pCMV-PE5max-EGFP

LOCUS       pCMV-PE5max-EGFP             12891 bp DNA     circular SYN 07-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..12891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        87..466
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        467..670
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     621..641
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     712..731
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        712..730
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             780..800
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             801..4901
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(R221K N394K H840A)
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(1409..1419)
                     /label=OL900
     CDS             4902..5003
                     /label=SGGSx2-sv40NLS-SGGSx2
                     /note="/created_by=chenp"
     CDS             4959..4979
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5004..7034
                     /label=RT codon optimized Genscript
                     /note="/created_by=jessiedavis"
     CDS             7080..7100
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7110..7136
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             7146..7202
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     7146..7170
                     /label=OL899
     primer_bind     7146..7169
                     /label=PE001
     primer_bind     complement(7184..7203)
                     /label=PE002
     CDS             7203..9458
                     /label=MLH1 del754-756 genscript codon
                     /note="/created_by=chenp"
                     /note="/modified_by=chenp"
     primer_bind     9429..9483
                     /label=OL899
     primer_bind     9458..9482
                     /label=PE001
     CDS             9459..9515
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(9497..9523)
                     /label=PE002
     primer_bind     9507..9533
                     /label=PE003
     CDS             9516..10235
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10211..10265)
                     /label=OL900
     primer_bind     complement(10212..10236)
                     /label=PE004
     CDS             10241..10258
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(10281..10298)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10287..10511
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(10582..10598)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10582..10598)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10595..10617)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10606..10622
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10630..10660)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10675..10696
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10813..10830)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10984..11572)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11064..11083)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11743..12603)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12366..12385
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12604..12708)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12787..12806)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
       61 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
      121 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      181 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      241 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      301 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      361 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      421 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      481 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      541 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      601 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      661 aagcagagct ggtttagtga accgtcagat ccgctagaga tccgcggccg ctaatacgac
      721 tcactatagg gagagccgcc accatgaaac ggacagccga cggaagcgag ttcgagtcac
      781 caaagaagaa gcggaaagtc gacaagaagt acagcatcgg cctggacatc ggcaccaact
      841 ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc
      901 tgggcaacac cgaccggcac agcatcaaga agaacctgat cggagccctg ctgttcgaca
      961 gcggcgaaac agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac
     1021 ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg
     1081 acagcttctt ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc
     1141 ggcaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca
     1201 tctaccacct gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct
