pYK480

LOCUS      pYK480           11944 bp DNA     circular SYN 15-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 28, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    232..819
                     /label=CMV
     primer_bind     863..882
                     /label=T7
     primer_bind     881..919
                     /label=oYK207_dcas9_F
     CDS             920..5033
                     /codon_start=1
                     /label=Translation 748-6546
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNSKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGDSRA"
     CDS             920..5023
                     /label=dCas9 (D839A and N863A nonsense-mutation)
     primer_bind     complement(5015..5052)
                     /label=oYK480_Cas9_R
     primer_bind     5033..5106
                     /label=oSCL1435
     primer_bind     5033..5064
                     /label=oSCL1439
     primer_bind     5033..5057
                     /label=oYK480_NS3_F
     misc_feature    5039..5071
                     /label=NS4A/NS4B site
     CDS             5039
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation=""
     primer_bind     complement(5043..5065)
                     /label=oSCL1436
     primer_bind     5044..5064
                     /label=oSCL1430
     primer_bind     5045..5102
                     /label=oSCL1387
     primer_bind     5045..5064
                     /label=oSCL1386
     misc_feature    5062^5063
                     /label=cutting site
     primer_bind     5086..5144
                     /label=oSCL1388
     CDS             5090..5125
                     /codon_start=1
                     /label=NS4A cofactor
                     /translation="TGCVVIVGRIVL"
     primer_bind     5126..5145
                     /label=SMASH-4
     primer_bind     5128..5186
                     /label=SMASH-5
     CDS             5144..5701
                     /codon_start=1
                     /label=NS3 protease
                     /translation="APITAYAQQTRGLLGCIITSLTGRDKNQVEGEVQIVSTATQTFLA
                     TCINGVCWAVYHGAGTRTIASPKGPVIQMYTNVDQDLVGWPAPQGSRSLTPCTCGSSDL
                     YLVTRHADVIPVRRRGDSRSSLLSPRPISYLKGSSGGPLLCPAGHAVGLFRAAVCTRGV
                     AKAVDFIPVENLETTMRSPVFTD"
     primer_bind     5166..5224
                     /label=SMASH-6
     primer_bind     5198..5256
                     /label=SMASH-7
     primer_bind     complement(5237..5295)
                     /label=SMASH-8
     primer_bind     complement(5276..5334)
                     /label=SMASH-9
     primer_bind     complement(5318..5376)
                     /label=SMASH-10
     primer_bind     complement(5354..5425)
                     /label=SMASH-11
     primer_bind     complement(5408..5466)
                     /label=SMASH-12
     primer_bind     complement(5449..5490)
                     /label=SMASH-13
     primer_bind     5471..5526
                     /label=SMASH-14
     primer_bind     5506..5564
                     /label=SMASH-15
     primer_bind     5547..5605
                     /label=SMASH-16
     primer_bind     5587..5645
                     /label=SMASH-17
     primer_bind     5629..5687
                     /label=SMASH-18
     primer_bind     5666..5724
                     /label=SMASH-19
     CDS             5702..5734
                     /codon_start=1
                     /label=helicase domain
                     /translation="NSSPPAVTLTH"
     primer_bind     complement(5715..5755)
                     /label=oSCL1431
     primer_bind     complement(5715..5752)
                     /label=oSCL1389
     primer_bind     complement(5735..5772)
                     /label=oYK480_NS3_R
     CDS             5735..5752
                     /codon_start=1
                     /label=StaPL(AI) module
                     /translation="GGSGGS"
     CDS             5735..5752
                     /codon_start=1
