| 质粒名称: | pADH137 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 90986 |
| 存储实验室: | Aaron Hernday |
|
载体骨架基本信息 |
|
| 载体名称: | pUC19 |
| 载体出品公司: | Clontech |
| 空载体大小: | 2242bp |
| 完整质粒大小: | 9184bp |
| 载体类型: | CRISPR |
| 筛选标记: | Nourseothricin (NAT) |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称1: | C. albicans LEU2 1 of 2 |
| 插入大小: | 750bp |
| 插入基因名称2: | pENO1 |
| 插入大小: | 783bp |
| 插入基因名称3: | Cas9 |
| 插入大小: | 4113bp |
| 插入基因名称4: | NAT 1 of 2 |
| 插入大小:: | 842 bp |
|
文献引用方法 |
|
| 材料和方法部分: | pADH137 was a gift from Aaron Hernday (Addgene plasmid # 90986 ; http://n2t.net/addgene:90986 ; RRID:Addgene_90986) |
| 参考文献部分: | An Efficient, Rapid, and Recyclable System for CRISPR-Mediated Genome Editing in Candida albicans. Nguyen N, Quail MMF, Hernday AD. mSphere. 2017 Apr 26;2(2):e00149-17. doi: 10.1128/mSphereDirect.00149-17. eCollection 2017 Mar-Apr. 10.1128/mSphereDirect.00149-17 PubMed 28497115 |
买家导航
pADH137载体质粒基本信息
pADH137质粒图谱载体图谱和pADH137载体序列质粒序列多克隆位点信息
pADH137质粒载体简介
pADH137质粒序列载体序列
LOCUS pADH137 9185 bp DNA circular SYN 14-APR-2025
DEFINITION NAT-marked CAS9 expression construct; part 1 of 2 of C.alb LEUpOUT
CRISPR system. Use with pADH118-series gRNA expression constructs..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9185)
AUTHORS Nguyen N, Quail MMF, Hernday AD
TITLE An Efficient, Rapid, and Recyclable System for CRISPR-Mediated
Genome Editing in Candida albicans.
JOURNAL mSphere. 2017 Apr 26;2(2):e00149-17. doi:
10.1128/mSphereDirect.00149-17. eCollection 2017 Mar-Apr.
PUBMED 28497115
REFERENCE 2 (bases 1 to 9185)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1128/mSphereDirect.00149-17"; journalName: "mSphere"; date:
"2017-04-26- 26"; volume: "2"; issue: "2"; pages: "e00149-17"
COMMENT Sequence Label: pADH137
FEATURES Location/Qualifiers
source 1..9185
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 138..156
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
CDS 1724..5827
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 1724..5827
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 5840..5860
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5864..5884
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5888..5908
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5909..5974
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 6039..6286
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
primer_bind complement(6055..6073)
/label=CYC1
/note="CYC1 transcription termination signal, reverse
primer"
promoter 6326..6669
/label=TEF promoter
/note="Ashbya gossypii TEF promoter"
promoter complement(join(7177..7183,7184..7201,7202..7207))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7222..7243
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7360..7377)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7531..8119)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7611..7630)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(8290..9081,9082..9150))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8913..8932
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(join(9151..9185,1..70))
/gene="bla"
/label=AmpR promoter
ORIGIN
1 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
61 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
121 tgacattaac ctataaaaat aggcgtatca cgaggccctt tgacggcacg gccacgcgtt
181 taaaccgcca tgtctgttaa aaccaaaacc attactgtct tgcctggtga ccatgtcggt