     1261 atctggccct ggcccacatg atcaagttcc ggggccactt cctgatcgag ggcgacctga
     1321 accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc
     1381 tgttcgagga aaaccccatc aacgccagcg gcgtggacgc caaggccatc ctgtctgcca
     1441 gactgagcaa gagcagaaag ctggaaaatc tgatcgccca gctgcccggc gagaagaaga
     1501 atggcctgtt cggaaacctg attgccctga gcctgggcct gacccccaac ttcaagagca
     1561 acttcgacct ggccgaggat gccaaactgc agctgagcaa ggacacctac gacgacgacc
     1621 tggacaacct gctggcccag atcggcgacc agtacgccga cctgtttctg gccgccaaga
     1681 acctgtccga cgccatcctg ctgagcgaca tcctgagagt gaacaccgag atcaccaagg
     1741 cccccctgag cgcctctatg atcaagagat acgacgagca ccaccaggac ctgaccctgc
     1801 tgaaagctct cgtgcggcag cagctgcctg agaagtacaa agagattttc ttcgaccaga
     1861 gcaagaacgg ctacgccggc tacattgacg gcggagccag ccaggaagag ttctacaagt
     1921 tcatcaagcc catcctggaa aagatggacg gcaccgagga actgctcgtg aagctgaaga
     1981 gagaggacct gctgcggaag cagcggacct tcgacaacgg cagcatcccc caccagatcc
     2041 acctgggaga gctgcacgcc attctgcggc ggcaggaaga tttttaccca ttcctgaagg
     2101 acaaccggga aaagatcgag aagatcctga ccttccgcat cccctactac gtgggccctc
     2161 tggccagggg aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc
     2221 cctggaactt cgaggaagtg gtggacaagg gcgcttccgc ccagagcttc atcgagcgga
     2281 tgaccaactt cgataagaac ctgcccaacg agaaggtgct gcccaagcac agcctgctgt
     2341 acgagtactt caccgtgtat aacgagctga ccaaagtgaa atacgtgacc gagggaatga
     2401 gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga
     2461 ccaaccggaa agtgaccgtg aagcagctga aagaggacta cttcaagaaa atcgagtgct
     2521 tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg ggcacatacc
     2581 acgatctgct gaaaattatc aaggacaagg acttcctgga caatgaggaa aacgaggaca
     2641 ttctggaaga tatcgtgctg accctgacac tgtttgagga cagagagatg atcgaggaac
     2701 ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat gaagcagctg aagcggcgga
     2761 gatacaccgg ctggggcagg ctgagccgga agctgatcaa cggcatccgg gacaagcagt
     2821 ccggcaagac aatcctggat ttcctgaagt ccgacggctt cgccaacaga aacttcatgc
     2881 agctgatcca cgacgacagc ctgaccttta aagaggacat ccagaaagcc caggtgtccg
     2941 gccagggcga tagcctgcac gagcacattg ccaatctggc cggcagcccc gccattaaga
     3001 agggcatcct gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca
     3061 agcccgagaa catcgtgatc gaaatggcca gagagaacca gaccacccag aagggacaga
     3121 agaacagccg cgagagaatg aagcggatcg aagagggcat caaagagctg ggcagccaga
     3181 tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa cgagaagctg tacctgtact
     3241 acctgcagaa tgggcgggat atgtacgtgg accaggaact ggacatcaac cggctgtccg
     3301 actacgatgt ggacgctatc gtgcctcaga gctttctgaa ggacgactcc atcgacaaca
     3361 aggtgctgac cagaagcgac aagaaccggg gcaagagcga caacgtgccc tccgaagagg
     3421 tcgtgaagaa gatgaagaac tactggcggc agctgctgaa cgccaagctg attacccaga
     3481 gaaagttcga caatctgacc aaggccgaga gaggcggcct gagcgaactg gataaggccg
     3541 gcttcatcaa gagacagctg gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc
     3601 tggactcccg gatgaacact aagtacgacg agaatgacaa gctgatccgg gaagtgaaag
     3661 tgatcaccct gaagtccaag ctggtgtccg atttccggaa ggatttccag ttttacaaag
     3721 tgcgcgagat caacaactac caccacgccc acgacgccta cctgaacgcc gtcgtgggaa
     3781 ccgccctgat caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc gactacaagg
     3841 tgtacgacgt gcggaagatg atcgccaaga gcgagcagga aatcggcaag gctaccgcca
     3901 agtacttctt ctacagcaac atcatgaact ttttcaagac cgagattacc ctggccaacg
     3961 gcgagatccg gaagcggcct ctgatcgaga caaacggcga aaccggggag atcgtgtggg
     4021 ataagggccg ggattttgcc accgtgcgga aagtgctgag catgccccaa gtgaatatcg
     4081 tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga gtctatcctg cccaagagga
     4141 acagcgataa gctgatcgcc agaaagaagg actgggaccc taagaagtac ggcggcttcg
     4201 acagccccac cgtggcctat tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca
     4261 agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat catggaaaga agcagcttcg
     4321 agaagaatcc catcgacttt ctggaagcca agggctacaa agaagtgaaa aaggacctga
     4381 tcatcaagct gcctaagtac tccctgttcg agctggaaaa cggccggaag agaatgctgg
     4441 cctctgccgg cgaactgcag aagggaaacg aactggccct gccctccaaa tatgtgaact
     4501 tcctgtacct ggccagccac tatgagaagc tgaagggctc ccccgaggat aatgagcaga
     4561 aacagctgtt tgtggaacag cacaagcact acctggacga gatcatcgag cagatcagcg
     4621 agttctccaa gagagtgatc ctggccgacg ctaatctgga caaagtgctg tccgcctaca
     4681 acaagcaccg ggataagccc atcagagagc aggccgagaa tatcatccac ctgtttaccc
     4741 tgaccaatct gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga
     4801 ggtacaccag caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc
     4861 tgtacgagac acggatcgac ctgtctcagc tgggaggtga ctccggcgga agctctggtg
