| 质粒名称: | pYSJ7 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002552 |
| 产品用途: | Mammalian PhCMV-driven constitutive ELF5 expression vector (PhCMV-ELF5-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | ELF5 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
pYSJ7
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYSJ7载体质粒基本信息
pYSJ7质粒图谱载体图谱和pYSJ7载体序列质粒序列多克隆位点信息
pYSJ7质粒载体简介
pYSJ7质粒序列载体序列
LOCUS pYSJ7 6112 bp DNA circular SYN 07-SEP-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM recombinant plasmid
REFERENCE 1 (bases 1 to 6112)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 3, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6112
/mol_type="other DNA"
/organism="recombinant plasmid"
primer_bind 186..207
/label=olyl419
misc_feature 235..818
/label=P_CMV
/label=nonstandard type: promoter_const
misc_feature 235..614
/label=P_CMV_enhancer
/label=nonstandard type: promoter_element
misc_feature 615..818
/label=P_CMV_IE
/label=nonstandard type: promoter_element
primer_bind 769..789
/label=oMHS-CMV-F
misc_feature 829
/label=putative transcriptional start
promoter 863..882
/label=T7 promoter
primer_bind complement(871..894)
/label=olyl418
primer_bind 881..927
/label=QL327 ELF5-F
primer_bind 881..882
/label=T7
CDS 907..1674
/codon_start=1
/label=ELF5
/translation="MLDSVTHSTFLPNASFCDPLMSWTDLFSNEEYYPAFEHQTACDSY
WTSVHPEYWTKRHVWEWLQFCCDQYKLDTNCISFCNFNISGLQLCSMTQEEFVEAAGLC
GEYLYFILQNIRTQGYSFFNDAEESKATIKDYADSNCLKTSGIKSQDCHSHSRTSLQSS
HLWEFVRDLLLSPEENCGILEWEDREQGIFRVVKSEALAKMWGQRKKNDRMTYEKLSRA
LRYYYKTGILERVDRRLVYKFGKNAHGWQEDKL"
primer_bind 1379..1400
/label=QL299 F
primer_bind complement(1456..1477)
/label=QL300 R
primer_bind complement(1654..1694)
/label=QL328 ELF5-R
terminator 1709..1936
/label=bGH polyA
rep_origin 1982..2410
/label=f1 bacteriophage origin of replication
/label=f1 bacteriophage origin of replication; arrow _f1
ori
misc_feature 2424..2753
/label=P_SV40
/label=nonstandard type: promoter_const
rep_origin 2604..2739
/label=SV40 ORI
misc_feature 2656..2705
/label=P_SV40 TSS-region
CDS 2820..3614
/label=Translation 2136-2930
misc_feature 2820..3611
/label=Kan/neoR
/label=nonstandard type: resistance
primer_bind complement(3954..3974)
/label=M13-rev
misc_binding complement(3980..4002)
/label=LacO
promoter complement(4006..4036)
/label=lac promoter
rep_origin complement(4360..4945)
/direction=LEFT
/label=high-copy-number ColE1/pMB1/pBR322/pUC origin o
/label=high-copy-number ColE1/pMB1/pBR322/pUC origin of
_ori
CDS complement(5116..5976)
/label=Translation 5292-4432
misc_feature complement(5119..5976)
/label=AmpR
/label=nonstandard type: resistance
promoter complement(5977..6081)
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gccaccatgt tggactcggt gacacacagc accttcctgc ctaatgcatc cttctgcgat
961 cccctgatgt cgtggactga tctgttcagc aatgaagagt actaccctgc ctttgagcat
1021 cagacagcct gtgactcata ctggacatca gtccaccctg aatactggac taagcgccat
1081 gtgtgggagt ggctccagtt ctgctgcgac cagtacaagt tggacaccaa ttgcatctcc
1141 ttctgcaact tcaacatcag tggcctgcag ctgtgcagca tgacacagga ggagttcgtc
1201 gaggcagctg gcctctgcgg cgagtacctg tacttcatcc tccagaacat ccgcacacaa
1261 ggttactcct tttttaatga cgctgaagaa agcaaggcca ccatcaaaga ctatgctgat
1321 tccaactgct tgaaaacaag tggcatcaaa agtcaagact gtcacagtca tagtagaaca
1381 agcctccaaa gttctcatct atgggaattt gtacgagacc tgcttctatc tcctgaagaa
1441 aactgtggca ttctggaatg ggaagatagg gaacaaggaa tttttcgggt ggttaaatcg
1501 gaagccctgg caaagatgtg gggacaaagg aagaaaaatg acagaatgac atatgaaaag
1561 ttgagcagag ccctgagata ctactataaa acaggaattt tggagcgggt tgaccgaagg
1621 ttagtgtaca aatttggaaa aaatgcacac gggtggcagg aagacaagct atgatctaga
1681 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
1741 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
1801 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
1861 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
1921 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
1981 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
2041 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
2101 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
2161 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
2221 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
2281 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
2341 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
2401 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
2461 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
2521 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
2581 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
2641 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
2701 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
2761 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
2821 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
2881 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
2941 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
3001 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
3061 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
3121 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
3181 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
3241 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
3301 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
3361 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
3421 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
3481 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
3541 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
3601 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
3661 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
3721 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
3781 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
3841 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
3901 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
3961 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
4021 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
4081 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
4141 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
4201 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
4261 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
4321 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
4381 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
4441 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
4501 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
4561 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
4621 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
4681 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
4741 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
4801 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
4861 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat
4921 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
4981 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
5041 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
5101 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
5161 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
5221 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
5281 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
5341 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
5401 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
5461 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
5521 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
5581 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
5641 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
5701 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
5761 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
5821 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
5881 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
5941 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
6001 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
6061 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
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