| 质粒名称: | pYK565 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000826 |
| 产品用途: | Mammalian PhCMV-driven constitutive expression vector for 3xFLAG-tagged ZFPCDKN1C125-NLS-4xANR (PhCMV-ZFPCDKN1C125-3xFLAG-NLS-4xANR-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | 3xFLAG-ZFPCDKN1C125-NLS-4xANR |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK565
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK565载体质粒基本信息
pYK565质粒图谱载体图谱和pYK565载体序列质粒序列多克隆位点信息
pYK565质粒载体简介
pYK565质粒序列载体序列
LOCUS pYK565 6804 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6804)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6804
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
primer_bind complement(881..900)
/label=oYK236_Nhe_R
CDS 901..1611
/codon_start=1
/label=ZF_CDKN1C(125)
/translation="MFACDICGRKFARKYHLSRHTRIHTGEKPFACDICGRKFADRSTL
RRHTKIHTQRPQIPPKPFACDICGRKFARKDHLRNHTRIHTGEKPFACDICGRKFAFPY
LLRRHTKIHTQRPQIPPKPFACDICGRKFALKHHLLRHTRIHTGEKPFACDICGRKFAE
RSKLRRHTKIHTQRPQIPPKPFACDICGRKFALKQTLQVHTRIHTGEKPFACDICGRKF
ADRSTLRRHTKIHSR"
primer_bind 903..925
/label=oYK652_ZF125_F
primer_bind complement(1612..1635)
/label=oYK563_ZF_R
primer_bind 1616..1639
/label=oYK563_Flag_F
CDS 1621..1686
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
primer_bind complement(1661..1724)
/label=oYK502_ZF_R
CDS 1693..1713
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind 1715..1736
/label=oSCL1192
primer_bind 1715..1736
/label=oSCL1129
primer_bind 1715..1734
/label=oLZ147 NS3a F
primer_bind 1715..1733
/label=oYF0033
misc_feature 1717..1752
/label=linker
primer_bind 1717..1746
/label=oSCL388
misc_feature 1717..1737
/label=linker
primer_bind 1717..1736
/label=oLZ048 NSP3-F
primer_bind 1717..1732
/label=oYK28-mCherry-NS3a-F
primer_bind complement(1721..1736)
/label=oYK520_GS_R
primer_bind complement(1727..1752)
/label=oSCL1119
primer_bind complement(1727..1746)
/label=oSCL075
primer_bind complement(1730..1746)
/label=oYK472_NLS_R
primer_bind complement(1731..1766)
/label=oYK92_L7Ae_R
primer_bind complement(1735..1753)
/label=oYK75_NLS_mcp_R
primer_bind complement(1741..1795)
/label=oSCL388
CDS 1747..1821
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(1747..1792)
/label=oYK215_Mcp_B_R
primer_bind complement(1747..1766)
/label=oYK195_ANR_R
primer_bind 1747..1761
/label=oYK82_LinkANR_F
primer_bind complement(1748..1767)
/label=test2
primer_bind 1773..1792
/label=oYK215_ANR1rep_F
primer_bind complement(1773..1792)
/label=oYK215_ANR1rep_R
primer_bind 1802..1821
/label=test1
primer_bind complement(1805..1821)
/label=TEST3
CDS 1822..1884
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1822..1837
/label=Primer 4
primer_bind complement(1861..1904)
/label=oYK195_ANR_R
primer_bind complement(1866..1884)
/label=oYK82_LinkANR_R
primer_bind 1869..1883
/label=Primer 3
primer_bind complement(1880..1930)
/label=oYK215_Mcp_B_R
CDS 1885..1959
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(1885..1904)
/label=oYK92_L7Ae_R
primer_bind 1885..1899
/label=oYK82_LinkANR_F
primer_bind complement(1886..1905)
/label=test2
primer_bind 1911..1930
/label=oYK215_ANR1rep_F
primer_bind complement(1911..1930)
/label=oYK215_ANR1rep_R
primer_bind 1940..1959
/label=test1
primer_bind complement(1943..1959)
/label=TEST3
CDS 1960..2022
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1960..1975
/label=Primer 4
primer_bind complement(1999..2042)
/label=oYK195_ANR_R
primer_bind complement(2004..2022)
/label=oYK82_LinkANR_R
primer_bind 2007..2021
/label=Primer 3
primer_bind complement(2018..2068)
/label=oYK215_Mcp_B_R
CDS 2023..2097
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(2023..2042)
/label=oYK92_L7Ae_R
primer_bind 2023..2037
/label=oYK82_LinkANR_F
primer_bind complement(2024..2043)
/label=test2
primer_bind 2049..2068
/label=oYK215_ANR1rep_F
primer_bind complement(2049..2068)
/label=oYK215_ANR1rep_R
primer_bind 2078..2097
/label=test1
primer_bind complement(2081..2097)
/label=TEST3
CDS 2098..2160
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 2098..2113
/label=Primer 4
primer_bind complement(2137..2180)
/label=oYK195_ANR_R
primer_bind complement(2142..2160)
/label=oYK82_LinkANR_R
primer_bind 2145..2159
/label=Primer 3
primer_bind complement(2156..2206)
/label=oYK215_Mcp_B_R
CDS 2161..2235
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(2161..2180)
/label=oYK92_L7Ae_R
primer_bind 2161..2175
/label=oYK82_LinkANR_F
primer_bind complement(2162..2181)
/label=test2
primer_bind 2187..2206
/label=oYK215_ANR1rep_F
primer_bind complement(2187..2206)
/label=oYK215_ANR1rep_R
primer_bind 2216..2235
/label=test1
primer_bind complement(2219..2235)
/label=TEST3
CDS 2236..2298
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 2236..2251
/label=Primer 4
primer_bind complement(2275..2298)
/label=oYK195_ANR_R
primer_bind complement(2280..2321)
/label=oYK82_LinkANR_R
primer_bind 2283..2297
/label=Primer 3
primer_bind complement(2299..2324)
/label=oYK472_NLS_R
primer_bind complement(2302..2324)
/label=oYK57-MCP-mCherry-R
terminator 2398..2625
/label=bGH PA
misc_feature 2671..3099
/label=f1 origin
misc_feature 3104..3447
/label=SV40 early promoter and origin
misc_feature 3293..3370
/label=SV40 ORI
CDS 3512..4303
/label=Kan/neoR
misc_feature 4477..4607
/label=SV40 early polyadenylation signal
primer_bind complement(4643..4663)
/label=M13-rev
misc_binding complement(4669..4691)
/label=LacO
promoter complement(4696..4725)
/label=lac
misc_feature complement(4990..5663)
/label=pUC origin
CDS complement(5811..6470)
/label=AmpR
promoter complement(6710..6738)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 atgttcgcat gtgacatctg tggtcggaag ttcgccagaa agtaccatct gtccaggcat
961 accagaatcc ataccggcga gaaaccattt gcttgtgaca tttgtggccg gaaattcgcc
1021 gatagaagca cactgagacg gcatacaaag atccacacac agcggcctca gattccacct
1081 aagcctttcg cctgtgacat ctgtggaagg aagttcgctc ggaaagacca tcttcggaac
