psiCHECK-2-h1-cluster15

LOCUS       psiCHECK-2-h1-cluster15            6660 bp DNA     circular SYN 16-MAY-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Sep 15, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..1629
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    1681..2059
                     /label=h1-cluster15
     polyA_signal    2075..2123
                     /note="synthetic polyadenylation signal"
     promoter        2132..2883
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      2913..2922
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2919..4571
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             3878..3886
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    4615..4736
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4869..4973
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4974..5834
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6005..6593
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag
      721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg
      781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt
      841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc
      901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat
      961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac
     1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac
     1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg
     1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa
     1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag
     1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag
     1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc
     1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat
     1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga
     1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag
     1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac
     1621 gagcagtaat tctaggcgat cgctcgagcc cgggaattcg tttaaaccta gagcggccgc
     1681 cccctgctat cttggttgct tcctgttctc acagaaatgt gaatgatgtc cctcatgtat
     1741 tgcttatttc ttgagctttt aggtacaact tggcaaatat accaaatgat agagaggaaa
     1801 aatgaaggta tttttacatt gaaaattttt cactggcaaa gctcttttcc tgaaggaaac
     1861 atcactcatg tttaggctag attatgtgcg catcgtgacc tagaattgga aacccagtgg
     1921 ctactgaact gggggctcct ctaaagccaa accaaaccat ttgagcaaga actatggact
     1981 attcatacag tttatcaaat ttagccagat gacagagaca gtgtctgaca aaaggttgaa
     2041 tctagtaaaa aaaaaaaaag cggccgctgg ccgcaataaa atatctttat tttcattaca
     2101 tctgtgtgtt ggttttttgt gtgaggatct aaatgagtct tcggacctcg cgggggccgc
     2161 ttaagcggtg gttagggttt gtctgacgcg gggggagggg gaaggaacga aacactctca
     2221 ttcggaggcg gctcggggtt tggtcttggt ggccacgggc acgcagaaga gcgccgcgat
     2281 cctcttaagc acccccccgc cctccgtgga ggcgggggtt tggtcggcgg gtggtaactg
     2341 gcgggccgct gactcgggcg ggtcgcgcgc cccagagtgt gaccttttcg gtctgctcgc
     2401 agacccccgg gcggcgccgc cgcggcggcg acgggctcgc tgggtcctag gctccatggg
     2461 gaccgtatac gtggacaggc tctggagcat ccgcacgact gcggtgatat taccggagac
     2521 cttctgcggg acgagccggg tcacgcggct gacgcggagc gtccgttggg cgacaaacac
     2581 caggacgggg cacaggtaca ctatcttgtc acccggaggc gcgagggact gcaggagctt
     2641 cagggagtgg cgcagctgct tcatccccgt ggcccgttgc tcgcgtttgc tggcggtgtc
     2701 cccggaagaa atatatttgc atgtctttag ttctatgatg acacaaaccc cgcccagcgt
     2761 cttgtcattg gcgaattcga acacgcagat gcagtcgggg cggcgcggtc ccaggtccac
     2821 ttcgcatatt aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca gcgacccgct
     2881 taaaagcttg gcattccggt actgttggta aagccaccat ggccgatgct aagaacatta
     2941 agaagggccc tgctcccttc taccctctgg aggatggcac cgctggcgag cagctgcaca
     3001 aggccatgaa gaggtatgcc ctggtgcctg gcaccattgc cttcaccgat gcccacattg
     3061 aggtggacat cacctatgcc gagtacttcg agatgtctgt gcgcctggcc gaggccatga
     3121 agaggtacgg cctgaacacc aaccaccgca tcgtggtgtg ctctgagaac tctctgcagt
     3181 tcttcatgcc agtgctgggc gccctgttca tcggagtggc cgtggcccct gctaacgaca
     3241 tttacaacga gcgcgagctg ctgaacagca tgggcatttc tcagcctacc gtggtgttcg
     3301 tgtctaagaa gggcctgcag aagatcctga acgtgcagaa gaagctgcct atcatccaga
     3361 agatcatcat catggactct aagaccgact accagggctt ccagagcatg tacacattcg
     3421 tgacatctca tctgcctcct ggcttcaacg agtacgactt cgtgccagag tctttcgaca
     3481 gggacaaaac cattgccctg atcatgaaca gctctgggtc taccggcctg cctaagggcg
     3541 tggccctgcc tcatcgcacc gcctgtgtgc gcttctctca cgcccgcgac cctattttcg
     3601 gcaaccagat catccccgac accgctattc tgagcgtggt gccattccac cacggcttcg
     3661 gcatgttcac caccctgggc tacctgattt gcggctttcg ggtggtgctg atgtaccgct
     3721 tcgaggagga gctgttcctg cgcagcctgc aagactacaa aattcagtct gccctgctgg
     3781 tgccaaccct gttcagcttc ttcgctaaga gcaccctgat cgacaagtac gacctgtcta
     3841 acctgcacga gattgcctct ggcggcgccc cactgtctaa ggaggtgggc gaagccgtgg
     3901 ccaagcgctt tcatctgcca ggcatccgcc agggctacgg cctgaccgag acaaccagcg
     3961 ccattctgat taccccagag ggcgacgaca agcctggcgc cgtgggcaag gtggtgccat
     4021 tcttcgaggc caaggtggtg gacctggaca ccggcaagac cctgggagtg aaccagcgcg
     4081 gcgagctgtg tgtgcgcggc cctatgatta tgtccggcta cgtgaataac cctgaggcca
     4141 caaacgccct gatcgacaag gacggctggc tgcactctgg cgacattgcc tactgggacg
     4201 aggacgagca cttcttcatc gtggaccgcc tgaagtctct gatcaagtac aagggctacc
     4261 aggtggcccc agccgagctg gagtctatcc tgctgcagca ccctaacatt ttcgacgccg
     4321 gagtggccgg cctgcccgac gacgatgccg gcgagctgcc tgccgccgtc gtcgtgctgg
     4381 aacacggcaa gaccatgacc gagaaggaga tcgtggacta tgtggccagc caggtgacaa
     4441 ccgccaagaa gctgcgcggc ggagtggtgt tcgtggacga ggtgcccaag ggcctgaccg
     4501 gcaagctgga cgcccgcaag atccgcgaga tcctgatcaa ggctaagaaa ggcggcaaga
     4561 tcgccgtgta ataattctag agtcggggcg gccggccgct tcgagcagac atgataagat
     4621 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     4681 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     4741 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     4801 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccaggtggc acttttcggg
     4861 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4921 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4981 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5041 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5101 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5161 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     5221 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5281 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5341 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5401 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5461 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5521 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5581 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5641 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5701 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5761 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5821 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5881 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5941 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6001 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6061 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6121 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6181 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6241 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6301 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6361 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6421 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6481 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6541 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6601 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atggctcgac
//