pCMV-nCas9-G7

LOCUS       pCMV-nCas9-G7      8160 bp DNA     circular SYN 07-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(38..76)
                     /label=OL4014
     enhancer        87..466
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     87..105
                     /label=OL4271
     primer_bind     101..128
                     /label=OL1151
     primer_bind     complement(106..130)
                     /label=OL1047
     primer_bind     complement(168..195)
                     /label=OL1815
     primer_bind     318..355
                     /label=OL1892
     primer_bind     331..353
                     /label=OL4042
     primer_bind     complement(350..370)
                     /label=OL 5075
     primer_bind     388..407
                     /label=OL064
     primer_bind     437..466
                     /label=OL4541
     promoter        467..670
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     569..588
                     /label=OL790
     primer_bind     615..636
                     /label=OL096
     primer_bind     621..641
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     621..640
                     /label=OL1335
     primer_bind     complement(622..639)
                     /label=OL400
     primer_bind     622..639
                     /label=OL443
     primer_bind     632..647
                     /label=OL4359
     primer_bind     640..663
                     /label=OL152
     primer_bind     640..663
                     /label=OL159
     primer_bind     648..669
                     /label=OL 4755
     primer_bind     649..671
                     /label=OL084
     primer_bind     675..716
                     /label=OL4013
     primer_bind     693..725
                     /label=OL2387
     primer_bind     709..729
                     /label=OL3918
     primer_bind     712..731
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        712..730
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     712..730
                     /label=OL1124
     primer_bind     complement(727..746)
                     /label=OL1206
     primer_bind     complement(730..743)
                     /label=OL4272
     primer_bind     complement(731..820)
                     /label=OL1393
     primer_bind     complement(731..743)
                     /label=OL3698
     primer_bind     760..774
                     /label=OL2802
     primer_bind     complement(765..779)
                     /label=OL2538
     primer_bind     complement(774..800)
                     /label=OL1013
     primer_bind     complement(778..800)
                     /label=OL1036
     CDS             780..800
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     781..822
                     /label=OL1039
     primer_bind     781..820
                     /label=OL1296
     primer_bind     complement(781..800)
                     /label=OL1298
     CDS             801..4901
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=nCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(805..824)
                     /label=OL1051
     primer_bind     complement(827..843)
                     /label=OL 5142
     primer_bind     1029..1053
                     /label=OL2715
     primer_bind     complement(1029..1053)
                     /label=OL2716
     primer_bind     complement(1249..1268)
                     /label=OL1336
     primer_bind     1269..1283
                     /label=OL1386
     primer_bind     complement(1273..1291)
                     /label=OL3674
     primer_bind     complement(1337..1356)
                     /label=OL989
     primer_bind     complement(1409..1419)
                     /label=OL900
     primer_bind     complement(1442..1471)
                     /label=OL1321
     primer_bind     1447..1476
                     /label=OL1322
     primer_bind     1493..1512
                     /label=OL 4742
     primer_bind     1609..1623
                     /label=OL1270
     primer_bind     complement(1962..1996)
                     /label=OL1323
     primer_bind     1962..1996
                     /label=OL1324
     primer_bind     2093..2112
                     /label=OL1447
     primer_bind     complement(2296..2315)
                     /label=OL1446
     primer_bind     2521..2535
                     /label=OL1271
     primer_bind     2911..2930
                     /label=OL2835
     primer_bind     3018..3057
                     /label=OL4211
     primer_bind     3019..3053
                     /label=OL2384
     primer_bind     complement(3021..3053)
                     /label=OL1893
     primer_bind     3267..3281
                     /label=OL1272
     primer_bind     complement(3300..3331)
                     /label=OL1316
     primer_bind     3300..3331
                     /label=OL1317
     primer_bind     complement(3354..3373)
                     /label=OL1921
     primer_bind     3445..3461
                     /label=OL2390
     primer_bind     4374..4393
                     /label=OL2391
     primer_bind     complement(4404..4424)
                     /label=pEGFP-N3
     primer_bind     4604..4623
                     /label=OL1328
     primer_bind     complement(4875..4916)
                     /label=OL2388
     CDS             4902..4979
                     /label=SGGSx2-sv40NLS-SGGSx2
