pFC331

LOCUS       pFC331              16382 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  AMA1 plasmid with Aspergillus optimized Cas9 and argB selection 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    pFC331
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16382)
  AUTHORS   Nodvig CS, Nielsen JB, Kogle ME, Mortensen UH
  TITLE     A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi.
  JOURNAL   PLoS One. 2015 Jul 15;10(7):e0133085. doi: 
            10.1371/journal.pone.0133085. eCollection 2015.
  PUBMED    26177455
REFERENCE   2  (bases 1 to 16382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16382
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     622..641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(860..951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     993..1012
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1018..1037
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1244..1277
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      1590..6838
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     CDS             8010..12113
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             12117..12137
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(14882..14915)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(15385..15402)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(15385..15401)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15500..15517)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15671..16259)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15751..15770)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(16381..859)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcgctacat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta
     1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatcga
     1321 cgaagcagct gacggccagt gccaagctta acgcgtaccg ggcccagtat atgttccgca
     1381 gatgactgga gctctgccat acgtgccctc tcaagcacca tttgttccat ctacagagac
     1441 tagtcaccaa ctagtctatc aagactcaca gggtacattg ctgagaccaa ctgaccagag
     1501 gcagggtagc ggattgacgg ctccatctcc ttcacttaca aggtctattg aaagcccttt
     1561 agcatcacca agcggagaat agattgttaa gcttattttt tgtatactgt tttgtgatag
     1621 cacgaagttt ttccacggta tcttgttaaa aatatatatt tgtggcgggc ttacctacat
     1681 caaattaata agagactaat tataaactaa acacacaagc aagctacttt agggtaaaag
     1741 tttataaatg cttttgacgt ataaacgttg cttgtattta ttattacaat taaaggtgga
     1801 tagaaaacct agagactagt tagaaactaa tctcaggttt gcgttaaact aaatcagagc
     1861 ccgagaggtt aacagaacct agaaggggac tagatatccg ggtagggaaa caaaaaaaaa
     1921 aaacaagaca gccacatatt agggagacta gttagaagct agttccagga ctaggaaaat
     1981 aaaagacaat gataccacag tctagttgac aactagatag attctagatt gaggccaaag
     2041 tctctgagat ccaggttagt tgcaactaat actagttagt atctagtctc ctataactct
     2101 gaagctagaa taacttacta ctattatcct caccactgtt cagctgcgca aacggagtga
     2161 ttgcaaggtg ttcagagact agttattgac tagtcagtga ctagcaataa ctaacaaggt
