| 质粒名称: | Xlone-Gem(1-100)-mScarlet(R198Q)-IRES-hCdt1(1-100)(cy-)-mScarlet(Y84W)-IRES-H1.0-mScarlet(M164L) |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001817 |
| 产品用途: | For constructing a tr-Fucci(CA)-red+H1.0 stably transfected cell line in mammalian cells. |
| 存储实验室: | Xin Zhang Lab |
|
载体骨架基本信息 |
|
| 载体名称: | XLone |
| 表达系统: | 哺乳动物表达 |
| 启动子: | TRE3GS |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | Gem(1-100)-mScarlet(R198Q), hCdt1(1-100)(cy-)-mScarlet(Y84W), H1.0-mScarlet(M164L) |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Time-resolved fluorescent proteins expand fluorescent microscopy in temporal and spectral domains.
Tan Z., Hsiung C-H., Feng J., Zhang Y., Wan Y., Chen J., Sun K., Lu P., Zang J., Yang W., Gao Y., Yin J., Zhu T., Lu Y., Pan Z., Zou Y., Liao C., Li X., Ye Y., Liu Y., Zhang X.
Cell 0, (2025)
|
Xlone-Gem(1-100)-mScarlet(R198Q)-IRES-hCdt1(1-100)(cy-)-mSca
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
Xlone-Gem(1-100)-mScarlet(R198Q)-IRES-hCdt1(1-100)(cy-)-mSca载体质粒基本信息
Xlone-Gem(1-100)-mScarlet(R198Q)-IRES-hCdt1(1-100)(cy-)-mSca质粒图谱载体图谱和Xlone-Gem(1-100)-mScarlet(R198Q)-IRES-hCdt1(1-100)(cy-)-mSca载体序列质粒序列多克隆位点信息
载体图谱.png)
Xlone-Gem(1-100)-mScarlet(R198Q)-IRES-hCdt1(1-100)(cy-)-mSca质粒载体简介
Xlone-Gem(1-100)-mScarlet(R198Q)-IRES-hCdt1(1-100)(cy-)-mSca质粒序列载体序列
LOCUS Xlone-Gem(1-100)-mScarlet(R198Q)-IRES-hCdt1(1-100)(cy-)-mScarlet(Y84W)-IRES-H1.0-mScarlet(M164L) 10295 bp DNA circular SYN 28-JUL-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10295)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Aug 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10295
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 9..376
/label=TRE3GS promoter
/note="3rd-generation Tet-responsive promoter that can be
activated by binding of Tet-On(R) 3G, modified to eliminate
binding sites for endogenous mammalian transcription
factors"
protein_bind 17..35
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 53..71
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 89..107
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 125..143
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 161..179
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 197..215
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 233..251
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
CDS 409..1101
/codon_start=1
/product="bright monomeric red fluorescent protein evolved
from a synthetic template (Bindels et al., 2016)"
/label=mScarlet
/translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDQKLDITSHNEDYTVVEQYERSEGRHS
TGGMDELYK"
misc_feature 796..801
/label=SDS
CDS 1132..1461
/codon_start=1
/product="degron consisting of residues 1-110 of human
Geminin (Zielke and Edgar, 2015)"
/label=Gem1 (1-110)
/translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
KRRKAL"
misc_feature 2963..2968
/label=SDS
misc_feature 3143..3729
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 5158..5163
/label=SDS
polyA_signal 5257..5391
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5712..5728)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 6202..6306
/gene="bla"
/label=AmpR promoter
CDS 6307..7167
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
misc_feature 7168..8108
/label=ColE1
/note="ColE1"
rep_origin 7338..7926
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 8214..8235
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8250..8280
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8288..8304
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8312..8328
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
polyA_signal 8592..8673
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS complement(8790..9188)
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
CDS complement(9189..9245)
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS complement(9255..9998)
/codon_start=1