                     /note="/created_by=chenp"
     primer_bind     4902..4924
                     /label=OL1326
     primer_bind     complement(4940..4979)
                     /label=OL4201
     primer_bind     4941..4957
                     /label=OL1153
     primer_bind     complement(4949..4965)
                     /label=OL1154
     primer_bind     complement(4953..4980)
                     /label=OL1015
     primer_bind     complement(4955..5007)
                     /label=OL5276
     primer_bind     4958..4980
                     /label=OL1014
     CDS             4959..4979
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     4959..4979
                     /label=OL1313
     primer_bind     complement(4964..4980)
                     /label=OL1040
     primer_bind     4990..5044
                     /label=OL5274
     CDS             5028..5501
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP-7
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQ"
     primer_bind     5028..5048
                     /label=OL1686
     primer_bind     5028..5048
                     /label=OL1704
     primer_bind     5028..5046
                     /label=OL3080
     primer_bind     complement(5073..5093)
                     /label=pEGFP-N3
     primer_bind     complement(5082..5099)
                     /label=OL 4576
     primer_bind     complement(5249..5268)
                     /label=OL957
     primer_bind     5297..5316
                     /label=OL4348
     primer_bind     complement(5410..5428)
                     /label=OL3733
     primer_bind     5459..5481
                     /label=OL3306
     primer_bind     complement(5485..5519)
                     /label=OL5275
     primer_bind     complement(5502..5525)
                     /label=OL1314
     primer_bind     5502..5524
                     /label=OL5277
     primer_bind     5505..5534
                     /label=OL1014
     primer_bind     complement(5505..5534)
                     /label=OL900
     primer_bind     complement(5505..5531)
                     /label=OL1015
     primer_bind     complement(5505..5529)
                     /label=OL1040
     primer_bind     5505..5525
                     /label=OL4016
     primer_bind     5505..5525
                     /label=OL4323
     primer_bind     5505..5524
                     /label=OL1207
     primer_bind     5505..5519
                     /label=OL2537
     primer_bind     complement(5507..5524)
                     /label=OL2808
     primer_bind     5510..5533
                     /label=OL1035
     CDS             5510..5527
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     5510..5527
                     /label=OL1133
     primer_bind     complement(5510..5527)
                     /label=OL1152
     primer_bind     complement(5535..5554)
                     /label=OL4216
     primer_bind     5540..5555
                     /label=OL4334
     primer_bind     complement(5550..5567)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5556..5780
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal
                     牛生长激素聚腺苷酸化信号
                     牛生长激素（BGH）多聚腺苷酸化信号能够保证高效的转录终止和mRNA的poly A结构"
     primer_bind     5556..5575
                     /label=OL1407
     primer_bind     complement(5561..5580)
                     /label=OL1410
     primer_bind     complement(5600..5617)
                     /label=OL698
     primer_bind     complement(5601..5619)
                     /label=OL 4921
     primer_bind     complement(5602..5621)
                     /label=OL1938
     primer_bind     5661..5682
                     /label=OL 5131
     primer_bind     complement(5675..5700)
                     /label=OL 4990
     primer_bind     complement(5690..5710)
                     /label=OL4349
     primer_bind     complement(5728..5774)
                     /label=OL4452
     primer_bind     5747..5760
                     /label=OL4516
     primer_bind     complement(5750..5763)
                     /label=OL4335
     primer_bind     complement(5759..5779)
                     /label=OL4179
     primer_bind     complement(5761..5780)
                     /label=OL1684
     primer_bind     complement(5851..5867)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5851..5867)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5864..5886)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5875..5891
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5899..5929)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     5913..5932
                     /label=OL 3278
     protein_bind    5944..5965
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5948..5971)
                     /label=OL5162
     primer_bind     5957..5977
                     /label=OL5159
     primer_bind     complement(6082..6099)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6185..6212)
                     /label=OL 4634
     primer_bind     6192..6210
                     /label=OL 4970
     primer_bind     6192..6208
                     /label=OL3695
     primer_bind     6192..6208
                     /label=OL3920
     primer_bind     complement(6192..6208)
                     /label=OL3923
     primer_bind     complement(6192..6207)
                     /label=OL3924
     primer_bind     6209..6228
