pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ECPAS-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ECPAS-FLAG           7347 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7347)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..4060
                     /codon_start=1
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /product="proteasome adapter and scaffold protein 
                     ECM29isoform 3"
                     /label=ECPAS
                     /note="isoform 3 is encoded by transcript variant 3;homolog
                     of yeast Ecm29; ECM29 homolog, proteasome accessory 
                     protein; proteasome-associated protein ECM29 homolog; ecm29
                     proteasome adapter and scaffold; proteasome adapter and 
                     scaffold protein ECM29"
                     /db_xref="GI:1428713215"
                     /db_xref="CCDS:CCDS94456.1"
                     /db_xref="GeneID:23392"
                     /db_xref="HGNC:HGNC:29020"
                     /db_xref="MIM:616694"
                     /protein_id="NP_001351858.1"
                     /translation="GLGLVYELGNEQDQQELVSTLVETLMTGKRVKHEVSGETVVFQGG
                     ALGKTPDGQGLSTYKELCSLASDLSQPDLVYKFMNLANHHAMWNSRKGAAFGFNVIATR
                     AGEQLAPFLPQLVPRLYRYQFDPNLGIRQAMTSIWNALVTDKSMVDKYLKEILQDLVKN
                     LTSNMWRVRESSCLALNDLLRGRPLDDIIDKLPEIWETLFRVQDDIKESVRKAAELALK
                     TLSKVCVKMCDPAKGAAGQRTIAALLPCLLDKGMMSTVTEVRALSINTLVKISKSAGAM
                     LKPHAPKLIPALLESLSVLEPQVLNYLSLRATEQEKAAMDSARLSAAKSSPMMETINMC
                     LQYLDVSVLGELVPRLCELIRSGVGLGTKGGCASVIVSLTTQCPQDLTPYSGKLMSALL
                     SGLTDRNSVIQKSCAFAMGHLVRTSRDSSTEKLLQKLNGWYMEKEEPIYKTSCALTIHA
                     IGRYSPDVLKNHAKEVLPLAFLGMHEIADEEKSEKEECNLWTEVWQENVPGSFGGIRLY
                     LQELITITQKALQSQSWKMKAQGAIAMASIAKQTSSLVPPYLGMILTALLQGLAGRTWA
                     GKEELLKAIACVVTACSAELEKSVPNQPSTNEILQAVLKECSKENVKYKIVAISCAADI
                     LKATKEDRFQEFSNIVIPLIKKNSLESSGVRTTKNEEENEKEKELQLEYLLGAFESLGK
                     AWPRNAETQRCYRQELCKLMCERLKLSTWKVQLGVLQSMNAFFQGLMLLEEEHADPEAL
                     AEILLETCKSITYSLENKTYSSVRTEALSVIELLLKKLEESKQWECLTSECRVLLIESL
                     ATMEPDSRPELQEKAALLKKTLENLE"
     misc_feature    3279..3284
                     /label=SDS
     CDS             4067..4132
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4136..4567
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4280..4285
                     /label=SDS
     repeat_region   4610..4750
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4610..4739
                     /label=AAV2 ITR
     repeat_region   4611..4751
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4825..5280
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5562..5666
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5667..6527
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6698..7286
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggcc ttgggttggt
     1561 ttatgaacta ggcaatgaac aagatcaaca ggaattggtt tctacacttg tggaaacact
     1621 tatgactggc aaaagagtta aacatgaagt ttctggagag acagtggtat ttcaaggggg
     1681 agctcttggc aaaacaccag atggtcaggg cctttctact tacaaggaac tttgttctct
     1741 ggcaagtgat cttagccagc cagatctggt gtataaattt atgaatttag ccaaccatca
     1801 tgcaatgtgg aactctagga agggtgctgc ttttggtttt aatgtaattg ctaccagagc
     1861 tggagagcag ctggctcctt ttctgcctca gctagttcct cgactttatc gttaccagtt
     1921 tgatcccaac cttggcattc gacaggccat gacaagtatt tggaatgcgt tggtcactga
     1981 caaatccatg gtggataaat atttgaaaga aattcttcaa gatttggtta agaaccttac
     2041 aagcaatatg tggcgagttc gagaatccag ctgtttagct ttgaatgatt tattgagagg
     2101 aagaccctta gatgacatca ttgataaact tccagaaatt tgggaaacgc tttttagagt
     2161 acaagatgat atcaaggaat ctgtacgaaa agcggcagaa ctagctctga aaactctgag
     2221 caaggtctgt gtgaaaatgt gtgaccctgc caaaggagca gctggccaga gaaccatcgc
     2281 tgcccttctg ccttgccttc tggacaaagg aatgatgagc accgtgacgg aagttcgagc
     2341 cctcagcatt aacacccttg tgaagatcag caaaagtgca ggagccatgt tgaaaccgca
     2401 tgcaccaaaa ctcattccag ctctgctaga gtccttaagt gtattggagc cccaagttct
     2461 caattatttg agcctccggg cgacagagca agaaaaggct gcgatggata gtgctcggct
     2521 tagtgctgcc aaatcttctc caatgatgga aacaatcaac atgtgcctgc aataccttga
     2581 tgtgtcagtg ctgggcgagc tagttcctag gttgtgtgaa ctgatcagaa gtggtgtagg
     2641 tcttggaact aagggtggct gtgccagtgt cattgtgtca ttaactactc agtgtcctca
     2701 ggacctaaca ccttactcag gtaaacttat gagtgctttg ctgagtggcc tgacagatcg
     2761 gaacagtgtg attcagaaat cttgtgcatt tgctatgggc catttagttc ggacctcacg
     2821 ggatagcagc actgaaaaac tcctgcagaa gctcaatggg tggtatatgg agaaagaaga
     2881 acctatctac aagacctctt gtgctttgac tattcatgct attggacgat acagccctga