241 accgaaattg tcaatgaagc aatcaaagtc ttgaaagcaa ttgaagctgc cactccctac
301 caaaaaatcc aatttgattt caaacaccac ttgattggtg gtgctgccat tgatgccact
361 ggcgtcccgc ttccagatga tgctcttgaa agtgctaaat catccgatgc agttttgttg
421 ggtgctgttg gtggacccaa atggggtact ggcactgttc gtcctgaaca aggattattg
481 aaaatccgta aagaattgaa cctttatgcc aacattagac catgtaattt tgccagtgac
541 tcgttattag aattgtctcc attaaaagct gaagtggtca aaggaaccaa tttaattatt
601 gttcgtgaat tagttggtgg tatatatttt ggtgaacgtc aagaacaaga agaaagtgaa
661 gacaaaaaaa cagcttggga tacagaaaag tatactgttg acgaagttac tcgtatcact
721 cgtatggcag catttatggc tttgcagcac aatccaccat tgccaatatg gtctttggac
781 aaagctaatg ttttggcatc gtcgagattg tggagaaaga ccgttgataa agtgatttct
841 gaagaatttc cagctttgtc tgtgcagcat caattgattg attcggctgc aatgattttg
901 atccaaaacc caaccaaatt gaacggtatc atcattacaa cagctaacaa ttacacaaaa
961 aaaattctct tttcgatgta gcaactatct tcaactaaaa cattttctcc ttcggcccat
1021 gattgtcctc cgggtcgaca gcaagccgtt acaattgaga tggaaagcga cctaccttca
1081 ctcgataagg tgcttaattg tacttcatat aaatctggcc cggatctaaa caaatgagtt
1141 ccattaagcc gtgggttctc aattagggtt tttgtttttg atttagaaaa aagagatcaa
1201 gatttgttta caggtgatgc ctttttttag aacttatgcg ttgcaaaagt tgactaacga
1261 tttctataag gtgatccaca ctaattatac aaacgtacaa acagacatac ttttcctgcg
1321 ttcacctgat gttggccaga tttctctctt cattgcatag aacataacca cactagggca
1381 acagaaaaaa aaaaaaaagt gcatcgggaa gttgtgttcc attcattata tgtctactac
1441 tgcatatgag tagcccaccc accaccacca tagtaagttt ttgtgtatgc gcgccgtcag
1501 gttatttcat ttctgaattt ttcaaccacc ttactccctt tattgttgat tgacaatttt
1561 gctcacagta agatctttta gactccaatt aatataaaat aagtctgatt ttccaattcc
1621 tgttttttct ttttttttct gtttctattt ctttcctttt ctcccttttt tttaattctt
1681 cattcaatca tcaattgata attcaggaat attacaaccc gggatggata aaaagtatag
1741 tattggttta gatattggta ctaactctgt gggttgggca gttatcactg acgaatataa
1801 agttccatca aagaaattta aggtgttagg taacactgac agacactcaa taaaaaagaa
1861 tcttatcggt gctcttttgt tcgactccgg tgaaactgcc gaggctacac gtttaaaaag
1921 aacagcaaga agaagatata cccgtagaaa aaatagaata tgttatttac aagaaatctt
1981 ttctaatgaa atggctaaag ttgatgattc ctttttccat agattggaag agtcattttt
2041 ggttgaagaa gacaaaaagc atgagagaca tccaatcttt gggaatatag ttgatgaagt
2101 ggcttaccat gaaaaatatc ctaccattta tcatttaaga aagaaattgg tagattcaac
2161 tgataaagct gaccttagat taatctattt agcacttgcc catatgatta aatttagagg
2221 tcattttttg attgaaggtg atttgaaccc agataattct gacgtggata aattatttat
2281 tcaattagtc caaacctaca accaattatt tgaggaaaat ccaattaatg ctagtggtgt
2341 cgatgccaaa gctatattat cagccagatt atcaaaatct agacgtttgg aaaatttgat
2401 tgcccaattg ccaggagaaa aaaagaatgg attatttgga aacttgatcg cattatcatt
2461 gggtttgaca ccaaatttta aatctaattt tgatttagct gaagatgcta aattacaatt
2521 atcaaaagac acctatgacg acgatttgga caatttactt gctcaaattg gtgatcaata
2581 tgcagatttg ttcttagctg ctaaaaactt atctgatgct attttgttgt ctgatatttt
2641 gagagtgaac acagaaataa ccaaagctcc attatcagca tctatgatca aacgttatga
2701 tgaacaccat caggatttga ctttattgaa agctttggtg agacaacaat tgccagagaa
2761 gtataaagaa atctttttcg atcaatctaa aaacgggtat gcaggttata ttgatggggg
2821 tgcctcccaa gaggaatttt acaaatttat aaaacctatt ttagaaaaga tggatgggac
2881 tgaggaactt ttggtcaaat tgaacagaga agatttgtta cgtaaacaga gaacttttga
2941 taatggtagt atacctcacc aaattcattt gggtgagttg catgcaattt taagaagaca
3001 agaagatttt tatccatttt taaaagataa tagagaaaaa atcgagaaaa ttttaacctt
3061 tagaattcca tactatgttg ggcctttggc tagaggtaat tcaagatttg cctggatgac