/product="modified rtTA protein that binds tightly to
promoters containing the tet operator in the presence of
doxycycline"
/label=Tet-On(R) 3G
/translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
DALDDFDLDMLPADALDDFDLDMLPG"
CDS complement(9999..10022)
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
regulatory 10022..10031
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
promoter complement(10051..10262)
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
ORIGIN
1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
241 agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg
301 attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
361 ttcctaccct cgtaaaggta ccttggtacc gagctcggat ccgccaccgt gagcaagggc
421 gaggcagtga tcaaggagtt catgcggttc aaggtgcaca tggagggctc catgaacggc
481 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
541 aagctgaagg tgaccaaggg tggccccctg cccttctcct gggacatcct gtcccctcag
601 ttcatgtacg gctccagggc cttcaccaag caccccgccg acatccccga ctactataag
661 cagtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgcc
721 gtgaccgtga cccaggacac ctccctggag gacggcaccc tgatctacaa ggtgaagctc
781 cgcggcacca acttccctcc tgacggcccc gtaatgcaga agaagacaat gggctgggaa
841 gcgtccaccg agcggttgta ccccgaggac ggcgtgctga agggcgacat taagatggcc
901 ctgcgcctga aggacggcgg ccgctacctg gcggacttca agaccaccta caaggccaag
961 aagcccgtgc agatgcccgg cgcctacaac gtcgaccaga agttggacat cacctcccac
1021 aacgaggact acaccgtggt ggaacagtac gaacgctccg agggccgcca ctccaccggc
1081 ggcatggacg agctgtacaa gggctacccc agccactggc ggcctctgga aatgaacccc
1141 agcatgaagc agaagcagga agagatcaaa gagaacatca agaacagcag cgtgcccagg
1201 cggaccctga agatgatcca gcccagcgcc agcggatctc tggtgggccg ggagaatgag
1261 ctgtctgccg gcctgagcaa gcggaagcac cggaacgacc acctgaccag caccacctct
1321 agcccaggcg tgatcgtgcc cgagagcagc gagaacaaga acctgggcgg cgtgacccaa
1381 gaaagcttcg acctgatgat caaagaaaac cccagcagcc agtactggaa agaggtggcc
1441 gagaagcggc ggaaggccct gtaaggtacc gagctcggat cctactccgg tattgcggta
1501 cccttgtacg cctgttttat actcccttcc cgtaacttag acgcacaaaa ccaagttcaa
1561 tagaaggggg tacaaaccag taccaccacg aacaagcact tctgtttccc cggtgatgtc
1621 gtatagactg cttgcgtggt tgaaagcgac ggatccgtta tccgcttatg tacttcgaga
1681 agcccagtac cacctcggaa tcttcgatgc gttgcgctca gcactcaacc ccagagtgta
1741 gcttaggctg atgagtctgg acatccctca ccggtgacgg tggtccaggc tgcgttggcg
1801 gcctacctat ggctaacgcc atgggacgct agttgtgaac aaggtgtgaa gagcctattg
1861 agctacataa gaatcctccg gcccctgaat gcggctaatc ccaacctcgg agcaggtggt
1921 cacaaaccag tgattggcct gtcgtaacgc gcaagtccgt ggcggaaccg actactttgg
1981 gtgtccgtgt ttccttttat tttattgtgg ctgcttatgg tgacaatcac agattgttat
2041 cataaagcga attggatact agtccagtgt ggtggaaatg gtcagtaaag gggaagccgt
2101 cattaaggag tttatgcgct tcaaggttca tatggaaggt agtatgaatg gccacgaatt
2161 cgagattgag ggggaggggg aggggagacc atacgagggg actcagacag ctaaactgaa
2221 ggtgactaag ggcggaccat tgcccttctc atgggacatc ctgagtcctc agtttatgta
2281 tggatcccgg gcattcacaa aacatcctgc tgatatccca gactattgga aacagagctt
2341 ccccgaaggt tttaagtggg aaagagtgat gaacttcgaa gatggtgggg ccgtcactgt
2401 cactcaagac acctctctgg aagacggcac cctgatctac aaagttaaac tgcggggaac
2461 taacttccct ccagatgggc ccgtcatgca gaaaaaaacc atgggatggg aggcaagcac
2521 cgaaagactg tatccagagg acggagttct gaaaggcgat atcaagatgg ccctccgctt
2581 gaaggacgga ggaagatatc tggcagactt caaaactacc tacaaagcaa aaaagcctgt
2641 ccagatgcct ggtgcataca atgtcgacag aaagttggac atcactagtc ataacgaaga
2701 ctatacagtc gttgagcagt acgagaggag cgaggggagg cactccaccg ggggtatgga
2761 tgagctgtat aaggggcatg gcacgggctc gggcaccgcc tcgtcgatgg aacagagaag
2821 agtgaccgac tttttcgcca ggagaagacc tggcccaccc cggatcgccc ctccaaaact
2881 ggcctgcaga accccctctc ctgccagacc tgccctgaga gcccctgcct ccgccacctc
2941 cggcagcaga aagagagcca gacctcctgc cgcccctggc agagatcagg ccaggccccc
3001 tgcccgcgcg gccgcgcggc tgtccgtgga tgaggtgtcc agccctagca cccccgaggc
3061 ccccgatatc cctgcctgtc ccagccctgg ccagaaaatc aagaagtaag tggcggtgaa
