5221 gtggcgcaac agctttaagc tccccggtaa cggtggggat tccatcactt acagctttct
     5281 ttggcttcgt tcacgcattt gaacggaata taaatcgcac cacctcatcg tttcgtgttg
     5341 aatcctttgc gatatgcgtc catcaactac atgtcgagaa gcgaggtttg acagcagagt
     5401 ttgtggaaaa aggcgacggg actatatccg ctcccgcgac ccgggatgac tggcagtgtg
     5461 atgtcgtatt tagccttatt ttgaacacca actttgctca acatattgac caagatacgt
     5521 tagttacatc actaccaaag cgattggctc ggggttcagc aaaaattgcg attgatgact
     5581 ttaaacatat caactcattc tcgacattag aaacagcgat cgaatctctg ccaatagaag
     5641 ctggtaggtg gttatcactt tacgcacagt caaacaataa tctaagtgat ctattagcag
     5701 ccatgacaga ggaccatcag ctcatggcaa gctgcgtcgg ttaccacttg ttagaagagc
     5761 ccaaagataa accaaactcc ctcagaggtt acaaacacgc tatcgccgag tgcatcattg
     5821 gactcattaa ctcaatcacc tttagctcag agactgatcc caacacaatc ttttggtcgc
     5881 taaagaacta tcaaaactac ctagtggtac agccaaggag tatcaacgat gaaactaccg
     5941 acaaatctag cctatgagcg ctctatcgac ccatcagatg tctgtttttt tgtcgtctgg
     6001 cccgatgata gaaaaacacc tttaacctac aattctcgta ctctgctcgg gcaaatggaa
     6061 gcggcatcat tagcctatga tgtctcaggt caaccaataa aaagtgccac cgctgaggcg
     6121 ttagctcaag ggaaccctca tcaagttgat ttctgccacg ttccatacgg tgcgagtcat
     6181 attgaatgca gtttctccgt ctcgttttct tctgaactac gtcaaccata taagtgtaac
     6241 tcaagcaaag ttaaacaaac gctagtgcaa ttagtcgagc tctacgaaac gaaaatcggc
     6301 tggactgagc tagcaacccg atatttgatg aacatttgca acggtaaatg gctgtggaaa
     6361 aatacccgta aagcctattg ctggaacatt gtacttacac cttggccttg gaacggggaa
     6421 aaggttggat ttgaagatat tcgtactaac tacacctcac ggcaagactt taaaaataat
     6481 aaaaattggt ctgctatagt tgaaatgatc aaaaccgcat tttctagtac tgatgggctg
     6541 gcgatatttg aagtcagggc caccttgcac ttgccaacga atgctatggt gcggccaagc
     6601 caagttttca cagaaaaaga aagtggcagt aaaagtaaat ctaaaactca aaacagtcga
     6661 gtttttcaga gtacaactat tgatggtgaa cgatcgccaa tactaggggc ctttaaaacg
     6721 ggagcagcta ttgcaaccat tgacgactgg tatcctgaag ccactgagcc actaagggtc
     6781 ggacggtttg gggttcatcg cgaagatgtc acttgctacc gtcatccgtc taccggaaaa
     6841 gattttttct cgatattaca acaagcagag cactatattg aagtgttgag cgccaacaaa
     6901 actcccgctc aagaaactat caacgacatg cactttttaa tggctaacct gattaagggt
     6961 gggatgttcc agcataaagg agactgactg tgaaatggta ttataagaca atcacctttc
     7021 tgccagagtt gtgcaacaac gagtcactgg ctgcaaagtg tctccgcgtt ctgcatggat
     7081 ttaactatca gtatgagaca cgaaatatcg gcgtttcatt tccgctttgg tgtgatgcaa
     7141 cggttggaaa aaagatttca tttgtcagca agaacaagat agaactcgac ttactactta
     7201 aacaacacta tttcgtccaa atggaacaac ttcaatactt tcatatatcc aacactgttc
     7261 tcgtcccaga agattgtaca tacgtttcct ttagacgctg tcaatctata gataagctca
     7321 cagcagcagg gctggcaagg aaaatcagac gcctggagaa acgtgctcta tctagaggcg
     7381 agcaatttga cccatcatct tttgctcaaa aagagcatac tgcaatagcg cactaccact
     7441 cacttgggga gtccagcaaa cagacgaacc gcaactttcg actcaatatc aggatgctct
     7501 cggagcaacc ccgtgagggg aactcgattt ttagtagcta tggcttatca aattcagaaa
     7561 actcgtttca gcctgtaccc ttaatctgat ctagaaaagg aggaaggatc ataccatggc
     7621 gacaagttta cctacgccct cagcaattac gacttcggcg ttagagtatg cattccatac
     7681 tcccgctcgc aatctaacga aatctcgcgg aaaaaacatt catcgttatg tcagtgtaaa
     7741 gatgagtaag aggattacgg tagaatctac tctagagtgt gatgcctgct atcactttga
     7801 ttttgagcca agtattgttc gcttttgcgc tcaaccgatt cgatttttat attatctcaa
