pAG001 His6-TwinStrep-SUMO-AapCas12b

LOCUS       pAG001 His6-TwinStrep-SUMO-AapCas12b                9473 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Bacterial expression plasmid for AapCas12b.
ACCESSION   .
VERSION     .
KEYWORDS    pAG001 His6-TwinStrep-SUMO-AapCas12b
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Joung J, Ladha A, Saito M, Segel M, Bruneau R, Huang MW, Kim NG, Yu 
            X, Li J, Walker BD, Greninger AL, Jerome KR, Gootenberg JS, 
            Abudayyeh OO, Zhang F
  TITLE     Point-of-care testing for COVID-19 using SHERLOCK diagnostics.
  JOURNAL   medRxiv. 2020 May 8. doi: 10.1101/2020.05.04.20091231.
  PUBMED    32511521
REFERENCE   2  (bases 1 to 9473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9473
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             127..144
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             157..240
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             241..531
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIG"
     primer_bind     complement(3974..3992)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3988..4035
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(4210..4238)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(4265..4283)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4351..4455
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4456..5316
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4674..4693)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5487..6075
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5976..5995
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6229..6246
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6261..6400
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6326..6345)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6486..6508
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6502..6693)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8005..9087)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9049..9068
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9088..9165)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9371..9390
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc
      121 agcggcctgg tgccgcgcgg cagccatatg gctagctgga gccatccgca gtttgaaaaa
      181 ggtggtggta gcggtggtgg ttcaggtggt agtgcatggt cacaccctca gtttgagaaa
      241 atgtcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct
      301 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa
      361 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg
      421 gactccttaa gattcttgta cgacggtatt agaatccaag ctgatcagac ccctgaagat
      481 ttggacatgg aggataacga tattattgag gctcacagag aacagattgg tggatccatg
      541 gccgtaaaat ctatgaaagt taagttgcgt ctggacaaca tgccagaaat tcgcgctggc
      601 ctttggaaac tgcacaccga ggtcaatgca ggcgttcgtt attataccga gtggttaagt
      661 ttattgcgtc aggaaaattt ataccgtcgc agtccaaacg gggacgggga acaggagtgt
      721 tacaagacag ctgaggagtg caaggcagag ttgcttgagc gcttgcgcgc acgccaagtt
      781 gagaatggac actgcggtcc ggctgggtca gacgatgagc ttcttcaatt ggcacgtcag
      841 ttatacgaat tattagtacc ccaggcgatc ggtgcgaaag gcgacgctca gcagatcgcg
      901 cgtaaattcc tgtccccgct ggctgataaa gatgctgtcg gtggcctggg aattgccaaa
      961 gccgggaata aaccgcgttg ggtccgtatg cgcgaggctg gagagccggg atgggaagaa
     1021 gagaaggcaa aagcggaagc ccgtaaatcc acggaccgta ctgcagacgt cttgcgcgcc
     1081 ttagccgatt ttgggcttaa accgttgatg cgcgtctaca ccgactcaga tatgtcgtct
     1141 gttcaatgga agcccctgcg taaagggcag gccgtacgta cctgggatcg tgatatgttt
     1201 cagcaggcga tcgagcgtat gatgtcctgg gaatcctgga accaacgcgt aggcgaagca
     1261 tacgcaaaat tggtcgaaca aaagtcgcgc ttcgagcaga agaactttgt cggacaggaa
     1321 catctggttc aattggtcaa ccagctgcaa caggacatga aggaagcatc tcacggtctg
     1381 gaatcgaaag agcaaactgc acactatctt actggtcgtg cgttgcgtgg tagtgataaa
     1441 gtgtttgaga agtgggagaa gcttgatccc gatgcgcctt ttgatcttta cgacaccgag
     1501 attaagaacg ttcaacgtcg caatacacgt cgctttggtt cgcatgactt attcgccaag
     1561 ttggcagaac cgaaatacca agccctgtgg cgtgaagacg cgagctttct tacgcgttat
     1621 gcagtgtaca atagtattgt ccgcaaactg aaccacgcca aaatgtttgc aacctttacg
     1681 cttccagacg ccacagctca ccctatttgg acccgctttg ataaattggg gggtaatctt
     1741 caccaatata cctttctgtt caacgagttc ggtgagggtc gccatgctat ccgcttccaa
     1801 aagttactga cggtggaaga cggcgtggca aaggaagttg atgacgtgac tgtcccgatc
     1861 agcatgtcag cccagttaga cgacctgctg ccgcgcgatc cgcatgaatt ggtcgcgctt
     1921 tatttccaag attatggcgc ggaacaacac ttagcgggtg aattcggtgg ggcaaaaatc
     1981 caatatcgtc gcgaccagct taaccacctg cacgctcgcc gcggtgcccg cgatgtttat
     2041 ctgaaccttt cggtacgtgt gcagagtcaa tcagaggcac gcggcgaacg tcgtcctcca
     2101 tatgctgcag tctttcgctt ggtaggggat aatcaccgcg catttgtgca tttcgataag
     2161 ttatctgact accttgcaga acatccggat gatggtaaat tgggctcgga gggtctttta
     2221 tcaggcttac gcgtaatgag tgtagacctg ggcctgcgta cttcagccag catctctgta
     2281 tttcgcgtcg cacgtaaaga cgagttaaaa ccgaattccg agggtcgcgt tcctttctgc
     2341 ttccctattg aagggaacga gaacctggta gcggttcacg aacgctccca attgttgaag
     2401 ctgcccggag agacagaatc gaaggatctg cgtgctatcc gtgaggagcg ccagcgtacg
     2461 ttgcgccaat tacgtacaca gcttgcatat ttgcgtcttt tagtacgctg tggttcagag
     2521 gatgtgggtc gccgtgaacg ttcctgggca aagctgatcg aacaaccgat ggatgctaat
     2581 cagatgaccc ccgactggcg cgaagctttc gaggacgagc tgcagaaact taagtcattg
     2641 tatggtatct gtggagatcg tgaatggact gaggcagtct acgaatctgt gcgccgcgta
     2701 tggcgccata tgggaaagca ggtacgcgac tggcgtaagg atgttcgttc cggcgaacgc
     2761 cctaagattc gcggctatca gaaagacgtt gttggtggca attcgattga acagattgag
     2821 tatcttgaac gtcagtacaa atttctgaag tcttggtcat tctttggaaa agtatcaggt
     2881 caggtaattc gtgccgagaa ggggtcccgc ttcgctatca ccttacgcga acatatcgat
     2941 catgcgaaag aagaccgcct taaaaaattg gctgatcgta tcatcatgga ggcattagga
     3001 tatgtgtatg cactggacga cgaacgtggc aaagggaaat gggttgccaa gtatccgccg
     3061 tgtcaattga ttttactgga agaattgtcg gagtaccagt ttaacaacga ccgtcccccc
     3121 tcagagaata accagcttat gcaatggtcg catcgtggag tgttccaaga actgttaaac
     3181 caagcacaag ttcatgacct tcttgtaggt actatgtatg cggcattttc cagtcgcttt
     3241 gacgcacgta cgggggctcc ggggatccgt tgtcgtcgcg tacctgcacg ttgcgcacgc
     3301 gaacagaatc cggagccttt tccttggtgg ttgaataagt tcgttgcgga gcataagttg
     3361 gacggctgcc ctttgcgcgc tgatgacctg atccccacag gcgaaggtga gttcttcgtt
     3421 agtccatttt cagctgagga gggtgacttt caccagatcc atgccgacct taacgccgcc