3121 gaggcacttt taagcggccg cgcccctctc cctccccccc ccctaacgtt actggccgaa
3181 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
3241 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
3301 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
3361 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
3421 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
3481 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
3541 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
3601 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
3661 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
3721 gccacaaccc cgctcgagat gaccgagaac tccacctccg ccccggcggc gaagcccaaa
3781 cgggccaagg cttccaagaa gtccacggac caccccaagt attcagacat gatcgtggct
3841 gctatccagg cagagaagaa ccgtgccggc tcctcgcgcc agtccatcca aaagtatatc
3901 aagagccact acaaggtggg tgagaacgcc gactcccaga tcaagttgtc catcaagcgc
3961 ctagtgacca ccggtgttct caagcaaacc aaaggggtgg gcgcctcggg gtccttcagg
4021 ctggccaagg gcgatgagcc caaaaggtcg gtggctttca agaagaccaa gaaggaagtc
4081 aagaaagtgg ccactccaaa gaaggcagcc aagcccaaga aggctgcctc caaagcccca
4141 agcaagaaac ccaaagccac ccctgtcaag aaggccaaga agaagccggc tgccacgccc
4201 aagaaagcca aaaagcccaa ggttgtcaaa gtcaaaccag tcaaggcctc caaacccaag
4261 aaggccaaaa ccgtgaagcc caaagccaag tcgagtgcca agagggggag caagaagaag
4321 tccggactca gatctggagg ctccggaggc atggtgtcaa aaggcgaggc agtgattaag
4381 gaattcatgc ggttcaaagt tcacatggaa ggatcaatga atggacacga attcgagatc
4441 gagggcgagg gcgagggccg cccttacgag ggtacccaaa ccgctaagct taaggtcacc
4501 aagggaggcc cactcccctt ctcttgggat attctgtctc cccagtttat gtatgggagt
4561 agagctttca caaagcatcc tgccgacatc cccgactact ataagcaatc ttttcctgag
4621 ggttttaaat gggaacgcgt tatgaatttt gaagacggcg gggccgtgac agtcactcaa
4681 gacacctcac tcgaagacgg aaccctcatc tacaaggtca agcttcgggg gactaatttc
4741 ccccctgacg gcccagtgat gcagaagaaa actatgggat gggaggctag cactgaacgg
4801 ttgtaccccg aggatggcgt gttgaaggga gacatcaaac ttgcacttcg cctcaaagac
4861 ggcggaagat atctcgccga tttcaaaacc acatataagg ctaaaaagcc tgtgcagatg
4921 ccaggggctt ataacgtgga tcgcaagctg gatattacta gtcataacga agattataca
4981 gttgttgagc agtatgaaag gtcagaggga cggcatagca ctgggggaat ggatgagctt
5041 tataaataag aattctgcag atatccagca cagtggacta gtagaccacc tcccctgcga
5101 gctaagctgg acagccaatg acgggtaaga gagtgacatt tttcactaac ctaagacagg
5161 agggccgtca gagctactgc ctaatccaaa gacgggtaaa agtgataaaa atgtatcact
5221 ccaacctaag acaggcgcag cttccgaggg atttgagatc cagacatgat aagatacatt
5281 gatgagtttg gacaaaccaa aactagaatg cagtgaaaaa aatgccttat ttgtgaaatt
5341 tgtgatgcta ttgccttatt tgtaaccatt ataagctgca ataaacaagt ttgatatcta
5401 taacaagaaa atatatatat aataagttat cacgtaagta gaacatgaaa taacaatata
5461 attatcgtat gagttaaatc ttaaaagtca cgtaaaagat aatcatgcgt cattttgact
5521 cacgcggtcg ttatagttca aaatcagtga cacttaccgc attgacaagc acgcctcacg
5581 ggagctccaa gcggcgactg agatgtccta aatgcacagc gacggattcg cgctatttag
5641 aaagagagag caatatttca agaatgcatg cgtcaatttt acgcagacta tctttctagg
5701 gttaagaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
5761 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
5821 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
5881 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
5941 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
6001 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
6061 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
6121 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
6181 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
6241 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
6301 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
6361 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
6421 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
6481 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
6541 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
6601 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
6661 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
