| 质粒名称: | lenti.DTR.GFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 201962 |
| 存储实验室: | Bernhard Schmierer |
|
载体骨架基本信息 |
|
| 载体名称: | lenti.Cas9.BFP.Blast, #196714 |
| 载体出品公司: | Clontech |
| 空载体大小: | 5738bp |
| 完整质粒大小: | 10590 |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 筛选标记: | EGFP |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | Diptheria Toxin Receptor、DTR, HBEGF |
| 物种来源: | Chlorocebus sabaeus |
| 插入大小: | 1362bp |
| GenBank编号 | XM_008014669.2 |
| 启动子: | EF1alpha |
| 标签/融合蛋白: | EGFP (C terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BsiWI (destroyed during cloning) |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物 | tctagaactagtggatc |
| 3’测序引物 | GATACCGTCGACCTCGA |
|
文献引用方法 |
|
| 材料和方法部分: | lenti.DTR.GFP was a gift from Bernhard Schmierer (Addgene plasmid # 201962 ; http://n2t.net/addgene:201962 ; RRID:Addgene_201962) |
| 参考文献部分: | An idiosyncratic zonated stroma encapsulates desmoplastic liver metastases and originates from injured liver. Fernandez Moro C, Geyer N, Harrizi S, Hamidi Y, Soderqvist S, Kuznyecov D, Tidholm Qvist E, Salmonson Schaad M, Hermann L, Lindberg A, Heuchel RL, Martin-Bernabe A, Dhanjal S, Navis AC, Villard C, Del Valle AC, Bozoky L, Sparrelid E, Dirix L, Strell C, Ostman A, Schmierer B, Vermeulen PB, Engstrand J, Bozoky B, Gerling M. Nat Commun. 2023 Aug 18;14(1):5024. doi: 10.1038/s41467-023-40688-x. 10.1038/s41467-023-40688- |
买家导航
lenti.DTR.GFP载体质粒基本信息
lenti.DTR.GFP质粒图谱载体图谱和 lenti.DTR.GFP载体序列质粒序列多克隆位点信息
lenti.DTR.GFP质粒载体简介
lenti.DTR.GFP质粒序列载体序列
LOCUS lenti.DTR.GFP 10590 bp DNA circular SYN 15-DEC-2025
DEFINITION Lentiviral vector that expresses diptheria toxin receptor (DTR)
C-terminally fused to EGFP.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10590)
AUTHORS Fernandez Moro C, Geyer N, Harrizi S, Hamidi Y, Soderqvist S,
Kuznyecov D, Tidholm Qvist E, Salmonson Schaad M, Hermann L,
Lindberg A, Heuchel RL, Martin-Bernabe A, Dhanjal S, Navis AC,
Villard C, Del Valle AC, Bozoky L, Sparrelid E, Dirix L, Strell C,
Ostman A, Schmierer B, Vermeulen PB, Engstrand J, Bozoky B, Gerling
M
TITLE An idiosyncratic zonated stroma encapsulates desmoplastic liver
metastases and originates from injured liver.
JOURNAL Nat Commun. 2023 Aug 18;14(1):5024. doi: 10.1038/s41467-023-40688-x.