     2221 attaacctac catgtctgcc atcaccctgc acttcctcgg gctcagcagc cttttcctcc
     2281 tcattttcat gctcattttc cttgtttaag actgtgacta gtcaaagact agtccagaac
     2341 cacaaaggag aaatgtctta ccactttctt cattgcttgt ctcttttgca ttatccatgt
     2401 ctgcaactag ttagagtcta gttagtgact agtccgacga ggacttgctt gtctccggat
     2461 tgttggagga actctccagg gcctcaagat ccacaacaga gccttctaga agactggtca
     2521 ataactagtt ggtctttgtc tgagtctgac ttacgaggtt gcatactcgc tccctttgcc
     2581 tcgtcaatcg atgagaaaaa gcgccaaaac tcgcaatatg gctttgaacc acacggtgct
     2641 gagactagtt agaatctagt cccaaactag cttggatagc ttacctttgc cctttgcgtt
     2701 gcgacaggtc ttgcagggta tggttccttt ctcaccagct gatttagctg ccttgctacc
     2761 ctcacggcgg atctgcataa agagtggcta gaggttataa attagcactg atcctaggta
     2821 cggggctgaa tgtaacttgc cyttcctttc tcatcgcgcg gcaagacagg cttgctcaaa
     2881 ttcctaccag tcacaggggt atgcacggcg tacggaccac ttgaactagt cacagattag
     2941 ttagcaacta gtctgcattg aatggctgta cttacgggcc ctcgccattg tcctgatcat
     3001 ttccagcttc accctcgttg ctgcaaagta gttagtgact agtcaaggac tagttgaaat
     3061 gggagaagaa actcacgaat tctcgacacc cttagtattg tggtccttgg acttggtgct
     3121 gctatatatt agctaataca ctagttagac tcacagaaac ttacgcagct cgcttgcgct
     3181 tcttggtagg agtcggggtt gggagaacag tgccttcaaa caagccttca taccatgcta
     3241 cttgactagt cagggactag tcaccaagta atctagatag gacttgcctt tggcctccat
     3301 cagttccttc atagtgggag gtccattgtg caatgtaaac tccatgccgt gggagttctt
     3361 gtccttcaag tgcttgacca atatgtttct gttggcagag ggaacctgtc aactagttaa
     3421 taactagtca gaaactagta tagcagtaga ctcactgtac gcttgaggca tcccttcact
     3481 cggcagtaga cttcatatgg atggatatca ggcacgccat tgtcgtcctg tggactagtc
     3541 agtaactagg cttaaagcta gtcgggtcgg cttactatct tgaaatccgg cagcgtaagc
     3601 tccccgtcct taactgcctc gagatagtga cagtactctg gggactttcg gagatcgtta
     3661 tcgcgaatgc tcggcatact aatcgttgac tagtcttgga ctagtcccga gcaaaaagga
     3721 ttggaggagg aggaggaagg tgagagtgag acaaagagcg aaataagagc ttcaaaggct
     3781 atctctaagc agtatgaagg ttaagtatct agttcttgac tagatttaaa agagatttcg
     3841 actagttatg tacctggagt ttggatatag gaatgtgttg tggtaacgaa atgtaaggga
     3901 gaggaaagaa aaagtcggtc aagaggtaac tctaagtcgg ccattccttt ttgggaggcg
     3961 ctaaccataa acggcatggt cgacttagag ttagctcagg gaatttaggg agttatctgc
     4021 gaccaccgag gaacggcgga atgccaaaga atcccgatgg agctctagct ggcggttgac
     4081 aaccccacct tttggcgttt ctgcggcgtt gcaggcggga ctggatactt cgtagaacca
     4141 gaaaggcaag gcagaacgcg ctcagcaaga gtgttggaag tgatagcatg atgtgccttg
     4201 ttaactaggt caaaatctgc agtatgcttg atgttatcca aagtgtgaga gaggaaggtc
     4261 caaacataca cgattgggag agggcctagg tataagagtt tttgagtaga acgcatgtga
     4321 gcccagccat ctcgaggaga ttaaacacgg gccggcattt gatggctatg ttagtacccc
     4381 aatggaaacg gtgagagtcc agtggtcgca gataactccc taaattccct gagctaactc
     4441 taagtcgacc atgccgttta tggttagcgc ctcccaaaaa ggaatggccg acttagagtt
     4501 acctcttgac cgactttttc tttcctcccc cttacatttc gttaccacaa cacattccta