     3481 caaaatttgc aacgtcgtct ttggtcagac tttgatatct cgcaaattcg tctgcgctgt
     3541 gattgggggg aggttgatgg cgagccagtt ttaattccgc gcacaactgg caagcgtacc
     3601 gctgacagct acggcaacaa ggttttttat accaagaccg gtgtaacgta ttacgaacgt
     3661 gagcgcggta aaaaacgtcg caaagttttt gctcaggaag aactgagtga agaagaggct
     3721 gaacttttgg tggaagccga tgaggcccgt gagaaatcag tagttttgat gcgtgatccg
     3781 tcaggaatca ttaatcgtgg agactggacg cgtcaaaaag aattttggag tatggtcaac
     3841 caacgcatcg agggttacct tgttaagcag atccgctcgc gcgtccgtct gcaggaaagt
     3901 gcttgtgaga acactgggga catctgagcg gccgcactcg aggcccgaaa ggaagctgag
     3961 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     4021 ttgaggggtt ttttgctgaa aggaggaact atatccggat atcccgcaag aggcccggca
     4081 gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga
     4141 tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata
     4201 aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga
     4261 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     4321 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4381 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4441 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4501 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4561 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4621 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4681 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     4741 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4801 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4861 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4921 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4981 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5041 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5101 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5161 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5221 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5281 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5341 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5401 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5461 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5521 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5581 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     5641 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5701 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5761 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5821 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5881 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5941 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6001 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6061 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6121 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     6181 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     6241 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     6301 tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     6361 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     6421 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     6481 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     6541 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc
     6601 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
     6661 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
     6721 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     6781 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
     6841 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg
     6901 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg
     6961 cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt
     7021 tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga
     7081 ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag
     7141 cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct
     7201 gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt
     7261 tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg
     7321 ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg
     7381 cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag
     7441 gcggcataaa tcgccgtgac gatcagcggt ccaatgatcg aagttaggct ggtaagagcc
     7501 gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc
     7561 tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc
     7621 cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg
     7681 ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg
     7741 agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga
     7801 aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg
     7861 ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag
     7921 ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc
     7981 taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     8041 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag
     8101 ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc
     8161 ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt
     8221 gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac
     8281 cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc
     8341 catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat
     8401 ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat
     8461 ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact
     8521 taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc
     8581 cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac
     8641 atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc
     8701 atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc
     8761 cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag
     8821 ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact
     8881 ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt
     8941 gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac
     9001 gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc
     9061 gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg
     9121 ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct
     9181 ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca
     9241 ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg
     9301 ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat
     9361 cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga
     9421 tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
//