PAAV-CMV-Zf-GFP

1000 1300 1600 2000 询价

价格：元

联系方式：I47-825O-882O

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PAAV-CMV-Zf-GFP载体质粒基本信息

质粒名称:	PAAV-CMV-Zf-GFP
出品公司:	Wekwikgene
目录编号:	0001191
产品用途:	in vitro
存储实验室:	Yuxuan Guo Lab
载体骨架基本信息
载体名称:	pAAV
表达系统:	哺乳动物表达
启动子:	CMV
拷贝数:	未拷贝
载体抗性:	氨苄霉素
生长温度:	37 ℃
宿主菌:	NEB Stable
插入基因信息
插入基因名称:	GFP
物种来源:	MUS
密码子优化:	未优化
文献引用方法
参考文献部分:	Neural Organoids Protect Engineered Heart Tissues From Glucolipotoxicity by Transferring Versican in a Co-Culture System. Bai B., Li J., Wang Z., Yang Y., He J., Chen G., Zhang Y., Qi Y., Wan Z., Cai L., Wang R., Wang K., Zhao D., Zhang J., Huang W., Xu X.R., Sun M., Han X., Liu Y., Zhang D., Zhu W., Liu J., Guo Y. Cell Prolif. 0, e70070 (2025)

PAAV-CMV-Zf-GFP质粒图谱载体图谱和PAAV-CMV-Zf-GFP载体序列质粒序列多克隆位点信息

PAAV-CMV-Zf-GFP载体图谱

PAAV-CMV-Zf-GFP质粒载体简介

PAAV-CMV-Zf-GFP质粒序列载体序列

LOCUS       PAAV-CMV-Zf-GFP     circular     21-JUN-2024
DEFINITION  .
COMMENT     ^^
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /label="5' ITR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    29..34
                     /label="XmaI"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    40..45
                     /label="XmaI"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    179..201
                     /label="overlap"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    202..225
                     /label="primer-F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     enhancer        217..596
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    573..592
                     /label="overlap"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          573..617
                     /label="CMV-DMX-clone-F"
                     /note="sequence: gtattagtcatcgctattaccatggtgatgcggttttggcagtac"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        597..800
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     751..771
                     /label="CMV-F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    753..757
                     /label="CAAT box"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    786..792
                     /label="TATA box -23~-29"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    801..814
                     /label="overlap"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    811..827
                     /label="overlap"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          811..848
                     /label="Zfr-clone-F1"
                     /note="sequence: cggccgcactactccgcACTGACCAACAGCGTGAAGAC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    812..827
                     /label="overlap"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    815..815
                     /label="TSS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    828..848
                     /label="overlap"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     exon            828..866
                     /label="Zfr-201 Exon 19"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    828..866
                     /label="upstream"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     gene            828..2061
                     /label="Zfr minigene"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          840..863
                     /label="Zfr-rtpcr-F"
                     /note="sequence: CGTGAAGACATTACATCCAGTGCA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    867..1116
                     /label="keep"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1098..1116
                     /label="overlap"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1098..1139)
                     /label="Zfr-clone-R1"
                     /note="sequence: GGTTTATATGTGCCACAAGTATAACTTTGGTGGGCCTTCGTG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1117..1139
                     /label="overlap"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          1117..1165
                     /label="Zfr-clone-F2"
                     /note="sequence: TATACTTGTGGCACATATAAACCTTTGGAATCAAGGGACCTGATACTGG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1117..1366
                     /label="keep"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1140..1165
                     /label="overlap"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1367..1450
                     /label="PSEX"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             1370..1450
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="KGLHIKNIV*TL*GQRSVLISPD*KL*"
     primer          1416..1440
                     /label="Zfr-qpcr-F"
                     /note="sequence: GAAGTGTGCTCATTTCTCCCGATTA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             1447..1450
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="M"
     misc_feature    1451..2019
                     /label="keep"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     exon            2020..2061
                     /label="Zfr-201 Exon 20"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2020..2061
                     /label="downstream"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             2022..2060
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="CIETPCIPSDTQS"
     misc_feature    2035..2061
                     /label="overlap"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(2035..2079)
                     /label="Zfr-clone-R2"
                     /note="sequence: CCTTGCTCACAAGCTTGcAACTTTGTGTATCTGACGGAATGCAAG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             2046..2084
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="SDTQSCKLVSKGE"
     misc_feature    2062..2062
                     /label="insertion"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2063..2079
                     /label="overlap"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2063..2080
                     /label="overlap"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             2070..2783
                     /label="GFP"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             2070..2783
                     /label="Translation 2070-2783"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer          complement(2131..2151)
                     /label="DM3-GFP-R"
                     /note="sequence: TGAACTTGTGGCCGTTTACGT"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(2367..2390)
                     /label="GFP-qPCR-R"
                     /note="sequence: CTTGTAGTTGCCGTCGTCCTTGAA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2776..2781
                     /label="BsrGI"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2798..2803
                     /label="KpnI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2815..2820
                     /label="XmaI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     terminator      2858..2984
                     /label="PolyA"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3073..3202
                     /label="3' ITR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3158..3163
                     /label="XmaI"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3169..3174
                     /label="XmaI"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     rep_origin      3379..3834
