| 质粒名称: | pET28a_T7-ARG1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 106473 |
| 存储实验室: | Mikhail Shapiro |
|
载体骨架基本信息 |
|
| 载体名称: | pET28a |
| 载体出品公司: | Novagen |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 5374 bp |
| 完整质粒大小: | 12129 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | ARG1,Acoustic Reporter Gene 1,Hybrid Gas Vesicle gene cluster |
| 物种来源: | Anabaena flos-aquae and Bacillus megaterium |
| 插入大小: | 6755 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | T7 |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | T7_Forward |
| 3' 测序引物 | T7 terminator_Reverse |
|
文献引用方法 |
|
| 材料和方法部分: | pET28a_T7-ARG1 was a gift from Mikhail Shapiro (Addgene plasmid # 106473 ; http://n2t.net/addgene:106473 ; RRID:Addgene_106473) |
| 参考文献部分: | Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts. Bourdeau RW, Lee-Gosselin A, Lakshmanan A, Farhadi A, Kumar SR, Nety SP, Shapiro MG. Nature. 2018 Jan 3;553(7686):86-90. doi: 10.1038/nature25021. 10.1038/nature25021 PubMed 29300010 |
买家导航
pET28a_T7-ARG1载体质粒基本信息
pET28a_T7-ARG1质粒图谱载体图谱和pET28a_T7-ARG1载体序列质粒序列多克隆位点信息
pET28a_T7-ARG1质粒载体简介
pET28a_T7-ARG1质粒序列载体序列
LOCUS pET28a_T7-ARG1 12125 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses acoustic reporter genes or gas vesicles (ARG1) in
Escherichia coli.
ACCESSION .
VERSION .
KEYWORDS pET28a_T7-ARG1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12125)
AUTHORS Bourdeau RW, Lee-Gosselin A, Lakshmanan A, Farhadi A, Kumar SR, Nety
SP, Shapiro MG
TITLE Acoustic reporter genes for noninvasive imaging of microorganisms in
mammalian hosts.
JOURNAL Nature. 2018 Jan 3;553(7686):86-90. doi: 10.1038/nature25021.
PUBMED 29300010
REFERENCE 2 (bases 1 to 12125)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12125
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 12..467
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(99..118)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 309..330
/label=F1ori-F
/note="F1 origin, forward primer"
CDS complement(559..1374)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 1282..1301
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 1366..1385
/label=pENTR-R
/note="pENTR vectors, reverse primer"
rep_origin 1496..2084
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1985..2004
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2238..2255
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 2270..2409
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(2335..2354)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2495..2517
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(2511..2702)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(3511..4593)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 4555..4574
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(4594..4671)
/gene="lacI"
/label=lacI promoter
primer_bind 4877..4896
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind 4980..4999
/label=T7
/note="T7 promoter, forward primer"
promoter 4980..4998
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 4999..5023
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 8305..8322
/codon_start=1
/product="N-myristoylation signal from Src kinase (Pellman
et al., 1985; Kaplan et al., 1988)"
/label=myr
/translation="GSSKSK"
primer_bind complement(12039..12057)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 12053..12100
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
ORIGIN
1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
421 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
541 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
601 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
661 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
721 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
781 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
841 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
901 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
961 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
1021 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
1081 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