     7861 tggtcagtct cactcctatg ttcctgactt tctagttcaa tttgatacca acgagtttgt
     7921 tctatatgaa gtaaagtcag cttatgctaa gaacaaacct gattttgatg ttgaatggga
     7981 ggcgaaagta aaagcagcaa ctgaactagg gctagaattg gagcttgttg aagagagtga
     8041 tattagggat acggttgtat taaataatct taagcgcatg catcgttatg cttcgaaaga
     8101 tgagctgaat aacgtacata actctctctt aaaaataata aagtacaatg gcgcccaatc
     8161 tgcaagatgc ttgggagaac agttgggttt aaaaggccga actgttttac caattttgtg
     8221 cgatttgctg tcaaggtgtt tactcgatac acgtttggat aagcctctat ctcttgaatc
     8281 tcgatttgag ttggccagtt atggctaaga aagggttctc aagtttccat agaaaagcag
     8341 tctcttccca agatacgctt gaatcgatag agcttgtctc tagcgctaat tgtctagaaa
     8401 gtgttacgta tcaagatata tcagcatttc ccgaaacaat tgcggtagag attaatttcc
     8461 gattaagcat tcttcgcttt ttagcgcgaa agtgcgaaac cattgtggcc aaatcaattg
     8521 aaccacatcg tgtagagcta cagcaaaact atagtagaaa aatacccagt gcaataacga
     8581 tatatcgatg gtggcttgct tttcgaaaat cagactacaa ccccattagc ttagcaccta
     8641 atatcaagga tagaggtaat agagagacaa aagtgtcaac agttgttgat tctattatgg
     8701 aacaggcagt tgaaagagtt atatctggac gaaaagtcaa tgttagctct gcatataaac
     8761 gtgttcgacg aaaagttcgt caatacaatc taactcatgg aacgaaatac acgtatccta
     8821 agtacgaatc tgtaagaaag cgagtaaaaa agaaaacccc atttgagtta ttagccgcag
     8881 ggaaagggga gagagtagct aagagagagt ttcgccgaat gggaaaaaag atcctcacgt
     8941 ctagcgtgct agagagggtt gaaatagatc acactgtcgt tgaccttttt gcagtacacg
     9001 aagagtatcg aatcccattg ggccgacctt ggcttactca attggttgat tgttacagta
     9061 aagctgtaat cggtttttat ttaggtttcg agcctcctag ctatgtgtcg gtttcccttg
     9121 cacttaagaa tgcaatacaa cgcaaagatg acttaatctc ctcgtatgaa tcgatcgaga
     9181 atgaatggct atgttatggc atcccagacc tactcgtaac tgataatggt aaagagtttt
     9241 tgtcgaaagc atttgatcaa gcatgtgaat cactattgat caatgtgcat caaaataaag
     9301 ttgagacgcc cgacaacaaa cctcatgttg aacgtaacta cgggactatt aatacttctc
     9361 tgttagacga tttacctggg aaatccttca gccagtacct tcaaagagaa gggtacgact
     9421 ctgtgggaga agctaccctt acactcaatg agattagaga aatttactta atttggttgg
     9481 tggatattta tcataaaaaa cccaatcaga gaggcactaa ttgtcctaat gttgcgtgga
     9541 aaaagggttg tcaagaatgg gaaccagagg agttctctgg ttctaaagac gaattagact
     9601 ttaaatttgc tattgttgat tacaaacaac ttactaaagt agggataact gtctacaaag
     9661 aactgagtta tagcaatgac cgtttagctg aatatagagg gaagaaagga aaccataaag
     9721 ttcagttcaa gtataaccct gagtgtatgg cagttatttg ggtgttggat gaggatatga
     9781 atgagtactt tacagttaat gcgattgact acgaatatgc aagtagagta tcactttggc
     9841 aacataaata taacatgaaa tatcaagcag aactaaattc agcagaatat gatgaggaca
     9901 aggaaattga tgcagaaata aaaattgaag aaatcgcaga tcgttcaatt gttaagacta
     9961 acaaaatcag agctcggagg cgtggcgcta ggcatcaaga gaatagcgca agggctaagt
    10021 caatcagtaa tgcgaacccg gcctcgatac aaaaacatga agatgaaatc gttagtgcag
    10081 ataatgacga ttgggatatt gattatgtct gagaaatcgt caaatgagtg aaacgcgtga
    10141 ggctcgtata tcaagagcta aaagggcatt tgtatccaca ccgagcgtta ggaaaatctt
    10201 gagttacatg gatagatgta gagatctatc agacctagag tctgagccta catgcatgat
    10261 ggtctatggt gcttcgggtg taggtaaaac gaccgtcatc aagaaatact taaatcaaaa
    10321 cagaagagag tccgaagccg ggggcgatat aataccggtt ttgcatattg agttgccaga
    10381 caatgcgaag ccagtagatg cagcaaggga attgctggtt gaaatgggtg acccgctagc
    10441 actttatgaa actgacttag ctagattgac gaaaagactg actgaattaa tccctgcggt
    10501 cggcgtgaag ctgattatta tcgatgagtt ccaacatttg gtggaagaaa ggtcaaatcg