PUBMED 37596278
REFERENCE 2 (bases 1 to 10590)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat
Commun."; date: "2023-08-18"; volume: "14(1):5024. doi"; pages: "
10.1038/s41467-023-40688-x"
COMMENT Sequence Label: lenti.DTR.GFP
FEATURES Location/Qualifiers
source 1..10590
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(19..607)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(99..118)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(778..1569,1570..1638))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1401..1420
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1639..1743)
/gene="bla"
/label=AmpR promoter
primer_bind complement(1818..1837)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 2009..2388
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2390..2588
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2543..2563
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 2606..2786
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 2833..2958
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 3451..3684
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 3869..3913
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 4211..4328
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 4458..5636
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 4689..5627
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 5584..5604
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
regulatory 6295..6304
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS join(6301..6303,6304..6306,6307..7020)
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS join(6301..6303,6304..6306,6307..7017)
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(6346..6367)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(6607..6626)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 6954..6975
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 7053..7641
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(7106..7126)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(7524..7535)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(7644..7660)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7645..7661)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 8166..8346
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(8372..8389)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 8378..8602
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 8648..9076
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8735..8754)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8945..8966
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(9085..9105)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9090..9419
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 9270..9405
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9332..9351
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 9467..9514
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 9533..9907
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 10037..10158
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10074..10093)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10128..10147
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(10207..10223)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10207..10223)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(10220..10242)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 10231..10247
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(10255..10261,10262..10279,10280..10285))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 10300..10321
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(10438..10455)
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
61 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
121 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
181 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
241 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
301 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
361 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
421 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
481 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
541 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
601 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
661 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
721 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
781 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
841 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
901 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
961 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
1021 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
1081 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
1141 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
1201 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
1261 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
1321 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
1381 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
1441 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
1501 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
1561 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
1621 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
1681 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
1741 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
1801 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
1861 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
1921 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
1981 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
2041 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
2101 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
2161 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
2221 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
2281 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
2341 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
2401 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
2461 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
2521 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
2581 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
2641 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
2701 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
2761 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
2821 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
2881 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
2941 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
3001 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
3061 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
3121 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
3181 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
3241 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
3301 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
3361 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
3421 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
3481 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
3541 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
3601 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
3661 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
3721 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
3781 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
3841 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
3901 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
3961 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
4021 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
4081 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
4141 cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat
4201 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
4261 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
4321 aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt agctagctgc
4381 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
4441 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
4501 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
4561 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
4621 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
4681 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
4741 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
4801 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
4861 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
4921 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
4981 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
5041 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
5101 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
5161 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
5221 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
5281 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
5341 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
5401 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
5461 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
5521 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
5581 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgacctg
5641 caggaattcg ccactatgaa gctgctgccg tcggtggtgc tgaagctcct tctggctgca
5701 gttctttcgg cactggtgac tggcgagagc ctggagcagc ttcggagagg gctagctgct
5761 ggaaccagca acccggaccc ttccactgga tctacggacc agctgctacg cctaggaggc
5821 ggccgggacc ggaaagtccg tgacttgcaa gaggcagatc tggacctttt gagagtcact
5881 ttatcctcca agccacaagc actggccaca ccaagcaagg aggagcacgg gaaaagaaag
5941 aagaaaggca agggactagg gaagaagagg gacccatgtc ttcggaaata caaggacttc
6001 tgcatccacg gagaatgcaa atatgtgaag gagctccggg ctccctcctg catctgccac
6061 ccaggttacc atggagagag gtgtcatggg ctgagcctcc cagtggaaaa tcgcttatat
6121 acctatgacc atacaactat cctggctgtg gtggccgtgg tgctgtcctc tgtctgtctg
6181 ctggtcatcg tggggcttct catgtttagg taccatagga gaggtggtta tgatgtggaa
6241 aacgaagaga aagtgaagtt gggcatgact aattcccacc cggatccacc ggtcgccacc
6301 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
6361 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
6421 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
6481 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
6541 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
6601 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
6661 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
6721 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
6781 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
6841 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
6901 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
6961 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
7021 agcggccgga attcgatatc aagcttatcg gtaatcaacc tctggattac aaaatttgtg
7081 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
7141 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
7201 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
7261 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
7321 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
7381 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
7441 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
7501 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
7561 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
7621 ccctttgggc cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag
7681 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
7741 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
7801 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
7861 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
7921 ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt
7981 gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca
8041 cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag
8101 agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact
8161 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
8221 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
8281 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
8341 ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
8401 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
8461 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
8521 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
8581 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
8641 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
8701 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
8761 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
8821 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
8881 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
8941 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
9001 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
9061 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
9121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
9181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
9241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
9301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
9361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
9421 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
9481 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
9541 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
9601 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
9661 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
9721 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
9781 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
9841 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
9901 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
9961 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
10021 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
10081 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
10141 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
10201 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
10261 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
10321 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
10381 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
10441 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
10501 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
10561 aggccagcaa aaggccagga accgtaaaaa
//