     4921 gcagcaagcg gaccgccgac ggctctgaat tcgagagccc taagaagaaa agaaaggtga
     4981 gcggaggctc tagcggcgga agcaccctga acattgaaga cgagtataga ctgcatgaaa
     5041 caagcaagga acccgacgtg tccctgggct ccacctggct gtccgacttt ccccaggcct
     5101 gggccgagac aggaggaatg ggcctggccg tgcggcaggc acccctgatc atccctctga
     5161 aggccacctc tacacccgtg agcatcaagc agtaccctat gtctcaggag gccagactgg
     5221 gcatcaagcc tcacatccag aggctgctgg accagggcat cctggtgcca tgccagagcc
     5281 cctggaacac accactgctg cccgtgaaga agccaggcac caatgactat agacccgtgc
     5341 aggatctgag agaggtgaac aagagggtgg aggatatcca ccccaccgtg cccaaccctt
     5401 acaatctgct gtccggcctg cccccttctc accagtggta tacagtgctg gacctgaagg
     5461 atgccttctt ttgtctgaga ctgcacccta ccagccagcc actgttcgcc tttgagtgga
     5521 gggaccctga gatgggcatc tctggccagc tgacctggac acgcctgcct cagggcttca
     5581 agaatagccc aacactgttt aacgaggccc tgcaccgcga cctggcagat ttccggatcc
     5641 agcacccaga tctgatcctg ctgcagtacg tggacgatct gctgctggcc gccaccagcg
     5701 agctggattg ccagcaggga acacgcgccc tgctgcagac cctgggaaac ctgggatata
     5761 gggcatccgc caagaaggcc cagatctgtc agaagcaggt gaagtacctg ggctatctgc
     5821 tgaaggaggg ccagagatgg ctgacagagg ccaggaagga gacagtgatg ggccagccaa
     5881 cacccaagac cccaagacag ctgagggagt tcctgggcaa agcaggattt tgcaggctgt
     5941 tcatcccagg attcgcagag atggcagcac ctctgtaccc actgaccaag ccgggcaccc
     6001 tgtttaattg gggccctgac cagcagaagg cctatcagga gatcaagcag gccctgctga
     6061 cagcaccagc cctgggcctg ccagacctga ccaagccttt cgagctgttt gtggatgaga
     6121 agcagggcta cgccaagggc gtgctgaccc agaagctggg accatggaga cggcccgtgg
     6181 cctatctgtc caagaagctg gacccagtgg cagcaggatg gccaccatgc ctgaggatgg
     6241 tggcagcaat cgccgtgctg acaaaggatg ccggcaagct gaccatggga cagccactgg
     6301 tcatcctggc accacacgca gtggaggccc tggtgaagca gcctccagat cgctggctgt
     6361 ctaacgcccg gatgacacac taccaggccc tgctgctgga caccgatcgc gtgcagtttg
     6421 gccctgtggt ggccctgaat ccagccaccc tgctgcctct gccagaggag ggcctgcagc
     6481 acaactgtct ggacatcctg gcagaggcac acggaacaag gccagacctg accgatcagc
     6541 ccctgcctga cgccgatcac acatggtata ccgatggaag ctccctgctg caggagggcc
     6601 agaggaaggc aggagcagca gtgaccacag agacagaagt gatctgggcc aaggccctgc
     6661 cagcaggcac atccgcccag cgggccgagc tgatcgccct gacccaggcc ctgaagatgg
     6721 ccgagggcaa gaagctgaac gtgtacacag actccagata tgccttcgcc accgcacaca
     6781 tccacggaga gatctacagg cgccggggct ggctgacctc tgagggcaag gagatcaaga
     6841 acaaggatga gatcctggcc ctgctgaagg ccctgtttct gcccaagcgg ctgagcatca
     6901 tccactgtcc tggacaccag aagggacact ccgccgaggc aaggggcaat cggatggccg
     6961 accaggccgc cagaaaggct gctattactg aaactcccga cacttccact ctgctgattg
     7021 aaaactcctc cccttctggc ggctcaaaaa gaaccgccga cggcagcgaa ttcgagtctc
     7081 ccaagaagaa gaggaaagtc ggctctggcc ctgccgctaa gagagtgaag ctggacggaa
     7141 gcggagctac taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac
     7201 ctagcttcgt tgctggagtc atccggagac tggacgagac agtggtgaac agaattgccg
     7261 ccggcgaggt gatccagaga cctgccaatg caataaagga gatgatcgag aactgtctgg
     7321 acgccaagtc cacaagcatt caggtgatcg tgaaggaggg cggactgaag ctgatccaga
     7381 tccaagacaa cggcacaggc atcagaaagg aagatctgga catcgtgtgt gaacggttca
     7441 ccacatctaa gctgcagtct tttgaggatc tggcctctat cagtacctac ggcttcagag
     7501 gcgaggccct ggccagcatc agccacgtgg cccatgtgac catcaccacc aaaaccgccg
     7561 acggcaaatg cgcttatcgc gctagctaca gcgacggcaa gctgaaagcc ccgccaaagc
     7621 cttgcgccgg caaccagggt acacagataa cagtggagga tctgttctac aacatcgcca
     7681 cccggagaaa ggccctgaaa aatcccagcg aggagtacgg caagatcctg gaagtcgtcg
     7741 gcagatactc cgtgcacaac gccggaatca gctttagcgt aaagaagcag ggagaaaccg
     7801 tggccgatgt gcgcaccctg ccaaatgcca gcaccgtgga taacatcaga agcattttcg
     7861 gaaatgccgt gtccagagaa ctgatcgaga tcggctgcga agataagacc ctggctttta
     7921 agatgaacgg ctacatcagc aacgccaatt actctgtgaa gaagtgcatc tttcttctgt
     7981 tcatcaacca cagactggtg gaaagcacca gcctgcggaa agccatcgag acagtgtacg
     8041 ccgcctacct gcctaagaac acccacccct tcctgtacct gagcctcgag atcagccctc
     8101 agaacgtgga cgtcaatgtg catcctacaa agcacgaggt gcacttcctg cacgaggaaa
     8161 gcatcctgga aagagtgcag cagcacattg agagcaagct gctgggctct aacagcagca
     8221 gaatgtactt cacacagacc ctgctgcctg gcctggccgg cccctcaggc gaaatggtta
     8281 agtccacaac ctctctgacc tcatccagca ccagcggttc ttccgataag gtgtacgccc
     8341 accagatggt gcggaccgac tctcgggagc agaagctgga cgcctttctg caacctctga
     8401 gcaaacctct gagctctcag cctcaggcca tcgtgaccga ggacaagaca gatatctcct
     8461 ccggccgtgc cagacagcag gacgaagaaa tgctcgagct gccagctcct gccgaggtgg
     8521 ccgccaagaa ccagagcctg gagggagata ccacaaaggg caccagcgaa atgagcgaga
     8581 agcggggccc tacctccagc aaccccagaa aacggcaccg ggaggacagc gacgtggaaa