     4561 tatccaaact ccaggtacat aactagtcga aatctctttt aaatctagtc aagaactaga
     4621 tacttaacct tcatactgct tagagatagc ctttgaagct cttatttcgc tctttgtctc
     4681 actctcacct tcctcctcct cctccaatcc tttttgctcg ggactagtcc aagactagtc
     4741 aacgattagt atgccgagca ttcgcgataa cgatctccga aagtccccag agtactgtca
     4801 ctatctcgag gcagttaagg acggggagct tacgctgccg gatttcaaga tagtaagccg
     4861 acccgactag ctttaagcct agttactgac tagtccacag gacgacaatg gcgtgcctga
     4921 tatccatcca tatgaagtct actgccgagt gaagggatgc ctcaagcgta cagtgagtct
     4981 actgctatac tagtttctga ctagttatta actagttgac aggttccctc tgccaacaga
     5041 aacatattgg tcaagcactt gaaggacaag aactcccacg gcatggagtt tacattgcac
     5101 aatggacctc ccactatgaa ggaactgatg gaggccaaag gcaagtccta tctagattac
     5161 ttggtgacta gtccctgact agtcaagtag catggtatga aggcttgttt gaaggcactg
     5221 ttctcccaac cccgactcct accaagaagc gcaagcgagc tgcgtaagtt tctgtgagtc
     5281 taactagtgt attagctaat atatagcagc accaagtcca aggaccacaa tactaagggt
     5341 gtcgagaatt cgtgagtttc ttctcccatt tcaactagtc cttgactagt cactaactac
     5401 tttgcagcaa cgagggtgaa gctggaaatg atcaggacaa tggcgagggc ccgtaagtac
     5461 agccattcaa tgcagactag ttgctaacta atctgtgact agttcaagtg gtccgtacgc
     5521 cgtgcatacc cctgtgactg gtaggaattt gagcaagcct gtcttgccgc gcgatgagaa
     5581 aggaarggca agttacattc agccccgtac ctaggatcag tgctaattta taacctctag
     5641 ccactcttta tgcagatccg ccgtgagggt agcaaggcag ctaaatcagc tggtgagaaa
     5701 ggaaccatac cctgcaagac ctgtcgcaac gcaaagggca aaggtaagct atccaagcta
     5761 gtttgggact agattctaac tagtctcagc accgtgtggt tcaaagccat attgcgagtt
     5821 ttggcgcttt ttctcatcga ttgacgaggc aaagggagcg agtatgcaac ctcgtaagtc
     5881 agactcagac aaagaccaac tagttattga ccagtcttct agaaggctct gttgtggatc
     5941 ttgaggccct ggagagttcc tccaacaatc cggagacaag caagtcctcg tcggactagt
     6001 cactaactag actctaacta gttgcagaca tggataatgc aaaagagaca agcaatgaag
     6061 aaagtggtaa gacatttctc ctttgtggtt ctggactagt ctttgactag tcacagtctt
     6121 aaacaaggaa aatgagcatg aaaatgagga ggaaaaggct gctgagcccg aggaagtgca
     6181 gggtgatggc agacatggta ggttaatacc ttgttagtta ttgctagtca ctgactagtc
     6241 aataactagt ctctgaacac cttgcaatca ctccgtttgc gcagctgaac agtggtgagg
     6301 ataatagtag taagttattc tagcttcaga gttataggag actagatact aactagtatt
     6361 agttgcaact aacctggatc tcagagactt tggcctcaat ctagaatcta tctagttgtc
     6421 aactagactg tggtatcatt gtcttttatt ttcctagtcc tggaactagc ttctaactag
     6481 tctccctaat atgtggctgt cttgtttttt ttttttgttt ccctacccgg atatctagtc
     6541 cccttctagg ttctgttaac ctctcgggct ctgatttagt ttaacgcaaa cctgagatta
     6601 gtttctaact agtctctagg ttttctatcc acctttaatt gtaataataa atacaagcaa
     6661 cgtttatacg tcaaaagcat ttataaactt ttaccctaaa gtagcttgct tgtgtgttta
     6721 gtttataatt agtctcttat taatttgatg taggtaagcc cgccacaaat atatattttt
     6781 aacaagatac cgtggaaaaa cttcgtgcta tcacaaaaca gtatacaaaa aataagctta
     6841 acaatctatt ctccgcttgg tgatgctaaa gggctttcaa tagaccttgt aagtgaagga