                     /label=OL4558
     primer_bind     6209..6223
                     /label=OL3984
     primer_bind     6227..6245
                     /label=OL 4593
     rep_origin      complement(6253..6841)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6292..6312)
                     /label=OL 4607
     primer_bind     complement(6333..6352)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6395..6413)
                     /label=OL4337
     primer_bind     complement(6435..6454)
                     /label=OL1041
     primer_bind     complement(6568..6587)
                     /label=OL631
     primer_bind     6624..6643
                     /label=OL798
     primer_bind     6885..6904
                     /label=OL659
     CDS             complement(7012..7872)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7308..7323)
                     /label=OL3925
     primer_bind     7325..7339
                     /label=OL3926
     primer_bind     7469..7488
                     /label=OL1922
     primer_bind     7635..7654
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(7635..7654)
                     /label=OL1923
     primer_bind     7788..7807
                     /label=OL1033
     primer_bind     complement(7788..7807)
                     /label=OL1034
     primer_bind     7870..7899
                     /label=OL 4897
     promoter        complement(7873..7977)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7951..7981
                     /label=OL 3277
     primer_bind     complement(7951..7980)
                     /label=OL 3274
     primer_bind     complement(8056..8075)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8056..8075
                     /label=OL1919
     primer_bind     complement(8059..8076)
                     /label=OL 4985
ORIGIN
        1 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
       61 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
      121 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      181 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      241 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      301 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      361 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      421 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      481 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      541 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      601 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      661 aagcagagct ggtttagtga accgtcagat ccgctagaga tccgcggccg ctaatacgac
      721 tcactatagg gagagccgcc accatgaaac ggacagccga cggaagcgag ttcgagtcac
      781 caaagaagaa gcggaaagtc gacaagaagt acagcatcgg cctggacatc ggcaccaact
      841 ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc
      901 tgggcaacac cgaccggcac agcatcaaga agaacctgat cggagccctg ctgttcgaca
      961 gcggcgaaac agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac
     1021 ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg
     1081 acagcttctt ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc
     1141 ggcaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca
     1201 tctaccacct gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct
     1261 atctggccct ggcccacatg atcaagttcc ggggccactt cctgatcgag ggcgacctga
     1321 accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc
     1381 tgttcgagga aaaccccatc aacgccagcg gcgtggacgc caaggccatc ctgtctgcca
     1441 gactgagcaa gagcagaaag ctggaaaatc tgatcgccca gctgcccggc gagaagaaga
     1501 atggcctgtt cggaaacctg attgccctga gcctgggcct gacccccaac ttcaagagca
     1561 acttcgacct ggccgaggat gccaaactgc agctgagcaa ggacacctac gacgacgacc
     1621 tggacaacct gctggcccag atcggcgacc agtacgccga cctgtttctg gccgccaaga
     1681 acctgtccga cgccatcctg ctgagcgaca tcctgagagt gaacaccgag atcaccaagg
     1741 cccccctgag cgcctctatg atcaagagat acgacgagca ccaccaggac ctgaccctgc
     1801 tgaaagctct cgtgcggcag cagctgcctg agaagtacaa agagattttc ttcgaccaga
     1861 gcaagaacgg ctacgccggc tacattgacg gcggagccag ccaggaagag ttctacaagt
     1921 tcatcaagcc catcctggaa aagatggacg gcaccgagga actgctcgtg aagctgaaga
     1981 gagaggacct gctgcggaag cagcggacct tcgacaacgg cagcatcccc caccagatcc
     2041 acctgggaga gctgcacgcc attctgcggc ggcaggaaga tttttaccca ttcctgaagg
     2101 acaaccggga aaagatcgag aagatcctga ccttccgcat cccctactac gtgggccctc
     2161 tggccagggg aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc
     2221 cctggaactt cgaggaagtg gtggacaagg gcgcttccgc ccagagcttc atcgagcgga
     2281 tgaccaactt cgataagaac ctgcccaacg agaaggtgct gcccaagcac agcctgctgt
     2341 acgagtactt caccgtgtat aacgagctga ccaaagtgaa atacgtgacc gagggaatga
     2401 gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga
     2461 ccaaccggaa agtgaccgtg aagcagctga aagaggacta cttcaagaaa atcgagtgct
     2521 tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg ggcacatacc
     2581 acgatctgct gaaaattatc aaggacaagg acttcctgga caatgaggaa aacgaggaca
     2641 ttctggaaga tatcgtgctg accctgacac tgtttgagga cagagagatg atcgaggaac
     2701 ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat gaagcagctg aagcggcgga
     2761 gatacaccgg ctggggcagg ctgagccgga agctgatcaa cggcatccgg gacaagcagt
     2821 ccggcaagac aatcctggat ttcctgaagt ccgacggctt cgccaacaga aacttcatgc
     2881 agctgatcca cgacgacagc ctgaccttta aagaggacat ccagaaagcc caggtgtccg
