| 质粒名称: | pGL3-G/S/A-Nicking/EpegRNA-PGK-puroR-mRFP1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002190 |
| 产品用途: | hCtRNA simultaneously drives the expression of NickingRNA and EpegRNA targeting three different genes: GATA2, SETBP1, ASXL1, harboring dual selection markers of puroR and RFP. |
| 存储实验室: | Zhuobin Liang Lab 梁卓斌课题组 |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3 |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
hCtRNA
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | GATA2-EpegRNA-nicking-sgRNA, SETBP1-EpegRNA-nicking-sgRNA, ASXL1-EpegRNA-nicking-sgRNA |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
TRU-PE: A Universal, Trackable Prime Editor Toolkit for Robust Single-and Multi-Locus Genome Engineering
Zhichao Qiu, Keke Sun, Qingwei Zeng, Ziwei Luo, Xinran Liu, Yaping Li, Xiang Lei, Ruilin Zhao, Zhen Zhang, Damia Romero-Moya, Yuan Yang, Xuan Wang, Ehsan Hashemi, Joe Z. Zhang, Xiaolong Wang, Alessandra Giorgetti, Zhuobin Liang
bioRxiv 2026.02.20.706938
|
pGL3-G/S/A-Nicking/EpegRNA-PGK-puroR-mRFP1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pGL3-G/S/A-Nicking/EpegRNA-PGK-puroR-mRFP1载体质粒基本信息
pGL3-G/S/A-Nicking/EpegRNA-PGK-puroR-mRFP1质粒图谱载体图谱和pGL3-G/S/A-Nicking/EpegRNA-PGK-puroR-mRFP1载体序列质粒序列多克隆位点信息
pGL3-G/S/A-Nicking/EpegRNA-PGK-puroR-mRFP1质粒载体简介
pGL3-G/S/A-Nicking/EpegRNA-PGK-puroR-mRFP1质粒序列载体序列
LOCUS pGL3-G/S/A-Nicking/EpegRNA-PGK-puroR-mRFP1 6622 bp DNA circular SYN 07-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6622)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6622
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 1..20
/label=OL2714
primer_bind 4..23
/label=OL1915
primer_bind 4..21
/label=OL1400
primer_bind 12..36
/label=OL2631
primer_bind complement(12..36)
/label=OL2637
primer_bind 18..40
/label=OL2707
primer_bind 21..55
/label=OL2640
primer_bind 37..61
/label=OL1451
misc_feature 41..115
/label=hCtRNA
primer_bind complement(98..144)
/label=OL2595
primer_bind complement(98..117)
/label=OL2608
primer_bind complement(98..116)
/label=OL2596
primer_bind complement(98..115)
/label=OL2600
primer_bind complement(98..115)
/label=OL2602
primer_bind complement(98..115)
/label=OL2606
misc_feature 116..135
/label=GATA2 nickingRNA
primer_bind complement(124..135)
/label=OL2632
primer_bind 135..171
/label=OL2603
primer_bind complement(136..215)
/label=OL476
misc_RNA 136..211
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 136..207
/label=OL2116
primer_bind 136..171
/label=OL2597
primer_bind 136..171
/label=OL2601
primer_bind 136..171
/label=OL2607
primer_bind 136..171
/label=OL2609
primer_bind complement(140..211)
/label=OL2117
primer_bind 140..177
/label=OL1453
primer_bind complement(183..202)
/label=OL1927
primer_bind complement(188..207)
/label=OL1940
primer_bind complement(194..212)
/label=OL2619
primer_bind complement(194..211)
/label=OL2615
primer_bind complement(194..211)
/label=OL2611
primer_bind complement(194..211)
/label=OL769
primer_bind 208..286
/label=OL477
misc_feature 212..286
/label=hCtRNA
primer_bind 212..232
/label=OL1451
primer_bind complement(269..298)
/label=OL2596
primer_bind complement(269..287)
/label=OL2595
primer_bind complement(269..287)
/label=OL2608
primer_bind complement(269..286)
/label=OL2600
primer_bind complement(269..286)
/label=OL2602
primer_bind complement(269..286)
/label=OL2606
misc_feature 287..306
/label=GATA2 spacer
primer_bind 295..342
/label=OL2597
misc_RNA 307..382
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 307..378
/label=OL2116
primer_bind 307..342
/label=OL2601
primer_bind 307..342
/label=OL2603
primer_bind 307..342
/label=OL2607
primer_bind 307..342
/label=OL2609
primer_bind complement(311..382)
/label=OL2117
primer_bind 311..348
/label=OL1453
primer_bind complement(354..373)
/label=OL1927
primer_bind complement(359..378)
/label=OL1940
primer_bind complement(365..406)
/label=OL2611
primer_bind complement(365..382)
/label=OL2615
primer_bind complement(365..382)
/label=OL2619
primer_bind complement(365..382)
/label=OL769
misc_feature 383..394
/label=RTT
primer_bind complement(388..445)
/label=OL2632
misc_feature 395..410
/label=PBS
primer_bind 403..449
/label=OL2613
primer_bind complement(407..453)
/label=OL2614
misc_feature 411..418
/label=Linker
primer_bind complement(419..464)