     6901 gatggagccg tcaatccgct accctgcctc tggtcagttg gtctcagcaa tgtaccctgt
     6961 gagtcttgat agactagttg gtgactagtc tctgtagatg gaacaaatgg tgcttgagag
     7021 ggcacgtatg gcagagctcc agtcatctgc ggaacatata ctgggcccgg gaagatctca
     7081 tggtcatagc tgtttccgct gagggtttaa ttaagacctc agccgagaca gcagaatcac
     7141 cgcccaagtt aagcctttgt gctgatcatg ctctcgaacg ggccaagttc gggaaaagca
     7201 aaggagcgtt tagtgagggg caatttgact cacctcccag gcaacagatg aggggggcaa
     7261 aaagaaagaa attttcgtga gtcaatatgg attccgagca tcattttctt gcggtctatc
     7321 ttgctacgta tgttgatctt gacgctgtgg atcaagcaac gccactcgct cgctccatcg
     7381 caggctggtc gcagacaaat taaaaggcgg caaactcgta cagccgcggg gttgtccgct
     7441 gcaaagtaca gagtgataaa agccgccatg cgaccatcaa cgcgttgatg cccagctttt
     7501 tcgatccgag aatccaccgt agaggcgata gcaagtaaag aaaagctaaa caaaaaaaaa
     7561 tttctgcccc taagccatga aaacgagatg gggtggagca gaaccaagga aagagtcgcg
     7621 ctgggctgcc gttccggaag gtgttgtaaa ggctcgacgc ccaaggtggg agtctaggag
     7681 aagaatttgc atcgggagtg gggcgggtta cccctccata tccaatgaca gatatctacc
     7741 agccaagggt ttgagcccgc ccgcttagtc gtcgtcctcg cttgcccctc cataaaagga
     7801 tttcccctcc ccctcccaca aaattttctt tcccttcctc tccttgtccg cttcagtacg
     7861 tatatcttcc cttccctcgc ttctctcctc catccttctt tcatccatct cctgctaact
     7921 tctctgctca gcacctctac gcattactag ccgtagtatc tgagcacttc tcccttttat
     7981 attccacaaa acataacaca accttcacca tggacaagaa gtatagcatc gggctggaca
     8041 ttggaacgaa ctcggttggt tgggctgtga ttacggacga atacaaggtg ccatccaaga
     8101 agtttaaggt cctgggaaac accgaccgtc actcaatcaa gaagaatctc attggagccc
     8161 tgctcttcga tagtggggag accgccgaag ctactcgact gaagcgaacg gctcgccggc
     8221 gttatacacg acgcaagaat cgcatctgct acctccagga gattttcagc aacgaaatgg
     8281 ctaaggttga tgactcattc tttcatcgac tcgaagaaag tttcttggtc gaggaggata
     8341 agaagcacga gcgccatccg atctttggta acattgtgga tgaggttgcc tatcacgaaa
     8401 agtacccaac tatctatcat cttcgtaaga agctggtcga tagcacggac aaggctgatt
     8461 tgcgacttat ctacctggca ctcgcgcaca tgattaagtt ccgcggccat tttcttatcg
     8521 agggtgacct gaaccccgat aattctgacg ttgataagct cttcatccag ttggtccaaa
     8581 cctacaatca gctgtttgag gaaaacccta ttaatgcatc tggcgtggac gccaaggcta
     8641 tcctttcggc gcgcctgtct aagtcgcggc gtttggagaa ccttatcgca caactccccg
     8701 gcgaaaagaa gaacggcctc ttcggtaatt tgattgcgtt gtcacttggt ctgactccta
     8761 acttcaagag taattttgac ctggcagagg atgcgaagct ccagttgtct aaggatacgt
     8821 atgatgacga tctcgacaac ttgcttgccc aaatcggtga ccagtacgct gatcttttcc
     8881 tggccgctaa gaatctctca gatgcaatcc tgctcagtga cattttgcgg gtcaacaccg
     8941 agattactaa ggcccccctg tcagctagta tgatcaagcg gtatgatgag caccatcagg
     9001 acctcacctt gcttaaggcc ctcgtgcgtc agcaattgcc tgagaagtat aaggaaatct
     9061 tctttgacca atccaagaac ggatacgcag ggtatattga tggcggtgcg agccaggagg
     9121 aattctacaa gtttatcaag ccgattttgg agaagatgga cggcactgag gaactgctcg
     9181 tcaagctgaa tcgcgaagat ttgcttcgta agcaacgaac gttcgacaac ggctccatcc
