| 质粒名称: | pSL1426 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002440 |
| 产品用途: | AAV2-vector for light-inducible MCP/LIRP-dependent NanoLuc translation (ITR(AAV)-PhCMV-(MS2)8-NanoLuc-pA: PhCMV-LIRP-pA-ITR(AAV)). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pX601 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV, PhCMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | NanoLuc, LIRP |
| 物种来源: | synthetic |
| 密码子优化: | 人源表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
NA
NA
|
pSL1426
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL1426载体质粒基本信息
pSL1426质粒图谱载体图谱和pSL1426载体序列质粒序列多克隆位点信息
pSL1426质粒载体简介
pSL1426质粒序列载体序列
LOCUS pSL1426 6137 bp DNA circular SYN 11-FEB-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6137)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Aug 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6137
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
primer_bind 123..164
/label=oSCL1800
misc_feature 143..725
/label=CMV
primer_bind complement(699..730)
/label=OXB018
primer_bind 706..749
/label=oSCL1791
primer_bind 709..729
/label=oSCL1013
misc_feature 731..732
/label=linker
primer_bind complement(734..748)
/label=Oscl28-2
primer_bind complement(735..748)
/label=8x R
misc_feature 749..761
/label=linker
misc_feature 767..785
/label=MS2 box
misc_feature 791..800
/label=linker
misc_feature 806..824
/label=MS2 box
misc_feature 830..839
/label=linker
misc_feature 830..839
/label=linker
misc_feature 838..868
/label=hairpin
misc_feature 844..862
/label=MS2 box
misc_feature 867..879
/label=linker
misc_feature 878..908
/label=hairpin
misc_feature 884..902
/label=MS2 box
misc_feature 912..917
/label=linker
primer_bind 922..943
/label=oSCL1848
misc_feature 926..941
/label=linker
primer_bind 928..943
/label=oSCL1846
misc_feature 968..980
/label=linker
misc_feature 986..1004
/label=MS2 box
misc_feature 1010..1019
/label=linker
misc_feature 1025..1043
/label=MS2 box
misc_feature 1049..1058
/label=linker
misc_feature 1049..1058
/label=linker
misc_feature 1057..1087
/label=hairpin
misc_feature 1063..1081
/label=MS2 box
misc_feature 1086..1098
/label=linker
misc_feature 1097..1127
/label=hairpin
misc_feature 1103..1121
/label=MS2 box
misc_feature 1131..1136
/label=linker
primer_bind 1141..1198
/label=oSCL1848
misc_feature 1145..1160
/label=linker
primer_bind 1147..1202
/label=oSCL1846
primer_bind 1171..1202
/label=oSCL1108
primer_bind 1175..1202
/label=oSCL1314
primer_bind 1179..1202
/label=oSCL084
misc_feature 1181..1249
/label=Secretory (IgK)
/label=nonstandard type: signal
misc_feature 1271..1780
/label=NLuc (nano Luciferase)
/label=nonstandard type: reporter
misc_feature 1301..1303
/label=cyt-STOP (TGG->TGA)
primer_bind complement(1760..1803)
/label=oSCL1849
primer_bind 1769..1818
/label=oSCL1795
primer_bind complement(1770..1780)
/label=oSCL085
primer_bind complement(1770..1780)
/label=oSCL1109
polyA_signal 1790..1911
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind 1790..1818
/label=OXB007
primer_bind complement(1801..1820)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 1814..1834
/label=PSG5-R(SV40-R)
primer_bind 1855..1874
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(1882..1911)
/label=OXB016
primer_bind complement(1886..1911)
/label=OXB008
primer_bind complement(1895..1917)
/label=oSCL1801
primer_bind complement(1895..1911)
/label=oSCL1174
primer_bind complement(1895..1911)
/label=oSCL1796
misc_feature 1916..2503
/label=CMV
primer_bind 1916..1937
/label=oSCL1800
primer_bind complement(2472..2503)
/label=OXB018
primer_bind 2479..2503
/label=oSCL1791
primer_bind 2482..2502
/label=oSCL1013
primer_bind 2504..2523
/label=T7
primer_bind 2510..2559
/label=oSCL2413
primer_bind 2533..2559
/label=oSCL2381
CDS 2542..2694
/codon_start=1
/label=MS2-N55K
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRSQAYKVT
CSVRQS"
CDS 2695..3105
/codon_start=1
/label=LOV2
/translation="LERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGRNCRF
LQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQYFIG
VQLDGTEHVRDAAEREGVMLIKKTAENIDEAAG"
primer_bind 2699..2741
/label=oSCL2408
primer_bind complement(2699..2718)
/label=oSCL2407
CDS 2719..2721
/codon_start=1
/label=V416I
/translation="V"
primer_bind 3035..3077
/label=oSCL2410
primer_bind complement(3035..3054)
/label=oSCL2409
CDS 3055..3057
/codon_start=1
/label=G528A
/translation="G"
primer_bind 3065..3108
/label=oSCL2412
primer_bind complement(3065..3084)
/label=oSCL2411
CDS 3085..3087
/codon_start=1
/label=N538E
/translation="N"
CDS 3106..3333
/codon_start=1
/label=MS2-N55K
/translation="QKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATN
SDCELIVKAMQGLLKDGNPIPSAIAANSGIY"
primer_bind complement(3314..3356)
/label=oSCL2415
primer_bind complement(3314..3336)
/label=oSCL2383
primer_bind complement(3314..3333)
/label=oSCL2382
primer_bind 3314..3333
/label=oSCL2384
misc_feature 3343..3391
/label=HSV TK poly(A) signal
primer_bind complement(3367..3399)
/label=oSCL1799
repeat_region 3400..3540
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 3400..3529
/label=AAV2 ITR
rep_origin 3615..4070
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3702..3721)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3912..3933
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4087..4106)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4206..4228
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4266..4284)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4352..4456
/gene="bla"
/label=AmpR promoter
CDS 4457..5317
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4675..4694)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5488..6076
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5977..5996
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggcctcta gagttgacat tgattattga ctagttatta atagtaatca
181 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
241 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
301 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
361 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
421 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