1141 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
1201 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
1261 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
1321 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc
1381 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac
1441 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
1501 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
1561 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
1621 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
1681 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
1741 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
1801 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
1861 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
1921 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
1981 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
2041 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
2101 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
2161 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
2221 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
2281 attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat
2341 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
2401 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
2461 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
2521 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
2581 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
2641 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
2701 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
2761 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
2821 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
2881 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
2941 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
3001 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
3061 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
3121 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg
3181 ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct
3241 tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc
3301 cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt
3361 tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg
3421 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga
3481 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
3541 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
3601 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
3661 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
3721 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc
3781 cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
3841 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
3901 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
3961 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
4021 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
4081 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc
4141 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
4201 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
4261 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc
4321 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc
4381 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
4441 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
4501 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
4561 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc
4621 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc
4681 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
4741 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
4801 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
4861 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
4921 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
4981 aatacgactc actatagggg aattgtgagc ggataacaat tccaagcttc gccagggttt
5041 tcccagtcga gctcatgact catatttata gttattctcc tcatttccca agtttccgac
5101 aacaaataac aaggaattaa aaaaatggca gttgaaaaaa ccaattcttc ctccagcttg
5161 gcagaagtta ttgatagaat cctcgacaaa ggtatcgtaa ttgacgcttg ggttcgtgtt
5221 tccttagttg gtatcgaact actagcaatt gaagctcgga tcgttatcgc ttccgttgaa