     2941 tgtattaaag aatcatgcaa aagaagtcct gcctctggca tttttaggca tgcatgaaat
     3001 tgctgatgag gagaaatccg aaaaagaaga atgtaattta tggaccgaag tgtggcagga
     3061 aaacgtacct ggatcctttg gtggcattcg attatacctg caggagttaa ttactattac
     3121 ccagaaggct ttgcagtctc agtcctggaa aatgaaagcc cagggtgcaa ttgccatggc
     3181 atcaattgca aaacagacta gctctctagt acctccatat ctcggaatga tactgaccgc
     3241 attgctgcaa ggcctggctg gaagaacgtg ggcaggaaag gaggagctat tgaaagccat
     3301 tgcctgtgtg gtgacagctt gcagtgcaga gctggaaaag tctgtgccca atcaacccag
     3361 cacaaatgaa attcttcaag ctgttctgaa ggaatgtagc aaagagaatg tcaaatacaa
     3421 gattgtagca atcagctgtg cagctgatat cttgaaggcc accaaagagg acagattcca
     3481 ggagttctct aacattgtca tacctctcat caagaagaac tcacttgaaa gcagtggggt
     3541 ccggacaacc aaaaatgaag aggagaatga aaaggaaaag gagctccagc tggaatatct
     3601 gctgggtgcc tttgaaagcc tgggcaaagc ctggccgcga aacgcggaga cccaacgttg
     3661 ttatcgtcag gagctgtgca aactgatgtg tgaacggcta aaactcagca cgtggaaagt
     3721 gcagctagga gtcctgcaat caatgaatgc cttttttcag gggttaatgc ttttggaaga
     3781 agaacatgcc gatcctgagg ctttggctga aattctgctt gaaacttgta aatcaatcac
     3841 atattcttta gaaaataaga cctactcatc tgtgagaaca gaagctttat ctgtgataga
     3901 attgctgctt aaaaaacttg aagaatctaa acagtgggaa tgtttgacat ctgaatgcag
     3961 agtgctccta attgagtctt tagctactat ggagccagac agcagacctg aactgcagga
     4021 gaaagcagcg ttactgaaga aaacacttga aaatctggaa tgtacagact acaaagacca
     4081 tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca agtaactcga
     4141 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4201 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4261 cgtatggctt tcattttctc ctccttgtat aaatcctggt tagttcttgc cacggcggaa
     4321 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     4381 tccgtggtgt ttatttgtga aatttgtgat gctattgctt tatttgtaac catctagctt
     4441 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     4501 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt
     4561 tttttaaaga tctggagacc ggtgtggcac gtgcggaccg agcggccgca ggaaccccta
     4621 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     4681 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     4741 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     4801 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4861 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg
     4921 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4981 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5041 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5101 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta
     5161 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa
     5221 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     5281 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     5341 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     5401 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     5461 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     5521 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5581 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5641 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5701 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5761 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5821 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5881 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5941 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6001 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6061 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6121 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6181 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6241 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6301 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6361 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6421 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6481 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6541 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6601 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6661 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6721 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6781 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6841 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6901 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6961 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7021 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7081 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7141 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7201 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7261 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7321 ccttttgctg gccttttgct cacatgt
//