     9241 cgcaccagat tcatctgggc gagctccacg ccatccttcg acgccaggaa gatttctacc
     9301 catttctgaa ggacaaccgt gagaagatcg aaaagattct tacattccga atcccctact
     9361 atgtgggacc tttggcccgt gggaattccc gatttgcttg gatgacccga aagagcgagg
     9421 aaaccatcac tccgtggaac ttcgaggaag tcgtggacaa gggtgcatcc gcgcagagct
     9481 tcattgagcg gatgaccaat tttgataaga accttccgaa tgaaaaggtc ctgccaaagc
     9541 attcgctgct ctacgagtat ttcaccgtgt ataacgaact gactaaggtc aagtacgtga
     9601 cggagggaat gcggaagcca gccttcctct caggggaaca aaagaaggct atcgtcgatt
     9661 tgctttttaa gaccaatcgt aaagtgactg ttaagcagct gaaggaggat tatttcaaga
     9721 agattgaatg tttcgactcc gtcgagatca gcggcgtgga agatcgcttt aacgcttccc
     9781 tcggtaccta ccacgacctg ctcaagatca ttaaggacaa ggatttcctc gataacgagg
     9841 aaaatgagga catcttggaa gatattgtcc tcacgttgac actttttgag gaccgcgaaa
     9901 tgatcgagga acggctcaag acatatgccc atttgttcga cgataaggtg atgaagcagc
     9961 tgaagcggcg tcgatacacc ggatggggtc gccttagccg gaagctgatc aacggcattc
    10021 gagataagca atctggtaag actatcttgg atttccttaa gtcggacggc ttcgccaacc
    10081 gcaattttat gcagcttatt cacgacgatt ccctgacgtt caaggaggac atccagaagg
    10141 cacaagtctc aggacaaggg gattccctgc acgagcatat cgccaacctg gctggatccc
    10201 cggcgatcaa gaaggggatt cttcagaccg tcaaggttgt cgacgagctg gtcaaggtga
    10261 tgggccgtca taagccagaa aacatcgtga ttgagatggc ccgagaaaat cagaccactc
    10321 aaaagggtca gaagaacagc cgcgagcgga tgaagcggat cgaggaaggc attaaggaac
    10381 ttggttctca gatcctgaag gagcaccctg ttgaaaacac acagctccaa aatgagaagc
    10441 tgtatctcta ctatttgcaa aatggacgcg acatgtacgt cgatcaggag ctcgacatta
    10501 accggttgtc ggactacgat gttgaccata tcgtcccgca atccttcctt aaggacgata
    10561 gcattgataa caaggtgctg actcgctcag ataagaaccg gggcaagtcc gacaatgttc
    10621 caagcgagga agtggttaag aagatgaaga actactggcg ccaattgctt aatgccaagc
    10681 tcatcacaca gcgcaagttt gacaacttga ccaaggccga gcggggaggg ctgagtgaac
    10741 tcgataaggc tggcttcatc aagcgtcaac tcgtggagac gcgacagatc acaaagcacg
    10801 ttgctcagat tctggactcc cggatgaaca caaagtacga cgagaatgat aagctcatcc
    10861 gtgaagttaa ggtcattacc ctcaagtcta agttggtgtc ggatttccgc aaggacttcc
    10921 aattttataa ggttcgggag atcaacaatt atcaccatgc acatgatgcg tacctcaacg
    10981 cagtcgtggg aactgcgctc atcaagaagt atcccaagtt ggagtccgaa ttcgtctacg
    11041 gggattataa ggtttacgac gtccgcaaga tgatcgccaa gagtgagcag gaaattggca
    11101 aggccacggc taagtatttc ttttactcca acatcatgaa tttctttaag acggagatca
    11161 cactcgccaa tggagaaatc cgtaagcgac ctttgattga gaccaacggc gagactggtg
    11221 aaatcgtttg ggataagggg cgcgacttcg ctaccgtgcg gaaggttctg agcatgccgc
    11281 aagtcaatat cgtcaagaaa accgaggtgc agacaggcgg tttctctaag gaatcgattc
    11341 ttccaaagcg taactctgac aagctgatcg ctcgaaagaa ggattgggac cccaagaagt
    11401 atggagggtt cgattctcct acagtggcat actcggttct cgttgtcgcg aaggttgaga
    11461 agggaaagtc taagaagctg aagtcggtca aggaactgct cgggatcacc attatggagc
    11521 gctccagctt cgaaaagaat cccatcgact ttctcgaggc caagggctat aaggaagtca