     2941 gccagggcga tagcctgcac gagcacattg ccaatctggc cggcagcccc gccattaaga
     3001 agggcatcct gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca
     3061 agcccgagaa catcgtgatc gaaatggcca gagagaacca gaccacccag aagggacaga
     3121 agaacagccg cgagagaatg aagcggatcg aagagggcat caaagagctg ggcagccaga
     3181 tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa cgagaagctg tacctgtact
     3241 acctgcagaa tgggcgggat atgtacgtgg accaggaact ggacatcaac cggctgtccg
     3301 actacgatgt ggacgctatc gtgcctcaga gctttctgaa ggacgactcc atcgacaaca
     3361 aggtgctgac cagaagcgac aagaaccggg gcaagagcga caacgtgccc tccgaagagg
     3421 tcgtgaagaa gatgaagaac tactggcggc agctgctgaa cgccaagctg attacccaga
     3481 gaaagttcga caatctgacc aaggccgaga gaggcggcct gagcgaactg gataaggccg
     3541 gcttcatcaa gagacagctg gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc
     3601 tggactcccg gatgaacact aagtacgacg agaatgacaa gctgatccgg gaagtgaaag
     3661 tgatcaccct gaagtccaag ctggtgtccg atttccggaa ggatttccag ttttacaaag
     3721 tgcgcgagat caacaactac caccacgccc acgacgccta cctgaacgcc gtcgtgggaa
     3781 ccgccctgat caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc gactacaagg
     3841 tgtacgacgt gcggaagatg atcgccaaga gcgagcagga aatcggcaag gctaccgcca
     3901 agtacttctt ctacagcaac atcatgaact ttttcaagac cgagattacc ctggccaacg
     3961 gcgagatccg gaagcggcct ctgatcgaga caaacggcga aaccggggag atcgtgtggg
     4021 ataagggccg ggattttgcc accgtgcgga aagtgctgag catgccccaa gtgaatatcg
     4081 tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga gtctatcctg cccaagagga
     4141 acagcgataa gctgatcgcc agaaagaagg actgggaccc taagaagtac ggcggcttcg
     4201 acagccccac cgtggcctat tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca
     4261 agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat catggaaaga agcagcttcg
     4321 agaagaatcc catcgacttt ctggaagcca agggctacaa agaagtgaaa aaggacctga
     4381 tcatcaagct gcctaagtac tccctgttcg agctggaaaa cggccggaag agaatgctgg
     4441 cctctgccgg cgaactgcag aagggaaacg aactggccct gccctccaaa tatgtgaact
     4501 tcctgtacct ggccagccac tatgagaagc tgaagggctc ccccgaggat aatgagcaga
     4561 aacagctgtt tgtggaacag cacaagcact acctggacga gatcatcgag cagatcagcg
     4621 agttctccaa gagagtgatc ctggccgacg ctaatctgga caaagtgctg tccgcctaca
     4681 acaagcaccg ggataagccc atcagagagc aggccgagaa tatcatccac ctgtttaccc
     4741 tgaccaatct gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga
     4801 ggtacaccag caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc
     4861 tgtacgagac acggatcgac ctgtctcagc tgggaggtga ctccggcgga agctctggtg
     4921 gcagcaagcg gaccgccgac ggctctgaat tcgagagccc taagaagaaa agaaaggtgt
     4981 ctggtggcgg aggttcaggc ggtggaggat ccggtggtgg cgggtcaatg gtgagcaagg
     5041 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     5101 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     5161 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     5221 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     5281 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     5341 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     5401 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     5461 actacaacag ccacaacgtc tatatcatgg ccgacaagca gtagccggtc atcatcacca
     5521 tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     5581 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     5641 ttcctaataa aatgagaaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     5701 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     5761 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct cgataccgtc
     5821 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     5881 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctagggtgc
     5941 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     6001 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6061 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6241 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6841 agaagatcct ttgatctttt ctacggggtc tgacactcag tggaacgaaa actcacgtta
     6901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6961 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     7021 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     7081 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     7141 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     7201 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     7261 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     7321 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     7381 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7441 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7501 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7561 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7621 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7681 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7741 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7801 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7861 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7921 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7981 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcgatctc ccgatcccct
     8041 agggtctact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     8101 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
//