                     /label=linker
                     /translation="GGSGGS"
     primer_bind     complement(5735..5752)
                     /label=oSCL1440
     primer_bind     complement(5736..5753)
                     /label=oSCL1436
     CDS             5753..7438
                     /label=Translation 748-6546
     primer_bind     5753..5776
                     /label=oYK480_VPR_F
     primer_bind     complement(5756..5779)
                     /label=oYK464_dCas9_R
     primer_bind     complement(5756..5779)
                     /label=oYK466_dCas9_R
     misc_feature    5756..5776
                     /label=NLS (SV40 short)
                     /label=nonstandard type: signal
     misc_recomb     5814..5834
                     /label=attB1
     primer_bind     5846..5861
                     /label=oYK465_VPR_F
     primer_bind     complement(5866..5881)
                     /label=oSCL1431
     misc_feature    5867..7435
                     /label=VPR
                     /label=nonstandard type: AD
     CDS             5867..6016
                     /label=VP64
     misc_feature    5867..5905
                     
                     /label=nonstandard type: AD
     misc_feature    5906..5944
                     
                     /label=nonstandard type: AD
     misc_feature    5945..5983
                     
                     /label=nonstandard type: AD
     primer_bind     complement(6035..6061)
                     /label=oYK207_dcas9VP64_R
     misc_feature    6041..6061
                     /label=NLS (SV40 short)
                     /label=nonstandard type: signal
     misc_feature    6491..6847
                     /label=RelA (p65) AD
                     /label=nonstandard type: AD
     CDS             6866..7435
                     /label=Rta AD
     CDS             6866..7435
                     /label=Translation 5974-6543
     primer_bind     complement(7411..7464)
                     /label=oYK465_VPR_R
     primer_bind     complement(7439..7464)
                     /label=oYK466_dCas9_R
     primer_bind     complement(7442..7461)
                     /label=oYK207_dcas9VP64_R
     terminator      7538..7765
                     /label=bGH PA
     misc_feature    8433..8510
                     /label=SV40 ORI
     CDS             8652..9443
                     /label=Kan/neoR
     primer_bind     complement(9783..9803)
                     /label=M13-rev
     misc_binding    complement(9809..9831)
                     /label=LacO
     promoter        complement(9836..9865)
                     /label=lac
     CDS             complement(10951..11610)
                     /label=AmpR
     promoter        complement(11850..11878)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 ggatcgaacc cttgccacca tggacaagaa gtactccatt gggctcgcta tcggcacaaa
      961 cagcgtcggc tgggccgtca ttacggacga gtacaaggtg ccgagcaaaa aattcaaagt
     1021 tctgggcaat accgatcgcc acagcataaa gaagaacctc attggcgccc tcctgttcga
     1081 ctccggggag acggccgaag ccacgcggct caaaagaaca gcacggcgca gatatacccg
     1141 cagaaagaat cggatctgct acctgcagga gatctttagt aatgagatgg ctaaggtgga
     1201 tgactctttc ttccataggc tggaggagtc ctttttggtg gaggaggata aaaagcacga
     1261 gcgccaccca atctttggca atatcgtgga cgaggtggcg taccatgaaa agtacccaac
     1321 catatatcat ctgaggaaga agcttgtaga cagtactgat aaggctgact tgcggttgat
     1381 ctatctcgcg ctggcgcata tgatcaaatt tcggggacac ttcctcatcg agggggacct
     1441 gaacccagac aacagcgatg tcgacaaact ctttatccaa ctggttcaga cttacaatca
     1501 gcttttcgaa gagaacccga tcaacgcatc cggagttgac gccaaagcaa tcctgagcgc
     1561 taggctgtcc aaatcccggc ggctcgaaaa cctcatcgca cagctccctg gggagaagaa
     1621 gaacggcctg tttggtaatc ttatcgccct gtcactcggg ctgaccccca actttaaatc
     1681 taacttcgac ctggccgaag atgccaagct tcaactgagc aaagacacct acgatgatga
     1741 tctcgacaat ctgctggccc agatcggcga ccagtacgca gacctttttt tggcggcaaa
     1801 gaacctgtca gacgccattc tgctgagtga tattctgcga gtgaacacgg agatcaccaa