6721 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
6781 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
6841 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
6901 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
6961 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
7021 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
7081 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
7141 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
7201 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
7261 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
7321 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
7381 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
7441 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
7501 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
7561 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
7621 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
7681 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
7741 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
7801 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
7861 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
7921 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
7981 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
8041 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
8101 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
8161 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
8221 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
8281 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
8341 aaggtcgact taaccctaga aagataatca tattgtgacg tacgttaaag ataatcatgc
8401 gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag gtttatttat taatttgaat
8461 agatattaag ttttattata tttacactta catactaata ataaattcaa caaacaattt
8521 atttatgttt atttatttat taaaaaaaaa caaaaactca aaatttcttc tataaagtaa
8581 caaaactttt agcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
8641 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
8701 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
8761 ggtatggctg attatgatcc tctggagatt tagccctccc acacataacc agagggcagc
8821 aattcacgaa tcccaactgc cgtcggctgt ccatcactgt ccttcactat ggctttgatc
8881 ccaggatgca gatcgagaag cacctgtcgg caccgtccgc aggggctcaa gatgcccctg
8941 ttctcatttc cgatcgcgac gatacaagtc aggttgccag ctgccgcagc agcagcagtg
9001 cccagcacca cgagttctgc acaaggtccc ccagtaaaat gatatacatt gacaccagtg
9061 aagatgcggc cgtcgctaga gagagctgcg ctggcgacgc tgtagtcttc agagatgggg
9121 atgctgttga ttgtagccgt tgctctttca atgagggtgg attcttcttg agacaaaggc
9181 ttggccatgg ggccggggtt ctcctccacg tcgccggcct gcttcagcag gctgaagttg
9241 gtggcgccgc tgcccccggg gagcatgtca aggtcaaaat cgtcaagagc gtcagcaggc
9301 agcatatcaa ggtcaaagtc gtcaagggca tcggctggga gcatgtctaa gtcaaaatcg
9361 tcaagggcgt cggtcggccc gccgctttcg cactttagct gtttctccag gccacatatg
9421 attagttcca ggccgaaaag gaaggcaggt tcggctccct gccggtcgaa cagctcaatt
9481 gcttgtttca gaagtggggg catagaatcg gtggtaggtg tctctctttc ctcttttgct
9541 acttgatgct cctgttcctc caatacgcag cccagtgtaa agtggcccac ggcggacaga
9601 gcgtacagtg cgttctccag ggagaagcct tgctgacaca ggaacgcgag ctgattttcc
9661 agggtttcgt actgtttctc tgttgggcgg gtgccgagat gcactttagc cccgtcgcga
9721 tgtgagagga gagcacagcg gtatgacttg gcgttgttcc gcagaaagtc ttgccatgac
9781 tcgccttcca gggggcagga gtgggtatga tgcctgtcca gcatctcgat tggcagggca
9841 tcgagcaggg cccgcttgtt cttcacgtgc cagtacaggg taggctgctc aactcccagc
9901 ttttgagcga gtttccttgt cgtcaggcct tcgataccga ctccattgag taattccaga
9961 gcagagttta tgactttgct cttgtccagt ctagacatct tatcgtcatc gtctttgtaa
10021 tccatggtgg cggatcccgc gtcacgacac ctgtgttctg gcggcaaacc cgttgcgaaa
10081 aagaacgttc acggcgacta ctgcacttat atacggttct cccccaccct cgggaaaaag
10141 gcggagccag tacacgacat cactttccca gtttaccccg cgccaccttc tctaggcacc
10201 ggttcaattg ccgacccctc cccccaactt ctcggggact gtgggcgatg tgcgctctgc
10261 ccactgacgg gcaccggagc cactcgagtg gaatt
//