1141 catacacgga tacacactgg tgagaagcct ttcgcatgtg acatctgcgg tagaaagttc
1201 gcattccctt acctgctgag gcgccatacc aagatccata ctcagcggcc acaaattcca
1261 ccaaagccat tcgcttgtga tatctgtgga cggaaattcg cactgaagca ccatctgttg
1321 cggcataccc ggattcacac aggcgagaag ccattcgcct gcgacatttg cggccggaag
1381 ttcgctgaga gatccaaact gcggaggcac actaagattc acacacagcg gccacagata
1441 ccacctaaac cattcgcatg cgacatctgt ggcagaaagt tcgccttgaa gcagaccttg
1501 caggtccata cacgcattca cacaggtgag aaacctttcg cttgcgatat ctgcggtcgc
1561 aagttcgccg acagatccac cttgagacgc catactaaga tccattccag gacctgcaga
1621 gactacaaag accatgacgg cgactataaa gatcacgaca tcgattacaa agacgacgac
1681 gacaagatgg ctccaaagaa gaagagaaag gtgggtgcca gcggaagtgg cggaggaggc
1741 gacgtcggct ccggaacggg cagcgggacc ggaagtggca ccggaaccac ttccggaacc
1801 ggtactggcg ggagtaccgg cggtgagcta gacgaactcg tgtacctgtt ggatggcccc
1861 ggttacgacc caatccacag cgatggctcc ggaacgggca gcgggaccgg aagtggcacc
1921 ggaaccactt ccggaaccgg tactggcggg agtaccggcg gtgagctaga cgaactcgtg
1981 tacctgttgg atggccccgg ttacgaccca atccacagcg atggctccgg aacgggcagc
2041 gggaccggaa gtggcaccgg aaccacttcc ggaaccggta ctggcgggag taccggcggt
2101 gagctagacg aactcgtgta cctgttggat ggccccggtt acgacccaat ccacagcgat
2161 ggctccggaa cgggcagcgg gaccggaagt ggcaccggaa ccacttccgg aaccggtact
2221 ggcgggagta ccggcggtga gctagacgaa ctcgtgtacc tgttggatgg ccccggttac
2281 gacccaatcc acagcgatta aggatccact agtccagtgt ggtggaattc tgcagatatc
2341 cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga
2401 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
2461 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
2521 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
2581 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
2641 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
2701 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
2761 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
2821 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
2881 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
2941 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
3001 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
3061 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
3121 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
3181 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
3241 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
3301 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
3361 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
3421 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
3481 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
3541 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
3601 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
3661 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
3721 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
3781 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
3841 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
3901 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
3961 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
4021 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
4081 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
4141 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
4201 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
4261 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
4321 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
4381 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
4441 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
4501 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
4561 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
4621 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
4681 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
4741 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
4801 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
4861 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
4921 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
4981 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
5041 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
5101 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
5161 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
5221 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
5281 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
5341 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
5401 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
5461 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
5521 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
5581 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
5641 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
5701 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
5761 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
5821 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
5881 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
5941 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
6001 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
6061 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
6121 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
6181 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
6241 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
6301 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
6361 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
6421 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
6481 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
6541 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
6601 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
6661 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
6721 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
6781 ccccgaaaag tgccacctga cgtc
//
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