pPR3S-C-bk1E

LOCUS       pPR3S-C-bk1E              6668 bp DNA     circular SYN 30-OCT-2025
DEFINITION  pPR3S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 30, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        991..1009
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1030..1734
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     primer_bind     1558..1576
                     /label=ADH1F
     CDS             1745..1804
                     /codon_start=1
                     /label=SUC2
                     /translation="MMLLQAFLFLLAGFAAKISA"
     CDS             1900..1926
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1936..2022
                     /codon_start=1
                     /label=NubG
                     /translation="MQIFVKTLTGKTGTLEVESSDTIDNVKSK"
     terminator      2066..2313
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(2332..2350)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2360..2376)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2517..2972
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(3075..3749)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(3750..4031)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     protein_bind    4622..4669
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        5534..5638
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5639..6499
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
       61 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      121 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      661 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      721 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
      781 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
      841 ttcccgactg gaaagccgcg gatgattacg ccaagcgcgc aattaaccaa tacgcaaacc
      901 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
      961 gaaagccgcg gatgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     1021 ggagctcata tccttttgtt gtttccgggt gtacaatatg gacttcctct tttctggcaa
     1081 ccaaacccat acatcgggat tcctataata ccttcgttgg tctccctaac atgtaggtgg
     1141 cggaggggag atatacaata gaacagatac cagacaagac ataatgggct aaacaagact
     1201 acaccaatta cactgcctca ttgatggtgg tacataacga actaatactg tagccctaga
     1261 cttgatagcc atcatcatat cgaagtttca ctaccctttt tccatttgcc atctattgaa
     1321 gtaataatag gcgcatgcaa cttcttttct ttttttttct tttctctctc ccccgttgtt
     1381 gtctcaccat atccgcaatg acaaaaaaat gatggaagac actaaaggaa aaaattaacg
     1441 acaaagacag caccaacaga tgtcgttgtt ccagagctga tgaggggtat ctcgaagcac
     1501 acgaaacttt ttccttcctt cattcacgca cactactctc taatgagcaa cggtatacgg
     1561 ccttccttcc agttacttga atttgaaata aaaaaaagtt tgctgtcttg ctatcaagta
     1621 taaatagacc tgcaattatt aatcttttgt ttcctcgtca ttgttctcgt tccctttctt
     1681 ccttgtttct ttttctgcac aatatttcaa gctataccaa gcatacaatc aactcacaag
     1741 ctatatgatg cttttgcaag ctttcctttt ccttttggct ggttttgcag ccaaaatatc
     1801 tgcaatggcc actagtgcca ggtcgactct cgaggatccg aattcctgca gggtacccgg
     1861 tatcgattta aaactagtga tatcaagctt atcgatatgt acccatacga tgttccagat
     1921 tacgctccta cacccatgca gattttcgtc aagactttga ccggtaaaac cggaacattg
     1981 gaagttgaat cttccgatac catcgacaac gttaagtcga aaattcaaga caaggaagga
     2041 atccctggtg gatgtcgacc tcgagtcatg taattagtta tgtcacgctt acattcacgc
     2101 cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
     2161 cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt
     2221 cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag
     2281 aaggttttgg gacgctcgaa ggctttaatt tgcggccggt acccaattcg ccctatagtg
     2341 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     2401 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     2461 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc
     2521 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     2581 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     2641 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     2701 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     2761 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     2821 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     2881 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     2941 gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat
     3001 ctgtgcggta tttcacaccg catagatcgg caagtgcaca aacaatactt aaataaatac
     3061 tactcagtaa taacctattt cttagcattt ttgacgaaat ttgctatttt gttagagtct
     3121 tttacaccat ttgtctccac acctccgctt acatcaacac caataacgcc atttaatcta
     3181 agcgcatcac caacattttc tggcgtcagt ccaccagcta acataaaatg taagctttcg
     3241 gggctctctt gccttccaac ccagtcagaa atcgagttcc aatccaaaag ttcacctgtc
     3301 ccacctgctt ctgaatcaaa caagggaata aacgaatgag gtttctgtga agctgcactg
     3361 agtagtatgt tgcagtcttt tggaaatacg agtcttttaa taactggcaa accgaggaac
     3421 tcttggtatt cttgccacga ctcatctcca tgcagttgga cgatatcaat gccgtaatca
     3481 ttgaccagag ccaaaacatc ctccttaggt tgattacgaa acacgccaac caagtatttc
     3541 ggagtgcctg aactattttt atatgctttt acaagacttg aaattttcct tgcaataacc
     3601 gggtcaattg ttctctttct attgggcaca catataatac ccagcaagtc agcatcggaa
     3661 tctagagcac attctgcggc ctctgtgctc tgcaagccgc aaactttcac caatggacca
     3721 gaactacctg tgaaattaat aacagacata ctccaagctg cctttgtgtg cttaatcacg
     3781 tatactcacg tgctcaatag tcaccaatgc cctccctctt ggccctctcc ttttcttttt
     3841 tcgaccgaat taattcttaa tcggcaaaaa aagaaaagct ccggatcaag attgtacgta
     3901 aggtgacaag ctatttttca ataaagaata tcttccacta ctgccatctg gcgtcataac
     3961 tgcaaagtac acatatatta cgatgctgtt ctattaaatg cttcctatat tatatatata
     4021 gtaatgtcgt tgatctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     4081 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     4141 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     4201 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     4261 atgtcatgat aataatggtt tcttagtatg atccaatatc aaaggaaatg atagcattga
     4321 aggatgagac taatccaatt gaggagtggc agcatataga acagctaaag ggtagtgctg
     4381 aaggaagcat acgatacccc gcatggaatg ggataatatc acaggaggta ctagactacc
     4441 tttcatccta cataaataga cgcatataag tacgcattta agcataaaca cgcactatgc
     4501 cgttcttctc atgtatatat atatacaggc aacacgcaga tataggtgcg acgtgaacag
     4561 tgagctgtat gtgcgcagct cgcgttgcat tttcggaagc gctcgttttc ggaaacgctt
     4621 tgaagttcct attccgaagt tcctattctc tagaaagtat aggaacttca gagcgctttt
     4681 gaaaaccaaa agcgctctga agacgcactt tcaaaaaacc aaaaacgcac cggactgtaa
     4741 cgagctacta aaatattgcg aataccgctt ccacaaacat tgctcaaaag tatctctttg
     4801 ctatatatct ctgtgctata tccctatata acctacccat ccacctttcg ctccttgaac
     4861 ttgcatctaa actcgacctc tacatttttt atgtttatct ctagtattac tctttagaca
     4921 aaaaaattgt agtaagaact attcatagag tgaatcgaaa acaatacgaa aatgtaaaca
     4981 tttcctatac gtagtatata gagacaaaat agaagaaacc gttcataatt ttctgaccaa
     5041 tgaagaatca tcaacgctat cactttctgt tcacaaagta tgcgcaatcc acatcggtat
     5101 agaatataat cggggatgcc tttatcttga aaaaatgcac ccgcagcttc gctagtaatc
     5161 agtaaacgcg ggaagtggag tcaggctttt tttatggaag agaaaataga caccaaagta
     5221 gccttcttct aaccttaacg gacctacagt gcaaaaagtt atcaagagac tgcattatag
     5281 agcgcacaaa ggagaaaaaa agtaatctaa gatgctttgt tagaaaaata gcgctctcgg
     5341 gatgcatttt tgtagaacaa aaaagaagta tagattcttt gttggtaaaa tagcgctctc
     5401 gcgttgcatt tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa aattagcgct
     5461 ctcgcgttgc atttttgttc tacaaaatga agcacagatg cttcgttcag gtggcacttt
     5521 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5581 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5641 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5701 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5761 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5821 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5881 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5941 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6001 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6061 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6121 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6181 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6241 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6301 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6361 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6421 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6481 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6541 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6601 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6661 aggatctt
//