                     /label="f1 origin"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3860..3964
                     /label="Amp Promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3965..4822
                     /label="Amp resistent"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     rep_origin      4996..5584
                     /label="origin"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 CTGCGCGCTC GCTCGCTCAC TGAGGCCGCC CGGGCAAAGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT TGTAGTTAAT GATTAACCCG CCATGCTACT TATCTACCAG GGTAATGGGG
      181 ATCCTCTAGA ACTATAGCTA Gccagatata cgcgttgaca ttgattattg actagttatt
      241 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      301 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      361 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      421 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      481 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      541 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      601 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      661 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      721 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      781 aggtctatat aagcagagct CCTCGAGAAG cggccgcact actccgcACT GACCAACAGC
      841 GTGAAGACAT TACATCCAGT GCACAGGTAA TTGAATTCTT GTTGGATTAC TGTCTCTAAG
      901 GCCTGGGAGT TTTATAATTG TTTCTTTATG TGTAATAATA ACCGTACTAG TCATTGTGAT
      961 ACTTATTTGG GAAGTAAGCC TTTAAAGAGG TTATTAAAAA GATTTACTGA TTCCCACACC
     1021 GGAAGTTGCT CTTCTAGCCG TGAAAGAAGG ACGGCGTGGT CTTCAGTTTG CTATGTCCAC
     1081 TAACAATATG TCCGACCCAC GAAGGCCCAC CAAAGTTATA CTTGTGGCAC ATATAAACCT
     1141 TTGGAATCAA GGGACCTGAT ACTGGATTAT TAAATATTTA AGAGAATTTA TATTTAATAA
     1201 ACTTGATACT TCAGTGAGAA AGGACAAATC ATCAGTAGAA TGGTTTTGAG ATAGCTGATG
     1261 ATTCTATTAA AAGTAGATAT GTGTGTGGGT TAAATGACTT AAGCATACAT CAAAAGACTG
     1321 AGAAAGGCTC TTAACATTAA AAGATGGGCC tttttttttC TTTTAGGTTA AAGGATTACA
     1381 TATTAAAAAT ATTGTATAGA CTCTTTAAGG CCAAAGAAGT GTGCTCATTT CTCCCGATTA
     1441 AAAGTTATGA GTAAGAATTG GGTATTTTGC CTTTTGGGGG TGGGAGAttt gaaacagggt
     1501 ttctccttac agctctggct ttcctggagc agtgaattga gcctggctcc tctgtaggag
     1561 taacgagttg ctttaaccac tgacatcttt ccaccaccac caccaccCCT TATGAATAGA
     1621 CTAAATTTAT TTAAAGATTG ATATTTTCTA GCTTAATTAT TTGGAAAGCA AATAAGCACA
     1681 CAGAAAAGCC TCCTCCCCaa aaaaatataa ataaaaaaGT CTATCAGGAC ATAGTATATG
     1741 TCCCTTCTGA TTAATCATTG GTGGTGAAAC AACCCATTCA GGGTGTCTCT TCCATCACAG
     1801 TGGAtttttt tCATATTGTT ACATGGAGAA GTTAACTTAG TAAGAATTGA TGTGCTTCTG
     1861 TGCCAGTGAG ATAGCTCTGC AGGTAAAAGG GAGACTGACC CCCAACTTGT ACTCTGGCCG
     1921 TGGCAGTCAT GTGCACACAA GTAAACTGAA TAAATATAAA GAGCAGATTT TAGAGTGTTT
     1981 TGGCTTTAAA TTAATTCTAT ATTCTAACTC TATTTGCAGT TTGCATTGAG ACTCCTTGCA
     2041 TTCCGTCAGA TACACAAAGT TgCAAGCTTG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG
     2101 TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG
     2161 GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG
     2221 GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT
     2281 TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG
     2341 GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG
     2401 AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA
     2461 AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT
     2521 ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA
     2581 TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG
     2641 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC
     2701 CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC
     2761 TCGGCATGGA CGAGCTGTAC AAGTAATAAG CTCGCGTGGT ACCTCTAGAG TCGACCCGGG
     2821 CGGCCTCGAG GACGGGGTGA ACTACGCCTG AGGATCCGAT CTTTTTCCCT CTGCCAAAAA
     2881 TTATGGGGAC ATCATGAAGC CCCTTGAGCA TCTGACTTCT GGCTAATAAA GGAAATTTAT
     2941 TTTCATTGCA ATAGTGTGTT GGAATTTTTT GTGTCTCTCA CTCGGAAGCA ATTCGTTGAT
     3001 CTGAATTTCG ACCACCCATA ATACCCATTA CCCTGGTAGA TAAGTAGCAT GGCGGGTTAA
     3061 TCATTAACTA CAAGGAACCC CTAGTGATGG AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT
     3121 CGCTCACTGA GGCCGGGCGA CCAAAGGTCG CCCGACGCCC GGGCTTTGCC CGGGCGGCCT
     3181 CAGTGAGCGA GCGAGCGCGC AGCCTTAATT AACCTAATTC ACTGGCCGTC GTTTTACAAC
     3241 GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT
     3301 TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA
     3361 GCCTGAATGG CGAATGGGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG
     3421 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT
     3481 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC
     3541 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG
     3601 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT
     3661 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG
     3721 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC
     3781 TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA ATTTAGGTGG
     3841 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA
     3901 TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA
     3961 GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT
     4021 TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG
     4081 TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG
     4141 CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT
     4201 ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA
     4261 CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA
     4321 ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC
     4381 GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG
     4441 CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC
     4501 GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT
     4561 AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT
     4621 GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG
     4681 GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT
     4741 CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG
     4801 TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT
     4861 TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT
     4921 CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA
     4981 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA
     5041 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC
     5101 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA
     5161 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT
     5221 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG
     5281 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG
     5341 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC
     5401 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG
     5461 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT
     5521 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG
     5581 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA
     5641 CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG
     5701 AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC
     5761 GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG
     5821 CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG
     5881 TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG
     5941 TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAG
     6001 ATTTAATTAA GGCCTTAATT AGG
//