/label=OL2621
primer_bind 419..460
/label=OL2620
misc_feature 419..455
/label=motif
primer_bind complement(419..447)
/label=OL2634
primer_bind complement(419..447)
/label=OL2635
primer_bind complement(419..446)
/label=OL2618
primer_bind 419..442
/label=OL2617
primer_bind 424..481
/label=OL2633
primer_bind 427..478
/label=OL2636
primer_bind 443..482
/label=OL2616
primer_bind 449..482
/label=OL2612
primer_bind 479..495
/label=OL2707
primer_bind 492..516
/label=OL1451
misc_feature 496..570
/label=hCtRNA
primer_bind complement(553..586)
/label=OL2600
primer_bind complement(553..570)
/label=OL2595
primer_bind complement(553..570)
/label=OL2596
primer_bind complement(553..570)
/label=OL2602
primer_bind complement(553..570)
/label=OL2606
primer_bind complement(553..570)
/label=OL2608
misc_feature 571..590
/label=SETBP1 nickingRNA
primer_bind 574..626
/label=OL2601
primer_bind 588..626
/label=OL2607
primer_bind 590..626
/label=OL2609
primer_bind complement(591..670)
/label=OL476
misc_RNA 591..666
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 591..662
/label=OL2116
primer_bind 591..626
/label=OL2597
primer_bind 591..626
/label=OL2603
primer_bind complement(595..666)
/label=OL2117
primer_bind 595..632
/label=OL1453
primer_bind complement(638..657)
/label=OL1927
primer_bind complement(643..662)
/label=OL1940
primer_bind complement(649..667)
/label=OL2619
primer_bind complement(649..666)
/label=OL2615
primer_bind complement(649..666)
/label=OL2611
primer_bind complement(649..666)
/label=OL769
primer_bind 663..741
/label=OL477
misc_feature 667..741
/label=hCtRNA
primer_bind 667..687
/label=OL1451
primer_bind complement(724..770)
/label=OL2595
primer_bind complement(724..755)
/label=OL2602
primer_bind complement(724..741)
/label=OL2596
primer_bind complement(724..741)
/label=OL2600
primer_bind complement(724..741)
/label=OL2606
primer_bind complement(724..741)
/label=OL2608
misc_feature 742..761
/label=SETBP1 spacer
primer_bind 752..797
/label=OL2603
misc_RNA 762..837
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 762..833
/label=OL2116
primer_bind 762..797
/label=OL2597
primer_bind 762..797
/label=OL2601
primer_bind 762..797
/label=OL2607
primer_bind 762..797
/label=OL2609
primer_bind complement(766..837)
/label=OL2117
primer_bind 766..803
/label=OL1453
primer_bind complement(809..828)
/label=OL1927
primer_bind complement(814..833)
/label=OL1940
primer_bind complement(820..861)
/label=OL2615
primer_bind complement(820..837)
/label=OL2619
primer_bind complement(820..837)
/label=OL2611
primer_bind complement(820..837)
/label=OL769
misc_feature 838..848
/label=RTT
primer_bind complement(843..898)
/label=OL2634
misc_feature 849..861
/label=PBS
primer_bind 856..893
/label=OL2617
primer_bind complement(860..897)
/label=OL2618
misc_feature 862..869
/label=Linker
primer_bind complement(870..915)
/label=OL2621
primer_bind 870..911
/label=OL2620
misc_feature 870..906
/label=motif
primer_bind complement(870..904)
/label=OL2614
primer_bind 870..900
/label=OL2613
primer_bind complement(870..898)
/label=OL2635
primer_bind complement(870..896)
/label=OL2632
primer_bind 875..932
/label=OL2633
primer_bind 878..929
/label=OL2636
primer_bind 894..933
/label=OL2616
primer_bind 900..933
/label=OL2612
primer_bind 930..946
/label=OL2707
primer_bind 943..967
/label=OL1451
misc_feature 947..1021
/label=hCtRNA
misc_feature 947..949
/label=5' leader
primer_bind complement(1004..1038)
/label=OL2606
primer_bind complement(1004..1021)
/label=OL2595
primer_bind complement(1004..1021)
/label=OL2596
primer_bind complement(1004..1021)
/label=OL2600
primer_bind complement(1004..1021)
/label=OL2602
primer_bind complement(1004..1021)
/label=OL2608
misc_feature 1022..1041
/label=ASXL1 nickingRNA
primer_bind 1032..1077
/label=OL2607
primer_bind 1039..1077
/label=OL2601
primer_bind 1041..1077
/label=OL2609
primer_bind complement(1042..1121)
/label=OL476
misc_RNA 1042..1117
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 1042..1113
/label=OL2116
primer_bind 1042..1077
/label=OL2597
primer_bind 1042..1077
/label=OL2603
primer_bind complement(1046..1117)
/label=OL2117
primer_bind 1046..1083
/label=OL1453
primer_bind complement(1089..1108)
/label=OL1927
primer_bind complement(1094..1113)
/label=OL1940
primer_bind complement(1100..1118)
/label=OL2619