    10561 ggttcttacc caagtaggta attggctaaa aatgatactt aacaaaacga aatgtccaat
    10621 tgttatattt ggtatgccat actcaaaagt tgtactgcaa gcaaactcgc aacttcacgg
    10681 gcgattttcc attcaggttg aactgcgccc ctttagctac cagggaggta gaggtgtatt
    10741 taaaactttt ttggaatacc ttgataaagc cctacctttt gaaaaacagg ctggcttagc
    10801 caacgaaagt ttgcagaaaa aattgtatgc attctctcag ggaaacatgc gttcgttgag
    10861 aaaccttatt tatcaagcat ctatcgaagc aattgataat cagcatgaga cgataaccga
    10921 agaagatttc gtttttgcat cgaagttgac atcgggcgat aaacccaact catggaaaaa
    10981 tccttttgag gagggtgttg aggtaacaga agatatgtta cgaccgccac caaaagatat
    11041 tggttgggag gactatttga gacattcaac cccgagagtg agtaaaccag gtagaaataa
    11101 aaactttttc gaataaccta ggtgcaactg aaaagtttat accttacctg gaacaaatgg
    11161 ttgaaacata cgaggctaat atcggcttat taggaatagt ccctgtacta ataaaatcag
    11221 gtggatcagt tgatcagtat attttggacg aagctcggaa agaatttgga gatgacttgc
    11281 ttaattccac aattaaatta agggaaagaa taaagcgatt tgatgttcaa ggaatcacgg
    11341 aagaagatac tcatgataaa gaagctctaa aactattcaa taaccttaca atggaattga
    11401 tcgaaagggt ggaaggttaa tggtacgaaa attaggggat ctacctagaa agccacaagg
    11461 cgataggtca agcttaaaga acccttacat ggatcttaca gattctgaaa gtaaagaaac
    11521 aacagaggtt aaacaaacag aaccaaaaag aaaaaaagca ttgttgaaaa caatgaaagt
    11581 tgatgtttca atccataata agattaaatc gctgcacgaa attctggcag catccgaagg
    11641 gaattcatat tacttagagg atactattga gagagctatt gataagatgg ttgagacatt
    11701 acctgagagc caaaaaactt tttatgaata tgaattaaaa aaaagaacca acaaaggctg
    11761 agacagactc caaacgagtc tgttttttta aaaaaaatat taggagcatt gaatatatat
    11821 tagagaatta agaaagacat gggaataaaa atattttaaa tccagtaaaa atatgataag
    11881 attatttcag aatatgaaga actctgtttg tttttgatga aaaaacaaac aaaaaaaatc
    11941 cacctaacgg aatctcaatt taactaacag cggccaaact gagaagttaa atttgagaag
    12001 gggaaaaggc ggatttatac ttgtatttaa ctatctccat tttaacattt tattaaaccc
    12061 catacaagtg aaaatcctct tttacactgt tcctttaggt gatcgcggag ggacattatg
    12121 agtgaagtaa acctaaaagg aaatacagat gaattagtgt attatcgaca gcaaaccact
    12181 ggaaataaaa tcgccaggaa gagaatcaaa aaagggaaag aagaagttta ttatgttgct
    12241 gaaacggaag agaagatatg gacagaagag caaataaaaa acttttcttt agacaaattt
    12301 ggtacgcata taccttacat agaaggtcat tatacaatct taaataatta cttctttgat
    12361 ttttggggct attttttagg tgctgaagga attgcgctct atgctcacct aactcgttat
    12421 gcatacggca gcaaagactt ttgctttcct agtctacaaa caatcgctaa aaaaatggac
    12481 aagactcctg ttacagttag aggctacttg aaactgcttg aaaggtacgg ttttatttgg
    12541 aaggtaaacg tccgtaataa aaccaaggat aacacagagg aatccccgat ttttaagatt
    12601 agacgtaagg ttcctttgct ttcagaagaa cttttaaatg gaaaccctaa tattgaaatt
    12661 ccagatgacg aggaagcaca tgtaaagaag gctttaaaaa aggaaaaaga gggtcttcca
    12721 aaggttttga aaaaagagca cgatgaattt gttaaaaaaa tgatggatga gtcagaaaca
    12781 attaatattc cagaggcctt acaatatgac acaatgtatg aagatatact cagtaaagga
    12841 gaaattcgaa aagaaatcaa aaaacaaata cctaatccta caacatcttt tgagagtata
    12901 tcaatgacaa ctgaagagga aaaagtcgac agtactttaa aaagcgaaat gcaaaatcgt
    12961 gtctctaagc cttcttttga tacctggttt aaaaacacta agatcaaaat tgaaaataaa
    13021 aattgtttat tacttgtacc gagtgaattt gcatttgaat ggattaagaa aagatattta
    13081 gaaacaatta aaacagtcct tgaagaagct ggatatgttt tcgaaaaaat cgaactaaga
    13141 aaagtgcaat aaactgctga agtatttcag cagttttttt tatttagaaa tagtgaaaaa