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PAAV-CMV-Zf-GFP质粒载体应用举例

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pNeoEGFP	pET-hisPNK
Escherichia coli BGSC ECE144	pIRES-hrGFP-1a
pLenti6.3/V5-GW/EmGFP	sDarken
pET-31b(+)	pCAMBIA1301-CFP
pTRIPZ	pAAV-hSyn-GRAB_CCK1.0
pDE2	pAAVLINK-EF1a-HA-5'-CLTC
pTac-mCherry-TetA	pAAVLINK-FBN1-B
pHisB-Sumo-NeMeHg	pG5luc
pTransketo-TA	pATPase_TA
pHV33	pAAVLINKv2-SCP1-Cre-UDeg3a-3'-YE1-BE4max
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NRXN3-FLAG	pH2B-miRFP670-2
pAAVLINK-EF1a-5'-PCDH15	pAAV-CAG-DIO-TurboID
pPink-HC	pBABE-puro-IRES-EGFP
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ABCC6-FLAG	pAAVLINK-APC-B
pAAV-AscI-CAG-dMito2-Nelle-L3-mCherry	pAAV-CaMKII-GRAB_eCB2.0
pBC-Hygro	pPara-Bgl3082(1)
pGL4.33[luc2P/SRE/Hygro]	pMal-p2X
pSW103	pDONR223
pLVX-IRES-Neo	pVA838
pET-fasG	pTALEN_v2 (NN)
pDG148-Stu	pWF1
pT2-mVenus	pCytERM-iRFP670-N1
pBluescript II SK(+)	pCambia1200
pSilencer 3.1-H1 hygro	pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL4A5-FLA
pMHE3Tc	pCoBlast
pAAV-cFos2-mChilada-iPAK4	pBP47
pAAVLINK-EF1a-HA-5-RALGAPB	pAAVLINKv2-SCP1-Cre-UDeg3a-3'-YEATS2-FLA
pIJ702-87LE	pAAVLINK-EF1a-HA-5'-KDM5A
pAAVLINKv1-pCALM1-Cre-3'-CHD1-FLAG	pmBaoJin-Tubulin
pcDNA3.1-T2A-DsRed2	pGL3-Enhancer
pAAVLINK-3'-PRPF8-FLAG	pPZP100
AAVS1-TF	pENTR-GeneBLAzer
pIRES2-EGFP	pRetroX-Tet-On Advanced
pAAV-CAG-DIO-ElectraON-Kv2.1	pK18mobSacB
pBSE1	pcDNA3.1+C-DYK-P2A
pAAV-hSyn-GRAB_eCBmut	Tet-pLKO-neo
pEF-myc-ER-GFP	pLVX-DsRed-Express2-N1
TOPFlash	pLentG-KOSM
pWPXL	pBADHisB-NMT
Lenti-mEGFP-hsG3BP1 I61A	pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHRF1-FLAG
pEXP2-DEST	pMT/BioEase-DEST
pDisplay-NE2h-IRES-mCherry-CAAX	pLVX-TetOn-TcdB1GTD-EGFP
pLYL63	pET-28a-Ubiquitin
pEarleyGate203	pAAV-CAG-BDFP1.6
pBTB-5	pFC-MEKK
pUBC-mKate2-1POK	pAAV-CAG-eRFP2-P2A-EGFP
Lenti-mEGFP-NXT3	psiRNA-hH1neo G2
pBS42	pMXB10
pcDNA4/His A	pBD-NF-kB
pSF11-wmBaoJin	pBT-2