    11581 agaaggatct tatcattaag ctgcctaagt actctttgtt cgagcttgaa aacggtcgaa
    11641 agcgaatgct cgcatcggca ggagagttgc agaaggggaa tgaattggca cttccctcaa
    11701 agtacgtgaa cttcctgtat ctcgcgtccc actacgagaa gctgaagggt agccctgagg
    11761 acaacgaaca gaagcaactt tttgttgagc aacacaagca ttatctggat gagatcattg
    11821 aacagatttc agagttcagt aagcgcgtca tcctcgccga tgctaatctc gacaaggtgt
    11881 tgtcggccta caacaagcac cgtgacaagc cgatccgaga gcaggctgaa aatatcattc
    11941 atctgttcac cctcactaac ttgggagcac cagcagcgtt caagtatttt gatacgacaa
    12001 tcgaccgtaa gcgatacacg tccacaaagg aggtgcttga tgcgaccctg attcatcaat
    12061 ccatcactgg gctctatgaa acccgtatcg accttagtca actggggggc gaccctccca
    12121 agaagaagcg caaggtctga gcggacattc gatttatgcc gttatgactt ccttaaaaaa
    12181 gcctttacga atgaaagaaa tggaattaga cttgttatgt agttgattct acaatggatt
    12241 atgattcctg aacttcaaat ccgctgttca ttattaatct cagctcttcc cgtaaagcca
    12301 atgttgaaac tattcgtaaa tgtacctcgt tttgcgtgta ccttgcttat cacgtgatat
    12361 tacatgacct ggacagagtt ctgcgcgaaa gtcataacgt aaatcccggg cggtaggtgc
    12421 gtcccgggcg gaaggtagtt ttctcgtcca ccccaacgcg tttatcaacc tcaactttca
    12481 acaaccatca tgccaccaaa agcgcgtaaa acaaagcgag atttgattga gcaagagggc
    12541 aggatccaat gcgcgattca agacattaaa aatggaaaat ttcaaaaaat tgcgcccgca
    12601 gcgcgtgcat acaaaattca tcccaataca agtgtaatac taggtaatat cgcgtgcatt
    12661 ccgcggtttt ttggggtagt catctaatga aacagacccg gacgcagcag aggaagcccg
    12721 cgatgactct ataccaccgt acgccgatat atcatcatcg cggcgatgga gaagttgggt
    12781 tgactccgaa gacacttcaa aggagcgacg ctgttgattt gtagacgacg cttgataggg
    12841 agaagcatta ttgtcgtgat gctcgcccaa cagaggccga ctcgcctcat ccgtcataac
    12901 gaacgctgtg taaagcggag tgggggggaa agtgtggatt gtggagagta tgcgatagtg
    12961 ttgaggctga tcagacggcg aatcgggcca gatatgacca gtttagaggc ctcatttgac
    13021 tataatttac ataaattaga taaatagaga tgaacgcatg caataattgc agcaaatatt
    13081 gatgaagcga gaggtaggac gatgaaggac tgtgagcagt tcaaggtatc agcagagtca
    13141 agggcctgat gcaatggcgg tgatccgtga tcagcgaacg gaaggggcgc taactctgtt
    13201 tctttaccaa tgatcggaag ctcctgctgg cggacttatg agtcattcac gaatcatttc
    13261 tcagttattt gtggatgccc tcgttctgtc cacaatttct ttccgcccca agtcttttaa
    13321 gttctttaac atctatattc ttgcacttcc aatggcatcc cttcgctccg tactcaagag
    13381 ccagagcttg cgacacaccg tgcgatccta ctcctcgcaa accatgcctc ccgcctcacc
    13441 ctttgctccc cgccacttcc tctccattgc ggacctctcg ccctccgagt tcgcaaccct
    13501 tgttcgcaat gcctcctcac acaaacgggc tatcaagtcg gggtcaatgc cccaaaactt
    13561 gcagggatca ctccttggga aaactgtggc catgatcttc agcaaacgaa gcacgaggac
    13621 aagggtatct acagaagggg ccgttgtgca gatgggaggt catccgatgt tcttgggcaa
    13681 ggatgatatc caactaggtg tcaacgagtc cctatacgac acctccgttg tcatttcgtc
    13741 catggtatcc tgcattgtag cccgtgtcgg taaacatgca gaggtcgcag atctggcgaa
    13801 gcactcttcg gttccagtca tcaatgcttt gtgtgactct ttccaccctc tccaagccgt
    13861 ggccgatttc cagaccatct atgaagcatt cacccccaag gcgcaccacc tttcaagtct