    13201 aatataatca gggaggtatc aatatttaat gagtactgat ttaaatttat ttagactgga
    13261 attaataatt aacacgtaga ctaattaaaa tttaatgagg gataaagagg atacaaaaat
    13321 attaatttca atccctatta aattttaaca agggggggat taaaatttaa ttagaggttt
    13381 atccacaaga aaagacccta ataaaatttt tactagggtt ataacactga ttaatttctt
    13441 aatgggggag ggattaaaat ttaatgacaa agaaaacaat cttttaagaa aagcttttaa
    13501 aagataataa taaaaagagc tttgcgatta agcaaaactc tttacttttt cattgacatt
    13561 atcaaattca tcgatttcaa attgttgttg tatcataaag ttaattctgt tttgcacaac
    13621 cttttcagga atataaaaca catctgaggc ttgttttata aactcagggt cgctaaagtc
    13681 aatgtaacgt agcatatgat atggtatagc ttccacccaa gttagccttt ctgcttcttc
    13741 tgaatgtttt tcatatactt ccatgggtat ctctaaatga ttttcctcat gtagcaaggt
    13801 atgagcaaaa agtttatgga attgatagtt cctctctttt tcttcaactt ttttatctaa
    13861 aacaaacact ttaacatctg agtcaatgta agcataagat gtttttccag tcataatttc
    13921 aatcccaaat cttttagaca gaaattctgg acgtaaatct tttggtgaaa gaattttttt
    13981 atgtagcaat atatccgata cagcaccttc taaaagcgtt ggtgaatagg gcattttacc
    14041 tatctcctct cattttgtgg aataaaaata gtcatattcg tccatctacc tatcctatta
    14101 tcgaacagtt gaacttttta atcaaggatc agtccttttt ttcattattc ttaaactgtg
    14161 ctcttaactt taacaactcg atttgttttt ccagatctcg agggtaacta gcctcgccga
    14221 tcccgcaaga ggcccggcag tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    14281 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
    14341 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    14401 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    14461 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    14521 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    14581 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
    14641 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    14701 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    14761 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    14821 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    14881 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    14941 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    15001 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    15061 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    15121 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    15181 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    15241 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    15301 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    15361 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    15421 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    15481 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    15541 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    15601 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    15661 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    15721 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    15781 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    15841 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    15901 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    15961 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    16021 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    16081 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    16141 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcatg c
//