3121 acgtaaatca gaagaaacta ttaccccttg gaattttgaa gaggttgttg ataaaggagc
3181 atcagcacag agttttattg aaagaatgac caatttcgat aaaaacttac caaatgaaaa
3241 agttttacca aaacattcct tgttatacga atattttact gtttacaatg aacttacaaa
3301 ggttaaatat gttactgaag gtatgcgtaa gccagccttt ttatctggag aacagaaaaa
3361 ggcaatagtt gatttattgt ttaaaacaaa tagaaaagtt actgttaaac aattaaaaga
3421 agattacttt aagaaaattg aatgttttga ttcagttgaa atcagtggtg ttgaagacag
3481 atttaatgct agtttaggaa cttaccatga tttacttaaa attatcaaag ataaagattt
3541 cttggataac gaagaaaatg aagacatttt agaagacatt gttttaacct taactttatt
3601 cgaagataga gagatgattg aagaacgttt gaagacttat gcacatttgt ttgacgataa
3661 agtgatgaaa cagttgaaaa gaagacgtta tactggatgg ggtagattgt ctcgtaaatt
3721 gatcaatgga attagagata aacaaagtgg taaaactatc ttggactttt tgaaatctga
3781 cggatttgct aatagaaatt tcatgcaatt gatccacgac gatagtttga catttaaaga
3841 agacatccaa aaggcccaag tgagtgggca aggtgattca ttacatgaac atattgcaaa
3901 tttagccgga tctcctgcta ttaagaaagg gatattacaa actgttaaag ttgtggatga
3961 attagtgaaa gtaatgggaa gacataaacc tgaaaacatt gtcattgaga tggcaagaga
4021 aaatcaaact acacaaaaag gacagaaaaa tagtagagaa cgtatgaaaa gaatagaaga
4081 gggtattaaa gaattgggta gtcaaatatt gaaagaacac ccagtggaaa atacccagtt
4141 gcaaaatgaa aaattatatc tttactacct tcaaaatgga cgtgatatgt atgttgatca
4201 ggaattagat ataaatagac tttcagatta tgatgtagat catatagttc cacaatcttt
4261 cttgaaagat gattccatag acaataaagt attaactaga agtgataaaa atagaggtaa
4321 aagtgataat gtcccaagtg aggaagtcgt caaaaagatg aaaaattact ggcgtcaact
4381 tttgaatgct aaattaatta ctcaaagaaa atttgataat ttgactaaag cagaaagagg
4441 tgggctttct gaattagata aagccgggtt cattaaaaga caattggtcg aaactagaca
4501 aattactaaa catgttgccc aaattttaga ttcccgtatg aacactaagt atgacgaaaa
4561 tgataagtta atacgtgagg ttaaagtcat tactttaaaa tcaaaacttg tctctgattt
4621 cagaaaggat ttccaattct ataaagttag agaaattaat aattatcatc atgctcatga
4681 tgcatatttg aatgctgtag ttggaactgc tttaatcaag aaatacccta aattagaatc
4741 tgaatttgta tatggtgatt acaaagtcta tgatgttaga aagatgattg ctaaatcaga
4801 acaagaaatt ggtaaagcta cagctaaata cttcttttac tctaacatta tgaatttctt
4861 taaaacagaa attactttgg caaacggtga aattagaaaa agacctctta ttgaaacaaa
4921 tggtgagact ggagagatag tttgggacaa agggcgtgat ttcgctactg ttagaaaagt
4981 tttatcaatg ccacaagtta acattgtaaa gaaaacagag gttcaaactg gtggtttctc
5041 aaaagaaagt attttgccta aaagaaatag tgataaattg attgccagaa aaaaggattg
5101 ggatccaaag aaatatggtg gtttcgactc accaaccgta gcctattctg ttttggttgt
5161 ggcaaaggtt gaaaagggta aaagtaaaaa gcttaaatca gtaaaagaac ttttgggtat
5221 tacaataatg gaaagaagtt cctttgaaaa gaaccctatt gattttttgg aagctaaagg
5281 ttataaggaa gtaaagaagg acttaataat caaattgcct aaatattctt tatttgaatt
5341 agaaaatggg agaaaaagaa tgttggcttc tgctggagaa ttgcaaaagg gtaatgaatt
5401 agcattgcct tccaaatatg ttaacttctt gtatttagct tcacactatg aaaagttgaa
5461 agggtcacca gaagataacg agcaaaaaca attatttgtt gaacaacaca aacactactt
5521 agatgagatt atagaacaaa ttagtgaatt cagtaaaaga gtgatattag ctgatgcaaa
5581 tttagataaa gttttgtcag cctataacaa acatagagat aagccaatta gagaacaagc
5641 agaaaacatt attcacttat ttacccttac caatttagga gcacctgctg ctttcaagta
5701 ttttgataca acaattgatc gtaaaagata tacctcaaca aaagaagtct tagacgccac
5761 cttaattcat caatcaatca ctggattgta tgagacaaga attgatttgt ctcaattggg
5821 tggtgatgaa ggggctgatc ctaagaagaa aagaaaagtt gatccaaaga aaaagcgtaa
5881 ggtggatcct aagaaaaaga gaaaggttga ctacaaagac catgacggtg attataaaga