481 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
541 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
601 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
661 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
721 agcagagctc cttgaaccgg taagaattgc aacaaacaaa tcaccaccgt gaggatcacc
781 cacggtggtg aaagccttag cctagccgtg aggatcaccc acggtaggca caagaaattc
841 tacccgtgag gatcacccac gggtagaatc acaaagacta cagccgtgag gatcacccac
901 ggctgtagac tcaaacttaa aaaggatatc ttcagcaaac tgatcggaga cgagtccgtc
961 tcaaattgca acaaacaaat caccaccgtg aggatcaccc acggtggtga aagccttagc
1021 ctagccgtga ggatcaccca cggtaggcac aagaaattct acccgtgagg atcacccacg
1081 ggtagaatca caaagactac agccgtgagg atcacccacg gctgtagact caaacttaaa
1141 aaggatatct tcagcaaact gaattcagct tagcgccacc atgactagtg agacagacac
1201 actcctgcta tgggtactgc tgctctgggt tccaggttcc actggtgacg ctagtggtgg
1261 ttctggtatg gtcttcacac tcgaagattt cgttggggac tggcgacaga cagccggcta
1321 caacctggac caagtccttg aacagggagg tgtgtccagt ttgtttcaga atctcggggt
1381 gtccgtaact ccgatccaaa ggattgtcct gagcggtgaa aatgggctga agatcgacat
1441 ccatgtcatc atcccgtatg aaggtctgag cggcgaccaa atgggccaga tcgaaaaaat
1501 ttttaaggtg gtgtaccctg tggatgatca tcactttaag gtgatcctgc actatggcac
1561 actggtaatc gacggggtta cgccgaacat gatcgactat ttcggacggc cgtatgaagg
1621 catcgccgtg ttcgacggca aaaagatcac tgtaacaggg accctgtgga acggcaacaa
1681 aattatcgac gagcgcctga tcaaccccga cggctccctg ctgttccgag taaccatcaa
1741 cggagtgacc ggctggcggc tgtgcgaacg cattctggcg tgactcgagt aagatacatt
1801 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
1861 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt tacgcgttga
1921 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
1981 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
2041 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
2101 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
2161 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
2221 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
2281 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
2341 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
2401 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
2461 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctaatacg actcactata
2521 gggaagcttt aagccaccat ggctagcaac ttcacacagt tcgtgctggt ggacaacggc
2581 ggcaccggcg acgtgaccgt ggcccctagc aacttcgcca acggcatcgc cgagtggatc
2641 agcagcaaca gcagaagcca agcctacaag gtgacctgca gcgtgagaca gagcctggag
2701 agaatcgaga agaacttcgt gatcaccgac cctagactgc ccgacaaccc catcatcttc
2761 gctagcgaca gcttcctgca gctgaccgag tacagcagag aggagatcct gggcagaaac
2821 tgcagattcc tgcaaggccc cgagaccgac agagccaccg tgagaaagat cagagacgcc
2881 atcgacaatc agaccgaggt gaccgtgcag ctgatcaact acaccaagag cggcaagaag
2941 ttctggaacc tgttccacct gcagcccatg agagatcaga agggcgacgt gcagtacttc
3001 atcggcgtgc agctggacgg caccgagcac gtgagagacg ccgccgagag agagggcgtg
3061 atgctgatca agaagaccgc cgagaacatc gacgaggccg ccgggcagaa gagaaagtac
3121 accatcaaag tcgaggtgcc caaggtggcc acccaaaccg tgggcggcgt ggagctgccc
3181 gtggccgcct ggagaagcta cctgaacatg gagctgacca tccccatctt cgccaccaac
3241 agcgactgcg agctgatcgt gaaggccatg caaggcctgc tgaaggacgg caaccccatt
3301 cctagcgcca tcgctgctaa cagcggcatt tattaacaat tgcggcaata aaaagacaga
3361 ataaaacgca cgggtgttgg gtcgtttgtt cgcggccgca ggaaccccta gtgatggagt
3421 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
3481 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
3541 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
3601 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
3661 gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc
3721 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
3781 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
3841 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
3901 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta
3961 ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat
4021 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
4081 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
4141 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
4201 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
4261 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
4321 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
4381 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
4441 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
4501 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
4561 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
4621 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
4681 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
4741 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
4801 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
4861 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
4921 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
4981 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
5041 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
5101 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
5161 cggtgagcgt ggaagccgcg gtatcattgc agcactgggg ccagatggta agccctcccg
5221 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
5281 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
5341 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
5401 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
5461 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
5521 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
5581 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
5641 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
5701 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
5761 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
5821 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
5881 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
5941 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
6001 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
6061 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
6121 gccttttgct cacatgt
//
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