primer_bind complement(1100..1117)
/label=OL2615
primer_bind complement(1100..1117)
/label=OL2611
primer_bind complement(1100..1117)
/label=OL769
primer_bind 1114..1192
/label=OL477
misc_feature 1118..1192
/label=hCtRNA
primer_bind 1118..1138
/label=OL1451
primer_bind complement(1175..1208)
/label=OL2608
primer_bind complement(1175..1194)
/label=OL2595
primer_bind complement(1175..1193)
/label=OL2596
primer_bind complement(1175..1192)
/label=OL2600
primer_bind complement(1175..1192)
/label=OL2602
primer_bind complement(1175..1192)
/label=OL2606
misc_feature 1193..1212
/label=ASXL1 spacer
primer_bind 1203..1248
/label=OL2609
primer_bind 1212..1248
/label=OL2601
primer_bind 1212..1248
/label=OL2607
misc_RNA 1213..1288
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 1213..1284
/label=OL2116
primer_bind 1213..1248
/label=OL2597
primer_bind 1213..1248
/label=OL2603
primer_bind complement(1217..1288)
/label=OL2117
primer_bind 1217..1254
/label=OL1453
primer_bind complement(1260..1279)
/label=OL1927
primer_bind complement(1265..1284)
/label=OL1940
primer_bind complement(1271..1312)
/label=OL2619
primer_bind complement(1271..1288)
/label=OL2615
primer_bind complement(1271..1288)
/label=OL2611
primer_bind complement(1271..1288)
/label=OL769
misc_feature 1289..1302
/label=RTT
primer_bind complement(1294..1347)
/label=OL2635
misc_feature 1303..1310
/label=PBS
primer_bind 1308..1360
/label=OL2620
misc_feature 1311..1318
/label=Linker
primer_bind complement(1312..1364)
/label=OL2621
misc_feature 1319..1355
/label=motif
primer_bind complement(1319..1353)
/label=OL2614
primer_bind 1319..1349
/label=OL2613
primer_bind complement(1319..1347)
/label=OL2634
primer_bind complement(1319..1346)
/label=OL2618
primer_bind complement(1319..1345)
/label=OL2632
primer_bind 1319..1342
/label=OL2617
primer_bind 1324..1378
/label=OL2633
primer_bind 1327..1385
/label=OL2636
primer_bind complement(1368..1387)
/label=OL1916
primer_bind complement(1382..1398)
/label=OL1168
misc_feature 1399..1516
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
primer_bind complement(1453..1472)
/label=OL1936
primer_bind complement(1459..1483)
/label=OL1571
primer_bind complement(1535..1554)
/label=OL1088
promoter 1565..2075
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind complement(1659..1678)
/label=OL1068
primer_bind 1807..1829
/label=OL1709
primer_bind 1849..1865
/label=OL446
primer_bind 1994..2012
/label=hPGK-F
/note="Human PGK promoter, forward primer"
primer_bind complement(2077..2096)
/label=OL2256
primer_bind complement(2078..2099)
/label=OL1702
CDS 2097..2693
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(2097..2116)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 2100..2119
/label=OL2264
primer_bind 2593..2613
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind 2593..2612
/label=OL2294
primer_bind 2593..2611
/label=OL2339
primer_bind 2664..2718
/label=OL901
primer_bind complement(2670..2693)
/label=OL306
primer_bind complement(2679..2701)
/label=OL384
primer_bind 2686..2717
/label=OL379
CDS 2694..2750
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
primer_bind 2694..2750
/label=OL866
primer_bind complement(2694..2750)
/label=OL867
primer_bind 2694..2720
/label=OL2251
primer_bind 2731..2774
/label=OL902
primer_bind complement(2731..2758)
/label=OL966
RBS 2733..2740
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 2751..3428
/codon_start=1
/product="monomeric derivative of DsRed (Campbell et al.,
2002)"
/label=mRFP1
/translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
TGA"
primer_bind 2751..2769
/label=OL2300
primer_bind 2982..3001
/label=OL1123
primer_bind complement(2991..3017)
/label=OL1728
primer_bind complement(3023..3049)
/label=OL2455
primer_bind complement(3406..3453)
/label=OL903
primer_bind complement(3406..3428)
/label=OL2353
primer_bind complement(3407..3428)
/label=OL2708
primer_bind 3427..3445
/label=OL965
primer_bind 3429..3451
/label=OL305
polyA_signal 3575..3696
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind 3575..3611
/label=OL1698
primer_bind complement(3586..3605)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 3640..3659
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(3680..3712)
/label=OL2209
primer_bind 3697..3730
/label=OL1692
primer_bind complement(3710..3736)