5281 acctacttga agtatgctga agcagttggt ttgacccaat cagcagcagt acctgcttaa
5341 tttaattaag caagttacca agtaggagaa taaccatgac tcatatttat agttattctc
5401 ctcatttccc aagtttccga caacaaataa caaggaatta aaaaaatggc agttgaaaaa
5461 accaattctt cctccagctt ggcagaagtt attgatagaa tcctcgacaa aggtatcgta
5521 attgacgctt gggttcgtgt ttccttagtt ggtatcgaac tactagcaat tgaagctcgg
5581 atcgttatcg cttccgttga aacctacttg aagtatgctg aagcagttgg tttgacccaa
5641 tcagcagcag tacctgctta atttaattaa gctccataat atcaggatta attaatcctg
5701 atattggttc tgcttttttt aattatctag atatttggaa atcaacgctt ctcaaaggat
5761 attacaaaaa agtacgaatc cttataatgt agtacctgga gaagcaattt ctgttaggtc
5821 tgggagctcc catgggatcg ttatcccgcg gccaagcttg ctagcgctaa tttactattt
5881 tttggagaac ttcatgattt ctttaatggc aaaaatccgg caagaacatc agtcaatagc
5941 agagaaagtg gctgaactat ctcttgagac cagagaattc ttgtccgtca cgacagcgaa
6001 aagacaagag caagctgaaa aacaagctca agaactgcaa gcattctaca aggatcttca
6061 ggaaacaagt cagcagtttt tatcagaaac agcccaagcc agaattgctc aagctgaaaa
6121 acaagctcaa gaactgttag cattccacaa agaacttcaa gaaacaagtc agcagttttt
6181 atcagcaaca gcccaagcca gaattgctca agctgaaaaa caagcgcaag aactgttagc
6241 attttatcaa gaagttcggg aaacaagtca gcagttttta tcagcaacag cccaagcaag
6301 aattgctcaa gctgaaaaac aagctcaaga actgttagca ttccacaaag aacttcaaga
6361 aacaagtcag cagtttttat cagcaacagc cgacgcaaga actgctcaag ctaaggaaca
6421 gaaggaatct ctcctgaaat tccgtcagga tttgtttgtg agtatctttg gttaataaag
6481 atctaactat tggaggctac taaaaatgga aattaaaaaa attatgcaag ccgtgaacga
6541 ctttttcggt gaacacgtag ctcctcctca taaaattacc tcggtggaag ctactgaaga
6601 tgaaggttgg agagttattg ttgaagtcat tgaagaacga gaatatatga aaaaatacgc
6661 caaagatgaa atgctcggaa cgtacgagtg ctttgtaaat aaagaaaaag aagtcatttc
6721 attcaaacga ctcgacgtca gatatagaag cgccattggc attgaagcat aaacgataag
6781 atggcaggag gaacgtaaaa atgaccgtct taacagacaa aaggaaaaaa ggcagtggag
6841 cttttataca agatgacgag acaaaagagg ttctttcaag agcgctgagc tatttaaaat
6901 ccggctattc cattcatttt acaggtcctg ccggcggagg caaaacctct ttagcgcgag
6961 cgcttgctaa aaagagaaag cgtcctgtaa tgctgatgca cgggaatcac gagctcaaca
7021 acaaagattt aattggcgat tttacgggat acacgagcaa aaaagtaatc gaccagtacg
7081 ttcgttctgt ctataaaaaa gatgaacagg tgagtgaaaa ctggcaggat ggccgattgc
7141 ttgaagctgt aaaaaatggc tatacgctga tttacgacga atttactcgt tctaagcctg
7201 cgacgaataa tatctttcta tcgatattag aagaaggcgt gctgccgctg tatggagtaa
7261 aaatgaccga tccttttgtg cgcgtgcatc ccgatttccg cgtcatcttc acaagcaatc
7321 cagctgagta tgccggcgta tatgatacgc aagatgcgct tctcgacagg ttaattacca
7381 tgtttattga ttataaagac atcgacagag agacagcgat tttaacggag aaaacggacg
7441 tagaagaaga tgaagcgcgc acaattgtaa cgctcgtagc aaacgtgcga aaccgctctg
7501 gagacgaaaa cagcagcgga cttagcctgc gggcttcgct tatgatcgct acccttgcca
7561 cgcagcaaga cattcctatc gatggaagtg acgaagattt tcaaacgtta tgtatcgata
7621 ttttgcatca tccgcttacc aaatgtttgg atgaagaaaa tgcaaaaagc aaagccgaaa
7681 aaatcatttt agaagaatgt aagaatatag acactgaaga aaagtaaagg agcttgaaaa
7741 catgagtgaa acaaacgaaa caggtattta tatttttagc gccattcaaa cggataaaga
7801 cgaagaattt ggcgccgtgg aagtagaagg aacaaaagct gaaacatttt tgattcgcta
7861 caaagacgcg gctatggtag cagctgaagt accgatgaaa atttatcatc ctaatcgcca
7921 aaatttatta atgcatcaaa acgcagtagc agcgattatg gacaagaacg atacggttat
7981 tccaatcagc tttgggaatg tattcaaatc aaaagaagac gtaaaagttc ttttggaaaa
8041 cctttatccg cagtttgaaa agctgtttcc agcgatcaaa ggaaaaattg aagtcggttt
8101 aaaagtaatt gggaaaaaag aatggcttga gaaaaaagta aacgaaaatc ctgaacttga
8161 gaaagtatca gcatccgtaa aaggaaaatc agaagcagcc ggttattatg agcgtattca
8221 acttggagga atggctcaaa agatgtttac ttccctgcaa aaagaagtca agacagatgt
8281 gttttctccg cttgaagaag cagcggaagc agcaaaagca aatgagccaa cgggcgaaac
8341 gatgctttta aacgcgtctt tcttaattaa ccgagaagat gaagcgaagt ttgatgaaaa
8401 agtaaatgaa gcgcatgaaa actggaaaga caaagccgat tttcattaca gcggtccttg
8461 gcctgcttat aattttgtga acattcgcct aaaagtagaa gagaaataac gtgcttcaca
8521 aattagtaac cgcacccatt aaccttgtag tgaaaatcgg cgaaaaagta caggaagaag
8581 ctgataaaca gctatatgac cttccgacga ttcagcaaaa gctcattcag cttcaaatga
8641 tgtttgagct tggtgaaatt ccagaagaag cgtttcaaga aaaagaagat gaattgttaa
8701 tgaggtacga aattgcgaaa cgcagagaaa ttgaacaatg ggaagagcta acacaaaaaa