5941 tcatgacatc gactacaagg atgacgatga caagtgataa tgactgcaga gatccatcga
6001 cctgccgcca agctaattcc gggcgaattt ctgtcgagtc atgtaattag ttatgtcacg
6061 cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc
6121 tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat
6181 atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa
6241 aaccttgctt tagaaggttt tgggacgctc gaaggcttta atttgcggcc agcttgcctc
6301 gtccccgcct ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct
6361 tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc
6421 ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta
6481 cggctcctcg ctgcagacct gcgagcaggg aaacgctccc ctcacagacg cgttgaattg
6541 tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac tgaggttctt
6601 ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca catccgaaca
6661 taaacaaccg gatcctacaa ttatgtctac tactttggat gatactgctt atagatacag
6721 aacttctgtt ccaggtgatg ctgaagctat tgaagctttg gatggttctt tcactaccga
6781 tactgttttt agagttactg ctactggtga tggtttcact ttgagagaag ttccagttga
6841 tccaccattg actaaggttt tcccagatga tgaatccgat gatgaatccg atgctggtga
6901 agatggtgat ccagattcta gaactttcgt tgcttatggt gatgatggtg atttggctgg
6961 tttcgttgtt gtttcttatt ctggttggaa cagaagattg actgttgaag atattgaagt
7021 tgctccagaa catagaggtc atggtgttgg tagagctttg atgggtttgg ctactgaatt
7081 cgccagagaa agaggtgctg gtcatttgtg gttggaagtt accaatgtta atcggtgttt
7141 aaaccccagc gcctggcggg cgctcacaat tccacacaac atacgagccg gaagcataaa
7201 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
7261 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
7321 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
7381 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
7441 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
7501 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
7561 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
7621 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
7681 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
7741 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
7801 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
7861 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
7921 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
7981 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
8041 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
8101 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
8161 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
8221 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
8281 gtctgacagt taccaatgct tgatcagtga ggcacctatc tcagcgatct gtctatttcg
8341 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
8401 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
8461 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
8521 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
8581 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
8641 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
8701 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
8761 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
8821 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
8881 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
8941 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
9001 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
9061 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
9121 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
9181 ttatc
//