     1861 agctccgctg agcgctagta tgatcaagcg ctatgatgag caccaccaag acttgacttt
     1921 gctgaaggcc cttgtcagac agcaactgcc tgagaagtac aaggaaattt tcttcgatca
     1981 gtctaaaaat ggctacgccg gatacattga cggcggagca agccaggagg aattttacaa
     2041 atttattaag cccatcttgg aaaaaatgga cggcaccgag gagctgctgg taaagcttaa
     2101 cagagaagat ctgttgcgca aacagcgcac tttcgacaat ggaagcatcc cccaccagat
     2161 tcacctgggc gaactgcacg ctatcctcag gcggcaagag gatttctacc cctttttgaa
     2221 agataacagg gaaaagattg agaaaatcct cacatttcgg ataccctact atgtaggccc
     2281 cctcgcccgg ggaaattcca gattcgcgtg gatgactcgc aaatcagaag agaccatcac
     2341 tccctggaac ttcgaggaag tcgtggataa gggggcctct gcccagtcct tcatcgaaag
     2401 gatgactaac tttgataaaa atctgcctaa cgaaaaggtg cttcctaaac actctctgct
     2461 gtacgagtac ttcacagttt ataacgagct caccaaggtc aaatacgtca cagaagggat
     2521 gagaaagcca gcattcctgt ctggagagca gaagaaagct atcgtggacc tcctcttcaa
     2581 gacgaaccgg aaagttaccg tgaaacagct caaagaagac tatttcaaaa agattgaatg
     2641 tttcgactct gttgaaatca gcggagtgga ggatcgcttc aacgcatccc tgggaacgta
     2701 tcacgatctc ctgaaaatca ttaaagacaa ggacttcctg gacaatgagg agaacgagga
     2761 cattcttgag gacattgtcc tcacccttac gttgtttgaa gatagggaga tgattgaaga
     2821 acgcttgaaa acttacgctc atctcttcga cgacaaagtc atgaaacagc tcaagaggcg
     2881 ccgatataca ggatgggggc ggctgtcaag aaaactgatc aatgggatcc gagacaagca
     2941 gagtggaaag acaatcctgg attttcttaa gtccgatgga tttgccaacc ggaacttcat
     3001 gcagttgatc catgatgact ctctcacctt taaggaggac atccagaaag cacaagtttc
     3061 tggccagggg gacagtcttc acgagcacat cgctaatctt gcaggtagcc cagctatcaa
     3121 aaagggaata ctgcagaccg ttaaggtcgt ggatgaactc gtcaaagtaa tgggaaggca
     3181 taagcccgag aatatcgtta tcgagatggc ccgagagaac caaactaccc agaagggaca
     3241 gaagaacagt agggaaagga tgaagaggat tgaagagggt ataaaagaac tggggtccca
     3301 aatccttaag gaacacccag ttgaaaacac ccagcttcag aatgagaagc tctacctgta
     3361 ctacctgcag aacggcaggg acatgtacgt ggatcaggaa ctggacatca atcggctctc
     3421 cgactacgac gtggctgcta tcgtgcccca gtcttttctc aaagatgatt ctattgataa
     3481 taaagtgttg acaagatccg ataaagctag agggaagagt gataacgtcc cctcagaaga
     3541 agttgtcaag aaaatgaaaa attattggcg gcagctgctg aactccaaac tgatcacaca
     3601 acggaagttc gataatctga ctaaggctga acgaggtggc ctgtctgagt tggataaagc
     3661 cggcttcatc aaaaggcagc ttgttgagac acgccagatc accaagcacg tggcccaaat
     3721 tctcgattca cgcatgaaca ccaagtacga tgaaaatgac aaactgattc gagaggtgaa
     3781 agttattact ctgaagtcta agctggtctc agatttcaga aaggactttc agttttataa
     3841 ggtgagagag atcaacaatt accaccatgc gcatgatgcc tacctgaatg cagtggtagg
     3901 cactgcactt atcaaaaaat atcccaagct tgaatctgaa tttgtttacg gagactataa
     3961 agtgtacgat gttaggaaaa tgatcgcaaa gtctgagcag gaaataggca aggccaccgc
     4021 taagtacttc ttttacagca atattatgaa ttttttcaag accgagatta cactggccaa
     4081 tggagagatt cggaagcgac cacttatcga aacaaacgga gaaacaggag aaatcgtgtg
     4141 ggacaagggt agggatttcg cgacagtccg gaaggtcctg tccatgccgc aggtgaacat
     4201 cgttaaaaag accgaagtac agaccggagg cttctccaag gaaagtatcc tcccgaaaag
     4261 gaacagcgac aagctgatcg cacgcaaaaa agattgggac cccaagaaat acggcggatt
     4321 cgattctcct acagtcgctt acagtgtact ggttgtggcc aaagtggaga aagggaagtc
     4381 taaaaaactc aaaagcgtca aggaactgct gggcatcaca atcatggagc gatcaagctt
     4441 cgaaaaaaac cccatcgact ttctcgaggc gaaaggatat aaagaggtca aaaaagacct
     4501 catcattaag cttcccaagt actctctctt tgagcttgaa aacggccgga aacgaatgct
     4561 cgctagtgcg ggcgagctgc agaaaggtaa cgagctggca ctgccctcta aatacgttaa
     4621 tttcttgtat ctggccagcc actatgaaaa gctcaaaggg tctcccgaag ataatgagca
     4681 gaagcagctg ttcgtggaac aacacaaaca ctaccttgat gagatcatcg agcaaataag
     4741 cgaattctcc aaaagagtga tcctcgccga cgctaacctc gataaggtgc tttctgctta
     4801 caataagcac agggataagc ccatcaggga gcaggcagaa aacattatcc acttgtttac
     4861 tctgaccaac ttgggcgcgc ctgcagcctt caagtacttc gacaccacca tagacagaaa
     4921 gcggtacacc tctacaaagg aggtcctgga cgccacactg attcatcagt caattacggg