    13921 agggttggaa ggattgaaga tcgcttgggt gggtgacgcc aacaacgtcc tgttcgatat
    13981 ggccattgct gctacaaaaa tgggtgtcga cattgctgtc gctactccca aggggtacga
    14041 aatccctcct cacatgctgg agctcatcaa gtctgctgga gagggtgtct cgaaaccagg
    14101 aaagcttctg caaaccaata ttcccgaaga agcggtcaag gacgccgata ttctggtcac
    14161 agacacctgg gtctctatgg gccaagagga agagaaggct cagaggctga aggagtttga
    14221 tggtttccaa atcactgctg aactcgccaa gcgaggagga gctaaggagg gctggaagtt
    14281 catgcactgt ctcccgcgac accctgagga ggtcagcgac gaggttttct acagcaaccg
    14341 gtcacttgtc ttccctgagg ctgagaaccg gttatgggct gcgatttccg ccttggaggg
    14401 tttcgttgtc aataagggaa aaattgaata aatataacca ggcttccatt taaatatata
    14461 gaggctggcg ttatcaaact gatgagttga cgggtatgag atcattccgt ccctaaatat
    14521 atttactccg atcacgtaaa agcctgttag tagaagcatt ttcccaatta tcctgaccaa
    14581 ttctttctag catatatcaa ataactaatt gacatgttct ctcgcttcct tatattactc
    14641 agagtattgg aaatggggca aatcgcaccc ggtgactttc acatgtcacg aatgcggagt
    14701 cgtcctagcc aaggtagatc caggcctaac acaccccaac cctcgactct cctcattcca
    14761 tactcttgac ctctatccag cacattcttc tgaggtttcg caatggctgt aggtggcgct
    14821 gaggcatccc cactaccgca ttaagacctc agcgcggccg caaatttaaa taaaatgaag
    14881 tgaagttcct atactttcta gagaatagga acttctatag tgagtcgaat aagggcgaca
    14941 caaaatttat tctaaatgca taataaatac tgataacatc ttatagtttg tattatattt
    15001 tgtattatcg ttgacatgta taattttgat atcaaaaact gattttccct ttattatttt
    15061 cgagatttat tttcttaatt ctctttaaca aactagaaat attgtatata caaaaaatca
    15121 taaataatag atgaatagtt taattatagg tgttcatcaa tcgaaaaagc aacgtatctt
    15181 atttaaagtg cgttgctttt ttctcattta taaggttaaa taattctcat atatcaagca
    15241 aagtgacagg cgcccttaaa tattctgaca aatgctcttt ccctaaactc cccccataaa
    15301 aaaacccgcc gaagcgggtt tttacgttat ttgcggatta acgattactc gttatcagaa
    15361 ccgcccaggg ggcccgagct taagactggc cgtcgtttta caacacagaa agagtttgta
    15421 gaaacgcaaa aaggccatcc gtcaggggcc ttctgcttag tttgatgcct ggcagttccc
    15481 tactctcgcc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    15541 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    15601 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    15661 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    15721 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    15781 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    15841 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    15901 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    15961 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    16021 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    16081 agtggtgggc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
    16141 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    16201 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    16261 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgacgcg cgcgtaactc
    16321 acgttaaggg attttggtca tgagcttgcg ccgtcccgtc aagtcagcgt aatgctctgc
    16381 tt
//