/label=OL1941
primer_bind complement(3885..3908)
/label=OL1043
primer_bind 3886..3908
/label=OL1044
primer_bind complement(3944..3961)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4115..4703)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4195..4214)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind complement(4297..4316)
/label=OL1041
primer_bind complement(4430..4449)
/label=OL631
primer_bind 4486..4505
/label=OL798
primer_bind complement(4727..4766)
/label=OL660
primer_bind 4747..4766
/label=OL659
CDS complement(4874..5734)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5331..5350
/label=OL1922
primer_bind 5497..5516
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
primer_bind complement(5497..5516)
/label=OL1923
primer_bind 5650..5669
/label=OL1033
primer_bind complement(5650..5669)
/label=OL1034
promoter complement(5735..5839)
/gene="bla"
/label=AmpR promoter
rep_origin 5866..6321
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5891..5900)
/label=OL305
primer_bind complement(5953..5972)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6163..6184
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 6374..6393
/label=OL1469
primer_bind 6383..6399
/label=OL1109
polyA_signal 6452..6500
/note="synthetic polyadenylation signal"
misc_feature 6514..6605
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 6514..6530
/label=OL1167
primer_bind 6560..6579
/label=OL1087
primer_bind 6584..6598
/label=OL1935
primer_bind join(6614..6622,1..17)
/label=OL1700
ORIGIN
1 agtgaacgga tctcgacggt atcgatcacg agactagcct agagggggta tagctcagtg
61 gtagagcatt tgactgcaga tcaagaggtc cccggttcaa atccgggtgc cccctacatc
121 ttccggttct gagtcgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
181 tcaacttgaa aaagtggcac cgagtcggtg cagagggggt atagctcagt ggtagagcat
241 ttgactgcag atcaagaggt ccccggttca aatccgggtg ccccctagaa ggaagggatc
301 cagactgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
361 aaaagtggca ccgagtcggt gctcttccgg ttctgagtct ggatcccttc aacaaacgcg
421 cggttctatc tagttacgcg ttaaaccaac tagaattttt ttctcgacct cgagacaaga
481 tcacgagact agcctagagg gggtatagct cagtggtaga gcatttgact gcagatcaag
541 aggtccccgg ttcaaatccg ggtgccccct gagacgatcc ccagcaacag gttttagagc
601 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
661 cggtgcagag ggggtatagc tcagtggtag agcatttgac tgcagatcaa gaggtccccg
721 gttcaaatcc gggtgccccc ttgtcccaat gccgctgtcg cgttttagag ctagaaatag
781 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcgat
841 ccccagcaac agcggcattg gttaaatatc gcggttctat ctagttacgc gttaaaccaa
901 ctagaatttt tttctcgacc tcgagacaag atcacgagac tagcctagag ggggtatagc
961 tcagtggtag agcatttgac tgcagatcaa gaggtccccg gttcaaatcc gggtgccccc
1021 tccccccccc tccgatggca ggttttagag ctagaaatag caagttaaaa taaggctagt
1081 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcaga gggggtatag ctcagtggta
1141 gagcatttga ctgcagatca agaggtcccc ggttcaaatc cgggtgcccc ctactgccat
1201 cggagggggg gggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
1261 acttgaaaaa gtggcaccga gtcggtgcac ccgggccacc ccccccctcc actttctccg
1321 cggttctatc tagttacgcg ttaaaccaac tagaattttt ttctcgacct cgagacaaat
1381 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
1441 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
1501 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggccgcggct
1561 cgagggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
1621 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggactcgc acattcttca
1681 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
1741 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
1801 aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg
1861 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga
1921 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt
1981 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct
2041 ccctcgttga ccgaatcacc gacctctctc cccaggggga tccaccggag cttaccatga
2101 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
2161 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
2221 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
2281 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
2341 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
2401 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
2461 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