     8641 tggtggagga cgacagcaga aaggaaatga cagccgcttg tacccctaga agaagaatca
     8701 tcaacctgac ctccgtgctg agcctgcagg aggagatcaa cgagcagggc cacgaggtgc
     8761 tgagagagat gctgcacaat cacagcttcg tgggctgcgt gaaccctcaa tgggccctgg
     8821 ctcagcatca aacaaagctg tacctgctga acaccaccaa gctgagcgaa gagctgttct
     8881 accagatcct catctacgac ttcgccaact tcggcgtgct acgcctgagc gagcccgccc
     8941 ctctgtttga cctggccatg ctggctctgg atagcccaga aagcggctgg acagaagagg
     9001 acggacctaa agaggggctg gctgaataca tcgtggagtt cctgaagaaa aaggccgaga
     9061 tgctggccga ctacttttct ctggaaatcg acgaggaagg caacctgatc ggcctgcctc
     9121 tgctgatcga taactacgtg cctcccctgg aaggcctgcc catcttcatc ctgagactgg
     9181 ctacagaggt gaactgggac gaggaaaagg aatgcttcga gtctctgagc aaggagtgcg
     9241 ccatgttcta tagcatcaga aaacagtaca tctctgaaga gagcactctg tctggccagc
     9301 agagtgaagt gcccggaagc atccccaaca gctggaagtg gaccgtggaa cacatcgtgt
     9361 acaaggccct gcggagccac attctccctc ctaagcactt caccgaggac ggcaacatcc
     9421 tgcagctggc caacctgccc gacctttata aggttttcgc tactaacttc agcctgctga
     9481 agcaggctgg agacgtggag gagaaccctg gacctatggt gagcaagggc gaggagctgt
     9541 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     9601 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     9661 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     9721 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     9781 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     9841 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     9901 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     9961 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
    10021 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
    10081 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
    10141 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
    10201 ggatcactct cggcatggac gagctgtaca agtaaccggt catcatcacc atcaccattg
    10261 agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
    10321 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
    10381 aaatgagaaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
    10441 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
    10501 gggctctatg gcttctgagg cggaaagaac cagctggggc tcgataccgt cgacctctag
    10561 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
    10621 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctagggtg cctaatgagt
    10681 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
    10741 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
    10801 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    10861 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    10921 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    10981 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    11041 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    11101 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    11161 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    11221 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    11281 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    11341 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    11401 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
    11461 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    11521 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    11581 tttgatcttt tctacggggt ctgacactca gtggaacgaa aactcacgtt aagggatttt
    11641 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    11701 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    11761 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    11821 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    11881 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    11941 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    12001 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    12061 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    12121 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    12181 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    12241 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    12301 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    12361 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    12421 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    12481 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    12541 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    12601 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    12661 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    12721 aaaagtgcca cctgacgtcg acggatcggg agatcgatct cccgatcccc tagggtctac
    12781 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
    12841 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc a
//