gag/pol	TRUPATH Triple Gai1
pcDNA3.1-HA-NONO	7TFP CDH1 mutant reporter
pY016 (pcDNA3.1-hLbCpf1)	piRFP
Str-KDEL_ST-SBP-EGFP	pGL3-U6-sgRNA-PGK-puromycin
pLVX-EF1alpha-SARS-CoV-2-nsp11-2xStrep-I	pIVT-dCas9-mCherry
pCDNA3 Flag MKK7B2Jnk1a1	EGFP-p65
pNI6	PX405 Neisseria meningitidis Cas9
pCAGGS_cAMPFIRE-L	DCT.VV.Orf196
pLH-sgRNA1-2XMS2	pRint
pACT1:Cas9-GFP, U6:sgUPRT	pBRC_double_attB_GFP_donor
Lact-C2-GFP	pBsVchCAST
pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast	pCS-kI
pTubb3-MC	Beta-arrestin2 GFP WT
RCASBP(E)(CC#36)	pJYDN3
PB-NEO-GFP-RFP	pCMV-GFP
pcDNA5-FRT/TO-Flag-RNF169	pNH6
pAAV-CamKII-ArchT-GFP	pMXs-Nanog
pRCC-N	Lenti-Hb9-RFP
pHAGE-EFS-PCP-3XGFPnls	Cas9-T6N
pEBB-TB	pEE491
pD649-HAsp-PTGFRN-Fc(DAPA)-AviTag-6xHis	pCE-BiFC-VC155
pWS172	pMOS013: mito-roGFP2-Grx1 glutathione re
Flag-HA-USP53	pcDNA3 APEX2-NES
pCR4-24XMS2SL-stable	pRK5-EGFP-Tau
EGFP-Rab8a-wt	pRE57I-GNAc
pLKO.1 shSV40 neo	PABE-7
pMXspuro-GFP	pNH10
pHSN6I01	T7-VEE-IRES-Puro
pHDM-Tat1b	pREP-8xARE-GFP-SV40-BFP
pcDNA3.4_17G6-1 heavy chain	pcDNA3.1-Flag HBx
pAT03-Apr	pAAV-TRE-ChR2-YFP
pcDNA3/Flag-METTL3	pX460
pAAVscCBPIGpluc	pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-
CJ57 (cg6551 utr)	pAWP78-PVCpnf_pvc13-truncated
pEGFP-C1+SKL	pT/Caggs-NRASV12
IDP427	pREDKI
pOBCol2.3-GFPemd	pLenti-GFP1-9
DsRed-rab11 WT	mATF4
kiCAP-AAV-BI-hTFR1	pCMV-BE3
LentiGuide-TagRFP-2A-BSD	pcg 2.0-BFP-circ-arRNA backbone
pCX-cMyc	CMV-enAsCas12f
pMT-puro	pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destinat
ilux pGEX(-)	pKOV
pLH-sgRNA1-boxB-MS2-PP7	pDO23A
pCAS	pHes1(467)-luc
pGBW-m4046663	pR26 iBFP Dest
AbVec2.1-IGLC2-MscI	p1xNLS-FRB-mCherry-hsp86-BSD
pLenti CMV/TO eGFP Puro (w159-1)	dxCas(3.7)-VPR
hHS1	pX330A_FokI-1x3
pEP4 E02S EN2L	pBAD_sfGFP3TCG4TAG

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