2521 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaaa
2581 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
2641 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccgctacta
2701 acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct atggcgagta
2761 gcgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg
2821 gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg
2881 ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc
2941 agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga
3001 aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg
3061 ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac
3121 tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg
3181 aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc
3241 gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta
3301 aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc
3361 acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg
3421 gtgcttaacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
3481 catcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttctag
3541 agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa
3601 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
3661 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
3721 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
3781 tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca acccagtcag
3841 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
3901 catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc
3961 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
4021 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
4081 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
4141 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
4201 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
4261 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
4321 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
4381 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
4441 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
4501 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
4561 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
4621 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
4681 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
4741 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
4801 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
4861 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
4921 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
4981 taccatctgg ccccagtgct gcaatgatac cgcgggaccc acgctcaccg gctccagatt
5041 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
5101 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
5161 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
5221 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
5281 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
5341 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
5401 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
5461 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
5521 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
5581 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
5641 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
5701 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
5761 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
5821 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg
5881 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
5941 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
6001 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
6061 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
6121 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
6181 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
6241 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
6301 aaatattaac gcttacaatt tgccattcgc cattcaggct gcgcaactgt tgggaagggc
6361 gatcggtgcg ggcctcttcg ctattacgcc agcccaagct accatgataa gtaagtaata
6421 ttaaggtacg ggaggtactt ggagcggccg caataaaata tctttatttt cattacatct
6481 gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc aaaacaaaac
6541 gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg ccagaacatt
6601 tctctatcga taggtaccga tt
//
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