8761 gaaatgagga atcctagatg ggagaattac tgtatttata cggtttaatt ccaacaaaag
8821 aagcagcagc catagagccg tttccatctt ataaggggtt tgacggagaa cattcactgt
8881 acccaattgc gtttgatcag gtgacggctg tagtttctaa gctggatgct gacacctatt
8941 cagaaaaagt gattcaagaa aaaatggagc aggatatgag ctggctgcag gaaaaagcat
9001 ttcatcatca cgaaacggta gccgctttgt acgaagaatt tacgatcatt ccattaaaat
9061 tttgcaccat ttataaaggt gaagaaagtc tgcaggcagc tattgagatt aacaaagaaa
9121 agatagagaa ttcactgacg ctgcttcaag gaaatgaaga gtggaatgtg aaaatttact
9181 gtgatgatac agagcttaaa aaaggaatca gcgaaacgaa tgaaagcgtg aaagcgaaaa
9241 aacaagaaat tagtcactta tcaccaggaa gacagttttt tgaaaagaaa aaaatagatc
9301 agctgattga aaaagaatta gagcttcaca aaaacaaagt gtgtgaagag atacatgaca
9361 agctaaaaga attatcgctt tatgactctg ttaaaaagaa ttggagcaaa gacgtaactg
9421 gcgcagctga acagatggcg tggaacagcg tgtttcttct cccgtctctg cagattacta
9481 agttcgtaaa cgaaatagaa gagcttcagc aaaggcttga aaataaaggc tggaagtttg
9541 aagtgacggg accatggccg ccctatcatt tctcgagctt tgcgtaaagt gaggaattaa
9601 cattatgtct cttaaacaat ccatggagaa taaagatatt gctcttattg atattttaga
9661 tgtcatttta gataaaggag tcgccattaa aggagactta atcatttcca tagctggcgt
9721 cgatttagtg tatttggatt tgcgggtgct tatttcttcg gttgaaacgc ttgtgcaagc
9781 aaaagaagga aatcacaaac caatcacttc tgaacaattt gataaacaaa aggaggaatt
9841 aatggatgca accggtcagc caagcaaatg gacgaatcca cttggatcct gatcaagctg
9901 aacaaggctt agcgcagctt gtgatgacag ttattgagct attgaggcaa atcgttgaac
9961 gtcatgccat gaggcgggtg gagggtggaa cgttgacgga cgaacaaatt gaaaacttag
10021 gaattgcact aatgaactta gaagaaaaaa tggacgagtt gaaagaggtg ttcggtctgg
10081 atgcagaaga tttaaatatt gatcttggac cgctaggcag cctgctttaa gcggtcagta
10141 ggaggaacag tatggcagtc gaacataata tgcagtcaag tacgattgta gatgtgctcg
10201 aaaagatttt ggataaagga gtcgttatag cgggggacat caccgtagga attgcagatg
10261 tcgagctatt aacgataaag atccgcttga ttgtggcttc ggttgataag gcaaaagaaa
10321 tcggcatgga ctggtgggaa aatgatccgt atctcagttc aaaaggagcc aataacaaag
10381 cgctcgaaga agaaaataaa atgctgcatg agcggttaaa aacgcttgaa gaaaaaatag
10441 aaacgaaacg ttaaaaactg tacgctactt aaaaaatgga gggatttaca atggcaactg
10501 aaacaaaatt agataacaca caggcagaaa acaaggaaaa taaaaatgcg gaaaacggtt
10561 caaaagaaaa gaacggttca aaagcaagca aaacaacaag cagcgggcca atcaaacgag
10621 cggtagcagg aggcatcatc ggtgcaacga ttggatatgt atcgactcct gaaaatcgaa
10681 aaagtctcct tgaccgcatt gatacagacg aattaaaaag caaagcatct gatttaggaa
10741 caaaggtaaa agaaaaatca aaaagcagcg tggccagcct gaaaacatct gcgggaagct
10801 tgtttaaaaa agataaagat aaatcaaaag atgatgaaga aaacgtaaat tcttctagta
10861 gcgaaacaga agacgataac gttcaagagt acgacgagtt aaaagaagaa aatcaaactc
10921 ttcaagatcg cttatcacag cttgaagaaa aaatgaacat gcttgttgag cttagcctca
10981 ataaaaatca agacgaagaa gcggaagata cagattccga cgaagaagag aacgatgaga
11041 acgatgaaaa cgatgaaaac gagcaggacg atgaaaacga agaagaaaca tctaagccac
11101 gtaaaaagga taaaaaagaa gctgaggaag aagaaagtga aagtgacgaa gacagcgagg
11161 aagaagagga agattctcgc tcaaacaaaa aaaataaaaa agtaaaaaca gaagaagaag
11221 acgaagatga aagcgaagaa gaaaaaaagg aagcgaaacc aaaaaagtca acagctaaaa
11281 aatcaaaaaa tacaaaagca aagaaaaaca cggacgaaga agatgatgaa gcaacatctc
11341 tttctagtga agacgataca acagcctaag acgtaaagga ggaaagaaag acatgagtac
11401 aggcccttct ttttcaacta aagacaatac gcttgaatac tttgtgaaag cttctaataa
11461 acacggcttt tcacttgata tttcattaaa tgtaaacggc gctgtgattt ccggtaccat
11521 gatttcagca aaagaatact ttgattactt aagcgaaacg tttgaagaag gcagtgaagt
11581 ggctcaggcg ctaagcgaac aattctcttt agcaagcgaa gcgagcgaat caaacggaga
11641 agcagaagcc cattttattc atttgaaaaa tacaaagatt tactgtggag acagtaaatc
11701 tactccttct aaaggcaaaa tcttttggag agggaaaata gcagaagtag acgggttttt
11761 cttaggaaag atttctgatg caaaatcaac gagtaaaaag agttcataaa aaacggcggg
11821 gtgattgccc cgccgttttt tagtgatgtg atgagatgtg cagcttcttt tttcgataca
11881 tgagcggagt catcttcatt tcttttcgaa actgattaat aaaatagctt gtactattaa
11941 accctacttg ataggcgact tccgtcacat ttgcttctgc ttgctgcagg atccggctgc
12001 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
12061 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
12121 cggat
//
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pET28a_T7-ARG1质粒载体应用举例
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