     4981 gctctatgaa acaagaatcg acctctctca gctcggtgga gacagcaggg ctgctagtgg
     5041 taccgatgag atggaagagt gctctcagca cggcggatct ggaggttcta caggctgcgt
     5101 ggtcatagtg ggcaggatcg tcttgtccgg atctggcact agtgcgccca tcacggcgta
     5161 cgcccagcag acgagaggcc tcctagggtg tataatcacc agcctgactg gccgggacaa
     5221 aaaccaagtg gagggtgagg tccagatcgt gtcaactgct acccaaacct tcctggcaac
     5281 gtgcatcaat ggggtatgct gggcagtcta ccacggggcc ggaacgagga ccatcgcatc
     5341 acccaagggt cctgtcatcc agatgtatac caatgtggac caagaccttg tgggctggcc
     5401 cgctcctcaa ggttcccgct cattgacacc ctgtacctgc ggctcctcgg acctttacct
     5461 ggtcacgagg cacgccgatg tcattcccgt gcgccggcga ggtgatagca ggagtagcct
     5521 gctttcgccc cggcccattt cctacttgaa aggctcctct gggggtccgc tgttgtgccc
     5581 cgcgggacac gccgtgggcc tattcagggc cgcggtgtgc acccgtggag tggctaaagc
     5641 ggtggacttt atccctgtgg agaacctaga gacaaccatg agatccccgg tgttcacgga
     5701 caactcctct ccaccagcag tcaccctgac gcacgggggt tcaggcggta gtgaccccaa
     5761 gaagaagagg aaggtgtcgc cagggatccg tcgacttgac gcgttgatat caacaagttt
     5821 gtacaaaaaa gcaggctaca aagaggccag cggttccgga cgggctgacg cattggacga
     5881 ttttgatctg gatatgctgg gaagtgacgc cctcgatgat tttgaccttg acatgcttgg
     5941 ttcggatgcc cttgatgact ttgacctcga catgctcggc agtgacgccc ttgatgattt
     6001 cgacctggac atgctgatta actctagaag ttccggatct ccgaaaaaga aacgcaaagt
     6061 tggtagccag tacctgcccg acaccgacga ccggcaccgg atcgaggaaa agcggaagcg
     6121 gacctacgag acattcaaga gcatcatgaa gaagtccccc ttcagcggcc ccaccgaccc
     6181 tagacctcca cctagaagaa tcgccgtgcc cagcagatcc agcgccagcg tgccaaaacc
     6241 tgccccccag ccttacccct tcaccagcag cctgagcacc atcaactacg acgagttccc
     6301 taccatggtg ttccccagcg gccagatctc tcaggcctct gctctggctc cagcccctcc
     6361 tcaggtgctg cctcaggctc ctgctcctgc accagctcca gccatggtgt ctgcactggc
     6421 tcaggcacca gcacccgtgc ctgtgctggc tcctggacct ccacaggctg tggctccacc
     6481 agcccctaaa cctacacagg ccggcgaggg cacactgtct gaagctctgc tgcagctgca
     6541 gttcgacgac gaggatctgg gagccctgct gggaaacagc accgatcctg ccgtgttcac
     6601 cgacctggcc agcgtggaca acagcgagtt ccagcagctg ctgaaccagg gcatccctgt
     6661 ggcccctcac accaccgagc ccatgctgat ggaatacccc gaggccatca cccggctcgt
     6721 gacaggcgct cagaggcctc ctgatccagc tcctgcccct ctgggagcac caggcctgcc
     6781 taatggactg ctgtctggcg acgaggactt cagctctatc gccgatatgg atttctcagc
     6841 cttgctgggc tctggcagcg gcagccggga ttccagggaa gggatgtttt tgccgaagcc
     6901 tgaggccggc tccgctatta gtgacgtgtt tgagggccgc gaggtgtgcc agccaaaacg
     6961 aatccggcca tttcatcctc caggaagtcc atgggccaac cgcccactcc ccgccagcct
     7021 cgcaccaaca ccaaccggtc cagtacatga gccagtcggg tcactgaccc cggcaccagt
     7081 ccctcagcca ctggatccag cgcccgcagt gactcccgag gccagtcacc tgttggagga
     7141 tcccgatgaa gagacgagcc aggctgtcaa agcccttcgg gagatggccg atactgtgat
     7201 tccccagaag gaagaggctg caatctgtgg ccaaatggac ctttcccatc cgcccccaag
     7261 gggccatctg gatgagctga caaccacact tgagtccatg accgaggatc tgaacctgga
     7321 ctcacccctg accccggaat tgaacgagat tctggatacc ttcctgaacg acgagtgcct
     7381 cttgcatgcc atgcatatca gcacaggact gtccatcttc gacacatctc tgttttgata
     7441 aggatccact agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgctc
     7501 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     7561 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     7621 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     7681 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     7741 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     7801 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7861 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     7921 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     7981 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     8041 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     8101 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     8161 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     8221 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     8281 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     8341 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     8401 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     8461 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     8521 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     8581 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     8641 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     8701 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     8761 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     8821 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     8881 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     8941 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     9001 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     9061 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     9121 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     9181 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     9241 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     9301 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     9361 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     9421 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     9481 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     9541 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     9601 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     9661 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     9721 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     9781 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     9841 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     9901 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     9961 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
    10021 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    10081 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    10141 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    10201 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    10261 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    10321 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    10381 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    10441 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    10501 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    10561 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    10621 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    10681 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10741 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    10801 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    10861 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    10921 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    10981 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    11041 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    11101 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    11161 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    11221 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    11281 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    11341 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    11401 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    11461 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    11521 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    11581 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    11641 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    11701 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    11761 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    11821 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    11881 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    11941 cgtc
//